pycom06g20360

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003258
Physical Location & Seq
Reverse (-)
23436022 .. 23438824
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGAATGGTGAATTTGATACCAAATTAGGTTCGGGTGATTTGGACACCCAGAACCCCACACAGTTGGGATCACAAACCCGACTTAAACCCTTCTTTCTCCCTTTCTCCGGAGTATTCTCCCACAGCAAATCGATCGGCTTTCTGCTTTTGCTTTCAAGCCGGTCCAGCATGAGAAGGGCTTCCACTCTCGCCTCACTCCCCCTCCTCTCCCGATCCCTCTCCACCGTCCACGGCGGCGCCACTGCCACGTCAGCCCCCGCCACCTACGGTCTTCTCCAGGTTGCTCTGTACGGGACCACCTCCACCGGTTCTCAGGCTCGTCGTCGATGGCTCGATTCGTTTTCCGGAGGTTCGGGTCGGCAGCTGGCAATTGGCGTCGCCGGAACTCTGGCCTCGGTCGCTGTCGTCACGTCGCTCGCTCCGGAGGTCTATGCGAAAGAGCCGCCTCCGGCTGCGCTTGTTCCCAAGGAGGTCGTGCTTTACCAGTACGAAGCATGCCCTTTCTGCAACAAAGTTAAAGCATTCTTGGATTACCACGATATACCCTACAAAGTTGTGGAGGTCAACCCACTTAGTAAGAAAGAGATTAAATGGTCCGATTATAAGAAGGTTCCGATACTGATGGTGGATGGGGAGCAGCTGGTTGACTCATCAGCTATAATTGATCAGTTAAATAGCAGAATTGTTCCTGAGAGAGCAGCTGCTTCTTCTTCAGATGACGATGAAGAGAAAAAGTGGCGCCAGTGGGTTGATAATCACTTGGTGCACATGTTGTCACCAAACATATACCGAAATACATCGGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTTAGCTACACCGAAAAAATTGCAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTGTCAAAGAAATTGAAGAAGAAATATAATATTACTGATGAACGTGCCTCCCTGTATGAAGCAGCAGAAACATGGGTTGATGCTCTTAATGGGCGGGACTTCCTCGGGGGCTCCAAACCTAATTTGGCCGACCTTGCTGTTTTTGGGGTGCTAAGACCAATCCGTTATTTGAGGTCGGGTAAAGATATGGTGGAGCACACACGCATCGGTGAATGGTACTCAAGAATGGAGCAAGCCGTGGGAGAGCCTGCAAGGATTAAGGCGTAA

Protein Analysis

389

Amino Acids

42.74

Weight (kDa)

8.9

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 158 - 212 3.7e-11 Glutaredoxin
GST_N_3 PF13417 160 - 226 2.7e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 165 - 226 1.3e-08 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 167 - 258 6.5e-10 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccB1I GGYRCC 2 cut(s) 236, 738
AccIII TCCGGA 3 cut(s) 107, 344, 421
AciI CCGC 4 cut(s) 234, 258, 443, 994
AclWI GGATC 2 cut(s) 76, 207
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 696
AcyI GRCGYC 3 cut(s) 237, 375, 739
AdeI CACNNNGTG 1 cut(s) 763
AfaI GTAC 4 cut(s) 290, 488, 896, 1118
AfiI CCNNNNNNNGG 1 cut(s) 107
AflIII ACRYGT 1 cut(s) 768
AgeI ACCGGT 1 cut(s) 305
AgsI TTSAA 2 cut(s) 156, 916
AjiI CACGTC 2 cut(s) 249, 411
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 6 cut(s) 364, 640, 656, 701, 849, 890
AluI AGCT 6 cut(s) 364, 640, 656, 701, 849, 890
Alw21I GWGCWC 2 cut(s) 768, 1098
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 2 cut(s) 76, 207
Ama87I CYCGRG 1 cut(s) 1004
Aor13HI TCCGGA 3 cut(s) 107, 344, 421
AoxI GGCC 3 cut(s) 390, 804, 1026
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 8 cut(s) 361, 452, 637, 698, 701, 884, 887, 962
ApoI RAATTY 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 3 cut(s) 239, 457, 741
AspS9I GGNCC 4 cut(s) 162, 294, 594, 805
AsuHPI GGTGA 4 cut(s) 20, 47, 768, 1121
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 3 cut(s) 162, 294, 594
BaeGI GKGCMC 1 cut(s) 768
BanI GGYRCC 2 cut(s) 236, 738
BanII GRGCYC 1 cut(s) 1013
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 2 cut(s) 768, 1098
BbvI GCAGC 8 cut(s) 373, 439, 649, 688, 710, 871, 899, 974
BccI CCATC 3 cut(s) 321, 616, 623
BceAI ACGGC 2 cut(s) 247, 1121
BcgI CGANNNNNNTGC 4 cut(s) 115, 149, 845, 879
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 1 cut(s) 664
BfmI CTRYAG 1 cut(s) 885
BfoI RGCGCY 2 cut(s) 240, 742
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 3 cut(s) 162, 294, 594
BmeT110I CYCGRG 1 cut(s) 1004
BmgBI CACGTC 2 cut(s) 249, 411
BmgT120I GGNCC 4 cut(s) 162, 294, 594, 805
BmiI GGNNCC 5 cut(s) 238, 295, 612, 740, 1012
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 2 cut(s) 970, 1113
BpiI GAAGAC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 260
BpuEI CTTGAG 2 cut(s) 830, 1105
Bsa29I ATCGAT 1 cut(s) 131
BsaHI GRCGYC 3 cut(s) 237, 375, 739
BsaJI CCNNGG 5 cut(s) 229, 393, 465, 1003, 1137
BsaWI WCCGGW 4 cut(s) 107, 305, 344, 421
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 2 cut(s) 159, 305
Bse1I ACTGG 2 cut(s) 484, 742
Bse3DI GCAATG 1 cut(s) 841
BseAI TCCGGA 3 cut(s) 107, 344, 421
BseBI CCWGG 1 cut(s) 278
BseCI ATCGAT 1 cut(s) 131
BseDI CCNNGG 5 cut(s) 229, 393, 465, 1003, 1137
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMI GCAATG 1 cut(s) 841
BseMII CTCAG 2 cut(s) 326, 681
BseNI ACTGG 2 cut(s) 484, 742
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 8 cut(s) 373, 439, 649, 688, 710, 871, 899, 974
Bsh1285I CGRYCG 2 cut(s) 135, 399
BshFI GGCC 3 cut(s) 392, 806, 1028
BshNI GGYRCC 2 cut(s) 236, 738
BshTI ACCGGT 1 cut(s) 305
BshVI ATCGAT 1 cut(s) 131
BsiEI CGRYCG 2 cut(s) 135, 399
BsiHKAI GWGCWC 2 cut(s) 768, 1098
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 7 cut(s) 108, 160, 306, 345, 381, 422, 449
BslFI GGGAC 2 cut(s) 307, 1010
BslI CCNNNNNNNGG 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 307, 1010
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 3 cut(s) 392, 806, 1028
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 3 cut(s) 768, 1013, 1098
Bsp13I TCCGGA 3 cut(s) 107, 344, 421
Bsp143I GATC 4 cut(s) 68, 132, 212, 664
BspACI CCGC 4 cut(s) 234, 258, 443, 994
BspANI GGCC 3 cut(s) 392, 806, 1028
BspCNI CTCAG 2 cut(s) 325, 682
BspDI ATCGAT 1 cut(s) 131
BspEI TCCGGA 3 cut(s) 107, 344, 421
BspLI GGNNCC 5 cut(s) 238, 295, 612, 740, 1012
BspMAI CTGCAG 1 cut(s) 889
BspPI GGATC 2 cut(s) 76, 207
BspT107I GGYRCC 2 cut(s) 236, 738
BsrDI GCAATG 1 cut(s) 841
BsrFI RCCGGY 2 cut(s) 159, 305
BsrI ACTGG 2 cut(s) 484, 742
BssAI RCCGGY 2 cut(s) 159, 305
BssECI CCNNGG 5 cut(s) 229, 393, 465, 1003, 1137
BssMI GATC 4 cut(s) 68, 132, 212, 664
BssNI GRCGYC 3 cut(s) 237, 375, 739
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 3 cut(s) 63, 226, 269
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 3 cut(s) 237, 375, 739
BstC8I GCNNGC 5 cut(s) 366, 417, 496, 1134, 1149
BstDEI CTNAG 4 cut(s) 312, 572, 690, 1052
BstDSI CCRYGG 2 cut(s) 229, 1137
BstF5I GGATG 1 cut(s) 634
BstH2I RGCGCY 2 cut(s) 240, 742
BstHHI GCGC 3 cut(s) 239, 457, 741
BstKTI GATC 4 cut(s) 71, 135, 215, 667
BstMBI GATC 4 cut(s) 68, 132, 212, 664
BstMCI CGRYCG 2 cut(s) 135, 399
BstMWI GCNNNNNNNGC 6 cut(s) 165, 251, 398, 504, 884, 1034
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 2 cut(s) 498, 772
BstSCI CCNGG 1 cut(s) 276
BstSFI CTRYAG 1 cut(s) 885
BstSLI GKGCMC 1 cut(s) 768
BstV1I GCAGC 8 cut(s) 373, 439, 649, 688, 710, 871, 899, 974
BstV2I GAAGAC 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 131
BsuRI GGCC 3 cut(s) 392, 806, 1028
BsuTUI ATCGAT 1 cut(s) 131
BtgI CCRYGG 2 cut(s) 229, 1137
BtrI CACGTC 2 cut(s) 249, 411
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 2 cut(s) 240, 873
BtsIMutI CAGTG 3 cut(s) 240, 749, 873
Cac8I GCNNGC 5 cut(s) 366, 417, 496, 1134, 1149
CfoI GCGC 3 cut(s) 239, 457, 741
Cfr10I RCCGGY 2 cut(s) 159, 305
Cfr13I GGNCC 4 cut(s) 162, 294, 594, 805
ClaI ATCGAT 1 cut(s) 131
CseI GACGC 1 cut(s) 364
Csp6I GTAC 4 cut(s) 289, 487, 895, 1117
CspAI ACCGGT 1 cut(s) 305
CviAII CATG 4 cut(s) 169, 495, 769, 972
CviQI GTAC 4 cut(s) 289, 487, 895, 1117
DdeI CTNAG 4 cut(s) 312, 572, 690, 1052
DinI GGCGCC 2 cut(s) 238, 740
DpnI GATC 4 cut(s) 70, 134, 214, 666
DpnII GATC 4 cut(s) 68, 132, 212, 664
DraIII CACNNNGTG 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 1013
Eco47I GGWCC 3 cut(s) 162, 294, 594
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 1013
EgeI GGCGCC 2 cut(s) 238, 740
EheI GGCGCC 2 cut(s) 238, 740
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 4 cut(s) 172, 498, 772, 975
FaqI GGGAC 2 cut(s) 307, 1010
FatI CATG 4 cut(s) 168, 494, 768, 971
FauI CCCGC 2 cut(s) 265, 987
FbaI TGATCA 1 cut(s) 664
FokI GGATG 1 cut(s) 641
FriOI GRGCYC 1 cut(s) 1013
GlaI GCGC 3 cut(s) 238, 456, 740
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 2 cut(s) 240, 742
HaeIII GGCC 3 cut(s) 392, 806, 1028
HapII CCGG 7 cut(s) 108, 160, 306, 345, 381, 422, 449
HgaI GACGC 1 cut(s) 364
HhaI GCGC 3 cut(s) 239, 457, 741
Hin1I GRCGYC 3 cut(s) 237, 375, 739
Hin1II CATG 4 cut(s) 172, 498, 772, 975
Hin6I GCGC 3 cut(s) 237, 455, 739
HinP1I GCGC 3 cut(s) 237, 455, 739
HincII GTYRAC 2 cut(s) 565, 646
HindII GTYRAC 2 cut(s) 565, 646
HinfI GANTC 3 cut(s) 335, 647, 812
HpaII CCGG 7 cut(s) 108, 160, 306, 345, 381, 422, 449
HphI GGTGA 4 cut(s) 20, 47, 768, 1121
Hpy166II GTNNAC 4 cut(s) 229, 565, 646, 766
Hpy188I TCNGA 4 cut(s) 598, 615, 715, 802
Hpy188III TCNNGA 6 cut(s) 108, 210, 345, 422, 689, 1122
Hpy8I GTNNAC 4 cut(s) 229, 565, 646, 766
Hpy99I CGWCG 4 cut(s) 324, 327, 380, 415
HpyAV CCTTC 3 cut(s) 100, 168, 601
HpyCH4III ACNGT 3 cut(s) 63, 226, 269
HpyCH4IV ACGT 3 cut(s) 248, 410, 943
HpyCH4V TGCA 5 cut(s) 507, 766, 866, 887, 1151
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 251, 398, 504, 884, 1034
HpyF3I CTNAG 4 cut(s) 312, 572, 690, 1052
HpySE526I ACGT 3 cut(s) 248, 410, 943
Hsp92I GRCGYC 3 cut(s) 237, 375, 739
Hsp92II CATG 4 cut(s) 172, 498, 772, 975
HspAI GCGC 3 cut(s) 237, 455, 739
KasI GGCGCC 2 cut(s) 236, 738
Kpn2I TCCGGA 3 cut(s) 107, 344, 421
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 4 cut(s) 68, 132, 212, 664
LmnI GCTCC 5 cut(s) 424, 634, 1016, 1093, 1129
Lsp1109I GCAGC 8 cut(s) 373, 439, 649, 688, 710, 871, 899, 974
LweI GCATC 2 cut(s) 970, 1113
MaeII ACGT 3 cut(s) 248, 410, 943
MaeIII GTNAC 2 cut(s) 406, 774
MalI GATC 4 cut(s) 70, 134, 214, 666
MboI GATC 4 cut(s) 68, 132, 212, 664
MboII GAAGA 6 cut(s) 263, 699, 702, 737, 928, 931
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 3 cut(s) 768, 1013, 1098
MluCI AATT 9 cut(s) 11, 23, 369, 660, 681, 841, 861, 911, 1021
Mly113I GGCGCC 2 cut(s) 237, 739
MlyI GAGTC 2 cut(s) 641, 821
MroI TCCGGA 3 cut(s) 107, 344, 421
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 6 cut(s) 84, 516, 588, 671, 987, 1158
MspA1I CMGCKG 3 cut(s) 364, 640, 701
MspI CCGG 7 cut(s) 108, 160, 306, 345, 381, 422, 449
MspR9I CCNGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 6 cut(s) 165, 251, 398, 504, 884, 1034
NarI GGCGCC 2 cut(s) 237, 739
NdeII GATC 4 cut(s) 68, 132, 212, 664
NlaIII CATG 4 cut(s) 172, 498, 772, 975
NlaIV GGNNCC 5 cut(s) 238, 295, 612, 740, 1012
NmuCI GTSAC 2 cut(s) 406, 774
NspI RCATGY 2 cut(s) 498, 772
PaeI GCATGC 1 cut(s) 498
PciI ACATGT 1 cut(s) 768
PctI GAATGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 335
PinAI ACCGGT 1 cut(s) 305
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 2 cut(s) 641, 820
PluTI GGCGCC 2 cut(s) 240, 742
PpsI GAGTC 2 cut(s) 641, 820
PscI ACATGT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 5 cut(s) 238, 295, 612, 740, 1012
PspPI GGNCC 4 cut(s) 162, 294, 594, 805
PstI CTGCAG 1 cut(s) 889
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 3 cut(s) 364, 640, 701
RsaI GTAC 4 cut(s) 290, 488, 896, 1118
RsaNI GTAC 4 cut(s) 289, 487, 895, 1117
SaqAI TTAA 6 cut(s) 84, 516, 588, 671, 987, 1158
Sau3AI GATC 4 cut(s) 68, 132, 212, 664
Sau96I GGNCC 4 cut(s) 162, 294, 594, 805
SchI GAGTC 2 cut(s) 641, 821
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 3 cut(s) 768, 1013, 1098
SfaNI GCATC 2 cut(s) 970, 1113
SfcI CTRYAG 1 cut(s) 885
SfoI GGCGCC 2 cut(s) 238, 740
SinI GGWCC 3 cut(s) 162, 294, 594
SmlI CTYRAG 2 cut(s) 809, 1120
SmoI CTYRAG 2 cut(s) 809, 1120
SphI GCATGC 1 cut(s) 498
Sse9I AATT 9 cut(s) 11, 23, 369, 660, 681, 841, 861, 911, 1021
SsiI CCGC 4 cut(s) 234, 258, 443, 994
SspDI GGCGCC 2 cut(s) 236, 738
SspI AATATT 1 cut(s) 931
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 63, 226, 269
TaiI ACGT 3 cut(s) 251, 413, 946
TaqI TCGA 3 cut(s) 131, 325, 333
TaqII GACCGA 1 cut(s) 385
TasI AATT 9 cut(s) 11, 23, 369, 660, 681, 841, 861, 911, 1021
TatI WGTACW 1 cut(s) 894
TauI GCSGC 2 cut(s) 237, 445
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 6 cut(s) 84, 516, 588, 671, 987, 1158
Tru9I TTAA 6 cut(s) 84, 516, 588, 671, 987, 1158
TscAI CASTG 3 cut(s) 247, 749, 873
TseFI GTSAC 2 cut(s) 406, 774
TseI GCWGC 8 cut(s) 361, 452, 637, 698, 701, 884, 887, 962
Tsp45I GTSAC 2 cut(s) 406, 774
TspDTI ATGAA 4 cut(s) 17, 738, 954, 972
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 3 cut(s) 247, 749, 873
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 162, 294, 594
XapI RAATTY 1 cut(s) 11
XceI RCATGY 2 cut(s) 498, 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.