Rroxscaffold_4G00320520

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005021
Physical Location & Seq
Reverse (-)
49211387 .. 49217828
6442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320520.1

Sequence Viewer

Length: 489 bp
ATGTTTGTGGACCATGAAATGTATTTTGAAAAATGTGCAGACTTTGCCAAATCCTTCCTCCAACTATATATCAAAGAGGATGATGTATTATTCAGAGTGCTCTTGCAATTGCTTTGTGTACCTTGTCCTGCAGAGAAACAGTTTGAAGAGGAGAGGGGATCATTTCAGGATTCAAAGGGCAATGTGCTTTTCTTTGTTTCAGATCTTTTCAATCCTGTACTCTTATTCCATCTATTTCTTGTAGAGTTAAGCTATGACCATCAGGTGCTTCTTGATTACCTTATTACAAAAGATACTGGAATCAGTTGTGCAGAATATCTTTTAAGGTGCTTGCGTAAAGTGTGTGATTCATGGATCTTATTTGTGGAATTTCCACCGAGTGGACAAGCTATTAATCGATCATCTTGCAAGAAAAGAAAAGTTTCATTGAATGGGTCGAGTTTCTGTGGAGCAGATTTGTTTGCGCTAGTGAAAGACTCTGGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LINES_C LINES_N

Protein Analysis

162

Amino Acids

18.56

Weight (kDa)

5.18

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LINES_N PF14694 2 - 123 6.1e-09 Lines N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 380
AclWI GGATC 2 cut(s) 166, 362
AcsI RAATTY 1 cut(s) 368
AdeI CACNNNGTG 1 cut(s) 380
AfaI GTAC 2 cut(s) 120, 219
AfiI CCNNNNNNNGG 1 cut(s) 380
AgsI TTSAA 5 cut(s) 29, 146, 174, 211, 430
AluBI AGCT 3 cut(s) 252, 389, 485
AluI AGCT 3 cut(s) 252, 389, 485
Alw21I GWGCWC 1 cut(s) 102
AlwI GGATC 2 cut(s) 166, 362
ApoI RAATTY 1 cut(s) 368
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 421
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 10
AvaII GGWCC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 2 cut(s) 237, 267
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 129
BglII AGATCT 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 10
BmgT120I GGNCC 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 397
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 187
BseCI ATCGAT 1 cut(s) 397
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 187
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 164
BsgI GTGCAG 2 cut(s) 57, 330
BshVI ATCGAT 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 4 cut(s) 158, 202, 354, 398
BspDI ATCGAT 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 2 cut(s) 166, 362
BsrDI GCAATG 1 cut(s) 187
BsrI ACTGG 1 cut(s) 301
BssMI GATC 4 cut(s) 158, 202, 354, 398
Bst4CI ACNGT 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 332
BstF5I GGATG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 4 cut(s) 161, 205, 357, 401
BstMBI GATC 4 cut(s) 158, 202, 354, 398
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 202, 354
BstYI RGATCY 2 cut(s) 202, 354
Bsu15I ATCGAT 1 cut(s) 397
BsuTUI ATCGAT 1 cut(s) 397
BtsCI GGATG 1 cut(s) 85
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 397
Csp6I GTAC 2 cut(s) 119, 218
CviAII CATG 2 cut(s) 14, 351
CviJI RGCY 3 cut(s) 252, 389, 485
CviKI_1 RGCY 3 cut(s) 252, 389, 485
CviQI GTAC 2 cut(s) 119, 218
DpnI GATC 4 cut(s) 160, 204, 356, 400
DpnII GATC 4 cut(s) 158, 202, 354, 398
DraIII CACNNNGTG 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 10
FaeI CATG 2 cut(s) 17, 354
FaiI YATR 5 cut(s) 15, 67, 69, 255, 352
FatI CATG 2 cut(s) 13, 350
FokI GGATG 1 cut(s) 92
FspBI CTAG 1 cut(s) 467
GlaI GCGC 1 cut(s) 465
HhaI GCGC 1 cut(s) 466
Hin1II CATG 2 cut(s) 17, 354
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HinfI GANTC 4 cut(s) 170, 300, 347, 476
Hpy166II GTNNAC 3 cut(s) 10, 119, 383
Hpy188I TCNGA 2 cut(s) 95, 202
Hpy188III TCNNGA 3 cut(s) 167, 272, 480
Hpy8I GTNNAC 3 cut(s) 10, 119, 383
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 5 cut(s) 38, 106, 131, 311, 408
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 17, 354
HspAI GCGC 1 cut(s) 464
Kzo9I GATC 4 cut(s) 158, 202, 354, 398
LmnI GCTCC 2 cut(s) 449, 482
LpnPI CCDG 6 cut(s) 141, 152, 228, 248, 282, 465
MaeI CTAG 1 cut(s) 467
MalI GATC 4 cut(s) 160, 204, 356, 400
MboI GATC 4 cut(s) 158, 202, 354, 398
MboII GAAGA 1 cut(s) 158
MfeI CAATTG 1 cut(s) 107
MflI RGATCY 2 cut(s) 202, 354
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 2 cut(s) 107, 368
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 4 cut(s) 68, 70, 142, 147
MroXI GAANNNNTTC 1 cut(s) 421
MseI TTAA 4 cut(s) 248, 323, 393, 487
MunI CAATTG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 4 cut(s) 158, 202, 354, 398
NlaIII CATG 2 cut(s) 17, 354
PdmI GAANNNNTTC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 170, 300, 347
PflMI CCANNNNNTGG 1 cut(s) 380
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 393
PspPI GGNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 133
PsuI RGATCY 2 cut(s) 202, 354
RsaI GTAC 2 cut(s) 120, 219
RsaNI GTAC 2 cut(s) 119, 218
SaqAI TTAA 4 cut(s) 248, 323, 393, 487
Sau3AI GATC 4 cut(s) 158, 202, 354, 398
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 1 cut(s) 470
SduI GDGCHC 1 cut(s) 102
SetI ASST 7 cut(s) 124, 254, 267, 282, 329, 391, 487
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 1 cut(s) 10
Sse9I AATT 2 cut(s) 107, 368
SspMI CTAG 1 cut(s) 467
TaaI ACNGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 397, 437
TasI AATT 2 cut(s) 107, 368
TatI WGTACW 1 cut(s) 217
TfiI GAWTC 3 cut(s) 170, 300, 347
Tru1I TTAA 4 cut(s) 248, 323, 393, 487
Tru9I TTAA 4 cut(s) 248, 323, 393, 487
TspDTI ATGAA 3 cut(s) 30, 339, 414
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 10
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 421
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.