pycom07g16040

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007094
Physical Location & Seq
Forward (+)
18536659 .. 18538808
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGTCCGGTCGAATGCTGAAGAAAGTTTTGAGGGAACAAGAAGAAGAACACGGACACGTAATAGAATCCGAATCAGAAGGCGAAGAAGAAAGCCAGCAGCTCAACGGAAAAGCTAAATTCAATCCCTTTGATCTCCTCAACGAGGACGGGGACAACGACGGCGACCACCAGGGGGATGAACCTGAAACTGTTGATGAACACTTGGTTGTTGTGCCTGAGCTGAAAACGAAGGTCGGTGTAGCGTCAACCAACAATCAGAAATCAAAGAAGAAGAAAAAGAAGAAAAGCAAAGAGGCTTCTTATTCTTCTTCGGGTGCTTCCAATGTAGAAAAGCCCCTGGATGTGATTCTTGAGTCGATCTCTTTGGATGGCCCCGAAAAAGCAGCCAGCGGAAAAGGTTGTGAGAATTTGGCTAAGCCATGTGCGCCCTCTGCGTTACAAGTGGATCCCAAGTACTTAAATGCTGAAAATGAGCTGCGGAGAATCTTTGGCTCTAAGGTGGTAAAGTCCTTTGAGAAACATGAGCGAAGTGGTGGCAGTTCTAGAATGGTGCGTGGTGGAAGACGTGCTGCTCATATTCCTAGAAAAACTATACTTGTCACTGCATCAGAGCATTGGCATCGCTGGGATGGTTCTTTGTCCATGGAATTTCTTGGAGTGATTGATGGATACCACCACTTTCGATATGTACATTCCTCATCCTACCTTCAAGCTCAGAGGGCATTTGAAGCTGCCAAAGCTACACATGACATCAATGGAATTGCAAGTATTTTGTTATACAGTCCTTATCATTTGGATTCGCTTATAACCATGGCAGAGTACTATAAGTTTTCGGGTGAACATCAAATATCAGCAGAACTTATCTCGAAGTGCTTATATGCTTTGGAATGTGCATGGAATCCAATGTTCACCCCCTTGCAGGGCAATTGCCAACTAAAATACAGCCATGAAACAAACAAGCCTCTGTTCACTGTACTTTTTACTCACATGAGAAATATGGATAGGCGCGGTTGCCATCGATCGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

343

Amino Acids

38.42

Weight (kDa)

7.0

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 227 - 340 2.6e-29 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AccII CGCG 1 cut(s) 1008
AciI CCGC 3 cut(s) 390, 478, 1008
AclWI GGATC 2 cut(s) 440, 453
AcsI RAATTY 3 cut(s) 116, 406, 647
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 4 cut(s) 455, 690, 821, 975
AfiI CCNNNNNNNGG 4 cut(s) 142, 172, 919, 920
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 3 cut(s) 121, 710, 728
AjiI CACGTC 1 cut(s) 566
AjnI CCWGG 2 cut(s) 168, 336
AluBI AGCT 7 cut(s) 100, 113, 220, 475, 713, 731, 740
AluI AGCT 7 cut(s) 100, 113, 220, 475, 713, 731, 740
AlwI GGATC 2 cut(s) 440, 453
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 5 cut(s) 97, 383, 475, 569, 731
ApoI RAATTY 3 cut(s) 116, 406, 647
AspLEI GCGC 2 cut(s) 427, 1008
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 848, 901
BamHI GGATCC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 568
BbvI GCAGC 5 cut(s) 109, 395, 462, 556, 718
BccI CCATC 4 cut(s) 362, 623, 659, 1023
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 2 cut(s) 170, 338
BciVI GTATCC 1 cut(s) 662
BfaI CTAG 3 cut(s) 543, 582, 1027
BfuI GTATCC 1 cut(s) 662
BisI GCNGC 5 cut(s) 98, 384, 476, 570, 732
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 5 cut(s) 99, 385, 477, 571, 733
BmcAI AGTACT 2 cut(s) 455, 821
Bme1390I CCNGG 2 cut(s) 170, 338
BmgBI CACGTC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 373, 447
BmrFI CCNGG 2 cut(s) 170, 338
BmsI GCATC 2 cut(s) 614, 628
BpiI GAAGAC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 344, 376
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 371
Bsa29I ATCGAT 2 cut(s) 1018, 1022
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 4 cut(s) 169, 336, 642, 810
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 172, 919, 920
BseBI CCWGG 2 cut(s) 170, 338
BseCI ATCGAT 2 cut(s) 1018, 1022
BseDI CCNNGG 4 cut(s) 169, 336, 642, 810
BseGI GGATG 5 cut(s) 181, 346, 373, 634, 698
BseLI CCNNNNNNNGG 4 cut(s) 142, 172, 919, 920
BseMII CTCAG 2 cut(s) 207, 728
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 5 cut(s) 109, 395, 462, 556, 718
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 1008
Bsh1285I CGRYCG 2 cut(s) 10, 1022
BshFI GGCC 1 cut(s) 372
BshVI ATCGAT 2 cut(s) 1018, 1022
BsiEI CGRYCG 2 cut(s) 10, 1022
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 4 cut(s) 142, 172, 919, 920
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 688
Bsp143I GATC 5 cut(s) 130, 357, 445, 1019, 1023
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 642, 810
BspACI CCGC 3 cut(s) 390, 478, 1008
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 2 cut(s) 208, 727
BspDI ATCGAT 2 cut(s) 1018, 1022
BspFNI CGCG 1 cut(s) 1008
BspLI GGNNCC 2 cut(s) 373, 447
BspPI GGATC 2 cut(s) 440, 453
BsrGI TGTACA 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 169, 336, 642, 810
BssMI GATC 5 cut(s) 130, 357, 445, 1019, 1023
BssT1I CCWWGG 2 cut(s) 642, 810
Bst2UI CCWGG 2 cut(s) 170, 338
Bst4CI ACNGT 3 cut(s) 190, 782, 973
BstAUI TGTACA 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 95, 388
BstDEI CTNAG 4 cut(s) 216, 414, 495, 714
BstDSI CCRYGG 2 cut(s) 642, 810
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 5 cut(s) 181, 346, 373, 634, 698
BstFNI CGCG 1 cut(s) 1008
BstHHI GCGC 2 cut(s) 427, 1008
BstKTI GATC 5 cut(s) 133, 360, 448, 1022, 1026
BstMBI GATC 5 cut(s) 130, 357, 445, 1019, 1023
BstMCI CGRYCG 2 cut(s) 10, 1022
BstMWI GCNNNNNNNGC 5 cut(s) 424, 431, 719, 728, 737
BstNI CCWGG 2 cut(s) 170, 338
BstSCI CCNGG 2 cut(s) 168, 336
BstUI CGCG 1 cut(s) 1008
BstV1I GCAGC 5 cut(s) 109, 395, 462, 556, 718
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
Bsu15I ATCGAT 2 cut(s) 1018, 1022
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 1 cut(s) 372
BsuTUI ATCGAT 2 cut(s) 1018, 1022
BtgI CCRYGG 2 cut(s) 642, 810
BtgZI GCGATG 1 cut(s) 605
BtrI CACGTC 1 cut(s) 566
BtsCI GGATG 5 cut(s) 181, 346, 373, 634, 698
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 600, 969
Cac8I GCNNGC 2 cut(s) 95, 388
CfoI GCGC 2 cut(s) 427, 1008
Cfr13I GGNCC 1 cut(s) 371
ClaI ATCGAT 2 cut(s) 1018, 1022
CseI GACGC 1 cut(s) 231
Csp6I GTAC 4 cut(s) 454, 689, 820, 974
CviAII CATG 8 cut(s) 420, 521, 643, 746, 811, 894, 947, 988
CviQI GTAC 4 cut(s) 454, 689, 820, 974
DdeI CTNAG 4 cut(s) 216, 414, 495, 714
DpnI GATC 5 cut(s) 132, 359, 447, 1021, 1025
DpnII GATC 5 cut(s) 130, 357, 445, 1019, 1023
Eco130I CCWWGG 2 cut(s) 642, 810
Eco57I CTGAAG 1 cut(s) 38
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 168, 336
EcoT14I CCWWGG 2 cut(s) 642, 810
ErhI CCWWGG 2 cut(s) 642, 810
FaeI CATG 8 cut(s) 423, 524, 646, 749, 814, 897, 950, 991
FaqI GGGAC 1 cut(s) 164
FatI CATG 8 cut(s) 419, 520, 642, 745, 810, 893, 946, 987
Fnu4HI GCNGC 5 cut(s) 98, 384, 476, 570, 732
FokI GGATG 5 cut(s) 188, 353, 380, 641, 685
Fsp4HI GCNGC 5 cut(s) 98, 384, 476, 570, 732
FspBI CTAG 3 cut(s) 543, 582, 1027
GlaI GCGC 2 cut(s) 426, 1007
GluI GCNGC 5 cut(s) 98, 384, 476, 570, 732
GsaI CCCAGC 1 cut(s) 628
HaeIII GGCC 1 cut(s) 372
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 231
HhaI GCGC 2 cut(s) 427, 1008
Hin1II CATG 8 cut(s) 423, 524, 646, 749, 814, 897, 950, 991
Hin6I GCGC 2 cut(s) 425, 1006
HinP1I GCGC 2 cut(s) 425, 1006
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 7 cut(s) 65, 71, 346, 353, 483, 797, 898
HpaII CCGG 1 cut(s) 6
HphI GGTGA 2 cut(s) 848, 901
Hpy166II GTNNAC 4 cut(s) 246, 839, 909, 969
Hpy188I TCNGA 5 cut(s) 70, 76, 258, 610, 717
Hpy188III TCNNGA 3 cut(s) 350, 543, 865
Hpy8I GTNNAC 4 cut(s) 246, 839, 909, 969
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 71, 223, 716
HpyCH4III ACNGT 3 cut(s) 190, 782, 973
HpyCH4IV ACGT 2 cut(s) 57, 565
HpyCH4V TGCA 4 cut(s) 605, 764, 893, 919
HpyF10VI GCNNNNNNNGC 5 cut(s) 424, 431, 719, 728, 737
HpyF3I CTNAG 4 cut(s) 216, 414, 495, 714
HpySE526I ACGT 2 cut(s) 57, 565
Hsp92II CATG 8 cut(s) 423, 524, 646, 749, 814, 897, 950, 991
HspAI GCGC 2 cut(s) 425, 1006
Kzo9I GATC 5 cut(s) 130, 357, 445, 1019, 1023
Lsp1109I GCAGC 5 cut(s) 109, 395, 462, 556, 718
LweI GCATC 2 cut(s) 614, 628
MaeI CTAG 3 cut(s) 543, 582, 1027
MaeII ACGT 2 cut(s) 57, 565
MaeIII GTNAC 2 cut(s) 435, 598
MalI GATC 5 cut(s) 132, 359, 447, 1021, 1025
MboI GATC 5 cut(s) 130, 357, 445, 1019, 1023
MfeI CAATTG 1 cut(s) 925
MflI RGATCY 1 cut(s) 445
MluCI AATT 5 cut(s) 116, 406, 647, 759, 925
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 8 cut(s) 24, 136, 146, 286, 439, 706, 711, 972
MseI TTAA 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 2 cut(s) 170, 338
MunI CAATTG 1 cut(s) 925
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 2 cut(s) 170, 338
MvnI CGCG 1 cut(s) 1008
MwoI GCNNNNNNNGC 5 cut(s) 424, 431, 719, 728, 737
NcoI CCATGG 2 cut(s) 642, 810
NdeII GATC 5 cut(s) 130, 357, 445, 1019, 1023
NlaIII CATG 8 cut(s) 423, 524, 646, 749, 814, 897, 950, 991
NlaIV GGNNCC 2 cut(s) 373, 447
NmuCI GTSAC 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 6 cut(s) 65, 71, 346, 483, 797, 898
PkrI GCNGC 5 cut(s) 99, 385, 477, 571, 733
Ple19I CGATCG 1 cut(s) 1022
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 58
PsiI TTATAA 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 168, 336
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 2 cut(s) 168, 336
PspN4I GGNNCC 2 cut(s) 373, 447
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 1 cut(s) 445
PvuI CGATCG 1 cut(s) 1022
RsaI GTAC 4 cut(s) 455, 690, 821, 975
RsaNI GTAC 4 cut(s) 454, 689, 820, 974
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 5 cut(s) 98, 384, 476, 570, 732
Sau3AI GATC 5 cut(s) 130, 357, 445, 1019, 1023
Sau96I GGNCC 1 cut(s) 371
ScaI AGTACT 2 cut(s) 455, 821
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 170, 338
SfaNI GCATC 2 cut(s) 614, 628
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 5 cut(s) 116, 406, 647, 759, 925
SsiI CCGC 3 cut(s) 390, 478, 1008
SspMI CTAG 3 cut(s) 543, 582, 1027
StyD4I CCNGG 2 cut(s) 168, 336
StyI CCWWGG 2 cut(s) 642, 810
TaaI ACNGT 3 cut(s) 190, 782, 973
TaiI ACGT 2 cut(s) 60, 568
TaqI TCGA 6 cut(s) 10, 356, 682, 866, 1018, 1022
TasI AATT 5 cut(s) 116, 406, 647, 759, 925
TatI WGTACW 4 cut(s) 453, 688, 819, 973
TfiI GAWTC 6 cut(s) 65, 71, 346, 483, 797, 898
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 2 cut(s) 607, 976
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 5 cut(s) 97, 383, 475, 569, 731
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 3 cut(s) 192, 210, 963
TspGWI ACGGA 2 cut(s) 66, 120
TspRI CASTG 2 cut(s) 607, 976
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 3 cut(s) 116, 406, 647
XbaI TCTAGA 1 cut(s) 542
XspI CTAG 3 cut(s) 543, 582, 1027
ZrmI AGTACT 2 cut(s) 455, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.