pycom07g18110

nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002733
Physical Location & Seq
Forward (+)
20609742 .. 20613121
3380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18110.2

Sequence Viewer

Length: 1179 bp
ATGGGACCCATCAGAGGGTCCAGGAAGAAGAAGAAGGTTGACCAGAATGTGGTAGCTGCTTCAGAATTGCAGCCCCTGGAATGTTGGTGGGACGGCTTCTCTCAGAGAATTACTGTTCTCTCCAGTGTTCTAGGTCAATCGAAATCTAAAGGTACATTGAAATTTGAATCTTTCTTCAAATTTTCGGGAAAGACATTCAGCTACATCTGTTCACTTGTAAAGGAAGATATGATGGCCAGGTCCTCAACCTTCTGCTACTCGAATGGCAAGCCTTTGTGTCTAAATGACCAAGTTGCTATTGCTCTTAGGAGGCTTAGCTCCGGTGATTCATTATGCAGCATTGGCGAGTGCTTTGGGATGAACCAGTCAACTGTCTCGCACATAACCTGGCGTTTTGTGGAAGTAATGGAAGAAAGAGGGCTGCACCATCTTTCCTGGCCTAAAGAGCAGGGTGAAATGGAGGAGGTAAAGTCCAAGTTTGAGAAAATCCGCGGCCTTCCAAATTGTTGCGGCGCAATTGATATCACGCACATCATGATGACTCTGCCTACAGCCGACTCATCAGATGATATCTGGCTCGATGGTGAGAAAAACTGCAGCATGATCTTGCAGGCAATCGTTGATCCAGACATGAGGTTTCGTAACATAACTACAGGATGGCCAGGAAGAATGAGCGATGACATTGTCCTCCGTAGCTCAGGTTTTTTCAAGATGTCCGAAGAAGGAGATTGTTTAAACGGGGAGAAGTTGGTGCTCTCAGAAGGAACAGAAGTGAGAGAATACATAGTAGGAGATTCGGGTTTTCCTCTCTTGCCGTGGCTTCTCACTCCTTACAAGGGAAGAGGGCTCTGGGAGCATGAGTCTGAGTTCAACAAGCGGATTTCTGCAACACAGATGGTGGCGCAAAGGGCGTTGGTGAGGCTGAAAGAGATGTGGAAGATAATTCAAGGTGTAATGTGGAAGCCGGATAAGAACAAGCTGCCGAGGATTATTCTTGTTTGCTGTATACTGCACAATATAGTGATTGATATGGAGGATGAGGTGCAAGATGAAATGCCATCGTCCCGCCATCATGATCCTGGTTACCAGCAACAAATTTGTGAATCTGCTGCTGACGATACGGCATTTCACCTGAGAGAGAAGCTCTCTCTATACTTAGCCGGAAAATTGTCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

393

Amino Acids

44.51

Weight (kDa)

6.34

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 1 cut(s) 1006
AccII CGCG 1 cut(s) 492
AciI CCGC 5 cut(s) 490, 492, 510, 877, 1066
AclWI GGATC 2 cut(s) 617, 1070
AcoI YGGCCR 2 cut(s) 234, 659
AcsI RAATTY 3 cut(s) 161, 179, 1095
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 4 cut(s) 14, 15, 49, 836
AgsI TTSAA 6 cut(s) 160, 167, 178, 709, 871, 947
AjnI CCWGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
AluBI AGCT 6 cut(s) 56, 201, 318, 696, 979, 1144
AluI AGCT 6 cut(s) 56, 201, 318, 696, 979, 1144
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 2 cut(s) 617, 1070
AlwNI CAGNNNCTG 1 cut(s) 76
AoxI GGCC 4 cut(s) 234, 437, 493, 659
ApeKI GCWGC 7 cut(s) 56, 70, 336, 421, 597, 979, 1109
ApoI RAATTY 3 cut(s) 161, 179, 1095
AspLEI GCGC 2 cut(s) 515, 904
AspS9I GGNCC 3 cut(s) 5, 18, 240
AsuHPI GGTGA 5 cut(s) 335, 464, 596, 928, 1121
AvaII GGWCC 3 cut(s) 5, 18, 240
BalI TGGCCA 2 cut(s) 236, 661
BanII GRGCYC 1 cut(s) 849
Bbv12I GWGCWC 1 cut(s) 756
BbvI GCAGC 7 cut(s) 43, 82, 348, 408, 609, 966, 1096
BccI CCATC 8 cut(s) 17, 226, 435, 575, 651, 889, 1066, 1077
BceAI ACGGC 3 cut(s) 109, 799, 1137
BciT130I CCWGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 3 cut(s) 549, 595, 651
BisI GCNGC 9 cut(s) 57, 71, 337, 422, 493, 511, 598, 980, 1110
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 9 cut(s) 58, 72, 338, 423, 494, 512, 599, 981, 1111
Bme1390I CCNGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
Bme18I GGWCC 3 cut(s) 5, 18, 240
BmgT120I GGNCC 3 cut(s) 5, 18, 240
BmiI GGNNCC 3 cut(s) 6, 7, 19
BmrFI CCNGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
BplI GAGNNNNNCTC 4 cut(s) 1128, 1160, 1130, 1162
BpmI CTGGAG 1 cut(s) 106
Bpu10I CCTNAGC 1 cut(s) 697
Bpu1102I GCTNAGC 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 75, 490, 815, 983
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 4 cut(s) 455, 485, 845, 875
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 15, 49, 836
Bse1I ACTGG 2 cut(s) 123, 364
BseBI CCWGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
BseDI CCNNGG 4 cut(s) 75, 490, 815, 983
BseGI GGATG 3 cut(s) 363, 662, 1042
BseLI CCNNNNNNNGG 4 cut(s) 14, 15, 49, 836
BseMII CTCAG 5 cut(s) 116, 711, 771, 855, 1124
BseNI ACTGG 2 cut(s) 123, 364
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 7 cut(s) 43, 82, 348, 408, 609, 966, 1096
BsgI GTGCAG 2 cut(s) 407, 995
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 4 cut(s) 236, 439, 495, 661
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 3 cut(s) 321, 965, 1161
BslFI GGGAC 3 cut(s) 18, 104, 1048
BslI CCNNNNNNNGG 4 cut(s) 14, 15, 49, 836
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 3 cut(s) 18, 104, 1048
BsnI GGCC 4 cut(s) 236, 439, 495, 661
Bsp1286I GDGCHC 2 cut(s) 756, 849
Bsp143I GATC 3 cut(s) 603, 622, 1075
Bsp1720I GCTNAGC 1 cut(s) 314
BspACI CCGC 5 cut(s) 490, 492, 510, 877, 1066
BspANI GGCC 4 cut(s) 236, 439, 495, 661
BspCNI CTCAG 5 cut(s) 115, 710, 770, 856, 1125
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 2 cut(s) 534, 1072
BspLI GGNNCC 3 cut(s) 6, 7, 19
BspMAI CTGCAG 1 cut(s) 599
BspPI GGATC 2 cut(s) 617, 1070
BsrI ACTGG 2 cut(s) 123, 364
BssECI CCNNGG 4 cut(s) 75, 490, 815, 983
BssMI GATC 3 cut(s) 603, 622, 1075
BssNAI GTATAC 1 cut(s) 1007
Bst1107I GTATAC 1 cut(s) 1007
Bst2UI CCWGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
Bst4CI ACNGT 2 cut(s) 115, 373
Bst6I CTCTTC 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 269, 612
BstDEI CTNAG 8 cut(s) 102, 305, 314, 697, 757, 864, 1133, 1156
BstDSI CCRYGG 2 cut(s) 490, 815
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 3 cut(s) 363, 662, 1042
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 2 cut(s) 515, 904
BstKTI GATC 3 cut(s) 606, 625, 1078
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 3 cut(s) 603, 622, 1075
BstMWI GCNNNNNNNGC 4 cut(s) 342, 445, 853, 908
BstNI CCWGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
BstSFI CTRYAG 3 cut(s) 549, 595, 651
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 7 cut(s) 43, 82, 348, 408, 609, 966, 1096
BstZ17I GTATAC 1 cut(s) 1007
BsuRI GGCC 4 cut(s) 236, 439, 495, 661
BtgI CCRYGG 2 cut(s) 490, 815
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 3 cut(s) 363, 662, 1042
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 2 cut(s) 269, 612
CaiI CAGNNNCTG 1 cut(s) 76
CciI TCATGA 2 cut(s) 534, 1072
CfoI GCGC 2 cut(s) 515, 904
Cfr13I GGNCC 3 cut(s) 5, 18, 240
Cfr42I CCGCGG 1 cut(s) 493
Csp6I GTAC 1 cut(s) 153
CviAII CATG 5 cut(s) 535, 601, 631, 857, 1073
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 8 cut(s) 102, 305, 314, 697, 757, 864, 1133, 1156
DpnI GATC 3 cut(s) 605, 624, 1077
DpnII GATC 3 cut(s) 603, 622, 1075
DraI TTTAAA 1 cut(s) 735
EaeI YGGCCR 2 cut(s) 234, 659
Eam1104I CTCTTC 1 cut(s) 835
EarI CTCTTC 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 849
Eco32I GATATC 2 cut(s) 523, 571
Eco47I GGWCC 3 cut(s) 5, 18, 240
Eco57I CTGAAG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 1082
EcoO109I RGGNCCY 2 cut(s) 5, 240
EcoO65I GGTNACC 1 cut(s) 1082
EcoRII CCWGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
EcoRV GATATC 2 cut(s) 523, 571
EcoT38I GRGCYC 1 cut(s) 849
FaeI CATG 5 cut(s) 538, 604, 634, 860, 1076
FaqI GGGAC 3 cut(s) 18, 104, 1048
FatI CATG 5 cut(s) 534, 600, 630, 856, 1072
FauI CCCGC 1 cut(s) 1073
FblI GTMKAC 1 cut(s) 1006
Fnu4HI GCNGC 9 cut(s) 57, 71, 337, 422, 493, 511, 598, 980, 1110
FokI GGATG 3 cut(s) 370, 669, 1049
FriOI GRGCYC 1 cut(s) 849
Fsp4HI GCNGC 9 cut(s) 57, 71, 337, 422, 493, 511, 598, 980, 1110
FspBI CTAG 1 cut(s) 131
GlaI GCGC 2 cut(s) 514, 903
GluI GCNGC 9 cut(s) 57, 71, 337, 422, 493, 511, 598, 980, 1110
GsuI CTGGAG 1 cut(s) 106
HaeIII GGCC 4 cut(s) 236, 439, 495, 661
HapII CCGG 3 cut(s) 321, 965, 1161
HhaI GCGC 2 cut(s) 515, 904
Hin1II CATG 5 cut(s) 538, 604, 634, 860, 1076
Hin6I GCGC 2 cut(s) 513, 902
HinP1I GCGC 2 cut(s) 513, 902
HincII GTYRAC 2 cut(s) 40, 369
HindII GTYRAC 2 cut(s) 40, 369
HinfI GANTC 7 cut(s) 167, 326, 541, 557, 794, 860, 1103
HpaII CCGG 3 cut(s) 321, 965, 1161
HphI GGTGA 5 cut(s) 335, 464, 596, 928, 1121
Hpy166II GTNNAC 4 cut(s) 40, 212, 369, 1007
Hpy188I TCNGA 7 cut(s) 14, 64, 105, 565, 718, 760, 865
Hpy188III TCNNGA 6 cut(s) 186, 535, 626, 709, 1073, 1173
Hpy8I GTNNAC 4 cut(s) 40, 212, 369, 1007
HpyAV CCTTC 5 cut(s) 28, 259, 506, 716, 755
HpyCH4III ACNGT 2 cut(s) 115, 373
HpyCH4V TGCA 8 cut(s) 70, 336, 424, 597, 610, 887, 1012, 1045
HpyF10VI GCNNNNNNNGC 4 cut(s) 342, 445, 853, 908
HpyF3I CTNAG 8 cut(s) 102, 305, 314, 697, 757, 864, 1133, 1156
Hsp92II CATG 5 cut(s) 538, 604, 634, 860, 1076
HspAI GCGC 2 cut(s) 513, 902
KflI GGGWCCC 1 cut(s) 5
KspI CCGCGG 1 cut(s) 493
Kzo9I GATC 3 cut(s) 603, 622, 1075
LmnI GCTCC 2 cut(s) 323, 853
Lsp1109I GCAGC 7 cut(s) 43, 82, 348, 408, 609, 966, 1096
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 641, 1082
MalI GATC 3 cut(s) 605, 624, 1077
MboI GATC 3 cut(s) 603, 622, 1075
MfeI CAATTG 1 cut(s) 516
MhlI GDGCHC 2 cut(s) 756, 849
MlsI TGGCCA 2 cut(s) 236, 661
MluCI AATT 9 cut(s) 65, 108, 161, 179, 502, 516, 942, 1095, 1166
MluNI TGGCCA 2 cut(s) 236, 661
MlyI GAGTC 3 cut(s) 535, 551, 869
Mox20I TGGCCA 2 cut(s) 236, 661
MscI TGGCCA 2 cut(s) 236, 661
MseI TTAA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 536
Msp20I TGGCCA 2 cut(s) 236, 661
MspA1I CMGCKG 1 cut(s) 492
MspI CCGG 3 cut(s) 321, 965, 1161
MspR9I CCNGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
MssI GTTTAAAC 1 cut(s) 735
MunI CAATTG 1 cut(s) 516
MvaI CCWGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 4 cut(s) 342, 445, 853, 908
NdeII GATC 3 cut(s) 603, 622, 1075
NlaIII CATG 5 cut(s) 538, 604, 634, 860, 1076
NlaIV GGNNCC 3 cut(s) 6, 7, 19
NmeAIII GCCGAG 1 cut(s) 1008
PagI TCATGA 2 cut(s) 534, 1072
PfeI GAWTC 4 cut(s) 167, 326, 794, 1103
PflFI GACNNNGTC 1 cut(s) 683
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 20
PkrI GCNGC 9 cut(s) 58, 72, 338, 423, 494, 512, 599, 981, 1111
PleI GAGTC 3 cut(s) 535, 551, 868
PmeI GTTTAAAC 1 cut(s) 735
PpsI GAGTC 3 cut(s) 535, 551, 868
PpuMI RGGWCCY 2 cut(s) 5, 240
Psp5II RGGWCCY 2 cut(s) 5, 240
Psp6I CCWGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
PspN4I GGNNCC 3 cut(s) 6, 7, 19
PspPI GGNCC 3 cut(s) 5, 18, 240
PspPPI RGGWCCY 2 cut(s) 5, 240
PstI CTGCAG 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 683
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 536
SacII CCGCGG 1 cut(s) 493
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 9 cut(s) 57, 71, 337, 422, 493, 511, 598, 980, 1110
Sau3AI GATC 3 cut(s) 603, 622, 1075
Sau96I GGNCC 3 cut(s) 5, 18, 240
SchI GAGTC 3 cut(s) 535, 551, 869
ScrFI CCNGG 7 cut(s) 22, 77, 238, 388, 436, 663, 1080
SduI GDGCHC 2 cut(s) 756, 849
SfcI CTRYAG 3 cut(s) 549, 595, 651
Sfr303I CCGCGG 1 cut(s) 493
SgrBI CCGCGG 1 cut(s) 493
SinI GGWCC 3 cut(s) 5, 18, 240
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 9 cut(s) 65, 108, 161, 179, 502, 516, 942, 1095, 1166
SsiI CCGC 5 cut(s) 490, 492, 510, 877, 1066
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 7 cut(s) 20, 75, 236, 386, 434, 661, 1078
TaaI ACNGT 2 cut(s) 115, 373
TaqI TCGA 4 cut(s) 140, 260, 579, 1174
TasI AATT 9 cut(s) 65, 108, 161, 179, 502, 516, 942, 1095, 1166
TauI GCSGC 2 cut(s) 495, 513
TfiI GAWTC 4 cut(s) 167, 326, 794, 1103
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 1 cut(s) 130
TseI GCWGC 7 cut(s) 56, 70, 336, 421, 597, 979, 1109
TspDTI ATGAA 3 cut(s) 318, 374, 1065
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 1 cut(s) 130
Tth111I GACNNNGTC 1 cut(s) 683
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 3 cut(s) 5, 18, 240
XapI RAATTY 3 cut(s) 161, 179, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 1006
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.