pycom07g00340

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003709
Physical Location & Seq
Forward (+)
330124 .. 333328
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 852 bp
ATGTTCAATGTATTTGAGCGGCCGAAGAGAACGAGTTGTAGAAACATGTTGGGTGGTAAAGGAGGACGTCTTCTTTTTTGCTTCATCCTCGTGCTTTTTGCTTTGTTAGCAAGTGCTGCTGTGGAAGTTGAACCGAGTCATTATTCGAGGCCATTTAATCGAACTAGCTTCCCACCTGGGTTCATCTTTGGAGCCGGTTTAGCTGCTTATCAGTCTGAAGGAGCAGCTCTTGTTCACGGAAAGGGACCAAGTATATGGGATACCTTCACTAGGGAACATCCAGAAAAAATTTCGGATGGTAGCAATGGGAATGCAGCCAATGATTTTTACAATCACTATAAGGAGGACATAAAATTGATAAAAAAGATCGGCTTGGACTCCTTCAGATTTTCTTTCTCATGGTCAAGAATACTACCTAAGGGTACAATTAAAGGTGGAGTGAACCAAGAAGGAGTCAACTTCTACAACAATCTGATAGATGAGCTCTTATCCAATGGAATTGTGCCTTTTGTGACCCTTTTCCATTGGGATACACCACAAGCACTTGAAGATGAGTACGGTGGATTTTTAAGCCCTAAGATTGTGAATGATTTTCAAGATTATGCCAATTTTTGTTTCAAAACATTTGGGGATCGTGTTAAGCATTGGGTGACATTGAACGAGCCAATTACATATTGTGTTAATGGATACAATGGCGGTACTTATGCACCCGGTCGATGTTCAAACTATGTCGGAAATTGCACTGCTGGTAACTCTGCCACTGAGCCTTACATTGTTGGTCATCATCTACTACTTGCTCATGCACACGCTGTGAAGATATACAGGCACAAGTATCAGAACACTGTGGAGTAA

Protein Analysis

284

Amino Acids

31.66

Weight (kDa)

8.15

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 55 - 281 3.1e-86 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
AccBSI CCGCTC 1 cut(s) 19
AciI CCGC 2 cut(s) 19, 696
AclWI GGATC 1 cut(s) 639
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 2 cut(s) 237, 367
AcyI GRCGYC 1 cut(s) 67
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 3 cut(s) 424, 557, 700
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 7 cut(s) 7, 131, 548, 596, 619, 658, 723
AjnI CCWGG 1 cut(s) 175
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AluBI AGCT 4 cut(s) 168, 203, 227, 484
AluI AGCT 4 cut(s) 168, 203, 227, 484
Alw21I GWGCWC 1 cut(s) 486
AlwI GGATC 1 cut(s) 639
AoxI GGCC 2 cut(s) 20, 149
ApeKI GCWGC 4 cut(s) 116, 203, 224, 314
ApoI RAATTY 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 711
AsuHPI GGTGA 1 cut(s) 661
AvaII GGWCC 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 417
BanII GRGCYC 1 cut(s) 486
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 4 cut(s) 103, 190, 236, 326
BccI CCATC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 177
BciVI GTATCC 3 cut(s) 253, 523, 680
BcnI CCSGG 1 cut(s) 711
BfaI CTAG 2 cut(s) 165, 270
BfuI GTATCC 3 cut(s) 253, 523, 680
BisI GCNGC 5 cut(s) 20, 117, 204, 225, 315
BlsI GCNGC 5 cut(s) 21, 118, 205, 226, 316
Bme1390I CCNGG 2 cut(s) 177, 711
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 193, 246
BmrFI CCNGG 2 cut(s) 177, 711
BpiI GAAGAC 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 194
Bse21I CCTNAGG 1 cut(s) 417
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 3 cut(s) 84, 277, 301
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 753
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 103, 190, 236, 326
Bsh1285I CGRYCG 2 cut(s) 23, 715
BshFI GGCC 2 cut(s) 22, 151
BsiEI CGRYCG 2 cut(s) 23, 715
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 2 cut(s) 195, 711
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 2 cut(s) 22, 151
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 2 cut(s) 366, 631
BspACI CCGC 2 cut(s) 19, 696
BspANI GGCC 2 cut(s) 22, 151
BspCNI CTCAG 1 cut(s) 754
BspLI GGNNCC 2 cut(s) 193, 246
BspPI GGATC 1 cut(s) 639
BsrBI CCGCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 2 cut(s) 366, 631
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 89
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 560, 844
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 116
BstDEI CTNAG 3 cut(s) 417, 576, 762
BstENI CCTNNNNNAGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 84, 277, 301
BstKTI GATC 2 cut(s) 369, 634
BstMBI GATC 2 cut(s) 366, 631
BstMCI CGRYCG 2 cut(s) 23, 715
BstMWI GCNNNNNNNGC 3 cut(s) 107, 116, 200
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 175, 709
BstV1I GCAGC 4 cut(s) 103, 190, 236, 326
BstV2I GAAGAC 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 20
Bsu36I CCTNAGG 1 cut(s) 417
BsuI GTATCC 3 cut(s) 253, 523, 680
BsuRI GGCC 2 cut(s) 22, 151
BtsCI GGATG 3 cut(s) 84, 277, 301
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 3 cut(s) 741, 759, 840
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 423, 556, 699
CviAII CATG 3 cut(s) 46, 399, 800
CviQI GTAC 3 cut(s) 423, 556, 699
DdeI CTNAG 3 cut(s) 417, 576, 762
DpnI GATC 2 cut(s) 368, 633
DpnII GATC 2 cut(s) 366, 631
DraIII CACNNNGTG 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 20
EagI CGGCCG 1 cut(s) 20
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 484
EclXI CGGCCG 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 2 cut(s) 237, 367
Eco81I CCTNAGG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 484
EcoNI CCTNNNNNAGG 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 486
FaeI CATG 3 cut(s) 49, 402, 803
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 258
FatI CATG 3 cut(s) 45, 398, 799
Fnu4HI GCNGC 5 cut(s) 20, 117, 204, 225, 315
FokI GGATG 3 cut(s) 71, 264, 308
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 5 cut(s) 20, 117, 204, 225, 315
FspBI CTAG 2 cut(s) 165, 270
GluI GCNGC 5 cut(s) 20, 117, 204, 225, 315
HaeIII GGCC 2 cut(s) 22, 151
HapII CCGG 2 cut(s) 195, 711
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 3 cut(s) 49, 402, 803
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 3 cut(s) 136, 377, 453
HpaII CCGG 2 cut(s) 195, 711
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 3 cut(s) 235, 442, 457
Hpy188I TCNGA 6 cut(s) 217, 295, 386, 474, 734, 837
Hpy188III TCNNGA 3 cut(s) 281, 405, 596
Hpy8I GTNNAC 3 cut(s) 235, 442, 457
HpyAV CCTTC 4 cut(s) 212, 274, 391, 443
HpyCH4III ACNGT 2 cut(s) 560, 844
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 4 cut(s) 314, 707, 741, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 116, 200
HpyF3I CTNAG 3 cut(s) 417, 576, 762
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 3 cut(s) 49, 402, 803
Kzo9I GATC 2 cut(s) 366, 631
LmnI GCTCC 2 cut(s) 191, 221
LpnPI CCDG 7 cut(s) 162, 189, 208, 294, 724, 732, 808
Lsp1109I GCAGC 4 cut(s) 103, 190, 236, 326
MaeI CTAG 2 cut(s) 165, 270
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 3 cut(s) 511, 649, 749
MalI GATC 2 cut(s) 368, 633
MbiI CCGCTC 1 cut(s) 19
MboI GATC 2 cut(s) 366, 631
MboII GAAGA 4 cut(s) 37, 62, 560, 826
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 7 cut(s) 288, 353, 426, 498, 607, 666, 736
MlyI GAGTC 3 cut(s) 145, 371, 462
MmeI TCCRAC 1 cut(s) 712
MnlI CCTC 4 cut(s) 56, 98, 141, 337
MseI TTAA 5 cut(s) 156, 429, 569, 639, 681
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 2 cut(s) 195, 711
MspR9I CCNGG 2 cut(s) 177, 711
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 107, 116, 200
NciI CCSGG 1 cut(s) 711
NdeII GATC 2 cut(s) 366, 631
NlaIII CATG 3 cut(s) 49, 402, 803
NlaIV GGNNCC 2 cut(s) 193, 246
NmuCI GTSAC 2 cut(s) 511, 649
NspI RCATGY 1 cut(s) 49
PciI ACATGT 1 cut(s) 45
PctI GAATGC 1 cut(s) 316
PkrI GCNGC 5 cut(s) 21, 118, 205, 226, 316
PleI GAGTC 3 cut(s) 144, 371, 461
PpsI GAGTC 3 cut(s) 144, 371, 461
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 193, 246
PspPI GGNCC 1 cut(s) 245
RsaI GTAC 3 cut(s) 424, 557, 700
RsaNI GTAC 3 cut(s) 423, 556, 699
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 5 cut(s) 156, 429, 569, 639, 681
SatI GCNGC 5 cut(s) 20, 117, 204, 225, 315
Sau3AI GATC 2 cut(s) 366, 631
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 3 cut(s) 145, 371, 462
ScrFI CCNGG 2 cut(s) 177, 711
SduI GDGCHC 1 cut(s) 486
SetI ASST 9 cut(s) 70, 170, 178, 205, 229, 266, 418, 436, 486
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 7 cut(s) 288, 353, 426, 498, 607, 666, 736
SsiI CCGC 2 cut(s) 19, 696
SspMI CTAG 2 cut(s) 165, 270
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 175, 709
TaaI ACNGT 2 cut(s) 560, 844
TaiI ACGT 1 cut(s) 70
TaqI TCGA 3 cut(s) 146, 160, 715
TasI AATT 7 cut(s) 288, 353, 426, 498, 607, 666, 736
TauI GCSGC 1 cut(s) 22
Tru1I TTAA 5 cut(s) 156, 429, 569, 639, 681
Tru9I TTAA 5 cut(s) 156, 429, 569, 639, 681
TscAI CASTG 3 cut(s) 748, 766, 847
TseFI GTSAC 2 cut(s) 511, 649
TseI GCWGC 4 cut(s) 116, 203, 224, 314
Tsp45I GTSAC 2 cut(s) 511, 649
TspDTI ATGAA 2 cut(s) 73, 172
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 3 cut(s) 748, 766, 847
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 268
XapI RAATTY 1 cut(s) 288
XceI RCATGY 1 cut(s) 49
XspI CTAG 2 cut(s) 165, 270
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.