pycom07g00460

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005851
Physical Location & Seq
Reverse (-)
440391 .. 451224
10834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3492 bp
ATGGATATAAGACAATTTCTGCTTGATGCGCCAGAGACATGTTTCTTCACATGTTATGACTTATTGCTACACACAAAGGATGGCCCCACTCATCATTTGGAAGACTTTAATGAAATTTCCGAAGTTGCTGATATTACCGTTGGTGGTTGCTCCTTGGAGATGGTTCCTGCTTTATATGATGATAGATCTATAAGGGCTCATGTTCATCGTACGAGAGAATTGCTTTCCCTTTCTACACTTCATGCATCACTATCGACGTCACTTGCATTACAGTATGAGACAGCACAAAACAAAGTGGCGAGCCCTGGAGATACTGTGAAAACTGAGGTCCCAGGGCTTGATGGTTTGGGTTTTATGGAGGATGTTGCTGGTTCATTGAGCAATTTGCTATCTTCACCCTCAAAAGAGATAAAATGTGTGGAGAGTATTGTGTTTTCATCCTTCAATCCTCCGCCAAGCTATAGAAGGCTTGTTGGGGATTTGATTTATTTGGATATAGTAACATTGGAGGGCAATAAACACTGTATTACTGGGACTACAAAAATGTTTTATGTCAATTCCAGCACGGGGAACACTCTTGACCCTAAGCCAAGTAAAAGTAATTGGGAAGCAACCACACTTGTTGGACTTTTGCAAAATGTTAGCTCTAAGTTCAAAAAAGCTTTTCGTGAAATTTTGGAGCGGAGGGCATCTGCACATCCTTTTGAAAATGTTCAGTCTTTGTTGCCACCAAATTCATGGCTAGGGTTATATCCAGTTCCAGATCACAAGCGTGATGCAGCTAGAGCAGAGGATGCCCTTACTCTTTCATACGGCAGTGAGCTAATTGGCATGCAAAGGGATTGGAATGAGGAGTTGCAATCTTGTAGGGAATTTCCACATACAACTCCTCAGGAAAGAATTTTGCGGGATAGGGCTCTATACAAAGTGACTTCAGACTTTGTTGATGCAGCTATTAGTGGTGCTATTGGAGTTATTAGTAGATGCATACCTCCTATAAACCCAACTGATCCAGAGTGCTTCCACATGTATGTTCACAATAATATATTCTTCAGTTTTGCGGTTGATGCGGACCTTGAGCAGCTGTCTAAGAAACATGCGTCAGATTCAAGTCCAAAGATAGGGGGCACTGGTTCTGTACACAGTTCTTCTGAGAAGGCTACTGACAATTTGTTGCATGGAGAGAGTGCAATTCCCAATAGAGAAAAATGCAAAGGATCAAGTACAATCGATGATGCGACAGAATCTTCCCCTGATGTTTCTGCCGAGACGCAATTGGGTGAAACCGAGCAGGCTACTTATGCTTCTGCAAATAATGATTTGAAAGGCACCAAGGCGTACCAGGAAGCTGATGTCTCTGGACTTTATAATCTTGCTATGGCCATAATCGATTACAGGGGGCATAGAGTAGTAGCTCAGAGTGTTTTACCGGGCATTCTTCAAGGGGATAAATCAGACTCTCTTCTATATGGTTCTGTTGATAATGGCAAGAAAATATGCTGGAATGAAGAGTTCCATTCCAAGGTGCTGGAAGCTGCTAAGCGCCTTCATCTTAAAGAGCACACAGTCCTTGATGGATCTGGAAATGTTTTCAGATTGGCTGCACCGGTGGAATGCAAGGGTATTGTTGGTAGTGATGACAGGCATTATCTCCTCGACTTGATGAGAGTGACTCCTCGTGATTCAAACTGCACTGGACCTGGGTCCAGATTTTGTATCTTGAGGCTGGAATTAATTACTGCCTATTGCCAGGCCCAAGCTGCAGAGAAACCAAAATCTAAGTCTAAAGATGGAGAAGGTCTTGTCACCACTGATTCTTCAGTCATCACTGATGCTAAGCAGGCCATAACGGAAGAAGGGAATGCCACAGACGCACAAGAAATTGCATCTCCACCTCCATCCACTGAAAGCTCTGATCCATGTGAAGAAATTCTTTTTAACCCTAATGTCTTCACCGAGTTTAAGTTGGCTGGAAATGAGGAGGAGATAGCAGAAGATGAAGGAAATGTGAGGAAAGCCAGTTTGTATCTTACAGATGTTGTGCTTCCAAAATTTATACAAGACCTCTGCACGCTTGAAGTATCACCCATGGACGGGCAGACGCTAACTGAAGCACTCCATGCTCATGGAATTAATGTTCGCTATATTGGAAAAGTGGCTGAGGGGACCAGACATTTACCTCATCTTTGGGATCTTTGCTCCAATGAGATAGTGGTTAGATCTGCTAAGCACATTCTCAAGGATGCCTTGAGAGAAACGAATGATCATGATATTGGGCCCGCAATCACCCATTTCTTCAACTGTTTCTTTGGAAGCTGTCAAGCCGTTGGCTCAAAAGTTGCTGCTAATAATATGCAGTCTAGAACGCCCAAAAGGGAACAAACAGGACAGCAATCTCCAAGAAAATCGTCTAAGGGACTAGGAAAATTGAAGGATGGAGTATCTGCTAGAAAGAGTCGATCCTCATTTATGCTTGCTAGCTCAGAAACTCTATGGTCCGACATTCAAGAATTTGCAAAACTCAAATATCAGTTTGAGCTGCCGGAGGATGCTAGGATGCGTGTAAAGAAAGATTCTGTTATACGTAATCTTTGCCAAAAGGTGGGCATTACAATTGCAGCTCGCAGATATGATCTTAATTCTGCAGCACCTTTCCAAATGTCAGATATCTTAAATCTTCAACCTGTAGTGAAACATTCAGTTCCTGTCTGTTCAGAAGCTAAGGATCTTGTGGAAACAGGAAAGATCCAACTGGCCGAGGGAATGTTAAGTGAAGCGTACACATTATTTACCGAGGCATTTTCAATACTTCAGCAGGTTACTGGTCCAATGCATAGGGAGGTTGCTAACTGCTGTCGGTATCTTGCCATGGTTCTGTATCATGCGGGAGACATGGCTGGAGCCATAATGCAACAGCATAAGGAACTAATTATAAATGAACGCTGCCTTGGCCTAGACCACCCTGACACTGCTCACAGCTATGGAAATATGGCTCTTTTCTACCATGGACTTAACCAGACAGAACTTGCACTATGGCACATGTCAAGGGCATTGCTTCTGCTAAGTTTGTCATCGGGCCCAGATCAACCTGATGTTGCTGCAACTTTCATTAATGTTGCTATGATGTACCAGATCGATCTTGGGAAGATGGACACTGCTCTTCGATATTTGCAAGAGGCTCTGAAAAAAAATGAAAGGCTTCTAGGGGAGGAGCACATCCAGACTGCTGTATGCTATCACGCACTTGCTATTGCATTTAATTGTATGGGTGCCTTCAAGCTCTCTCACCAGCATGAGAAGAAAACTTATGACATACTAGTCAAACAATTGGGCGAGGAGGATTCTAGAACACGTGACTCTCAAAACTGGATGAAGACATTTAAGATGCGCGAGCTACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTTTGAATGCTGCTTCGGCTCAAAAGGCAATTGACATCTTGAAGGTCTGGCCCATCCTGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N DUF727 TPR_10 TPR_12 eIF3_p135

Protein Analysis

1164

Amino Acids

128.63

Weight (kDa)

5.77

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 2 - 74 5.8e-13 Mitochondrial function, CLU-N-term
CLU PF13236 281 - 374 4.4e-26 Clustered mitochondria
CLU PF13236 424 - 563 9.9e-46 Clustered mitochondria
eIF3_p135 PF12807 694 - 878 2e-47 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 955 - 1018 2.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 1004 - 1133 1.9e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 1031 - 1105 2.7e-12 Tetratricopeptide repeat
TPR_10 PF13374 1032 - 1073 2.3e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1368, 2933
AasI GACNNNNNNGTC 1 cut(s) 3317
AatII GACGTC 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 2806
AccB1I GGYRCC 2 cut(s) 1328, 3269
AccB7I CCANNNNNTGG 1 cut(s) 2602
AccBSI CCGCTC 1 cut(s) 682
AccII CGCG 1 cut(s) 3390
AciI CCGC 7 cut(s) 452, 682, 907, 1061, 1070, 2280, 2885
AclWI GGATC 8 cut(s) 1004, 1225, 1585, 1910, 2199, 2454, 2733, 2740
AcoI YGGCCR 2 cut(s) 1380, 2754
AcsI RAATTY 8 cut(s) 114, 672, 733, 872, 900, 1929, 2051, 2510
AcuI CTGAAG 5 cut(s) 918, 1036, 1803, 2130, 2795
AcvI CACGTG 1 cut(s) 3353
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 6 cut(s) 211, 1140, 1225, 1340, 2780, 3128
AfiI CCNNNNNNNGG 6 cut(s) 567, 1121, 1522, 2093, 2094, 2602
AflIII ACRYGT 5 cut(s) 38, 50, 1026, 3039, 3350
AgeI ACCGGT 1 cut(s) 1606
AhlI ACTAGT 1 cut(s) 3316
AjnI CCWGG 5 cut(s) 304, 331, 1341, 1699, 1749
AjuI GAANNNNNNNTTGG 2 cut(s) 2931, 2963
AleI CACNNNNGTG 1 cut(s) 771
AloI GAACNNNNNNTCC 2 cut(s) 2121, 2153
Alw21I GWGCWC 2 cut(s) 1563, 3216
Alw26I GTCTC 5 cut(s) 29, 272, 1262, 1360, 2883
AlwI GGATC 8 cut(s) 1004, 1225, 1585, 1910, 2199, 2454, 2733, 2740
AlwNI CAGNNNCTG 1 cut(s) 2705
AoxI GGCC 9 cut(s) 82, 1380, 1752, 1842, 2276, 2754, 2950, 3076, 3476
ApaI GGGCCC 2 cut(s) 2280, 3080
ApoI RAATTY 8 cut(s) 114, 672, 733, 872, 900, 1929, 2051, 2510
ArsI GACNNNNNNTTYG 2 cut(s) 1766, 1798
AseI ATTAAT 3 cut(s) 1733, 2133, 3111
AsiGI ACCGGT 1 cut(s) 1606
Asp700I GAANNNNTTC 3 cut(s) 711, 1929, 3198
AspLEI GCGC 3 cut(s) 31, 1545, 3390
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 7 cut(s) 387, 1292, 1798, 1945, 2076, 2278, 3278
AsuNHI GCTAGC 1 cut(s) 2477
AvaII GGWCC 7 cut(s) 328, 1072, 1697, 1704, 2166, 2496, 2825
AxyI CCTNAGG 1 cut(s) 891
BaeGI GKGCMC 3 cut(s) 1130, 2280, 3080
BalI TGGCCA 1 cut(s) 1382
BanI GGYRCC 2 cut(s) 1328, 3269
BanII GRGCYC 5 cut(s) 199, 305, 919, 2280, 3080
BauI CACGAG 1 cut(s) 1677
BbrPI CACGTG 1 cut(s) 3353
BbsI GAAGAC 3 cut(s) 108, 1942, 3380
Bbv12I GWGCWC 2 cut(s) 1563, 3216
BbvCI CCTCAGC 1 cut(s) 2160
BccI CCATC 9 cut(s) 74, 154, 335, 1568, 1784, 1906, 2429, 3142, 3488
BceAI ACGGC 2 cut(s) 829, 2309
BcgI CGANNNNNNTGC 4 cut(s) 202, 234, 236, 268
BciT130I CCWGG 5 cut(s) 306, 333, 1343, 1701, 1751
BclI TGATCA 1 cut(s) 2263
BcnI CCSGG 1 cut(s) 1431
BcoDI GTCTC 5 cut(s) 29, 272, 1262, 1360, 2883
BcuI ACTAGT 1 cut(s) 3316
BfmI CTRYAG 5 cut(s) 460, 1761, 2643, 2685, 3395
BfoI RGCGCY 1 cut(s) 1546
BfuAI ACCTGC 1 cut(s) 2806
BglII AGATCT 2 cut(s) 185, 2219
BlpI GCTNAGC 3 cut(s) 1539, 1836, 2226
Bme1390I CCNGG 6 cut(s) 306, 333, 1343, 1431, 1701, 1751
Bme18I GGWCC 7 cut(s) 328, 1072, 1697, 1704, 2166, 2496, 2825
BmrFI CCNGG 6 cut(s) 306, 333, 1343, 1431, 1701, 1751
BmrI ACTGGG 1 cut(s) 540
BmtI GCTAGC 1 cut(s) 2481
BmuI ACTGGG 1 cut(s) 540
BoxI GACNNNNGTC 1 cut(s) 1702
BpiI GAAGAC 3 cut(s) 108, 1942, 3380
BplI GAGNNNNNCTC 2 cut(s) 1657, 1689
BpmI CTGGAG 2 cut(s) 327, 2919
Bpu10I CCTNAGC 3 cut(s) 585, 2160, 2721
Bpu1102I GCTNAGC 3 cut(s) 1539, 1836, 2226
BpuEI CTTGAG 4 cut(s) 1097, 1741, 2222, 2269
BpuMI CCSGG 1 cut(s) 1431
Bsa29I ATCGAT 3 cut(s) 1230, 1389, 3135
BsaAI YACGTR 2 cut(s) 2585, 3353
BsaHI GRCGYC 1 cut(s) 257
BsaWI WCCGGW 1 cut(s) 1606
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 6 cut(s) 567, 1121, 1522, 2093, 2094, 2602
Bse118I RCCGGY 1 cut(s) 1606
Bse1I ACTGG 8 cut(s) 535, 755, 1135, 1699, 2019, 2757, 2827, 3371
Bse21I CCTNAGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 3050
BseBI CCWGG 5 cut(s) 306, 333, 1343, 1701, 1751
BseCI ATCGAT 3 cut(s) 1230, 1389, 3135
BseLI CCNNNNNNNGG 6 cut(s) 567, 1121, 1522, 2093, 2094, 2602
BseMI GCAATG 1 cut(s) 3050
BseMII CTCAG 6 cut(s) 315, 905, 1143, 1430, 2151, 2496
BseNI ACTGG 8 cut(s) 535, 755, 1135, 1699, 2019, 2757, 2827, 3371
BseRI GAGGAG 8 cut(s) 866, 879, 1643, 1665, 1994, 1997, 3224, 3350
BseSI GKGCMC 3 cut(s) 1130, 2280, 3080
BsgI GTGCAG 4 cut(s) 678, 1587, 1675, 2053
Bsh1236I CGCG 1 cut(s) 3390
BshFI GGCC 9 cut(s) 84, 1382, 1754, 1844, 2278, 2756, 2952, 3078, 3478
BshNI GGYRCC 2 cut(s) 1328, 3269
BshTI ACCGGT 1 cut(s) 1606
BshVI ATCGAT 3 cut(s) 1230, 1389, 3135
BsiHKAI GWGCWC 2 cut(s) 1563, 3216
BsiSI CCGG 3 cut(s) 1430, 1607, 2543
BsiWI CGTACG 1 cut(s) 209
BslFI GGGAC 4 cut(s) 314, 547, 2179, 2430
BslI CCNNNNNNNGG 6 cut(s) 567, 1121, 1522, 2093, 2094, 2602
BsmAI GTCTC 5 cut(s) 29, 272, 1262, 1360, 2883
BsmBI CGTCTC 1 cut(s) 1262
BsmFI GGGAC 4 cut(s) 314, 547, 2179, 2430
BsmI GAATGC 5 cut(s) 1434, 1619, 1867, 3409, 3439
BsnI GGCC 9 cut(s) 84, 1382, 1754, 1844, 2278, 2756, 2952, 3078, 3478
Bsp120I GGGCCC 2 cut(s) 2276, 3076
Bsp1286I GDGCHC 8 cut(s) 199, 305, 919, 1130, 1563, 2280, 3080, 3216
Bsp1407I TGTACA 1 cut(s) 1138
Bsp1720I GCTNAGC 3 cut(s) 1539, 1836, 2226
Bsp19I CCATGG 3 cut(s) 2088, 2868, 3004
BspACI CCGC 7 cut(s) 452, 682, 907, 1061, 1070, 2280, 2885
BspANI GGCC 9 cut(s) 84, 1382, 1754, 1844, 2278, 2756, 2952, 3078, 3478
BspCNI CTCAG 6 cut(s) 316, 904, 1144, 1429, 2152, 2495
BspDI ATCGAT 3 cut(s) 1230, 1389, 3135
BspFNI CGCG 1 cut(s) 3390
BspHI TCATGA 1 cut(s) 2266
BspMAI CTGCAG 2 cut(s) 1765, 2647
BspMI ACCTGC 1 cut(s) 2806
BspOI GCTAGC 1 cut(s) 2481
BspPI GGATC 8 cut(s) 1004, 1225, 1585, 1910, 2199, 2454, 2733, 2740
BspQI GCTCTTC 1 cut(s) 3165
BspT107I GGYRCC 2 cut(s) 1328, 3269
BsrBI CCGCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 3050
BsrFI RCCGGY 1 cut(s) 1606
BsrGI TGTACA 1 cut(s) 1138
BsrI ACTGG 8 cut(s) 535, 755, 1135, 1699, 2019, 2757, 2827, 3371
BssAI RCCGGY 1 cut(s) 1606
BssNI GRCGYC 1 cut(s) 257
BssSI CACGAG 1 cut(s) 1677
BssT1I CCWWGG 7 cut(s) 153, 1332, 1521, 2088, 2868, 2947, 3004
Bst2BI CACGAG 1 cut(s) 1677
Bst2UI CCWGG 5 cut(s) 306, 333, 1343, 1701, 1751
Bst4CI ACNGT 7 cut(s) 139, 273, 316, 524, 1145, 1567, 2303
Bst6I CTCTTC 3 cut(s) 1467, 1503, 3165
BstACI GRCGYC 1 cut(s) 257
BstAPI GCANNNNNTGC 2 cut(s) 794, 2120
BstAUI TGTACA 1 cut(s) 1138
BstBAI YACGTR 2 cut(s) 2585, 3353
BstDSI CCRYGG 3 cut(s) 2088, 2868, 3004
BstFNI CGCG 1 cut(s) 3390
BstH2I RGCGCY 1 cut(s) 1546
BstHHI GCGC 3 cut(s) 31, 1545, 3390
BstMAI GTCTC 5 cut(s) 29, 272, 1262, 1360, 2883
BstNI CCWGG 5 cut(s) 306, 333, 1343, 1701, 1751
BstNSI RCATGY 6 cut(s) 42, 54, 835, 1030, 1100, 3043
BstPAI GACNNNNGTC 1 cut(s) 1702
BstSCI CCNGG 6 cut(s) 304, 331, 1341, 1429, 1699, 1749
BstSFI CTRYAG 5 cut(s) 460, 1761, 2643, 2685, 3395
BstSLI GKGCMC 3 cut(s) 1130, 2280, 3080
BstSNI TACGTA 1 cut(s) 2585
BstUI CGCG 1 cut(s) 3390
BstV2I GAAGAC 3 cut(s) 108, 1942, 3380
BstX2I RGATCY 6 cut(s) 185, 1577, 2191, 2219, 2725, 2745
BstXI CCANNNNNNTGG 3 cut(s) 738, 1528, 2126
BstYI RGATCY 6 cut(s) 185, 1577, 2191, 2219, 2725, 2745
Bsu15I ATCGAT 3 cut(s) 1230, 1389, 3135
Bsu36I CCTNAGG 1 cut(s) 891
BsuRI GGCC 9 cut(s) 84, 1382, 1754, 1844, 2278, 2756, 2952, 3078, 3478
BsuTUI ATCGAT 3 cut(s) 1230, 1389, 3135
BtgI CCRYGG 3 cut(s) 2088, 2868, 3004
BtsI GCAGTG 3 cut(s) 823, 2967, 3153
BtsIMutI CAGTG 9 cut(s) 520, 823, 1128, 1692, 1809, 1827, 1902, 2967, 3153
BveI ACCTGC 1 cut(s) 2806
CaiI CAGNNNCTG 1 cut(s) 2705
CciI TCATGA 1 cut(s) 2266
CfoI GCGC 3 cut(s) 31, 1545, 3390
Cfr10I RCCGGY 1 cut(s) 1606
ClaI ATCGAT 3 cut(s) 1230, 1389, 3135
CseI GACGC 4 cut(s) 1089, 1279, 1880, 2110
Csp6I GTAC 6 cut(s) 210, 1139, 1224, 1339, 2779, 3127
CspAI ACCGGT 1 cut(s) 1606
CspCI CAANNNNNGTGG 2 cut(s) 604, 639
CviQI GTAC 6 cut(s) 210, 1139, 1224, 1339, 2779, 3127
DrdI GACNNNNNNGTC 1 cut(s) 3317
DseDI GACNNNNNNGTC 1 cut(s) 3317
EaeI YGGCCR 2 cut(s) 1380, 2754
Eam1104I CTCTTC 3 cut(s) 1467, 1503, 3165
EarI CTCTTC 3 cut(s) 1467, 1503, 3165
EciI GGCGGA 1 cut(s) 441
Eco105I TACGTA 1 cut(s) 2585
Eco130I CCWWGG 7 cut(s) 153, 1332, 1521, 2088, 2868, 2947, 3004
Eco24I GRGCYC 5 cut(s) 199, 305, 919, 2280, 3080
Eco32I GATATC 1 cut(s) 2668
Eco47I GGWCC 7 cut(s) 328, 1072, 1697, 1704, 2166, 2496, 2825
Eco57I CTGAAG 5 cut(s) 918, 1036, 1803, 2130, 2795
Eco72I CACGTG 1 cut(s) 3353
Eco81I CCTNAGG 1 cut(s) 891
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 5 cut(s) 304, 331, 1341, 1699, 1749
EcoRV GATATC 1 cut(s) 2668
EcoT14I CCWWGG 7 cut(s) 153, 1332, 1521, 2088, 2868, 2947, 3004
EcoT22I ATGCAT 3 cut(s) 247, 989, 2835
EcoT38I GRGCYC 5 cut(s) 199, 305, 919, 2280, 3080
ErhI CCWWGG 7 cut(s) 153, 1332, 1521, 2088, 2868, 2947, 3004
Esp3I CGTCTC 1 cut(s) 1262
FalI AAGNNNNNCTT 4 cut(s) 1917, 1949, 2231, 2263
FaqI GGGAC 4 cut(s) 314, 547, 2179, 2430
FauI CCCGC 3 cut(s) 900, 2287, 2878
FbaI TGATCA 1 cut(s) 2263
FriOI GRGCYC 5 cut(s) 199, 305, 919, 2280, 3080
GlaI GCGC 3 cut(s) 30, 1544, 3389
GsuI CTGGAG 2 cut(s) 327, 2919
HaeII RGCGCY 1 cut(s) 1546
HaeIII GGCC 9 cut(s) 84, 1382, 1754, 1844, 2278, 2756, 2952, 3078, 3478
HapII CCGG 3 cut(s) 1430, 1607, 2543
HgaI GACGC 4 cut(s) 1089, 1279, 1880, 2110
HhaI GCGC 3 cut(s) 31, 1545, 3390
Hin1I GRCGYC 1 cut(s) 257
Hin6I GCGC 3 cut(s) 29, 1543, 3388
HinP1I GCGC 3 cut(s) 29, 1543, 3388
HindIII AAGCTT 2 cut(s) 660, 3426
HpaII CCGG 3 cut(s) 1430, 1607, 2543
HphI GGTGA 7 cut(s) 387, 1292, 1798, 1945, 2076, 2278, 3278
Hpy166II GTNNAC 3 cut(s) 1036, 1141, 2781
Hpy8I GTNNAC 3 cut(s) 1036, 1141, 2781
Hpy99I CGWCG 1 cut(s) 259
HpyCH4III ACNGT 7 cut(s) 139, 273, 316, 524, 1145, 1567, 2303
HpyCH4IV ACGT 3 cut(s) 257, 2584, 3352
HpySE526I ACGT 3 cut(s) 257, 2584, 3352
Hsp92I GRCGYC 1 cut(s) 257
HspAI GCGC 3 cut(s) 29, 1543, 3388
Ksp22I TGATCA 1 cut(s) 2263
LguI GCTCTTC 1 cut(s) 3165
LmnI GCTCC 5 cut(s) 155, 679, 2204, 2900, 3211
MaeII ACGT 3 cut(s) 257, 2584, 3352
MaeIII GTNAC 7 cut(s) 258, 499, 928, 1669, 1804, 2818, 3353
MbiI CCGCTC 1 cut(s) 682
MfeI CAATTG 4 cut(s) 1274, 2613, 3326, 3456
MflI RGATCY 6 cut(s) 185, 1577, 2191, 2219, 2725, 2745
MhlI GDGCHC 8 cut(s) 199, 305, 919, 1130, 1563, 2280, 3080, 3216
MlsI TGGCCA 1 cut(s) 1382
MluNI TGGCCA 1 cut(s) 1382
MlyI GAGTC 4 cut(s) 1451, 1666, 2464, 3350
MmeI TCCRAC 3 cut(s) 604, 2523, 2773
Mox20I TGGCCA 1 cut(s) 1382
Mph1103I ATGCAT 3 cut(s) 247, 989, 2835
MroXI GAANNNNTTC 3 cut(s) 711, 1929, 3198
MscI TGGCCA 1 cut(s) 1382
MslI CAYNNNNRTG 5 cut(s) 771, 1029, 2124, 2979, 3291
Msp20I TGGCCA 1 cut(s) 1382
MspA1I CMGCKG 1 cut(s) 1084
MspI CCGG 3 cut(s) 1430, 1607, 2543
MspR9I CCNGG 6 cut(s) 306, 333, 1343, 1431, 1701, 1751
MunI CAATTG 4 cut(s) 1274, 2613, 3326, 3456
Mva1269I GAATGC 5 cut(s) 1434, 1619, 1867, 3409, 3439
MvaI CCWGG 5 cut(s) 306, 333, 1343, 1701, 1751
MvnI CGCG 1 cut(s) 3390
NciI CCSGG 1 cut(s) 1431
NcoI CCATGG 3 cut(s) 2088, 2868, 3004
NheI GCTAGC 1 cut(s) 2477
NmeAIII GCCGAG 2 cut(s) 1291, 2782
NmuCI GTSAC 5 cut(s) 258, 928, 1669, 1804, 3353
NsiI ATGCAT 3 cut(s) 247, 989, 2835
NspI RCATGY 6 cut(s) 42, 54, 835, 1030, 1100, 3043
OliI CACNNNNGTG 1 cut(s) 771
PaeI GCATGC 1 cut(s) 835
PagI TCATGA 1 cut(s) 2266
PasI CCCWGGG 1 cut(s) 332
PciI ACATGT 4 cut(s) 38, 50, 1026, 3039
PciSI GCTCTTC 1 cut(s) 3165
PctI GAATGC 5 cut(s) 1434, 1619, 1867, 3409, 3439
PdmI GAANNNNTTC 3 cut(s) 711, 1929, 3198
PfeI GAWTC 6 cut(s) 1106, 1244, 1682, 1814, 2573, 3341
Pfl23II CGTACG 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 2602
PinAI ACCGGT 1 cut(s) 1606
PleI GAGTC 4 cut(s) 1451, 1666, 2463, 3350
PmaCI CACGTG 1 cut(s) 3353
PmlI CACGTG 1 cut(s) 3353
PpsI GAGTC 4 cut(s) 1451, 1666, 2463, 3350
Ppu21I YACGTR 2 cut(s) 2585, 3353
PpuMI RGGWCCY 1 cut(s) 328
PscI ACATGT 4 cut(s) 38, 50, 1026, 3039
PshAI GACNNNNGTC 1 cut(s) 1702
PshBI ATTAAT 3 cut(s) 1733, 2133, 3111
PsiI TTATAA 2 cut(s) 1368, 2933
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 5 cut(s) 304, 331, 1341, 1699, 1749
PspCI CACGTG 1 cut(s) 3353
PspGI CCWGG 5 cut(s) 304, 331, 1341, 1699, 1749
PspLI CGTACG 1 cut(s) 209
PspOMI GGGCCC 2 cut(s) 2276, 3076
PspPPI RGGWCCY 1 cut(s) 328
PstI CTGCAG 2 cut(s) 1765, 2647
PstNI CAGNNNCTG 1 cut(s) 2705
PsuI RGATCY 6 cut(s) 185, 1577, 2191, 2219, 2725, 2745
PvuII CAGCTG 1 cut(s) 1084
RsaI GTAC 6 cut(s) 211, 1140, 1225, 1340, 2780, 3128
RsaNI GTAC 6 cut(s) 210, 1139, 1224, 1339, 2779, 3127
RseI CAYNNNNRTG 5 cut(s) 771, 1029, 2124, 2979, 3291
SapI GCTCTTC 1 cut(s) 3165
SchI GAGTC 4 cut(s) 1451, 1666, 2464, 3350
ScrFI CCNGG 6 cut(s) 306, 333, 1343, 1431, 1701, 1751
SduI GDGCHC 8 cut(s) 199, 305, 919, 1130, 1563, 2280, 3080, 3216
SfcI CTRYAG 5 cut(s) 460, 1761, 2643, 2685, 3395
SgrAI CRCCGGYG 1 cut(s) 1606
SinI GGWCC 7 cut(s) 328, 1072, 1697, 1704, 2166, 2496, 2825
SmiMI CAYNNNNRTG 5 cut(s) 771, 1029, 2124, 2979, 3291
SmlI CTYRAG 4 cut(s) 1076, 1720, 2237, 2248
SmoI CTYRAG 4 cut(s) 1076, 1720, 2237, 2248
SnaBI TACGTA 1 cut(s) 2585
SpeI ACTAGT 1 cut(s) 3316
SphI GCATGC 1 cut(s) 835
SsiI CCGC 7 cut(s) 452, 682, 907, 1061, 1070, 2280, 2885
StyD4I CCNGG 6 cut(s) 304, 331, 1341, 1429, 1699, 1749
StyI CCWWGG 7 cut(s) 153, 1332, 1521, 2088, 2868, 2947, 3004
TaaI ACNGT 7 cut(s) 139, 273, 316, 524, 1145, 1567, 2303
TaiI ACGT 3 cut(s) 260, 2587, 3355
TaqI TCGA 7 cut(s) 254, 1230, 1389, 1656, 2458, 3135, 3163
TatI WGTACW 2 cut(s) 1138, 1223
TfiI GAWTC 6 cut(s) 1106, 1244, 1682, 1814, 2573, 3341
TscAI CASTG 9 cut(s) 527, 823, 1135, 1699, 1816, 1834, 1909, 2974, 3160
TseFI GTSAC 5 cut(s) 258, 928, 1669, 1804, 3353
Tsp45I GTSAC 5 cut(s) 258, 928, 1669, 1804, 3353
TspGWI ACGGA 1 cut(s) 1865
TspRI CASTG 9 cut(s) 527, 823, 1135, 1699, 1816, 1834, 1909, 2974, 3160
Van91I CCANNNNNTGG 1 cut(s) 2602
VpaK11BI GGWCC 7 cut(s) 328, 1072, 1697, 1704, 2166, 2496, 2825
VspI ATTAAT 3 cut(s) 1733, 2133, 3111
XapI RAATTY 8 cut(s) 114, 672, 733, 872, 900, 1929, 2051, 2510
XbaI TCTAGA 2 cut(s) 2360, 3344
XceI RCATGY 6 cut(s) 42, 54, 835, 1030, 1100, 3043
XcmI CCANNNNNNNNNTGG 4 cut(s) 94, 735, 2209, 3137
XmnI GAANNNNTTC 3 cut(s) 711, 1929, 3198
ZraI GACGTC 1 cut(s) 258
Zsp2I ATGCAT 3 cut(s) 247, 989, 2835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.