pycom08g03860

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003789
Physical Location & Seq
Reverse (-)
2935760 .. 2940169
4410 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1917 bp
ATGCAATGCAAATACAAGGATAAAAGCGGGAGAAAATTACACGAACTAAAACAGTGTTTCATTTTTGGGTCAAAAGCTATAATTGAATTCCTCTGGTTTTCCTCCGTAAATTTCTGTCACCTTCTTCGTCTGTTTGCTGACTCTCTCACACACTACACTTCCCTCTCTCTCTCTCTCTCTCTCACTCCGATCCTCATTACCTTCACCTCCACCGATCACCGTCGTCTTCTTACAATGCCCGGTGACGAAAAATGGTGCGTGGTCACCGGAGGCCGAGGCTTCGCGGCGCGCCACTTGGTCCAGATGCTGATCCGATACGACGTGTTCCGGGTCCGAATCGCCGACCTGGGCTCCACCGTCGATCTAGAACCTTCCGAGGAGAACGGCATTCTTGCCGAAGCTCTGAGCTCCGGCCGCGCCCAGTACGTGTCCCTCGACCTTCGCAGCAAGAGCCAAGTTCTTCAAGCGTTTCAAGGTGCGGAGGTTGTGTTTCACATGGCGGCTCCCAATTCGTCCATCAACAATTACAAGCTCCACCACTCGGTCAATGTAGAAGGAACACAGAACGCGATTGATGCTTGTACTGAGCTGAAAGTAAAGAGACTAATCTATACTAGCTCCCCGAGTGTTGTTTTCGATGGGATTCATGGGATTGTTGACGGGGATGAGTCGTTGTCATATCCACCTAAGCATAATGATTTCTATTCGGCAACGAAAGCTCAGGGAGAAGAATTGGTGATCAAGGCAAATGGTGTTAAGGGGCTTCTTACATGTTGCATTCGCCCTAGCAGCATTTTTGGACCTGGTGATAGGTTGCTCGTTCCGTCTTTGGTAGTTGCTGCCAGGGCAGGGAAATCCAAGTACATCATTGGTGATGGCAACAACATTTATGATTTCACATATGTTGAAAATGTGGCACATGCGCATATATGTGCTGAGCGAGCTCTTGCATCAGAAGGGACAGTTGCAGATAAAGCGGCAGGGCAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTGTTTCACTTATTCTAGAAGGTCTTGGTTATGAAAGGCCAAGAATAAAAATCCCTGCATTTGTTGTGATGCCAATTGCACATATGGTGGAGTTGACATATAAGCTGTTAGGCCCATATGGGATGAAGGTTCCACAGTTGACCCCTTCGAGAATTAGACTCCTCTCTTGCACCCGATCTTTTAAATGTTCGAAGGCACAGGATCGAATTGGCTACACACCCATCGTATCGCTTCAGGAGGGTCTGAAAAGGACAATTGAGGCATACCCACATTTGAGGGCTGAACATCTACCGAAAAGAGGAGGGCCATCTAAAGCGTCAATATATCTTGGAAGCGGAAGGGTTGCTGACACGTTACTCTGGAAGGATAAAAAACAGACCTTCAAGGCGTTATTTGTTTTGATTGCAATCTACTACAACTTCGTTGTACCTGGATCTACAATTATTACTATGCTTTCAAAGGTTCTCCTGGTAGCTTCAGTATTCGTGTTCATTCATGCTGTTTTACCAGAAAAGATATTAGGATACAAAATTGAGAAGCTTCAGTCATCACATTTCCACTTATCAGAAGAGACGTCGCAACAAATTGCCAAGTCTGTGGCTTCATCATGGAATGCATCTGTTAAAACATTGAAATCTCTTGGCAAGGGAAATGATGGGATTTTGTTCCTTAAGGTTACTCTCACCCTACTGGTTCTTAGCTTCCTTGGAGCGTTTTCACTTCAGAACTTATATGTTATAGGAGTTCCCGCTGCCTTCATAGTTTTCTACGTGTATGAGAAGGAGGAGCAAAAAATTGACGACATGGTCCTCGAAGCTCTCTCTCGTGGATGCAAATTGAAATCGGATATTGTAAAAAAATTCTATACTTCCAAGAAGATTGAGTGA

Protein Analysis

639

Amino Acids

70.89

Weight (kDa)

9.21

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 87 - 361 1.5e-75 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 87 - 315 4.6e-33 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 87 - 207 2.7e-07 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 88 - 316 4.4e-15 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 135 - 211 8.5e-08 NAD(P)H-binding
RmlD_sub_bind PF04321 140 - 359 1.7e-07 RmlD substrate binding domain
NAD_binding_4 PF07993 154 - 307 1.1e-12 Male sterility protein
Reticulon PF02453 458 - 613 2.2e-32 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AasI GACNNNNNNGTC 1 cut(s) 1835
AatII GACGTC 1 cut(s) 1607
Acc16I TGCGCA 1 cut(s) 924
AccII CGCG 4 cut(s) 284, 289, 417, 569
AciI CCGC 8 cut(s) 27, 284, 415, 479, 500, 977, 1365, 1779
AclWI GGATC 4 cut(s) 184, 304, 1239, 1471
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 3 cut(s) 86, 109, 1889
AcuI CTGAAG 4 cut(s) 1247, 1491, 1556, 1736
AcyI GRCGYC 1 cut(s) 1604
AfaI GTAC 4 cut(s) 425, 583, 863, 1458
AfiI CCNNNNNNNGG 3 cut(s) 541, 849, 1328
AflII CTTAAG 1 cut(s) 1700
AflIII ACRYGT 5 cut(s) 321, 426, 770, 1380, 1800
AgsI TTSAA 8 cut(s) 86, 464, 473, 908, 1414, 1488, 1663, 1870
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 5 cut(s) 345, 802, 842, 1459, 1497
Alw21I GWGCWC 2 cut(s) 410, 946
Alw26I GTCTC 2 cut(s) 595, 1595
AlwI GGATC 4 cut(s) 184, 304, 1239, 1471
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 5 cut(s) 271, 412, 1067, 1141, 1334
ApeKI GCWGC 4 cut(s) 444, 789, 839, 1781
ApoI RAATTY 3 cut(s) 86, 109, 1889
AscI GGCGCGCC 1 cut(s) 287
AspLEI GCGC 4 cut(s) 289, 291, 419, 925
AspS9I GGNCC 6 cut(s) 298, 331, 800, 1142, 1334, 1837
AsuC2I CCSGG 2 cut(s) 240, 329
AsuHPI GGTGA 9 cut(s) 110, 196, 209, 254, 256, 748, 818, 884, 1705
AsuII TTCGAA 1 cut(s) 1220
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 4 cut(s) 298, 331, 800, 1837
BanII GRGCYC 3 cut(s) 353, 410, 946
BauI CACGAG 1 cut(s) 1854
BbsI GAAGAC 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 410, 946
BbvI GCAGC 4 cut(s) 456, 801, 826, 1768
BccI CCATC 6 cut(s) 524, 632, 869, 1259, 1345, 1679
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 5 cut(s) 347, 804, 844, 1461, 1499
BciVI GTATCC 1 cut(s) 1547
BclI TGATCA 1 cut(s) 738
BcnI CCSGG 2 cut(s) 240, 329
BcoDI GTCTC 2 cut(s) 595, 1595
BfaI CTAG 4 cut(s) 365, 615, 786, 1046
BfrI CTTAAG 1 cut(s) 1700
BfuI GTATCC 1 cut(s) 1547
BisI GCNGC 8 cut(s) 285, 415, 445, 501, 790, 840, 978, 1782
BlpI GCTNAGC 1 cut(s) 936
BlsI GCNGC 8 cut(s) 286, 416, 446, 502, 791, 841, 979, 1783
Bme1390I CCNGG 7 cut(s) 240, 329, 347, 804, 844, 1461, 1499
Bme18I GGWCC 4 cut(s) 298, 331, 800, 1837
BmeT110I CYCGRG 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 6 cut(s) 298, 331, 800, 1142, 1334, 1837
BmiI GGNNCC 5 cut(s) 332, 352, 504, 1011, 1161
BmrFI CCNGG 7 cut(s) 240, 329, 347, 804, 844, 1461, 1499
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 6 cut(s) 294, 565, 959, 1089, 1655, 1850
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 218
Bpu10I CCTNAGC 2 cut(s) 687, 720
Bpu1102I GCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 1220
BpuMI CCSGG 2 cut(s) 240, 329
BsaAI YACGTR 2 cut(s) 427, 1801
BsaBI GATNNNNATC 2 cut(s) 308, 1436
BsaHI GRCGYC 1 cut(s) 1604
BsaJI CCNNGG 5 cut(s) 274, 346, 375, 843, 1735
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 473, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 541, 849, 1328
Bse1I ACTGG 2 cut(s) 421, 1725
Bse3DI GCAATG 1 cut(s) 11
Bse8I GATNNNNATC 2 cut(s) 308, 1436
BseBI CCWGG 5 cut(s) 347, 804, 844, 1461, 1499
BseDI CCNNGG 5 cut(s) 274, 346, 375, 843, 1735
BseGI GGATG 3 cut(s) 670, 1158, 1865
BseJI GATNNNNATC 2 cut(s) 308, 1436
BseLI CCNNNNNNNGG 3 cut(s) 541, 849, 1328
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 4 cut(s) 395, 576, 734, 927
BseNI ACTGG 2 cut(s) 421, 1725
BsePI GCGCGC 1 cut(s) 287
BseRI GAGGAG 4 cut(s) 392, 1181, 1344, 1829
BseX3I CGGCCG 1 cut(s) 412
BseXI GCAGC 4 cut(s) 456, 801, 826, 1768
Bsh1236I CGCG 4 cut(s) 284, 289, 417, 569
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 5 cut(s) 273, 414, 1069, 1143, 1336
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 2 cut(s) 410, 946
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 4 cut(s) 240, 267, 328, 411
BslFI GGGAC 2 cut(s) 415, 973
BslI CCNNNNNNNGG 3 cut(s) 541, 849, 1328
BsmAI GTCTC 2 cut(s) 595, 1595
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 2 cut(s) 415, 973
BsmI GAATGC 3 cut(s) 387, 777, 1648
BsnI GGCC 5 cut(s) 273, 414, 1069, 1143, 1336
BsoBI CYCGRG 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 1220
Bsp1286I GDGCHC 3 cut(s) 353, 410, 946
Bsp143I GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
Bsp1720I GCTNAGC 1 cut(s) 936
BspACI CCGC 8 cut(s) 27, 284, 415, 479, 500, 977, 1365, 1779
BspANI GGCC 5 cut(s) 273, 414, 1069, 1143, 1336
BspCNI CTCAG 4 cut(s) 396, 577, 733, 928
BspFNI CGCG 4 cut(s) 284, 289, 417, 569
BspLI GGNNCC 5 cut(s) 332, 352, 504, 1011, 1161
BspPI GGATC 4 cut(s) 184, 304, 1239, 1471
BspT104I TTCGAA 1 cut(s) 1220
BspTI CTTAAG 1 cut(s) 1700
BsrDI GCAATG 1 cut(s) 11
BsrI ACTGG 2 cut(s) 421, 1725
BssECI CCNNGG 5 cut(s) 274, 346, 375, 843, 1735
BssHII GCGCGC 1 cut(s) 287
BssMI GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
BssNI GRCGYC 1 cut(s) 1604
BssSI CACGAG 1 cut(s) 1854
BssT1I CCWWGG 1 cut(s) 1735
Bst2BI CACGAG 1 cut(s) 1854
Bst2UI CCWGG 5 cut(s) 347, 804, 844, 1461, 1499
Bst4CI ACNGT 5 cut(s) 54, 221, 358, 964, 1167
Bst6I CTCTTC 1 cut(s) 1593
BstACI GRCGYC 1 cut(s) 1604
BstAFI CTTAAG 1 cut(s) 1700
BstBAI YACGTR 2 cut(s) 427, 1801
BstBI TTCGAA 1 cut(s) 1220
BstC8I GCNNGC 3 cut(s) 289, 942, 987
BstDEI CTNAG 6 cut(s) 404, 585, 687, 720, 936, 1727
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 3 cut(s) 670, 1158, 1865
BstFNI CGCG 4 cut(s) 284, 289, 417, 569
BstHHI GCGC 4 cut(s) 289, 291, 419, 925
BstKTI GATC 8 cut(s) 192, 217, 312, 364, 741, 1208, 1234, 1466
BstMAI GTCTC 2 cut(s) 595, 1595
BstMBI GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 9 cut(s) 414, 450, 575, 716, 789, 845, 941, 974, 986
BstNI CCWGG 5 cut(s) 347, 804, 844, 1461, 1499
BstNSI RCATGY 2 cut(s) 774, 923
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 7 cut(s) 238, 327, 345, 802, 842, 1459, 1497
BstUI CGCG 4 cut(s) 284, 289, 417, 569
BstV1I GCAGC 4 cut(s) 456, 801, 826, 1768
BstV2I GAAGAC 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 1463
BstXI CCANNNNNNTGG 1 cut(s) 1627
BstYI RGATCY 1 cut(s) 1463
BstZI CGGCCG 1 cut(s) 412
BsuI GTATCC 1 cut(s) 1547
BsuRI GGCC 5 cut(s) 273, 414, 1069, 1143, 1336
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 3 cut(s) 670, 1158, 1865
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 3 cut(s) 289, 942, 987
CaiI CAGNNNCTG 1 cut(s) 307
CfoI GCGC 4 cut(s) 289, 291, 419, 925
Cfr13I GGNCC 6 cut(s) 298, 331, 800, 1142, 1334, 1837
CseI GACGC 1 cut(s) 1335
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 4 cut(s) 424, 582, 862, 1457
CviAII CATG 7 cut(s) 496, 647, 771, 920, 1526, 1638, 1834
CviQI GTAC 4 cut(s) 424, 582, 862, 1457
DdeI CTNAG 6 cut(s) 404, 585, 687, 720, 936, 1727
DpnI GATC 8 cut(s) 191, 216, 311, 363, 740, 1207, 1233, 1465
DpnII GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
DraI TTTAAA 1 cut(s) 1213
DrdI GACNNNNNNGTC 1 cut(s) 1835
DseDI GACNNNNNNGTC 1 cut(s) 1835
EaeI YGGCCR 1 cut(s) 412
EagI CGGCCG 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 1593
EarI CTCTTC 1 cut(s) 1593
Ecl136II GAGCTC 2 cut(s) 408, 944
EclXI CGGCCG 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 1735
Eco24I GRGCYC 3 cut(s) 353, 410, 946
Eco47I GGWCC 4 cut(s) 298, 331, 800, 1837
Eco52I CGGCCG 1 cut(s) 412
Eco53kI GAGCTC 2 cut(s) 408, 944
Eco57I CTGAAG 4 cut(s) 1247, 1491, 1556, 1736
Eco88I CYCGRG 1 cut(s) 622
Eco91I GGTNACC 1 cut(s) 262
EcoICRI GAGCTC 2 cut(s) 408, 944
EcoO65I GGTNACC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 5 cut(s) 345, 802, 842, 1459, 1497
EcoT14I CCWWGG 1 cut(s) 1735
EcoT22I ATGCAT 1 cut(s) 1648
EcoT38I GRGCYC 3 cut(s) 353, 410, 946
ErhI CCWWGG 1 cut(s) 1735
Esp3I CGTCTC 1 cut(s) 1595
FaeI CATG 7 cut(s) 499, 650, 774, 923, 1529, 1641, 1837
FaqI GGGAC 2 cut(s) 415, 973
FatI CATG 7 cut(s) 495, 646, 770, 919, 1525, 1637, 1833
FauI CCCGC 2 cut(s) 20, 1786
FauNDI CATATG 3 cut(s) 901, 1113, 1147
FbaI TGATCA 1 cut(s) 738
Fnu4HI GCNGC 8 cut(s) 285, 415, 445, 501, 790, 840, 978, 1782
FokI GGATG 3 cut(s) 677, 1165, 1872
FriOI GRGCYC 3 cut(s) 353, 410, 946
Fsp4HI GCNGC 8 cut(s) 285, 415, 445, 501, 790, 840, 978, 1782
FspAI RTGCGCAY 1 cut(s) 924
FspBI CTAG 4 cut(s) 365, 615, 786, 1046
FspI TGCGCA 1 cut(s) 924
GlaI GCGC 4 cut(s) 288, 290, 418, 924
GluI GCNGC 8 cut(s) 285, 415, 445, 501, 790, 840, 978, 1782
HaeIII GGCC 5 cut(s) 273, 414, 1069, 1143, 1336
HapII CCGG 4 cut(s) 240, 267, 328, 411
HgaI GACGC 1 cut(s) 1335
HhaI GCGC 4 cut(s) 289, 291, 419, 925
Hin1I GRCGYC 1 cut(s) 1604
Hin1II CATG 7 cut(s) 499, 650, 774, 923, 1529, 1641, 1837
Hin6I GCGC 4 cut(s) 287, 289, 417, 923
HinP1I GCGC 4 cut(s) 287, 289, 417, 923
HincII GTYRAC 3 cut(s) 658, 1125, 1170
HindII GTYRAC 3 cut(s) 658, 1125, 1170
HindIII AAGCTT 1 cut(s) 1568
HinfI GANTC 5 cut(s) 140, 336, 643, 668, 1188
HpaII CCGG 4 cut(s) 240, 267, 328, 411
HphI GGTGA 9 cut(s) 110, 196, 209, 254, 256, 748, 818, 884, 1705
Hpy166II GTNNAC 3 cut(s) 658, 1125, 1170
Hpy188III TCNNGA 6 cut(s) 301, 365, 1046, 1179, 1265, 1390
Hpy8I GTNNAC 3 cut(s) 658, 1125, 1170
Hpy99I CGWCG 4 cut(s) 225, 323, 362, 1609
HpyCH4III ACNGT 5 cut(s) 54, 221, 358, 964, 1167
HpyCH4IV ACGT 5 cut(s) 321, 426, 1382, 1604, 1800
HpyF10VI GCNNNNNNNGC 9 cut(s) 414, 450, 575, 716, 789, 845, 941, 974, 986
HpyF3I CTNAG 6 cut(s) 404, 585, 687, 720, 936, 1727
HpySE526I ACGT 5 cut(s) 321, 426, 1382, 1604, 1800
Hsp92I GRCGYC 1 cut(s) 1604
Hsp92II CATG 7 cut(s) 499, 650, 774, 923, 1529, 1641, 1837
HspAI GCGC 4 cut(s) 287, 289, 417, 923
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
LmnI GCTCC 7 cut(s) 356, 413, 508, 537, 623, 1739, 1816
Lsp1109I GCAGC 4 cut(s) 456, 801, 826, 1768
LweI GCATC 6 cut(s) 294, 565, 959, 1089, 1655, 1850
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 4 cut(s) 365, 615, 786, 1046
MaeII ACGT 5 cut(s) 321, 426, 1382, 1604, 1800
MaeIII GTNAC 5 cut(s) 116, 242, 262, 1383, 1705
MalI GATC 8 cut(s) 191, 216, 311, 363, 740, 1207, 1233, 1465
MboI GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
MboII GAAGA 5 cut(s) 116, 218, 452, 740, 1610
MfeI CAATTG 2 cut(s) 1104, 1284
MflI RGATCY 1 cut(s) 1463
MhlI GDGCHC 3 cut(s) 353, 410, 946
MlyI GAGTC 3 cut(s) 134, 677, 1182
Mph1103I ATGCAT 1 cut(s) 1648
MseI TTAA 4 cut(s) 756, 1212, 1653, 1701
MslI CAYNNNNRTG 1 cut(s) 930
MspA1I CMGCKG 1 cut(s) 1781
MspCI CTTAAG 1 cut(s) 1700
MspI CCGG 4 cut(s) 240, 267, 328, 411
MspR9I CCNGG 7 cut(s) 240, 329, 347, 804, 844, 1461, 1499
MunI CAATTG 2 cut(s) 1104, 1284
Mva1269I GAATGC 3 cut(s) 387, 777, 1648
MvaI CCWGG 5 cut(s) 347, 804, 844, 1461, 1499
MvnI CGCG 4 cut(s) 284, 289, 417, 569
MwoI GCNNNNNNNGC 9 cut(s) 414, 450, 575, 716, 789, 845, 941, 974, 986
NciI CCSGG 2 cut(s) 240, 329
NdeI CATATG 3 cut(s) 901, 1113, 1147
NdeII GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
NlaIII CATG 7 cut(s) 499, 650, 774, 923, 1529, 1641, 1837
NlaIV GGNNCC 5 cut(s) 332, 352, 504, 1011, 1161
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 3 cut(s) 116, 242, 262
NsbI TGCGCA 1 cut(s) 924
NsiI ATGCAT 1 cut(s) 1648
NspI RCATGY 2 cut(s) 774, 923
NspV TTCGAA 1 cut(s) 1220
PalAI GGCGCGCC 1 cut(s) 287
PauI GCGCGC 1 cut(s) 287
PciI ACATGT 1 cut(s) 770
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PctI GAATGC 3 cut(s) 387, 777, 1648
PfeI GAWTC 2 cut(s) 336, 643
PflFI GACNNNGTC 1 cut(s) 1835
PkrI GCNGC 8 cut(s) 286, 416, 446, 502, 791, 841, 979, 1783
PleI GAGTC 3 cut(s) 134, 676, 1182
PpsI GAGTC 3 cut(s) 134, 676, 1182
Ppu21I YACGTR 2 cut(s) 427, 1801
PscI ACATGT 1 cut(s) 770
PsiI TTATAA 1 cut(s) 998
Psp124BI GAGCTC 2 cut(s) 410, 946
Psp6I CCWGG 5 cut(s) 345, 802, 842, 1459, 1497
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 5 cut(s) 345, 802, 842, 1459, 1497
PspN4I GGNNCC 5 cut(s) 332, 352, 504, 1011, 1161
PspPI GGNCC 6 cut(s) 298, 331, 800, 1142, 1334, 1837
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 1463
PsyI GACNNNGTC 1 cut(s) 1835
PteI GCGCGC 1 cut(s) 287
RsaI GTAC 4 cut(s) 425, 583, 863, 1458
RsaNI GTAC 4 cut(s) 424, 582, 862, 1457
RseI CAYNNNNRTG 1 cut(s) 930
SacI GAGCTC 2 cut(s) 410, 946
SaqAI TTAA 4 cut(s) 756, 1212, 1653, 1701
SatI GCNGC 8 cut(s) 285, 415, 445, 501, 790, 840, 978, 1782
Sau3AI GATC 8 cut(s) 189, 214, 309, 361, 738, 1205, 1231, 1463
Sau96I GGNCC 6 cut(s) 298, 331, 800, 1142, 1334, 1837
SchI GAGTC 3 cut(s) 134, 677, 1182
ScrFI CCNGG 7 cut(s) 240, 329, 347, 804, 844, 1461, 1499
SduI GDGCHC 3 cut(s) 353, 410, 946
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 6 cut(s) 294, 565, 959, 1089, 1655, 1850
SfuI TTCGAA 1 cut(s) 1220
SgsI GGCGCGCC 1 cut(s) 287
SinI GGWCC 4 cut(s) 298, 331, 800, 1837
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 1700
SmoI CTYRAG 1 cut(s) 1700
SsiI CCGC 8 cut(s) 27, 284, 415, 479, 500, 977, 1365, 1779
SspMI CTAG 4 cut(s) 365, 615, 786, 1046
SstI GAGCTC 2 cut(s) 410, 946
StyD4I CCNGG 7 cut(s) 238, 327, 345, 802, 842, 1459, 1497
StyI CCWWGG 1 cut(s) 1735
TaaI ACNGT 5 cut(s) 54, 221, 358, 964, 1167
TaiI ACGT 5 cut(s) 324, 429, 1385, 1607, 1803
TaqI TCGA 7 cut(s) 360, 435, 636, 1178, 1220, 1234, 1842
TaqII GACCGA 1 cut(s) 532
TatI WGTACW 2 cut(s) 581, 861
TauI GCSGC 4 cut(s) 287, 417, 503, 980
TfiI GAWTC 2 cut(s) 336, 643
Tru1I TTAA 4 cut(s) 756, 1212, 1653, 1701
Tru9I TTAA 4 cut(s) 756, 1212, 1653, 1701
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 3 cut(s) 116, 242, 262
TseI GCWGC 4 cut(s) 444, 789, 839, 1781
Tsp45I GTSAC 3 cut(s) 116, 242, 262
TspDTI ATGAA 8 cut(s) 49, 635, 1077, 1169, 1510, 1514, 1623, 1777
TspGWI ACGGA 2 cut(s) 94, 813
TspRI CASTG 1 cut(s) 59
Tth111I GACNNNGTC 1 cut(s) 1835
Vha464I CTTAAG 1 cut(s) 1700
VpaK11BI GGWCC 4 cut(s) 298, 331, 800, 1837
XapI RAATTY 3 cut(s) 86, 109, 1889
XbaI TCTAGA 2 cut(s) 364, 1045
XceI RCATGY 2 cut(s) 774, 923
XspI CTAG 4 cut(s) 365, 615, 786, 1046
ZraI GACGTC 1 cut(s) 1605
Zsp2I ATGCAT 1 cut(s) 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.