pycom07g18090

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003808
Physical Location & Seq
Reverse (-)
20590568 .. 20592321
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGGTGAAGCGAAGAACCTACCCTTCATGTAAACTTAGTCGAATAGCTAGAGGTATGGAGAAGGAAATAGCAGATGATTTGTTTGGCAAATTACCAAATGAAATCATTTGCAGAATTATTTCTTTTCTCCCTTCAGAATCTGCATTAGAAACCAGTTTAATCTCAAGTATGTGGAGAGGCCTATGGAATACAACTTTAGCGAAGCAGATCACTACAGATGATGATGCAGCTGATGCAGTTTTTGAATTTGTCTCTCACTTTAACCAGCATGATCCCTTAGGGCATCCCAGAAAGCTGCAACTGCACTTCGGCAAACTTGGTGATCTCCTTTTGGCTACAGTTTCACCTAACAATAAACTCCGTCTGGAGTTTTCGACAGAGAATCGGGAAACTCCAAGGCATTTTGGCTGGCACTTGAAGCTGAATCACCAAAGGGACCAAACCCTAGTTTCTCACCAGCCTTCTCCCTCTACTTTCTTTGTCAAATTTCTTTACCTAAAATCAGTGAGCTCTTTTGGAAATGAAGCAGTTTCTTCTATGATTCCGTGTTTCGAATTTCTCGAAAACTTGAAGATCATTGAATGTGAGGAGTTACAATCTTTGCATATCGAGTCCAGTCCAAGGCTACTTAGCTTGACCATCTTTGACTGCCAGAATCTCAAGTCTCTCCACCTCAAAACTTCCAAACTTCGATCTTTTCGATACCGCGGTTTTCTCCCTCATATTTGGACGGAACATCATTTTAACTTGGCAGACGCCATGCTTGATTTCAGGCAAGGCCCTGGCTACGGCGGATTCAAAAGTAGCGACTTTGATCCTATGTTGTTAACCATTAAAAATGCTCAAGTTCTCACTCTATGCAAATGGACTTTCCAGACACTGATCTGGCCATCCCTTTTGCCTCTACAAGCAAACTTCATATTCTACAAGCTGAAAGAGCTATGGTGGATTGATAACTGGGAAGAAAGATTCAGCATCGATCCTCTAATATCCTTCATTAAATTATGTCCAGCTTTGGAGCAACTCTATGTAACTAATGATCCTAAAAGCTACTGCATGGCATGGACAGCTCCACCGTGGAAGAAAGTCAATGGCCAAACAAAATTGGCCCATCTGAAAGTGATAAAGTTGGTGGGGTTTACTACTGAAGATGATGAAACCACTTTGGCAGAGCATATAAGAGATCTAGTTACAGTGGAGCCACTGATAATTAGCAGCATCAAGACTGAGAAAAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

414

Amino Acids

47.77

Weight (kDa)

8.05

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 163 - 393 3.9e-10 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 708
AciI CCGC 3 cut(s) 706, 708, 792
AclWI GGATC 4 cut(s) 266, 809, 974, 1034
AcoI YGGCCR 2 cut(s) 887, 1093
AcsI RAATTY 3 cut(s) 245, 485, 554
AcuI CTGAAG 2 cut(s) 117, 1167
AcyI GRCGYC 1 cut(s) 756
AfiI CCNNNNNNNGG 2 cut(s) 621, 788
AgsI TTSAA 5 cut(s) 245, 418, 571, 581, 799
AjnI CCWGG 1 cut(s) 781
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 2 cut(s) 256, 669
AlwI GGATC 4 cut(s) 266, 809, 974, 1034
AlwNI CAGNNNCTG 2 cut(s) 140, 880
AoxI GGCC 5 cut(s) 178, 778, 887, 1093, 1107
ApeKI GCWGC 3 cut(s) 227, 295, 1215
ApoI RAATTY 3 cut(s) 245, 485, 554
Asp700I GAANNNNTTC 1 cut(s) 118
AspS9I GGNCC 3 cut(s) 436, 779, 1108
AsuHPI GGTGA 5 cut(s) 16, 332, 336, 419, 446
AsuII TTCGAA 1 cut(s) 552
AvaII GGWCC 1 cut(s) 436
AxyI CCTNAGG 1 cut(s) 277
BaeI ACNNNNGTAYC 2 cut(s) 694, 727
BalI TGGCCA 2 cut(s) 889, 1095
BanII GRGCYC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 3 cut(s) 239, 282, 1227
BccI CCATC 3 cut(s) 647, 898, 1119
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 1 cut(s) 783
BcoDI GTCTC 2 cut(s) 256, 669
BfaI CTAG 3 cut(s) 48, 446, 1187
BfmI CTRYAG 2 cut(s) 213, 336
BglII AGATCT 1 cut(s) 1183
BisI GCNGC 3 cut(s) 228, 296, 1216
BlsI GCNGC 3 cut(s) 229, 297, 1217
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 3 cut(s) 436, 779, 1108
BmiI GGNNCC 2 cut(s) 437, 1200
BmrFI CCNGG 1 cut(s) 783
BmrI ACTGGG 1 cut(s) 967
BmsI GCATC 5 cut(s) 214, 223, 292, 984, 1227
BmuI ACTGGG 1 cut(s) 967
BpmI CTGGAG 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 3 cut(s) 148, 644, 828
Bsa29I ATCGAT 1 cut(s) 978
BsaHI GRCGYC 1 cut(s) 756
BsaJI CCNNGG 5 cut(s) 395, 620, 706, 781, 1076
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 788
Bse1I ACTGG 3 cut(s) 153, 615, 962
Bse21I CCTNAGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 783
BseCI ATCGAT 1 cut(s) 978
BseDI CCNNGG 5 cut(s) 395, 620, 706, 781, 1076
BseGI GGATG 2 cut(s) 283, 890
BseLI CCNNNNNNNGG 2 cut(s) 621, 788
BseMII CTCAG 1 cut(s) 1218
BseNI ACTGG 3 cut(s) 153, 615, 962
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 3 cut(s) 239, 282, 1227
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 5 cut(s) 180, 780, 889, 1095, 1109
BshVI ATCGAT 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 512
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 2 cut(s) 621, 788
BsmAI GTCTC 2 cut(s) 256, 669
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 5 cut(s) 180, 780, 889, 1095, 1109
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 512
BspACI CCGC 3 cut(s) 706, 708, 792
BspANI GGCC 5 cut(s) 180, 780, 889, 1095, 1109
BspCNI CTCAG 1 cut(s) 1219
BspDI ATCGAT 1 cut(s) 978
BspFNI CGCG 1 cut(s) 708
BspLI GGNNCC 2 cut(s) 437, 1200
BspPI GGATC 4 cut(s) 266, 809, 974, 1034
BspT104I TTCGAA 1 cut(s) 552
BsrI ACTGG 3 cut(s) 153, 615, 962
BssECI CCNNGG 5 cut(s) 395, 620, 706, 781, 1076
BssNI GRCGYC 1 cut(s) 756
BssT1I CCWWGG 2 cut(s) 395, 620
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 3 cut(s) 340, 1077, 1195
BstACI GRCGYC 1 cut(s) 756
BstAPI GCANNNNNTGC 1 cut(s) 233
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 4 cut(s) 35, 277, 629, 1227
BstDSI CCRYGG 2 cut(s) 706, 1076
BstF5I GGATG 2 cut(s) 283, 890
BstFNI CGCG 1 cut(s) 708
BstMAI GTCTC 2 cut(s) 256, 669
BstMWI GCNNNNNNNGC 5 cut(s) 233, 301, 418, 937, 1067
BstNI CCWGG 1 cut(s) 783
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 2 cut(s) 213, 336
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 3 cut(s) 239, 282, 1227
BstX2I RGATCY 1 cut(s) 1183
BstYI RGATCY 1 cut(s) 1183
Bsu15I ATCGAT 1 cut(s) 978
Bsu36I CCTNAGG 1 cut(s) 277
BsuRI GGCC 5 cut(s) 180, 780, 889, 1095, 1109
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 2 cut(s) 706, 1076
BtsCI GGATG 2 cut(s) 283, 890
BtsIMutI CAGTG 4 cut(s) 510, 878, 1200, 1202
Cac8I GCNNGC 1 cut(s) 410
CaiI CAGNNNCTG 2 cut(s) 140, 880
Cfr13I GGNCC 3 cut(s) 436, 779, 1108
Cfr42I CCGCGG 1 cut(s) 709
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 1 cut(s) 764
CviAII CATG 5 cut(s) 27, 269, 760, 1057, 1062
DdeI CTNAG 4 cut(s) 35, 277, 629, 1227
EaeI YGGCCR 2 cut(s) 887, 1093
EciI GGCGGA 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 2 cut(s) 395, 620
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 2 cut(s) 117, 1167
Eco81I CCTNAGG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 779
EcoRII CCWGG 1 cut(s) 781
EcoT14I CCWWGG 2 cut(s) 395, 620
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 2 cut(s) 395, 620
FaeI CATG 5 cut(s) 30, 272, 763, 1060, 1065
FaqI GGGAC 1 cut(s) 449
FatI CATG 5 cut(s) 26, 268, 759, 1056, 1061
Fnu4HI GCNGC 3 cut(s) 228, 296, 1216
FokI GGATG 2 cut(s) 270, 877
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 3 cut(s) 228, 296, 1216
FspBI CTAG 3 cut(s) 48, 446, 1187
GluI GCNGC 3 cut(s) 228, 296, 1216
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 5 cut(s) 180, 780, 889, 1095, 1109
HgaI GACGC 1 cut(s) 764
Hin1I GRCGYC 1 cut(s) 756
Hin1II CATG 5 cut(s) 30, 272, 763, 1060, 1065
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HinfI GANTC 8 cut(s) 137, 382, 424, 541, 611, 655, 795, 969
HpaI GTTAAC 1 cut(s) 828
HphI GGTGA 5 cut(s) 16, 332, 336, 419, 446
Hpy166II GTNNAC 3 cut(s) 32, 828, 1140
Hpy188I TCNGA 2 cut(s) 136, 1116
Hpy188III TCNNGA 5 cut(s) 365, 386, 560, 874, 1222
Hpy8I GTNNAC 3 cut(s) 32, 828, 1140
HpyAV CCTTC 5 cut(s) 33, 55, 141, 471, 1003
HpyCH4III ACNGT 3 cut(s) 340, 1077, 1195
HpyCH4V TGCA 9 cut(s) 111, 143, 227, 236, 298, 304, 604, 861, 1056
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 301, 418, 937, 1067
HpyF3I CTNAG 4 cut(s) 35, 277, 629, 1227
Hsp92I GRCGYC 1 cut(s) 756
Hsp92II CATG 5 cut(s) 30, 272, 763, 1060, 1065
KspAI GTTAAC 1 cut(s) 828
KspI CCGCGG 1 cut(s) 709
LmnI GCTCC 3 cut(s) 1018, 1075, 1198
Lsp1109I GCAGC 3 cut(s) 239, 282, 1227
LweI GCATC 5 cut(s) 214, 223, 292, 984, 1227
MaeI CTAG 3 cut(s) 48, 446, 1187
MaeIII GTNAC 3 cut(s) 591, 1030, 1189
MboII GAAGA 6 cut(s) 24, 525, 583, 974, 1093, 1160
MflI RGATCY 1 cut(s) 1183
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 2 cut(s) 889, 1095
MluCI AATT 8 cut(s) 89, 114, 245, 485, 554, 1001, 1103, 1209
MluNI TGGCCA 2 cut(s) 889, 1095
MlyI GAGTC 1 cut(s) 620
MnlI CCTC 8 cut(s) 44, 170, 478, 580, 683, 729, 912, 993
Mox20I TGGCCA 2 cut(s) 889, 1095
MroXI GAANNNNTTC 1 cut(s) 118
MscI TGGCCA 2 cut(s) 889, 1095
MseI TTAA 6 cut(s) 158, 261, 744, 827, 834, 999
Msp20I TGGCCA 2 cut(s) 889, 1095
MspA1I CMGCKG 2 cut(s) 230, 708
MspR9I CCNGG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 783
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 5 cut(s) 233, 301, 418, 937, 1067
NlaIII CATG 5 cut(s) 30, 272, 763, 1060, 1065
NlaIV GGNNCC 2 cut(s) 437, 1200
NspV TTCGAA 1 cut(s) 552
PceI AGGCCT 1 cut(s) 180
PcsI WCGNNNNNNNCGW 2 cut(s) 558, 697
PdmI GAANNNNTTC 1 cut(s) 118
PfeI GAWTC 7 cut(s) 137, 382, 424, 541, 655, 795, 969
PkrI GCNGC 3 cut(s) 229, 297, 1217
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 2 cut(s) 437, 1200
PspPI GGNCC 3 cut(s) 436, 779, 1108
PstNI CAGNNNCTG 2 cut(s) 140, 880
PsuI RGATCY 1 cut(s) 1183
PvuII CAGCTG 1 cut(s) 230
SacI GAGCTC 1 cut(s) 512
SacII CCGCGG 1 cut(s) 709
SaqAI TTAA 6 cut(s) 158, 261, 744, 827, 834, 999
SatI GCNGC 3 cut(s) 228, 296, 1216
Sau96I GGNCC 3 cut(s) 436, 779, 1108
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 5 cut(s) 214, 223, 292, 984, 1227
SfcI CTRYAG 2 cut(s) 213, 336
Sfr303I CCGCGG 1 cut(s) 709
SfuI TTCGAA 1 cut(s) 552
SgrBI CCGCGG 1 cut(s) 709
SinI GGWCC 1 cut(s) 436
SmlI CTYRAG 3 cut(s) 163, 659, 843
SmoI CTYRAG 3 cut(s) 163, 659, 843
Sse9I AATT 8 cut(s) 89, 114, 245, 485, 554, 1001, 1103, 1209
SseBI AGGCCT 1 cut(s) 180
SsiI CCGC 3 cut(s) 706, 708, 792
SspMI CTAG 3 cut(s) 48, 446, 1187
SstI GAGCTC 1 cut(s) 512
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 781
StyI CCWWGG 2 cut(s) 395, 620
TaaI ACNGT 3 cut(s) 340, 1077, 1195
TaqI TCGA 8 cut(s) 40, 374, 552, 561, 609, 691, 700, 978
TasI AATT 8 cut(s) 89, 114, 245, 485, 554, 1001, 1103, 1209
TfiI GAWTC 7 cut(s) 137, 382, 424, 541, 655, 795, 969
Tru1I TTAA 6 cut(s) 158, 261, 744, 827, 834, 999
Tru9I TTAA 6 cut(s) 158, 261, 744, 827, 834, 999
TscAI CASTG 4 cut(s) 510, 885, 1200, 1209
TseI GCWGC 3 cut(s) 227, 295, 1215
TspDTI ATGAA 6 cut(s) 15, 114, 537, 907, 985, 1170
TspGWI ACGGA 3 cut(s) 350, 534, 746
TspRI CASTG 4 cut(s) 510, 885, 1200, 1209
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 3 cut(s) 245, 485, 554
XmnI GAANNNNTTC 1 cut(s) 118
XspI CTAG 3 cut(s) 48, 446, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.