Rorug04G0193100

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002268
Physical Location & Seq
Reverse (-)
33635414 .. 33647594
12181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0193100.1

Sequence Viewer

Length: 4293 bp
ATGGCTGGAAAATCAAACAAAGGGAGGAACCGGAGAGGGTCACATAATGCTGCAAGTTCGTCTGATACGGTGGTCCAGTCTGATGCTCCTGTGAAAGACAACTCAAAAGATGAGTCGAGTGCTTCAGAGTCCGTCAAGGCCAATGCTAATGGTGTTCCAACTGTGCAAGAGTCAACTAGTGACAAGCTGGAGGTAAAGGATTCTGAAACTAATGTTGCAGTCGTCGAACCAAAGCAAGGTGAATCTGAAACAGAAACAGAAAATTCAGCTGGCCAACCTAAGCAAGGTGACCTTCATCTTTACCCTGTCTCTGTCAAGACACAAAGTGGCGAGAAGCTAGAACTACAATTGAACCCAGGAGATTCTGTTATGGATATAAGACAGTTTCTGCTTGATGCTCCTGAGACATGCTTCTTTACATGCTATGACTTGTTGCTACTCACCAAAGATGGCTCAACCTATCATTTGGAAGATTTTAATGAAATCTCTGAAGTTGCTGACATTACTATTGGTGGATGCTCCTTGGAAATGGTTCCAGCTTTATATGATGATAGATCTATAAGGGCTCATGTTCATCGTACCAGAGAATTACTTTCACTTTCCACACTTCATGCGTCACTATCAACATCGCTTGCATTACAATATGAGACAGCTCAGAACAAAGTAGCAACTCCTGGAGATACCATCAAAACTGAGGTCCCTGAGCTTGATGGTTTGGGTTTTATGGAGGATGTTGCCGGTGCATTGAGTAATTTGCTCTCATCACCCTCAAAAGAGATCAAATGCGTAGAGAGTATTGTGTTTTCATCCTTCAATCCTCCACCAAGCTATAGAAGGCTGGTCGGAGATTTGATTTATCTGGATGTCGTAACGTTGGAGGGTAATAAGCTTTGTATTACGGGGACTACAAAAATGTTTTATGTCAATTCAAGCGCCATAAATAATACCCTTAATCCCAGGCCAAGCAAAAGTTATGCAGAAGCGACCACACTTGTCGGGATTTTGCAAAAGATCAGCCCTAAGTTCCAAAAAGCTTTTCGTGAAATTTTGGAGCGGAGAGCGTCTGCACATCCTTTTGAAAACGTGCAATCTCTGTTACCACCAAATTCATGGTTAGGGTTACATCCAGTTCCAGATCACAAACGTGATGCAGCCAGAGCAGAGGATGCACTGACCCTCTCTTATGGTAGTGAGCTAATTGGCATGCAAAGGGATTGGAATGAGGAGTTGCAATCTTGTAGGGAATTTCCACATACAACTCCTCAGGAAAGAATTTTGCGGGATAGGGCTCTCTACAAAGTGACATCAGACTTCGTGGATGCAGCAATCAGTGGTGCTGTTGGAGTTATCAGCAGATGCATACCACCTATAAATCCAACTGATCCCGAGTGCTTCCACATGTATGTTCACAATAACATCTTCTTCAGTTTCGCTGTTGATGCGGATCTTGAGCAGCTGTCCAAGAACCATATGTCAGTTTCCAATTCGAAAATGGGGAGCACCGGCTCTTTAGGCAGTTCTTCTGAAAAGTCTACTGGCAGTTTGTTGCATAGAGAGAGTGAAATCCCCAATGGGGAAAAATGTGACGCGTCATGCGCAGGGGAATGCCACGATGCCATGGAATCAACTGCTGAGACACAATTGGGTGAGACTGAGCAGGCTACGTATGCTTCTGCAAATAATGATTTGAAAGGCACCAAGGCATACCAGGAAGCTGATGTCCCTGGACTTTATAATCTTGCTATGGCCATAATTGATTACAGAGGCCATAGAGTAGTAGCTCAGAGTGTTTTGCCAGGCATTCTTCAAGGGGATAAATCAGACTCTCTTTTATATGGTTCTGTTGATAATGGGAAAAAGATATGCTGGAATGAGGATTTTCATTCCAAGGTGGTGGAGGCTGCTAAACGCCTTCATTTGAAAGAACACACAGTCCTTGATGGATCTGGAAATGTTTTCAAGTTAGCTGCACCAGTGGAATGCAAGGGTATTGTTGGTAGTGATGACAGGCATTATCTTCTTGACTTGATGAGAGTAACTCCTCGTGATGCAAACTTCACTGGATCTGGGTCTCGATTTTGTATCTTGAGACCAGAACTAATTACTTCCTATTGCCAGGTCCTTGATGCAGAGAAATCAAAATCTAAGTCTAAATGTGAAGGAGAGGCTCAGGTCACCGCTGATGGGCCAAACGATAACCAGGACATCACAGAAAAAGAGAAGACCATTGATGCACAAGAAATTGTCTCACCACCAGCTGAAAGCTCTGAATCACGTGAGGAAACTCTTTTCAACCCTAATGTCTTCACTGAGTTTAAGTTGGCTGGAAATGCAGAGGAGATAGCAACAGATGAAGAAAATGTGAGGAAAGCCAGTTTGTATCTTACTGAGGTTGTTCTTCCAAAGTTCATACAAGATCTTTGCACACTTGAAGTATCACCCATGGATGGGCAGACACTAACTGAAGCACTCCATGCTCATGGAATTAATGTTCGTTATATTGGAAAAGTGGCTGATGGGACCAGGCATTTACCTCATCTTTGGGATCTTTGTTCTAATGAGATTGTGGTTAGATCAGCCAAGCACATTCTTAAGGATGTCTTAAGAGATACGGAGGATCATGAAATTGGGCCTGCAATCTGCCATTTCTTCAACTGTTTTTTCGGAAGCTATCAAGCTGTTGGCTCAAAAGTCGCTGCTAATAGTTCTCAGTCTAGAACCCCCAAAAAAGAACAAGCAGGTCATCAATCTCCGGGAAAACGGTCAAAGGGACAAGGAAGATGGAAGGCTGGAGCTTCTACAAAGAAGAGTGTATCTTCATATATGCATGTTAGCTCAGAGACTCTATGGTCTGACATTCAAGAATTTGCCAAACTCAAATATGAGTTTGAGTTGCCAAAGGATGCTAGGATGCATGTAAAGAAAGATTCGGTTATCCGTAATCTTTGCCAAAAGGTGGGCATTACAATTGCAGCTCGCAGATATGATCTGAAATCTGCAGCACCTTTTGAAATATCAGATATCTTGAATCTTCAACCTGTAGTGAAACACTCTGTTCCTGTTTGTTCAGAAGCTAAGGATCTTGTGGAAACAGGGAAAATCCAATTGGCTGAGGGAATGTTGAGTGAAGCCTACACTTTATTTTCCGAGGCCTTTTCCATACTTCAGCAGGTTACAGGCCCAATGCATCGGGAGGTTGCTAACTGCTGTCGGTATCTTGCAATGGTTCTATATCATGCTGGAGACATGGCGGGAGCCATAATGCAACAGCACAAGGAGCTAATTATAAATGAGCGTTGCCTGGGTTTGGACCACCCTGACACTGCTCACAGCTATGGGAATATGGCTCTTTTCTATCATGGACTTAACCAAACAGAACTCGCCTTACGACATATGTCCCGGGCGTTGCTCCTGTTAAGTTTGTCATCTGGCCCAGATCATCCTGATGTCGCAGCAACATTCATTAATGTTGCTATGATGTACCAGGATCTTGGGAAGATGGACACTGCTCTTCGATATTTGCAAGAGGCTTTGAAAAAGAATGAAAGGCTTCTGGGCGAGGAACATATCCAAACTGCTGTATGCTATCATGCACTTGCTATTGCATTTAATTGTATGGGCGCCTTCAAGCTCTCCCACCAGCATGAGAAGAAAACTTATGATATACTTGTCAAACAACTGGGTGAGGAGGATTCGAGGACACGTGACTCTCAAAACTGGATGAAGACATTTAAGTTGCGCGAGCAACAGATGAATGCACAAAAGCAAAAAGGTCAAGCTTTGAATGCCGCTTCAGCTCAAAAGGCAATCGACATGTTAAAGGCCAATCCTGACTTGATGCAGGCATTCCAATCTGCTGCTATAGCCGGCGGGTCTGGGAGTTCCAACACTTCTGTCAACAAATCTCTTAATGCTGCTGCAATAATGGGTGAAACTCTTCCCCGAGGGAGGGGAGTTGATGAGAGGGCTGCACGAGCAACAGCCGAAATCAGGAAGAAGGCAGCAGCAAGGGGCCTCTTAATACGTCAACACGGTGTTCCGGTCCAAGCTTTGCCTCCACTGACTCATCTCCTCAATATCATGAGTGCCGCAGGTGGGGCCCCAGATTCAGTGGAAAATGGAGAAACCAATGGAGCGAAGGAAGCCAACAACCATCCTTCACATGGCCCAGCAGATGCGAAAACTAACCAACCATCATTGGAGCCGGAAGGTCAACCTCCAGTTGGGTTGGGCAGAGGCTTAGCAGCACTTGATGGCAAGAAACAAAAGTCGAAAGCCAAAGCTGCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1430

Amino Acids

156.27

Weight (kDa)

5.91

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 121 - 197 5.3e-14 Mitochondrial function, CLU-N-term
GSKIP_dom PF05303 283 - 353 8.7e-06 GSKIP domain
CLU PF13236 405 - 686 1.5e-74 Clustered mitochondria
eIF3_p135 PF12807 812 - 996 4.9e-48 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 1073 - 1136 1.2e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 1109 - 1230 1.6e-12 Nephrocystin-3 TPR domain
TPR_12 PF13424 1112 - 1179 1.1e-11 Tetratricopeptide repeat
TPR_12 PF13424 1149 - 1222 6.2e-14 Tetratricopeptide repeat
TPR_10 PF13374 1150 - 1190 1.1e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1734, 3287
AarI CACCTGC 1 cut(s) 4082
AasI GACNNNNNNGTC 1 cut(s) 2848
Acc16I TGCGCA 1 cut(s) 1597
Acc36I ACCTGC 3 cut(s) 2729, 3160, 4082
AccB1I GGYRCC 2 cut(s) 1694, 3620
AccB7I CCANNNNNTGG 1 cut(s) 2956
AccBSI CCGCTC 1 cut(s) 1054
AccI GTMKAC 1 cut(s) 1532
AccII CGCG 2 cut(s) 1589, 3741
AciI CCGC 8 cut(s) 1054, 1279, 1442, 2178, 3251, 3789, 3870, 4089
AclI AACGTT 1 cut(s) 872
AclWI GGATC 8 cut(s) 1376, 1452, 1951, 2071, 2553, 2625, 3087, 3495
AcoI YGGCCR 2 cut(s) 271, 1746
AcsI RAATTY 6 cut(s) 262, 1044, 1105, 1244, 1272, 2864
AcuI CTGAAG 6 cut(s) 108, 510, 1408, 2484, 3149, 3777
AcvI CACGTG 2 cut(s) 2276, 3704
AcyI GRCGYC 1 cut(s) 3621
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 2 cut(s) 580, 3482
AflII CTTAAG 2 cut(s) 2591, 2602
AflIII ACRYGT 4 cut(s) 1398, 1587, 3701, 3813
AhlI ACTAGT 1 cut(s) 176
AjuI GAANNNNNNNTTGG 4 cut(s) 1020, 1052, 3089, 3121
AleI CACNNNNGTG 1 cut(s) 1143
AloI GAACNNNNNNTCC 4 cut(s) 1917, 1949, 2475, 2507
Alw21I GWGCWC 1 cut(s) 1502
AlwI GGATC 8 cut(s) 1376, 1452, 1951, 2071, 2553, 2625, 3087, 3495
AlwNI CAGNNNCTG 1 cut(s) 388
Ama87I CYCGRG 3 cut(s) 1385, 3399, 3942
ApaI GGGCCC 1 cut(s) 4102
ApoI RAATTY 6 cut(s) 262, 1044, 1105, 1244, 1272, 2864
ArsI GACNNNNNNTTYG 6 cut(s) 98, 130, 681, 713, 2132, 2164
AseI ATTAAT 2 cut(s) 2487, 3465
Asp700I GAANNNNTTC 3 cut(s) 531, 3549, 3936
AspLEI GCGC 4 cut(s) 935, 1598, 3623, 3741
AsuC2I CCSGG 3 cut(s) 2754, 3400, 3401
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 3 cut(s) 1385, 3399, 3942
AvaII GGWCC 6 cut(s) 73, 697, 2119, 2520, 3310, 4042
AxyI CCTNAGG 1 cut(s) 1263
BaeGI GKGCMC 1 cut(s) 4102
BaeI ACNNNNGTAYC 2 cut(s) 57, 90
BalI TGGCCA 2 cut(s) 273, 1748
BanI GGYRCC 2 cut(s) 1694, 3620
BanII GRGCYC 3 cut(s) 568, 1291, 4102
BauI CACGAG 2 cut(s) 2043, 3972
BbrPI CACGTG 2 cut(s) 2276, 3704
BbsI GAAGAC 3 cut(s) 2228, 2296, 3731
Bbv12I GWGCWC 1 cut(s) 1502
BbvCI CCTCAGC 1 cut(s) 3111
BcnI CCSGG 3 cut(s) 2754, 3400, 3401
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 4 cut(s) 177, 338, 2715, 2907
BfmI CTRYAG 4 cut(s) 829, 2997, 3039, 3861
BfoI RGCGCY 2 cut(s) 936, 3624
BfrI CTTAAG 2 cut(s) 2591, 2602
BfuAI ACCTGC 3 cut(s) 2729, 3160, 4082
BglII AGATCT 2 cut(s) 554, 2416
BlpI GCTNAGC 1 cut(s) 4240
Bme18I GGWCC 6 cut(s) 73, 697, 2119, 2520, 3310, 4042
BmeT110I CYCGRG 3 cut(s) 1385, 3399, 3942
BmrI ACTGGG 1 cut(s) 3689
BmuI ACTGGG 1 cut(s) 3689
BoxI GACNNNNGTC 1 cut(s) 3394
BpiI GAAGAC 3 cut(s) 2228, 2296, 3731
BplI GAGNNNNNCTC 2 cut(s) 2023, 2055
BpmI CTGGAG 5 cut(s) 209, 696, 2811, 3261, 4203
Bpu10I CCTNAGC 5 cut(s) 279, 702, 2169, 3075, 3111
Bpu1102I GCTNAGC 1 cut(s) 4240
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 2 cut(s) 1469, 2107
BpuMI CCSGG 3 cut(s) 2754, 3400, 3401
BsaAI YACGTR 3 cut(s) 1665, 2276, 3704
BsaBI GATNNNNATC 1 cut(s) 1443
BsaHI GRCGYC 1 cut(s) 3621
BsaI GGTCTC 2 cut(s) 2076, 2083
BsaWI WCCGGW 2 cut(s) 30, 4039
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bse118I RCCGGY 3 cut(s) 737, 1502, 3866
Bse1I ACTGG 9 cut(s) 76, 1127, 1540, 1973, 2065, 2373, 3684, 3722, 4220
Bse21I CCTNAGG 1 cut(s) 1263
Bse3DI GCAATG 1 cut(s) 3228
Bse8I GATNNNNATC 1 cut(s) 1443
BseJI GATNNNNATC 1 cut(s) 1443
BseMI GCAATG 1 cut(s) 3228
BseNI ACTGG 9 cut(s) 76, 1127, 1540, 1973, 2065, 2373, 3684, 3722, 4220
BseRI GAGGAG 6 cut(s) 1238, 1251, 2031, 2351, 3701, 4061
BseSI GKGCMC 1 cut(s) 4102
BseYI CCCAGC 1 cut(s) 4168
BsgI GTGCAG 3 cut(s) 1050, 1953, 3954
Bsh1236I CGCG 2 cut(s) 1589, 3741
BshNI GGYRCC 2 cut(s) 1694, 3620
BsiHKAI GWGCWC 1 cut(s) 1502
BsiHKCI CYCGRG 3 cut(s) 1385, 3399, 3942
BsiSI CCGG 8 cut(s) 31, 738, 1503, 2753, 3400, 3867, 4040, 4205
BslFI GGGAC 6 cut(s) 683, 916, 1706, 2533, 2784, 3382
BsmFI GGGAC 6 cut(s) 683, 916, 1706, 2533, 2784, 3382
BsmI GAATGC 6 cut(s) 1610, 1800, 1985, 3760, 3790, 3845
Bso31I GGTCTC 2 cut(s) 2076, 2083
BsoBI CYCGRG 3 cut(s) 1385, 3399, 3942
Bsp119I TTCGAA 1 cut(s) 1487
Bsp120I GGGCCC 1 cut(s) 4098
Bsp1286I GDGCHC 4 cut(s) 568, 1291, 1502, 4102
Bsp1720I GCTNAGC 1 cut(s) 4240
Bsp19I CCATGG 2 cut(s) 1617, 2442
BspACI CCGC 8 cut(s) 1054, 1279, 1442, 2178, 3251, 3789, 3870, 4089
BspFNI CGCG 2 cut(s) 1589, 3741
BspHI TCATGA 2 cut(s) 2620, 4080
BspMAI CTGCAG 1 cut(s) 3001
BspMI ACCTGC 3 cut(s) 2729, 3160, 4082
BspPI GGATC 8 cut(s) 1376, 1452, 1951, 2071, 2553, 2625, 3087, 3495
BspQI GCTCTTC 1 cut(s) 3516
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 2 cut(s) 1694, 3620
BspTI CTTAAG 2 cut(s) 2591, 2602
BspTNI GGTCTC 2 cut(s) 2076, 2083
BsrBI CCGCTC 1 cut(s) 1054
BsrDI GCAATG 1 cut(s) 3228
BsrFI RCCGGY 3 cut(s) 737, 1502, 3866
BsrI ACTGG 9 cut(s) 76, 1127, 1540, 1973, 2065, 2373, 3684, 3722, 4220
BssAI RCCGGY 3 cut(s) 737, 1502, 3866
BssNI GRCGYC 1 cut(s) 3621
BssSI CACGAG 2 cut(s) 2043, 3972
BssT1I CCWWGG 5 cut(s) 522, 1617, 1698, 1887, 2442
Bst2BI CACGAG 2 cut(s) 2043, 3972
Bst4CI ACNGT 7 cut(s) 70, 163, 384, 1933, 2657, 2763, 4034
Bst6I CTCTTC 3 cut(s) 2801, 3516, 3942
BstACI GRCGYC 1 cut(s) 3621
BstAFI CTTAAG 2 cut(s) 2591, 2602
BstAPI GCANNNNNTGC 2 cut(s) 1166, 2474
BstBAI YACGTR 3 cut(s) 1665, 2276, 3704
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 9 cut(s) 271, 633, 1205, 1659, 2634, 2977, 3743, 3843, 3868
BstDSI CCRYGG 2 cut(s) 1617, 2442
BstEII GGTNACC 2 cut(s) 287, 2173
BstENI CCTNNNNNAGG 1 cut(s) 282
BstFNI CGCG 2 cut(s) 1589, 3741
BstH2I RGCGCY 2 cut(s) 936, 3624
BstHHI GCGC 4 cut(s) 935, 1598, 3623, 3741
BstNSI RCATGY 7 cut(s) 411, 423, 1207, 1402, 2831, 2918, 3817
BstPAI GACNNNNGTC 1 cut(s) 3394
BstPI GGTNACC 2 cut(s) 287, 2173
BstSFI CTRYAG 4 cut(s) 829, 2997, 3039, 3861
BstSLI GKGCMC 1 cut(s) 4102
BstSNI TACGTA 1 cut(s) 1665
BstUI CGCG 2 cut(s) 1589, 3741
BstV2I GAAGAC 3 cut(s) 2228, 2296, 3731
BstX2I RGATCY 8 cut(s) 554, 1444, 1943, 2063, 2416, 2545, 3079, 3487
BstXI CCANNNNNNTGG 4 cut(s) 1110, 1894, 2480, 3491
BstYI RGATCY 8 cut(s) 554, 1444, 1943, 2063, 2416, 2545, 3079, 3487
Bsu36I CCTNAGG 1 cut(s) 1263
BtgI CCRYGG 2 cut(s) 1617, 2442
BtgZI GCGATG 1 cut(s) 612
BtsI GCAGTG 2 cut(s) 3321, 3504
BtsIMutI CAGTG 9 cut(s) 1169, 1336, 1980, 2058, 2307, 3321, 3504, 4058, 4116
BveI ACCTGC 3 cut(s) 2729, 3160, 4082
Cac8I GCNNGC 9 cut(s) 271, 633, 1205, 1659, 2634, 2977, 3743, 3843, 3868
CaiI CAGNNNCTG 1 cut(s) 388
CciI TCATGA 2 cut(s) 2620, 4080
CfoI GCGC 4 cut(s) 935, 1598, 3623, 3741
Cfr10I RCCGGY 3 cut(s) 737, 1502, 3866
Cfr9I CCCGGG 1 cut(s) 3399
CseI GACGC 4 cut(s) 603, 1050, 1578, 1595
Csp6I GTAC 2 cut(s) 579, 3481
CviQI GTAC 2 cut(s) 579, 3481
DinI GGCGCC 1 cut(s) 3622
DraIII CACNNNGTG 1 cut(s) 326
DrdI GACNNNNNNGTC 1 cut(s) 2848
DseDI GACNNNNNNGTC 1 cut(s) 2848
EaeI YGGCCR 2 cut(s) 271, 1746
Eam1104I CTCTTC 3 cut(s) 2801, 3516, 3942
EarI CTCTTC 3 cut(s) 2801, 3516, 3942
Eco105I TACGTA 1 cut(s) 1665
Eco130I CCWWGG 5 cut(s) 522, 1617, 1698, 1887, 2442
Eco147I AGGCCT 1 cut(s) 3152
Eco24I GRGCYC 3 cut(s) 568, 1291, 4102
Eco31I GGTCTC 2 cut(s) 2076, 2083
Eco32I GATATC 1 cut(s) 3022
Eco47I GGWCC 6 cut(s) 73, 697, 2119, 2520, 3310, 4042
Eco57I CTGAAG 6 cut(s) 108, 510, 1408, 2484, 3149, 3777
Eco72I CACGTG 2 cut(s) 2276, 3704
Eco81I CCTNAGG 1 cut(s) 1263
Eco88I CYCGRG 3 cut(s) 1385, 3399, 3942
Eco91I GGTNACC 2 cut(s) 287, 2173
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoO109I RGGNCCY 5 cut(s) 697, 2119, 4012, 4098, 4099
EcoO65I GGTNACC 2 cut(s) 287, 2173
EcoRV GATATC 1 cut(s) 3022
EcoT14I CCWWGG 5 cut(s) 522, 1617, 1698, 1887, 2442
EcoT22I ATGCAT 4 cut(s) 1361, 2829, 2916, 3189
EcoT38I GRGCYC 3 cut(s) 568, 1291, 4102
EgeI GGCGCC 1 cut(s) 3622
EheI GGCGCC 1 cut(s) 3622
ErhI CCWWGG 5 cut(s) 522, 1617, 1698, 1887, 2442
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 2585, 2617
FaqI GGGAC 6 cut(s) 683, 916, 1706, 2533, 2784, 3382
FauI CCCGC 3 cut(s) 1272, 3244, 3863
FauNDI CATATG 2 cut(s) 1470, 3393
FblI GTMKAC 1 cut(s) 1532
FriOI GRGCYC 3 cut(s) 568, 1291, 4102
FspBI CTAG 4 cut(s) 177, 338, 2715, 2907
FspI TGCGCA 1 cut(s) 1597
GlaI GCGC 4 cut(s) 934, 1597, 3622, 3740
GsaI CCCAGC 1 cut(s) 4172
GsuI CTGGAG 5 cut(s) 209, 696, 2811, 3261, 4203
HaeII RGCGCY 2 cut(s) 936, 3624
HapII CCGG 8 cut(s) 31, 738, 1503, 2753, 3400, 3867, 4040, 4205
HgaI GACGC 4 cut(s) 603, 1050, 1578, 1595
HhaI GCGC 4 cut(s) 935, 1598, 3623, 3741
Hin1I GRCGYC 1 cut(s) 3621
Hin6I GCGC 4 cut(s) 933, 1596, 3621, 3739
HinP1I GCGC 4 cut(s) 933, 1596, 3621, 3739
HincII GTYRAC 4 cut(s) 174, 3898, 4028, 4214
HindII GTYRAC 4 cut(s) 174, 3898, 4028, 4214
HindIII AAGCTT 4 cut(s) 887, 1032, 3777, 4047
HpaII CCGG 8 cut(s) 31, 738, 1503, 2753, 3400, 3867, 4040, 4205
Hpy166II GTNNAC 6 cut(s) 174, 1408, 1533, 3898, 4028, 4214
Hpy8I GTNNAC 6 cut(s) 174, 1408, 1533, 3898, 4028, 4214
Hpy99I CGWCG 1 cut(s) 227
HpyCH4III ACNGT 7 cut(s) 70, 163, 384, 1933, 2657, 2763, 4034
HpyCH4IV ACGT 7 cut(s) 872, 1083, 1144, 1664, 2275, 3703, 4024
HpySE526I ACGT 7 cut(s) 872, 1083, 1144, 1664, 2275, 3703, 4024
Hsp92I GRCGYC 1 cut(s) 3621
HspAI GCGC 4 cut(s) 933, 1596, 3621, 3739
KasI GGCGCC 1 cut(s) 3620
KroI GCCGGC 1 cut(s) 3866
KroNI GCCGGC 1 cut(s) 3868
LguI GCTCTTC 1 cut(s) 3516
MaeI CTAG 4 cut(s) 177, 338, 2715, 2907
MaeII ACGT 7 cut(s) 872, 1083, 1144, 1664, 2275, 3703, 4024
MbiI CCGCTC 1 cut(s) 1054
MfeI CAATTG 4 cut(s) 347, 1640, 2967, 3104
MflI RGATCY 8 cut(s) 554, 1444, 1943, 2063, 2416, 2545, 3079, 3487
MhlI GDGCHC 4 cut(s) 568, 1291, 1502, 4102
MlsI TGGCCA 2 cut(s) 273, 1748
MluI ACGCGT 1 cut(s) 1587
MluNI TGGCCA 2 cut(s) 273, 1748
Mly113I GGCGCC 1 cut(s) 3621
MlyI GAGTC 7 cut(s) 122, 137, 179, 1817, 2836, 3701, 4057
MmeI TCCRAC 6 cut(s) 182, 823, 855, 1321, 1400, 3909
Mox20I TGGCCA 2 cut(s) 273, 1748
Mph1103I ATGCAT 4 cut(s) 1361, 2829, 2916, 3189
MroNI GCCGGC 1 cut(s) 3866
MroXI GAANNNNTTC 3 cut(s) 531, 3549, 3936
MscI TGGCCA 2 cut(s) 273, 1748
MslI CAYNNNNRTG 6 cut(s) 1143, 1401, 2478, 3333, 3444, 3642
Msp20I TGGCCA 2 cut(s) 273, 1748
MspA1I CMGCKG 4 cut(s) 269, 1456, 2180, 2258
MspCI CTTAAG 2 cut(s) 2591, 2602
MspI CCGG 8 cut(s) 31, 738, 1503, 2753, 3400, 3867, 4040, 4205
MunI CAATTG 4 cut(s) 347, 1640, 2967, 3104
Mva1269I GAATGC 6 cut(s) 1610, 1800, 1985, 3760, 3790, 3845
MvnI CGCG 2 cut(s) 1589, 3741
NaeI GCCGGC 1 cut(s) 3868
NarI GGCGCC 1 cut(s) 3621
NciI CCSGG 3 cut(s) 2754, 3400, 3401
NcoI CCATGG 2 cut(s) 1617, 2442
NdeI CATATG 2 cut(s) 1470, 3393
NgoMIV GCCGGC 1 cut(s) 3866
NmuCI GTSAC 8 cut(s) 39, 179, 287, 615, 1300, 1583, 2173, 3704
NsbI TGCGCA 1 cut(s) 1597
NsiI ATGCAT 4 cut(s) 1361, 2829, 2916, 3189
NspI RCATGY 7 cut(s) 411, 423, 1207, 1402, 2831, 2918, 3817
NspV TTCGAA 1 cut(s) 1487
OliI CACNNNNGTG 1 cut(s) 1143
PaeI GCATGC 1 cut(s) 1207
PagI TCATGA 2 cut(s) 2620, 4080
PaqCI CACCTGC 1 cut(s) 4082
PceI AGGCCT 1 cut(s) 3152
PciI ACATGT 2 cut(s) 1398, 3813
PciSI GCTCTTC 1 cut(s) 3516
PctI GAATGC 6 cut(s) 1610, 1800, 1985, 3760, 3790, 3845
PdiI GCCGGC 1 cut(s) 3868
PdmI GAANNNNTTC 3 cut(s) 531, 3549, 3936
PfeI GAWTC 9 cut(s) 200, 242, 362, 1622, 2270, 2927, 3028, 3692, 4106
PflMI CCANNNNNTGG 1 cut(s) 2956
PfoI TCCNGGA 2 cut(s) 673, 2752
PleI GAGTC 7 cut(s) 121, 136, 178, 1817, 2836, 3701, 4057
PluTI GGCGCC 1 cut(s) 3624
PmaCI CACGTG 2 cut(s) 2276, 3704
PmlI CACGTG 2 cut(s) 2276, 3704
PpsI GAGTC 7 cut(s) 121, 136, 178, 1817, 2836, 3701, 4057
Ppu21I YACGTR 3 cut(s) 1665, 2276, 3704
PpuMI RGGWCCY 2 cut(s) 697, 2119
PscI ACATGT 2 cut(s) 1398, 3813
PshAI GACNNNNGTC 1 cut(s) 3394
PshBI ATTAAT 2 cut(s) 2487, 3465
PsiI TTATAA 2 cut(s) 1734, 3287
Psp1406I AACGTT 1 cut(s) 872
Psp5II RGGWCCY 2 cut(s) 697, 2119
PspCI CACGTG 2 cut(s) 2276, 3704
PspEI GGTNACC 2 cut(s) 287, 2173
PspFI CCCAGC 1 cut(s) 4168
PspOMI GGGCCC 1 cut(s) 4098
PspPPI RGGWCCY 2 cut(s) 697, 2119
PsrI GAACNNNNNNTAC 2 cut(s) 3369, 3401
PstI CTGCAG 1 cut(s) 3001
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 8 cut(s) 554, 1444, 1943, 2063, 2416, 2545, 3079, 3487
PvuII CAGCTG 3 cut(s) 269, 1456, 2258
RsaI GTAC 2 cut(s) 580, 3482
RsaNI GTAC 2 cut(s) 579, 3481
RseI CAYNNNNRTG 6 cut(s) 1143, 1401, 2478, 3333, 3444, 3642
SapI GCTCTTC 1 cut(s) 3516
SchI GAGTC 7 cut(s) 122, 137, 179, 1817, 2836, 3701, 4057
SduI GDGCHC 4 cut(s) 568, 1291, 1502, 4102
SfcI CTRYAG 4 cut(s) 829, 2997, 3039, 3861
SfoI GGCGCC 1 cut(s) 3622
SfuI TTCGAA 1 cut(s) 1487
SinI GGWCC 6 cut(s) 73, 697, 2119, 2520, 3310, 4042
SmaI CCCGGG 1 cut(s) 3401
SmiMI CAYNNNNRTG 6 cut(s) 1143, 1401, 2478, 3333, 3444, 3642
SmlI CTYRAG 4 cut(s) 1448, 2086, 2591, 2602
SmoI CTYRAG 4 cut(s) 1448, 2086, 2591, 2602
SnaBI TACGTA 1 cut(s) 1665
SpeI ACTAGT 1 cut(s) 176
SphI GCATGC 1 cut(s) 1207
SseBI AGGCCT 1 cut(s) 3152
SsiI CCGC 8 cut(s) 1054, 1279, 1442, 2178, 3251, 3789, 3870, 4089
SspDI GGCGCC 1 cut(s) 3620
SspMI CTAG 4 cut(s) 177, 338, 2715, 2907
StuI AGGCCT 1 cut(s) 3152
StyI CCWWGG 5 cut(s) 522, 1617, 1698, 1887, 2442
TaaI ACNGT 7 cut(s) 70, 163, 384, 1933, 2657, 2763, 4034
TaiI ACGT 7 cut(s) 875, 1086, 1147, 1667, 2278, 3706, 4027
TaqI TCGA 8 cut(s) 116, 225, 1487, 2074, 3514, 3695, 3810, 4271
TauI GCSGC 2 cut(s) 3791, 4091
TfiI GAWTC 9 cut(s) 200, 242, 362, 1622, 2270, 2927, 3028, 3692, 4106
TscAI CASTG 9 cut(s) 1176, 1336, 1980, 2065, 2314, 3328, 3511, 4065, 4116
TseFI GTSAC 8 cut(s) 39, 179, 287, 615, 1300, 1583, 2173, 3704
Tsp45I GTSAC 8 cut(s) 39, 179, 287, 615, 1300, 1583, 2173, 3704
TspGWI ACGGA 3 cut(s) 121, 2627, 2927
TspMI CCCGGG 1 cut(s) 3399
TspRI CASTG 9 cut(s) 1176, 1336, 1980, 2065, 2314, 3328, 3511, 4065, 4116
Van91I CCANNNNNTGG 1 cut(s) 2956
Vha464I CTTAAG 2 cut(s) 2591, 2602
VpaK11BI GGWCC 6 cut(s) 73, 697, 2119, 2520, 3310, 4042
VspI ATTAAT 2 cut(s) 2487, 3465
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 6 cut(s) 262, 1044, 1105, 1244, 1272, 2864
XbaI TCTAGA 1 cut(s) 2714
XceI RCATGY 7 cut(s) 411, 423, 1207, 1402, 2831, 2918, 3817
XcmI CCANNNNNNNNNTGG 3 cut(s) 1107, 1489, 4160
XmaI CCCGGG 1 cut(s) 3399
XmiI GTMKAC 1 cut(s) 1532
XmnI GAANNNNTTC 3 cut(s) 531, 3549, 3936
XspI CTAG 4 cut(s) 177, 338, 2715, 2907
Zsp2I ATGCAT 4 cut(s) 1361, 2829, 2916, 3189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.