Rorug04G0193000

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002268
Physical Location & Seq
Reverse (-)
33618778 .. 33619598
821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0193000.1

Sequence Viewer

Length: 669 bp
ATGACAACCTCAAACGACGATATCTTAATGTCAATGACGACCAGTACTGCTCGCAACTACCCCAAGAAGAAAAGTACTCGAGGAAGCGGAAGGGTGAAAAGGGAATTTCCACCACCAATACTGCTTCTGGCACAAACGGGGAATTTGCAATGTCGCATGCCTTGGAATCTGACTAGGCATTATTCTAATGGAAGGCTAGTTTTGAAAGGAGAGAAAGCACAGCACCATGAGTACTTCGAAGCCGAACGTGACAGCGGCCACCTTGTGTTGAAGCTTGTGCCTCTGGACAGCACCATACTAATGACCTATGATGTGCCTGAATCGTCTGGTGGACATTTGGACGTGTGTGAGGAAAACGATGAAGAGCTTGAAATGGAAAACGTGCAGTTTGCTGAAGAAGACTATGCTGAGGATGAGAATGATGATGCTGATGATGACGAAGATGATGATGATGATGAATGTGACAACAATGTGGATGAGAGTAAAACTATGTTTGTTTGCAATTCGGTCCCTCAGTCACACTCGGAGATCAGCAGGTGTGATGAGAAATATGGAAATCTGCCGATGTATTTCACGAAGTCAGCTGCAAGTGTCCATGAACAACCGCAAAACTACTTTGCACACCCTGCTTCAGCTCCACTTCGTCCAATGACTGCTGTGAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.17

Weight (kDa)

4.54

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 35 - 92 1.2e-12 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 525
AciI CCGC 3 cut(s) 87, 255, 605
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 2 cut(s) 104, 142
AcuI CTGAAG 2 cut(s) 414, 615
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 3 cut(s) 46, 76, 233
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 3 cut(s) 205, 271, 371
AjiI CACGTC 1 cut(s) 343
AluBI AGCT 4 cut(s) 274, 367, 584, 635
AluI AGCT 4 cut(s) 274, 367, 584, 635
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 2 cut(s) 104, 142
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 237
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 405
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 571
BfaI CTAG 2 cut(s) 174, 197
BfuAI ACCTGC 1 cut(s) 525
BisI GCNGC 2 cut(s) 256, 585
BlsI GCNGC 2 cut(s) 257, 586
BmcAI AGTACT 3 cut(s) 46, 76, 233
Bme18I GGWCC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 78
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 1 cut(s) 510
BmsI GCATC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 155
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 418, 481
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 2 cut(s) 399, 527
BseNI ACTGG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 571
BsgI GTGCAG 1 cut(s) 404
BshFI GGCC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 78
BslFI GGGAC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 237
Bsp143I GATC 1 cut(s) 528
BspACI CCGC 3 cut(s) 87, 255, 605
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 2 cut(s) 400, 526
BspLI GGNNCC 1 cut(s) 510
BspMI ACCTGC 1 cut(s) 525
BspQI GCTCTTC 1 cut(s) 357
BspT104I TTCGAA 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 1 cut(s) 42
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 161
Bst6I CTCTTC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 626
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 2 cut(s) 52, 158
BstDEI CTNAG 2 cut(s) 408, 513
BstF5I GGATG 2 cut(s) 418, 481
BstKTI GATC 1 cut(s) 531
BstMBI GATC 1 cut(s) 528
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 1 cut(s) 405
BsuRI GGCC 1 cut(s) 258
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 2 cut(s) 418, 481
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 2 cut(s) 52, 158
Cfr13I GGNCC 1 cut(s) 508
Csp6I GTAC 3 cut(s) 45, 75, 232
CviAII CATG 3 cut(s) 157, 227, 596
CviJI RGCY 7 cut(s) 196, 242, 258, 274, 367, 584, 635
CviKI_1 RGCY 7 cut(s) 196, 242, 258, 274, 367, 584, 635
CviQI GTAC 3 cut(s) 45, 75, 232
DdeI CTNAG 2 cut(s) 408, 513
DpnI GATC 1 cut(s) 530
DpnII GATC 1 cut(s) 528
DraIII CACNNNGTG 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 161
Eco32I GATATC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 2 cut(s) 414, 615
Eco88I CYCGRG 1 cut(s) 78
EcoRV GATATC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 3 cut(s) 160, 230, 599
FaiI YATR 8 cut(s) 158, 228, 296, 309, 405, 491, 552, 597
FaqI GGGAC 1 cut(s) 494
FatI CATG 3 cut(s) 156, 226, 595
Fnu4HI GCNGC 2 cut(s) 256, 585
FokI GGATG 2 cut(s) 425, 488
Fsp4HI GCNGC 2 cut(s) 256, 585
FspBI CTAG 2 cut(s) 174, 197
GluI GCNGC 2 cut(s) 256, 585
HaeIII GGCC 1 cut(s) 258
Hin1II CATG 3 cut(s) 160, 230, 599
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 166, 320
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 332
Hpy188I TCNGA 2 cut(s) 171, 526
Hpy188III TCNNGA 2 cut(s) 284, 574
Hpy8I GTNNAC 1 cut(s) 332
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 84, 186
HpyCH4IV ACGT 3 cut(s) 247, 342, 381
HpyCH4V TGCA 5 cut(s) 148, 385, 501, 587, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpyF3I CTNAG 2 cut(s) 408, 513
HpySE526I ACGT 3 cut(s) 247, 342, 381
Hsp92II CATG 3 cut(s) 160, 230, 599
Kzo9I GATC 1 cut(s) 528
LguI GCTCTTC 1 cut(s) 357
LmnI GCTCC 1 cut(s) 640
LpnPI CCDG 7 cut(s) 55, 113, 269, 312, 330, 520, 639
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 174, 197
MaeII ACGT 3 cut(s) 247, 342, 381
MaeIII GTNAC 3 cut(s) 248, 461, 516
MalI GATC 1 cut(s) 530
MboI GATC 1 cut(s) 528
MboII GAAGA 5 cut(s) 79, 374, 407, 410, 452
MluCI AATT 4 cut(s) 104, 142, 502, 664
MnlI CCTC 6 cut(s) 19, 74, 291, 343, 403, 522
MseI TTAA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 255, 584
MwoI GCNNNNNNNGC 1 cut(s) 626
NdeII GATC 1 cut(s) 528
NlaIII CATG 3 cut(s) 160, 230, 599
NlaIV GGNNCC 1 cut(s) 510
NmuCI GTSAC 3 cut(s) 248, 461, 516
NspI RCATGY 1 cut(s) 160
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 78
PaqCI CACCTGC 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 166, 320
PkrI GCNGC 2 cut(s) 257, 586
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 1 cut(s) 508
PspXI VCTCGAGB 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 3 cut(s) 46, 76, 233
RsaNI GTAC 3 cut(s) 45, 75, 232
RseI CAYNNNNRTG 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 2 cut(s) 256, 585
Sau3AI GATC 1 cut(s) 528
Sau96I GGNCC 1 cut(s) 508
ScaI AGTACT 3 cut(s) 46, 76, 233
SfaNI GCATC 1 cut(s) 415
Sfr274I CTCGAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 1 cut(s) 508
SlaI CTCGAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SphI GCATGC 1 cut(s) 160
Sse9I AATT 4 cut(s) 104, 142, 502, 664
SsiI CCGC 3 cut(s) 87, 255, 605
SspMI CTAG 2 cut(s) 174, 197
StyI CCWWGG 1 cut(s) 161
TaiI ACGT 3 cut(s) 250, 345, 384
TaqI TCGA 2 cut(s) 79, 237
TaqII GACCGA 1 cut(s) 496
TasI AATT 4 cut(s) 104, 142, 502, 664
TatI WGTACW 3 cut(s) 44, 74, 231
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 2 cut(s) 166, 320
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TseFI GTSAC 3 cut(s) 248, 461, 516
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 3 cut(s) 248, 461, 516
TspDTI ATGAA 3 cut(s) 375, 471, 612
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 2 cut(s) 104, 142
XceI RCATGY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 78
XspI CTAG 2 cut(s) 174, 197
ZrmI AGTACT 3 cut(s) 46, 76, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.