pycom08g13100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005741
Physical Location & Seq
Forward (+)
11743825 .. 11744556
732 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13100.1

Sequence Viewer

Length: 732 bp
ATGAAAAGAAATACAATATTCACTCTTTATTCAGAAGCTCCAATTATAAGTCAAAACAATGTTTCAAAACTTAAAGAAAAAGGGAGTGAAAACCACACCGATACATTGTATGAAGCCAAAAGATCATACAACTACTTATTAATGCTTTTTGCTGCAACAGGCAATATGAATTTAGTTGTAGGAGTCTTGGTTCTCCAAGAGGTAGTTCTCAATCAACTTGAAGAGGTGGGAGGCCTTCTCTTTGCCAGCCTTAACATGCTCTTCCTTGATCTCAACGTCACCTTTTGTGTGATAGTGGCTTATGCTCTTGATGACGGAACCGCTGCCGGAAGCCACCAACTTGGTCTCATAAGAGATCTTCTCAATTGTCTCAGTGATAGCATCTCCTTCGATCACACTGTACTTGTAAACAAAGTTGTCCTTGTCAACCCCATCAATTCTGTGCTTCACATAGCTGTATGTGCTACCTTCACCGAAGTTGATCTTCTTAATGGTTCCAACTCCGCCATCTCCTTCAAGGATCTCAGTGCTCTTCACTGCCTGTGGTGCAATCTTGGGGATGAGGTTGTCAGCATCAAGAACAAAAGCATTGTACAACCTAGCAGGGGGGATGACAGAGGTGAACTCGAATTCGTATGTGAAAACACCCATGATTCTGAGAAGTGTTTGGAAAGTAAGAAAATAGATATTGATGAAGAGGAAGATGATGTAATGGGAGGTTTTGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.78

Weight (kDa)

4.55

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AciI CCGC 2 cut(s) 321, 504
AclWI GGATC 1 cut(s) 528
AcsI RAATTY 2 cut(s) 169, 629
AfaI GTAC 2 cut(s) 402, 594
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 3 cut(s) 66, 221, 517
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AloI GAACNNNNNNTCC 2 cut(s) 174, 206
AluBI AGCT 2 cut(s) 38, 455
AluI AGCT 2 cut(s) 38, 455
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 2 cut(s) 350, 374
AlwI GGATC 1 cut(s) 528
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 2 cut(s) 152, 323
ApoI RAATTY 2 cut(s) 169, 629
AseI ATTAAT 1 cut(s) 140
AsuHPI GGTGA 3 cut(s) 271, 463, 632
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 139, 310
BccI CCATC 2 cut(s) 440, 515
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BcoDI GTCTC 2 cut(s) 350, 374
BfaI CTAG 1 cut(s) 600
BglII AGATCT 1 cut(s) 355
BisI GCNGC 2 cut(s) 153, 324
BlsI GCNGC 2 cut(s) 154, 325
BmiI GGNNCC 2 cut(s) 319, 496
BmsI GCATC 2 cut(s) 390, 582
BplI GAGNNNNNCTC 6 cut(s) 222, 254, 345, 377, 609, 641
BsaI GGTCTC 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 605
BseGI GGATG 2 cut(s) 565, 616
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 3 cut(s) 385, 538, 648
BseXI GCAGC 2 cut(s) 139, 310
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 2 cut(s) 350, 374
BsnI GGCC 1 cut(s) 234
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 532
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 6 cut(s) 122, 268, 355, 391, 481, 520
BspACI CCGC 2 cut(s) 321, 504
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 3 cut(s) 384, 537, 649
BspLI GGNNCC 2 cut(s) 319, 496
BspPI GGATC 1 cut(s) 528
BspQI GCTCTTC 2 cut(s) 266, 537
BspTNI GGTCTC 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 592
BssMI GATC 6 cut(s) 122, 268, 355, 391, 481, 520
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 4 cut(s) 216, 266, 537, 690
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 3 cut(s) 371, 524, 657
BstF5I GGATG 2 cut(s) 565, 616
BstKTI GATC 6 cut(s) 125, 271, 358, 394, 484, 523
BstMAI GTCTC 2 cut(s) 350, 374
BstMBI GATC 6 cut(s) 122, 268, 355, 391, 481, 520
BstMWI GCNNNNNNNGC 2 cut(s) 461, 546
BstNSI RCATGY 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 139, 310
BstX2I RGATCY 2 cut(s) 355, 520
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 2 cut(s) 355, 520
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 2 cut(s) 565, 616
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 4 cut(s) 379, 396, 532, 535
Cac8I GCNNGC 1 cut(s) 247
Csp6I GTAC 2 cut(s) 401, 593
CviAII CATG 2 cut(s) 256, 650
CviJI RGCY 7 cut(s) 38, 116, 234, 249, 299, 333, 455
CviKI_1 RGCY 7 cut(s) 38, 116, 234, 249, 299, 333, 455
CviQI GTAC 2 cut(s) 401, 593
DdeI CTNAG 3 cut(s) 371, 524, 657
DpnI GATC 6 cut(s) 124, 270, 357, 393, 483, 522
DpnII GATC 6 cut(s) 122, 268, 355, 391, 481, 520
Eam1104I CTCTTC 4 cut(s) 216, 266, 537, 690
EarI CTCTTC 4 cut(s) 216, 266, 537, 690
EciI GGCGGA 1 cut(s) 493
Eco147I AGGCCT 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 629
FaeI CATG 2 cut(s) 259, 653
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 468, 500
FatI CATG 2 cut(s) 255, 649
Fnu4HI GCNGC 2 cut(s) 153, 324
FokI GGATG 2 cut(s) 572, 623
Fsp4HI GCNGC 2 cut(s) 153, 324
FspBI CTAG 1 cut(s) 600
GluI GCNGC 2 cut(s) 153, 324
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 327
Hin1II CATG 2 cut(s) 259, 653
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 2 cut(s) 183, 653
HpaII CCGG 1 cut(s) 327
HphI GGTGA 3 cut(s) 271, 463, 632
Hpy166II GTNNAC 3 cut(s) 409, 427, 623
Hpy188I TCNGA 2 cut(s) 34, 658
Hpy188III TCNNGA 2 cut(s) 308, 577
Hpy8I GTNNAC 3 cut(s) 409, 427, 623
HpyAV CCTTC 4 cut(s) 245, 397, 478, 523
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 155, 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 546
HpyF3I CTNAG 3 cut(s) 371, 524, 657
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 259, 653
Kzo9I GATC 6 cut(s) 122, 268, 355, 391, 481, 520
LguI GCTCTTC 2 cut(s) 266, 537
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 5 cut(s) 144, 259, 340, 554, 589
Lsp1109I GCAGC 2 cut(s) 139, 310
LweI GCATC 2 cut(s) 390, 582
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 277
MalI GATC 6 cut(s) 124, 270, 357, 393, 483, 522
MboI GATC 6 cut(s) 122, 268, 355, 391, 481, 520
MboII GAAGA 7 cut(s) 233, 253, 350, 476, 524, 707, 713
MfeI CAATTG 1 cut(s) 364
MflI RGATCY 2 cut(s) 355, 520
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 5 cut(s) 42, 169, 364, 436, 629
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 7 cut(s) 193, 217, 224, 556, 611, 691, 710
MseI TTAA 4 cut(s) 72, 140, 252, 489
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 327
MunI CAATTG 1 cut(s) 364
MwoI GCNNNNNNNGC 2 cut(s) 461, 546
NdeII GATC 6 cut(s) 122, 268, 355, 391, 481, 520
NlaIII CATG 2 cut(s) 259, 653
NlaIV GGNNCC 2 cut(s) 319, 496
NmuCI GTSAC 1 cut(s) 277
NspI RCATGY 1 cut(s) 259
PceI AGGCCT 1 cut(s) 234
PciSI GCTCTTC 2 cut(s) 266, 537
PfeI GAWTC 1 cut(s) 653
PkrI GCNGC 2 cut(s) 154, 325
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 140
PsiI TTATAA 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 319, 496
PsuI RGATCY 2 cut(s) 355, 520
RsaI GTAC 2 cut(s) 402, 594
RsaNI GTAC 2 cut(s) 401, 593
SapI GCTCTTC 2 cut(s) 266, 537
SaqAI TTAA 4 cut(s) 72, 140, 252, 489
SatI GCNGC 2 cut(s) 153, 324
Sau3AI GATC 6 cut(s) 122, 268, 355, 391, 481, 520
SchI GAGTC 1 cut(s) 192
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 2 cut(s) 390, 582
Sse9I AATT 5 cut(s) 42, 169, 364, 436, 629
SseBI AGGCCT 1 cut(s) 234
SsiI CCGC 2 cut(s) 321, 504
SspI AATATT 1 cut(s) 18
SspMI CTAG 1 cut(s) 600
StuI AGGCCT 1 cut(s) 234
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 390, 627
TasI AATT 5 cut(s) 42, 169, 364, 436, 629
TatI WGTACW 2 cut(s) 400, 592
TfiI GAWTC 1 cut(s) 653
Tru1I TTAA 4 cut(s) 72, 140, 252, 489
Tru9I TTAA 4 cut(s) 72, 140, 252, 489
TscAI CASTG 4 cut(s) 379, 403, 532, 542
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 152, 323
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 17, 126, 182, 708
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 4 cut(s) 379, 403, 532, 542
VspI ATTAAT 1 cut(s) 140
XapI RAATTY 2 cut(s) 169, 629
XceI RCATGY 1 cut(s) 259
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.