pycom06g14750

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004983
Physical Location & Seq
Reverse (-)
19420651 .. 19421893
1243 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGGCATCGCATGGGATTTTGTTGGCTTCACTTCTTGTGCTCTCTTGCTTATCCCAAACCTCCTATGGGATTGTCCTCTTTTCTTCTCTCAAGGATAGTCTATTTGTCACTGCTTCACACACAGAAAACCAGGTTCTGAAGGCAGGGATAGACAAGCTCACCGTTTCATGGGGTCTAAACCAGAGCTACGAAGCCGGCACCGACTCAACCTTCAAGACCATCAAGGTCAAGTTATGCTTCGCGCAGGTGAGCCAAGTCGACCGCGGATGGAGGAAGACCGTCGACAACCTCGCCATGGACAAGACATGCCTGTTCAAGATCGTCGAAAGGCCGTACACCAGCAACAAGTCCAACGACAGCTTCAACTGA

Protein Analysis

123

Amino Acids

13.47

Weight (kDa)

8.82

Isoelectric Point (pI)

13.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 27 - 118 3.4e-35 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 197
AccI GTMKAC 2 cut(s) 258, 282
AccII CGCG 2 cut(s) 242, 264
AciI CCGC 2 cut(s) 262, 264
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 3 cut(s) 66, 168, 295
AgsI TTSAA 3 cut(s) 214, 316, 364
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 1 cut(s) 344
AluBI AGCT 3 cut(s) 157, 186, 360
AluI AGCT 3 cut(s) 157, 186, 360
Alw21I GWGCWC 1 cut(s) 42
AlwNI CAGNNNCTG 1 cut(s) 136
AoxI GGCC 1 cut(s) 329
AspLEI GCGC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 151, 259
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 42
BccI CCATC 2 cut(s) 227, 261
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 262, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 168, 295
Bse118I RCCGGY 1 cut(s) 194
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 262, 294
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 3 cut(s) 66, 168, 295
Bsh1236I CGCG 2 cut(s) 242, 264
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 3 cut(s) 66, 168, 295
BsnI GGCC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 262, 264
BspANI GGCC 1 cut(s) 331
BspFNI CGCG 2 cut(s) 242, 264
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 262, 294
BssMI GATC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 163, 280
BstC8I GCNNGC 1 cut(s) 196
BstDSI CCRYGG 2 cut(s) 262, 294
BstF5I GGATG 1 cut(s) 272
BstFNI CGCG 2 cut(s) 242, 264
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 1 cut(s) 321
BstMBI GATC 1 cut(s) 318
BstMCI CGRYCG 1 cut(s) 262
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 2 cut(s) 242, 264
BstV2I GAAGAC 1 cut(s) 281
BsuRI GGCC 1 cut(s) 331
BtgI CCRYGG 2 cut(s) 262, 294
BtsCI GGATG 1 cut(s) 272
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 194
Cfr42I CCGCGG 1 cut(s) 265
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 11, 168, 295, 306
CviJI RGCY 7 cut(s) 26, 157, 186, 194, 252, 331, 360
CviKI_1 RGCY 7 cut(s) 26, 157, 186, 194, 252, 331, 360
CviQI GTAC 1 cut(s) 334
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 4 cut(s) 14, 171, 298, 309
FaiI YATR 6 cut(s) 12, 66, 169, 235, 296, 307
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FatI CATG 4 cut(s) 10, 167, 294, 305
FblI GTMKAC 2 cut(s) 258, 282
FokI GGATG 1 cut(s) 279
GlaI GCGC 1 cut(s) 243
HaeIII GGCC 1 cut(s) 331
HapII CCGG 1 cut(s) 195
HhaI GCGC 1 cut(s) 244
Hin1II CATG 4 cut(s) 14, 171, 298, 309
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 2 cut(s) 259, 283
HindII GTYRAC 2 cut(s) 259, 283
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 151, 259
Hpy166II GTNNAC 3 cut(s) 259, 283, 336
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 2 cut(s) 214, 316
Hpy8I GTNNAC 3 cut(s) 259, 283, 336
Hpy99I CGWCG 2 cut(s) 284, 326
HpyAV CCTTC 2 cut(s) 133, 220
HpyCH4III ACNGT 2 cut(s) 163, 280
Hsp92II CATG 4 cut(s) 14, 171, 298, 309
HspAI GCGC 1 cut(s) 242
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 265
Kzo9I GATC 1 cut(s) 318
LpnPI CCDG 8 cut(s) 116, 129, 143, 194, 208, 230, 323, 352
LweI GCATC 1 cut(s) 14
MabI ACCWGGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 2 cut(s) 75, 286
MhlI GDGCHC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 4 cut(s) 70, 86, 264, 299
MroNI GCCGGC 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 2 cut(s) 242, 264
NaeI GCCGGC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 294
NdeII GATC 1 cut(s) 318
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 4 cut(s) 14, 171, 298, 309
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 1 cut(s) 309
PaqCI CACCTGC 1 cut(s) 235
PdiI GCCGGC 1 cut(s) 196
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacII CCGCGG 1 cut(s) 265
SalI GTCGAC 2 cut(s) 257, 281
Sau3AI GATC 1 cut(s) 318
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 42
SetI ASST 9 cut(s) 62, 135, 159, 188, 212, 228, 249, 291, 362
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 1 cut(s) 14
Sfr303I CCGCGG 1 cut(s) 265
SgrBI CCGCGG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SsiI CCGC 2 cut(s) 262, 264
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 163, 280
TaqI TCGA 3 cut(s) 258, 282, 324
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 1 cut(s) 115
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XmiI GTMKAC 2 cut(s) 258, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.