Rh5CG159200

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002268
Physical Location & Seq
Reverse (-)
14629587 .. 14643545
13959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG159200.1

Sequence Viewer

Length: 4791 bp
ATGGGCTTGAGAGGCGGGGGAGACACCGATCTACACCCTACCCTTCGTGGTAGTTCCCACTTCCCTCACCCCTTCCATCATATCCAATCTTCTTTCTTTTTCACCATTATTCCAACCTTCCCTCTGTTTCTCCCCAATTTCCAATCCCTCTATTCTTCCTTCAGAAGCTTGTACCTCTTCCCAGTCATCAACGCCATCTTAAGCGATTTCTCGCTATCTCAACAGAGTCAGGCCTTCGCTTGCGATGCTGGCTCTTTCGCTCCTTACTTTCTTTTTTCTTTTTCTTTGGTTCCCTTGTTGTCAGGCAACTTGGCTGTGCTAGTGGCGCTCTTCTTTGGCTATAGAGTTTGGTTCGCGGGGATCTCCTGTGTCTGTGGATGGGATCTGTTCTGGTGTTCTTTCCAGGCATATATGCAATTTTCAAAATCAAAAGAGATGGACGAGGAGGAGTTAACAAGAAGGATGAAGGAGAAAGAGGATGCTATACGCCTGTATTTGACACCAAGAAATGGTCTTCCTGAAGATATCACGAAACAAATCATGGCAAACATAAAACATGACATATTGAAAAAGAAACTTGATACAGTTGTAGATATGGACCTTTTGCTCTCACTTGTTCCCTGGAATATTGAAATCTCCATCAAGCAGCACGTGGGCATGAATGCGGTCAAAAATGTACGAGTGCTTCAAGGTATGGATGTAAGGGTGTTGGAAAAGATCTGCGAGTATCTGAAGCCAGTGACCTACCCAGATGGCCACAAATTCCAAACAAAAGATGCAATTGATTCGATGCTTTTCATCACAGAAGGCGAAATTATATCTACGATTTTACCGTGTCAAGAGAAGCTTGATTGTTTCGGTGAACAACTTTTGTGGTGGGTATCACCGAGTATCTCCTGTACTAACCTTGATCATCCTAAGCCGACCCAAAATGTCCAGTGCCATGGAAAAGTAGAAGCATTTGCTCTCCTGGCCAAGGATTTGAAAAGTGTAGTCACTGAATTCAGACGTCAATGGAATTGGGGTTTATACAGATGTGACACTGGTTCTCCTGATTTGCCACGTCCCCGCGACATGACTAATGAGACATATATTCGGTTGTGGCAGCAAAAATTAATCGAGGCACGGAAGAATAAAATCAGCGAAGAGATACTTAAATGGCTGGAAAGTACTTGTCTCGGTGACGAGGTTGTGAATCGAAAAGTAAGGAAACACATAAGAGAAACTAACGTAGAGAAAAAAGAGATGGATGCTGTTGTGGATCTGCGTTTCATATTCTCTATTCTTCCCCAGGATATCAAAAGTACTATCGGCATGAAGGCGCTGAGGAATGTAGACTTGTTTAAAAAAATGCCAGATTCCATGCTGGAAAATGTCTCAATGTATCTCCATCCAGTGATCTACGACGAGAATAGCTACGTTGTTAAAGCAGGAACTAAGCTTGATTTGATGCTCTTCGTTATAAAAGGATTAATTATATGCACCAACACTTCTACGGATGTTGCAACGACAGGGTCCTATATGATCCCCAAGCTATTTCTTGAGAAAGGTACTATTTATGGAGAAACACTTTTGAGTTGGGCATCATCCTCGAGTAGCAGCGTTCCCGACCTTCCCATCTCTACTCAAGAGTTCAAGTGTCAAAAAAGAGTTGAAGCTTTTTCTCTCAAGGCCTCGCAACTGCAAGATGTAGTCTCCAGATTCGGAAACAAGTGGAGTGAATATTTCACTGCCCCTAATCATGAAAACAAGACTTATCTACAGAAGCTAAAAGAGGTCCATAAGCAGTTGGCTACTACAACATGGGCGGAGATCGACGATGAGGAGTTTAAACAGCAGATGAAGATCATGACCAGGGAGAAAGATATACTAGACTGGCTTTTAAGAATTGATTTCCCTGAAGATGTGACAACAAAAGTCATGGAAAATATCAAAGAGAAGAAAGTAGTGGAGAAAGACGTAGATCGTCCCGCAGATTGCCCCGTAGATGTGGAGTATCTACTCTCTGTTGATCTCCCTTGGACTATCGAAAAGCGTTTAAGGGACCATCTCTGCATGCATACGCTGGAGAAAGTACCTCTGCTTCAAGATATGCCAAAAGCCATTTTGGAGTCTATCTGCAGTAATCTCAAGCCGGTGGTCTACAGAAACAACAATCACTACGTTGTTAAAAAAGGAGAAGAACTTGATCTGATGCTCATTATTGTAGAAGGAGAAATAATATTGACTGATAATGACCCGGTTAGTAGCGAAGCTGAAACGACGAATTCTTCAATCATCCCACAAAGTCTTGGGAAAGGCCATTTTTTTGGGGATGAACTTTTGAATTGGTTCTCACCCGCAAATAAATTTGCCGGCTCTGCTCCAATCTCCCCCCACAATGTCCTCTGTCAAACGAAAGTAGAAGCCTTCACTCTGACGAGAAGTATGTTGAGAAGTGTAGTGTCCGACCACAACAGTGATTGGATGTCAAAATTCAAAAATGTTGACGATGCGCAGCAGTTGAAGGATTTGAAAATTCTTGACGAAAGGATTGATGAGCAGTTAGGAAAAACAAATTTGGACGAGATTGTGGATGAAGAGTCAGAACAGCAGGTGAAAATCAAGAACAAGGAGAAAGAAATAGATAACTGGGTATCAAGAAATGGTTTTGATGAGGAATTGAAGACAAAAATCAGGTTTCACATGAAAATAAATAATGCAGTTGAAGAAAATATAGATGCTGAAGTAGATGTGGAGTATTTATTCTCTCTTAAATTTCCATGGATTGATATTGCCAAACCTGTAACGGAGCATCTCTGTATGGGCACCCTTAAAAAGGTACCCATGCTTCAGAAAATGCCTGAAGACGTCTGGAAAAAGATGTGCTTCCATTTGGAGGCAGTGGTCTACAACGAGAAAGAGTTTGTGGTTCGAGAAGGACAATCATTCGATTTTGTGCTCATCATTGTGGAAGGTGAAATTGAATGGACAATAGCGACTAGTGATGGTGGACCAGCGGATTCCACATTAATCACCAAGGGGAGAGGTGATTTTTTCGGAGAAAGACTTCTGAACTGGGCATCACCCAAAATCATGTTTTTTGGCTCTGCTCCTATCTCAAAAAGTGATGTCAAATGTAAAACAAAAGTAGAAGCCTTTCGTCTAACAACCAAACACTTGAGAAGTGTAGTCTCTGAATACGACCTTGAGTGGAATTCAAAGTTCATCGATTGCAATGACCCGCAACAGATGTATATGCAGTGGCAAAAGGGAACCATAAAACTGAATGAAATTGAGGATGAAGAGTTAAGAAAGGCAGTGAAGATCAAGATCAAGGAGAACGACATATTTGAGTGGATGTCAAGCAACGGCCTCAGTGAAGAATTGAAGGAAAAAATCAAGTTAGAGATACAAAAGAGAAATGCAGTCGAAAGGAATATAAATACTGACTTGTATTTGAAATTTATATTATCACTTGGAGGTATATGGGATGACATAACTAAAGGTATAACAGAGCATTTCTGTATCGACACGCTAAAGAGAGTACCCATGCTTCAACTTTTGCATGAAGATGTCTTTAAAAAGATGTCCTTCCATGTCGAGGCAGTTTTCTTCAATGAAAATGACTTCATCGTTCAAAAAGGGCAATCATTGGATTTTGTGCTCATTATTGTGGAAGGCTTAATTGAATGGACCAACATGACTAGTGATGTTGAAACAACAGATCCCACGATAATCACCAAGGAGAAAGGTGATATTTTTGGAGAAAAACTTCTAAATTGGGCATCTCCCAGCATCATGTTTTCTGGCTCTGCTCCAACCTCCACTGAAGATGTCTTATGTCGAAAGAAAATACAAGCCTTTAGCCTGACCGCTGAAAACTTGAGAAGTGTGGTCTATGATTACAAGCGCGAGTGGAATTTGAATTTCAAAAATTGCAATGGTCAGCAACAATCGGAGGTGGCTACAATTGTCAACAACAAAATTGATGAGACATATAAGCAGTTGCAGTTGGGAACCATAATACTGAATGATATTGAGGATGAAGAGTTAAGAAAGGCAATGAAGATCAAGATCAAGGAGAATGACATATTTGAGTGGATGTCAAGCAACGGCCTCAGTGAAGAATTGACAGAAAAAATCAAGTTAGAGATACAAAAGAGAAATGCAATCGAAAGGAATATAGATACTGACTTGTATTTGAAATTTATATTATCACTCGGAGGTATATGGGATGACATAACTAAAGGTATAACAGAGCATTTCTGTATCAGCACGCTAAAGAGAGTACCCATGCTTCAACTTTTGCATGAAGATGTCTTTAAGAAGATGTCATTCCATGTCGAGGCAGTCGTCTTCAACGAAAATGACTTCATCGTTCAAAAAGGGCAATCATTGAATTTTGTGCTCATTATTGTGGAAGGTGTAATTGAATGGACCGACATGACTAGTGATGTTGAAACAGCAGATCCCACAATAATCACCAAAAAGAAAGGTGATATTTTTGGAGAAAAACTTCTGAATTGGGCATCACCTGAGATCATGTTTTTAGGCTCTGCTCCTACCTCGATGAGAGATGTCAAGTGTAGAAAGAAAGTACAAGCCTTTAGCCTGACTGCTAAAAATCTAAGAAGTGTGGTCTATGAATACAAACACGAGTGGAATTCAAATTTCAAAAGTTGCAACGATCAGCAGCAGTTGGAAGGTGTTATAGTTCTCCGCAACAACATTGATAAGGTATTGAACGAGGCTTTGGATAAAATTCATAAAAGAGTCTCAAATGCATTTTTTTTTTCTCCCATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1596

Amino Acids

183.92

Weight (kDa)

5.53

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 963 - 1060 7.8e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1463
AarI CACCTGC 1 cut(s) 2584
AatII GACGTC 2 cut(s) 1012, 2853
Acc16I TGCGCA 1 cut(s) 2496
Acc36I ACCTGC 1 cut(s) 2584
Acc65I GGTACC 1 cut(s) 2821
AccB1I GGYRCC 2 cut(s) 2807, 2821
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 3 cut(s) 1335, 2142, 2889
AccII CGCG 3 cut(s) 356, 1071, 3894
AclWI GGATC 6 cut(s) 368, 390, 1269, 1519, 3701, 4445
AcoI YGGCCR 2 cut(s) 754, 972
AcuI CTGAAG 8 cut(s) 145, 540, 752, 1920, 2745, 2816, 2865, 3831
AcvI CACGTG 1 cut(s) 652
AcyI GRCGYC 2 cut(s) 1009, 2850
AfiI CCNNNNNNNGG 6 cut(s) 509, 976, 2818, 2878, 3583, 4327
AflII CTTAAG 1 cut(s) 199
AhlI ACTAGT 3 cut(s) 2981, 3686, 4430
AjiI CACGTC 1 cut(s) 1064
AjnI CCWGG 5 cut(s) 402, 620, 969, 1290, 1853
AjuI GAANNNNNNNTTGG 2 cut(s) 4718, 4750
AleI CACNNNNGTG 1 cut(s) 2457
AluBI AGCT 8 cut(s) 168, 847, 1416, 1441, 1534, 1658, 1768, 2255
AluI AGCT 8 cut(s) 168, 847, 1416, 1441, 1534, 1658, 1768, 2255
Alw21I GWGCWC 3 cut(s) 2943, 3648, 4392
Alw26I GTCTC 8 cut(s) 15, 1079, 1181, 1381, 1699, 3178, 3968, 4762
AlwI GGATC 6 cut(s) 368, 390, 1269, 1519, 3701, 4445
Ama87I CYCGRG 1 cut(s) 1591
AoxI GGCC 7 cut(s) 231, 754, 972, 1671, 2299, 3352, 4096
ApeKI GCWGC 5 cut(s) 646, 1105, 1599, 2497, 4675
AseI ATTAAT 3 cut(s) 1115, 1472, 3011
Asp700I GAANNNNTTC 5 cut(s) 2330, 3048, 3138, 3753, 4497
Asp718I GGTACC 1 cut(s) 2821
AspLEI GCGC 4 cut(s) 328, 1324, 2497, 3894
AspS9I GGNCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
AsuC2I CCSGG 1 cut(s) 2240
AvaI CYCGRG 1 cut(s) 1591
AvaII GGWCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
BaeGI GKGCMC 1 cut(s) 2810
BaeI ACNNNNGTAYC 2 cut(s) 573, 606
BalI TGGCCA 2 cut(s) 756, 974
BanI GGYRCC 2 cut(s) 2807, 2821
BauI CACGAG 1 cut(s) 4637
BbrPI CACGTG 1 cut(s) 652
BbsI GAAGAC 4 cut(s) 506, 2672, 2853, 4330
Bbv12I GWGCWC 3 cut(s) 2943, 3648, 4392
BbvCI CCTCAGC 1 cut(s) 1325
BbvI GCAGC 5 cut(s) 658, 1117, 1611, 2509, 4687
BceAI ACGGC 2 cut(s) 3367, 4111
BcgI CGANNNNNNTGC 4 cut(s) 768, 802, 1572, 1606
BciT130I CCWGG 5 cut(s) 404, 622, 971, 1292, 1855
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 2240
BcoDI GTCTC 8 cut(s) 15, 1079, 1181, 1381, 1699, 3178, 3968, 4762
BcuI ACTAGT 3 cut(s) 2981, 3686, 4430
BfaI CTAG 5 cut(s) 320, 1871, 2982, 3687, 4431
BfmI CTRYAG 4 cut(s) 340, 1760, 2119, 2143
BfoI RGCGCY 2 cut(s) 329, 1325
BfrI CTTAAG 1 cut(s) 199
BfuAI ACCTGC 1 cut(s) 2584
BglII AGATCT 1 cut(s) 717
BisI GCNGC 5 cut(s) 647, 1106, 1600, 2498, 4676
BlsI GCNGC 5 cut(s) 648, 1107, 1601, 2499, 4677
BmcAI AGTACT 2 cut(s) 1171, 1306
Bme1390I CCNGG 6 cut(s) 404, 622, 971, 1292, 1855, 2240
Bme18I GGWCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
BmeT110I CYCGRG 1 cut(s) 1591
BmgBI CACGTC 1 cut(s) 1064
BmgT120I GGNCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
BmiI GGNNCC 7 cut(s) 291, 1516, 2045, 2809, 2823, 3256, 4000
BmrFI CCNGG 6 cut(s) 404, 622, 971, 1292, 1855, 2240
BmrI ACTGGG 3 cut(s) 176, 2641, 3067
BmuI ACTGGG 3 cut(s) 176, 2641, 3067
BpiI GAAGAC 4 cut(s) 506, 2672, 2853, 4330
BpmI CTGGAG 2 cut(s) 1681, 2087
Bpu10I CCTNAGC 2 cut(s) 918, 1325
BpuEI CTTGAG 8 cut(s) 28, 1562, 1611, 1652, 2114, 3181, 3209, 3886
BpuMI CCSGG 1 cut(s) 2240
Bsa29I ATCGAT 1 cut(s) 3210
BsaAI YACGTR 1 cut(s) 652
BsaBI GATNNNNATC 3 cut(s) 1844, 3311, 4055
BsaHI GRCGYC 2 cut(s) 1009, 2850
BsaJI CCNNGG 9 cut(s) 620, 943, 975, 1290, 1854, 2018, 2762, 3018, 3723
BsaXI ACNNNNNCTCC 2 cut(s) 1033, 1063
Bsc4I CCNNNNNNNGG 6 cut(s) 509, 976, 2818, 2878, 3583, 4327
Bse118I RCCGGY 2 cut(s) 2134, 2354
Bse1I ACTGG 8 cut(s) 182, 737, 937, 1048, 1394, 1880, 2636, 3062
Bse3DI GCAATG 3 cut(s) 3223, 3928, 4050
Bse8I GATNNNNATC 3 cut(s) 1844, 3311, 4055
BseBI CCWGG 5 cut(s) 404, 622, 971, 1292, 1855
BseCI ATCGAT 1 cut(s) 3210
BseDI CCNNGG 9 cut(s) 620, 943, 975, 1290, 1854, 2018, 2762, 3018, 3723
BseJI GATNNNNATC 3 cut(s) 1844, 3311, 4055
BseLI CCNNNNNNNGG 6 cut(s) 509, 976, 2818, 2878, 3583, 4327
BseMI GCAATG 3 cut(s) 3223, 3928, 4050
BseMII CTCAG 4 cut(s) 1316, 3370, 4114, 4509
BseNI ACTGG 8 cut(s) 182, 737, 937, 1048, 1394, 1880, 2636, 3062
BseRI GAGGAG 3 cut(s) 458, 461, 1838
BseSI GKGCMC 1 cut(s) 2810
BseXI GCAGC 5 cut(s) 658, 1117, 1611, 2509, 4687
BseYI CCCAGC 1 cut(s) 3773
Bsh1236I CGCG 3 cut(s) 356, 1071, 3894
BshFI GGCC 7 cut(s) 233, 756, 974, 1673, 2301, 3354, 4098
BshNI GGYRCC 2 cut(s) 2807, 2821
BshVI ATCGAT 1 cut(s) 3210
BsiHKAI GWGCWC 3 cut(s) 2943, 3648, 4392
BsiHKCI CYCGRG 1 cut(s) 1591
BsiSI CCGG 3 cut(s) 2135, 2240, 2355
BslFI GGGAC 3 cut(s) 1050, 1953, 2057
BslI CCNNNNNNNGG 6 cut(s) 509, 976, 2818, 2878, 3583, 4327
BsmAI GTCTC 8 cut(s) 15, 1079, 1181, 1381, 1699, 3178, 3968, 4762
BsmFI GGGAC 3 cut(s) 1050, 1953, 2057
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 7 cut(s) 233, 756, 974, 1673, 2301, 3354, 4098
BsoBI CYCGRG 1 cut(s) 1591
Bsp1286I GDGCHC 4 cut(s) 2810, 2943, 3648, 4392
Bsp19I CCATGG 2 cut(s) 943, 2762
BspANI GGCC 7 cut(s) 233, 756, 974, 1673, 2301, 3354, 4098
BspCNI CTCAG 4 cut(s) 1317, 3369, 4113, 4510
BspDI ATCGAT 1 cut(s) 3210
BspFNI CGCG 3 cut(s) 356, 1071, 3894
BspHI TCATGA 2 cut(s) 1741, 1848
BspLI GGNNCC 7 cut(s) 291, 1516, 2045, 2809, 2823, 3256, 4000
BspMAI CTGCAG 1 cut(s) 2123
BspMI ACCTGC 1 cut(s) 2584
BspPI GGATC 6 cut(s) 368, 390, 1269, 1519, 3701, 4445
BspQI GCTCTTC 2 cut(s) 335, 1460
BspT107I GGYRCC 2 cut(s) 2807, 2821
BspTI CTTAAG 1 cut(s) 199
BsrDI GCAATG 3 cut(s) 3223, 3928, 4050
BsrFI RCCGGY 2 cut(s) 2134, 2354
BsrI ACTGG 8 cut(s) 182, 737, 937, 1048, 1394, 1880, 2636, 3062
BssAI RCCGGY 2 cut(s) 2134, 2354
BssECI CCNNGG 9 cut(s) 620, 943, 975, 1290, 1854, 2018, 2762, 3018, 3723
BssNI GRCGYC 2 cut(s) 1009, 2850
BssSI CACGAG 1 cut(s) 4637
BssT1I CCWWGG 6 cut(s) 943, 975, 2018, 2762, 3018, 3723
Bst2BI CACGAG 1 cut(s) 4637
Bst2UI CCWGG 5 cut(s) 404, 622, 971, 1292, 1855
Bst4CI ACNGT 3 cut(s) 586, 834, 2459
Bst6I CTCTTC 7 cut(s) 182, 335, 1140, 1460, 2574, 3279, 4023
BstACI GRCGYC 2 cut(s) 1009, 2850
BstAFI CTTAAG 1 cut(s) 199
BstBAI YACGTR 1 cut(s) 652
BstC8I GCNNGC 5 cut(s) 241, 250, 2057, 2356, 4259
BstDEI CTNAG 7 cut(s) 918, 1325, 1437, 3356, 4100, 4518, 4610
BstDSI CCRYGG 2 cut(s) 943, 2762
BstENI CCTNNNNNAGG 2 cut(s) 974, 2816
BstFNI CGCG 3 cut(s) 356, 1071, 3894
BstH2I RGCGCY 2 cut(s) 329, 1325
BstHHI GCGC 4 cut(s) 328, 1324, 2497, 3894
BstMAI GTCTC 8 cut(s) 15, 1079, 1181, 1381, 1699, 3178, 3968, 4762
BstMWI GCNNNNNNNGC 6 cut(s) 12, 245, 249, 325, 971, 2360
BstNI CCWGG 5 cut(s) 404, 622, 971, 1292, 1855
BstNSI RCATGY 1 cut(s) 2059
BstSCI CCNGG 6 cut(s) 402, 620, 969, 1290, 1853, 2238
BstSFI CTRYAG 4 cut(s) 340, 1760, 2119, 2143
BstSLI GKGCMC 1 cut(s) 2810
BstUI CGCG 3 cut(s) 356, 1071, 3894
BstV1I GCAGC 5 cut(s) 658, 1117, 1611, 2509, 4687
BstV2I GAAGAC 4 cut(s) 506, 2672, 2853, 4330
BstX2I RGATCY 6 cut(s) 360, 382, 717, 1261, 3706, 4450
BstXI CCANNNNNNTGG 2 cut(s) 944, 2309
BstYI RGATCY 6 cut(s) 360, 382, 717, 1261, 3706, 4450
Bsu15I ATCGAT 1 cut(s) 3210
BsuRI GGCC 7 cut(s) 233, 756, 974, 1673, 2301, 3354, 4098
BsuTUI ATCGAT 1 cut(s) 3210
BtgI CCRYGG 2 cut(s) 943, 2762
BtgZI GCGATG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 1064
BtsI GCAGTG 4 cut(s) 1728, 2889, 3248, 3306
BveI ACCTGC 1 cut(s) 2584
Cac8I GCNNGC 5 cut(s) 241, 250, 2057, 2356, 4259
CciI TCATGA 2 cut(s) 1741, 1848
CfoI GCGC 4 cut(s) 328, 1324, 2497, 3894
Cfr10I RCCGGY 2 cut(s) 2134, 2354
Cfr13I GGNCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
ClaI ATCGAT 1 cut(s) 3210
CspCI CAANNNNNGTGG 2 cut(s) 854, 889
DdeI CTNAG 7 cut(s) 918, 1325, 1437, 3356, 4100, 4518, 4610
DraI TTTAAA 3 cut(s) 1345, 1831, 3562
EaeI YGGCCR 2 cut(s) 754, 972
Eam1104I CTCTTC 7 cut(s) 182, 335, 1140, 1460, 2574, 3279, 4023
EarI CTCTTC 7 cut(s) 182, 335, 1140, 1460, 2574, 3279, 4023
EciI GGCGGA 1 cut(s) 1823
Eco130I CCWWGG 6 cut(s) 943, 975, 2018, 2762, 3018, 3723
Eco147I AGGCCT 2 cut(s) 233, 1673
Eco32I GATATC 2 cut(s) 526, 1297
Eco47I GGWCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
Eco57I CTGAAG 8 cut(s) 145, 540, 752, 1920, 2745, 2816, 2865, 3831
Eco72I CACGTG 1 cut(s) 652
Eco88I CYCGRG 1 cut(s) 1591
EcoNI CCTNNNNNAGG 2 cut(s) 974, 2816
EcoO109I RGGNCCY 1 cut(s) 1515
EcoRI GAATTC 4 cut(s) 1001, 2266, 3196, 4645
EcoRII CCWGG 5 cut(s) 402, 620, 969, 1290, 1853
EcoRV GATATC 2 cut(s) 526, 1297
EcoT14I CCWWGG 6 cut(s) 943, 975, 2018, 2762, 3018, 3723
EcoT22I ATGCAT 2 cut(s) 2061, 4768
ErhI CCWWGG 6 cut(s) 943, 975, 2018, 2762, 3018, 3723
FalI AAGNNNNNCTT 8 cut(s) 831, 863, 1137, 1169, 2852, 2884, 3557, 3589
FaqI GGGAC 3 cut(s) 1050, 1953, 2057
FauI CCCGC 6 cut(s) 8, 349, 1076, 1978, 2347, 3231
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 3 cut(s) 1335, 2142, 2889
Fnu4HI GCNGC 5 cut(s) 647, 1106, 1600, 2498, 4676
Fsp4HI GCNGC 5 cut(s) 647, 1106, 1600, 2498, 4676
FspBI CTAG 5 cut(s) 320, 1871, 2982, 3687, 4431
FspI TGCGCA 1 cut(s) 2496
GlaI GCGC 4 cut(s) 327, 1323, 2496, 3893
GluI GCNGC 5 cut(s) 647, 1106, 1600, 2498, 4676
GsaI CCCAGC 1 cut(s) 3777
GsuI CTGGAG 2 cut(s) 1681, 2087
HaeII RGCGCY 2 cut(s) 329, 1325
HaeIII GGCC 7 cut(s) 233, 756, 974, 1673, 2301, 3354, 4098
HapII CCGG 3 cut(s) 2135, 2240, 2355
HhaI GCGC 4 cut(s) 328, 1324, 2497, 3894
Hin1I GRCGYC 2 cut(s) 1009, 2850
Hin6I GCGC 4 cut(s) 326, 1322, 2495, 3892
HinP1I GCGC 4 cut(s) 326, 1322, 2495, 3892
HincII GTYRAC 3 cut(s) 453, 2488, 3958
HindII GTYRAC 3 cut(s) 453, 2488, 3958
HindIII AAGCTT 4 cut(s) 166, 845, 1439, 1656
HinfI GANTC 9 cut(s) 226, 785, 1195, 1358, 1701, 2111, 2582, 3002, 4755
HpaI GTTAAC 1 cut(s) 453
HpaII CCGG 3 cut(s) 2135, 2240, 2355
Hpy166II GTNNAC 8 cut(s) 453, 863, 1336, 2143, 2488, 2890, 2993, 3958
Hpy8I GTNNAC 8 cut(s) 453, 863, 1336, 2143, 2488, 2890, 2993, 3958
Hpy99I CGWCG 3 cut(s) 1409, 1820, 2266
HpyCH4III ACNGT 3 cut(s) 586, 834, 2459
HpyCH4IV ACGT 8 cut(s) 651, 1009, 1063, 1230, 1419, 1959, 2163, 2850
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 245, 249, 325, 971, 2360
HpyF3I CTNAG 7 cut(s) 918, 1325, 1437, 3356, 4100, 4518, 4610
HpySE526I ACGT 8 cut(s) 651, 1009, 1063, 1230, 1419, 1959, 2163, 2850
Hsp92I GRCGYC 2 cut(s) 1009, 2850
HspAI GCGC 4 cut(s) 326, 1322, 2495, 3892
KpnI GGTACC 1 cut(s) 2825
KroI GCCGGC 1 cut(s) 2354
KroNI GCCGGC 1 cut(s) 2356
Ksp22I TGATCA 1 cut(s) 910
KspAI GTTAAC 1 cut(s) 453
LguI GCTCTTC 2 cut(s) 335, 1460
LmnI GCTCC 6 cut(s) 265, 2368, 2791, 3097, 3802, 4546
Lsp1109I GCAGC 5 cut(s) 658, 1117, 1611, 2509, 4687
MaeI CTAG 5 cut(s) 320, 1871, 2982, 3687, 4431
MaeII ACGT 8 cut(s) 651, 1009, 1063, 1230, 1419, 1959, 2163, 2850
MaeIII GTNAC 6 cut(s) 739, 994, 1037, 1181, 1906, 2785
MfeI CAATTG 2 cut(s) 780, 3951
MflI RGATCY 6 cut(s) 360, 382, 717, 1261, 3706, 4450
MhlI GDGCHC 4 cut(s) 2810, 2943, 3648, 4392
MlsI TGGCCA 2 cut(s) 756, 974
MluNI TGGCCA 2 cut(s) 756, 974
MlyI GAGTC 4 cut(s) 235, 2120, 2591, 4764
MmeI TCCRAC 5 cut(s) 137, 690, 2472, 3824, 4662
Mox20I TGGCCA 2 cut(s) 756, 974
Mph1103I ATGCAT 2 cut(s) 2061, 4768
MroNI GCCGGC 1 cut(s) 2354
MroXI GAANNNNTTC 5 cut(s) 2330, 3048, 3138, 3753, 4497
MscI TGGCCA 2 cut(s) 756, 974
MslI CAYNNNNRTG 6 cut(s) 2457, 2948, 3552, 3653, 4296, 4397
Msp20I TGGCCA 2 cut(s) 756, 974
MspA1I CMGCKG 2 cut(s) 2999, 3857
MspCI CTTAAG 1 cut(s) 199
MspI CCGG 3 cut(s) 2135, 2240, 2355
MspR9I CCNGG 6 cut(s) 404, 622, 971, 1292, 1855, 2240
MssI GTTTAAAC 1 cut(s) 1831
MunI CAATTG 2 cut(s) 780, 3951
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 5 cut(s) 404, 622, 971, 1292, 1855
MvnI CGCG 3 cut(s) 356, 1071, 3894
MwoI GCNNNNNNNGC 6 cut(s) 12, 245, 249, 325, 971, 2360
NaeI GCCGGC 1 cut(s) 2356
NciI CCSGG 1 cut(s) 2240
NcoI CCATGG 2 cut(s) 943, 2762
NgoMIV GCCGGC 1 cut(s) 2354
NlaIV GGNNCC 7 cut(s) 291, 1516, 2045, 2809, 2823, 3256, 4000
NmuCI GTSAC 5 cut(s) 739, 994, 1037, 1181, 1906
NsbI TGCGCA 1 cut(s) 2496
NsiI ATGCAT 2 cut(s) 2061, 4768
NspI RCATGY 1 cut(s) 2059
OliI CACNNNNGTG 1 cut(s) 2457
PaeI GCATGC 1 cut(s) 2059
PaeR7I CTCGAG 1 cut(s) 1591
PagI TCATGA 2 cut(s) 1741, 1848
PaqCI CACCTGC 1 cut(s) 2584
PceI AGGCCT 2 cut(s) 233, 1673
PciSI GCTCTTC 2 cut(s) 335, 1460
PcsI WCGNNNNNNNCGW 3 cut(s) 830, 4332, 4341
PctI GAATGC 1 cut(s) 667
PdiI GCCGGC 1 cut(s) 2356
PdmI GAANNNNTTC 5 cut(s) 2330, 3048, 3138, 3753, 4497
PfeI GAWTC 5 cut(s) 785, 1195, 1358, 1701, 3002
PflFI GACNNNGTC 1 cut(s) 1513
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 5 cut(s) 648, 1107, 1601, 2499, 4677
PleI GAGTC 4 cut(s) 234, 2119, 2590, 4763
PmaCI CACGTG 1 cut(s) 652
PmeI GTTTAAAC 1 cut(s) 1831
PmlI CACGTG 1 cut(s) 652
PpsI GAGTC 4 cut(s) 234, 2119, 2590, 4763
Ppu21I YACGTR 1 cut(s) 652
PpuMI RGGWCCY 1 cut(s) 1515
PshBI ATTAAT 3 cut(s) 1115, 1472, 3011
PsiI TTATAA 1 cut(s) 1463
Psp5II RGGWCCY 1 cut(s) 1515
Psp6I CCWGG 5 cut(s) 402, 620, 969, 1290, 1853
PspCI CACGTG 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 3773
PspGI CCWGG 5 cut(s) 402, 620, 969, 1290, 1853
PspN4I GGNNCC 7 cut(s) 291, 1516, 2045, 2809, 2823, 3256, 4000
PspPI GGNCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
PspPPI RGGWCCY 1 cut(s) 1515
PspXI VCTCGAGB 1 cut(s) 1591
PstI CTGCAG 1 cut(s) 2123
PsuI RGATCY 6 cut(s) 360, 382, 717, 1261, 3706, 4450
PsyI GACNNNGTC 1 cut(s) 1513
RseI CAYNNNNRTG 6 cut(s) 2457, 2948, 3552, 3653, 4296, 4397
SapI GCTCTTC 2 cut(s) 335, 1460
SatI GCNGC 5 cut(s) 647, 1106, 1600, 2498, 4676
Sau96I GGNCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
ScaI AGTACT 2 cut(s) 1171, 1306
SchI GAGTC 4 cut(s) 235, 2120, 2591, 4764
ScrFI CCNGG 6 cut(s) 404, 622, 971, 1292, 1855, 2240
SduI GDGCHC 4 cut(s) 2810, 2943, 3648, 4392
SfcI CTRYAG 4 cut(s) 340, 1760, 2119, 2143
Sfr274I CTCGAG 1 cut(s) 1591
SinI GGWCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
SlaI CTCGAG 1 cut(s) 1591
SmiMI CAYNNNNRTG 6 cut(s) 2457, 2948, 3552, 3653, 4296, 4397
SpeI ACTAGT 3 cut(s) 2981, 3686, 4430
SphI GCATGC 1 cut(s) 2059
SseBI AGGCCT 2 cut(s) 233, 1673
SspI AATATT 3 cut(s) 628, 1724, 2223
SspMI CTAG 5 cut(s) 320, 1871, 2982, 3687, 4431
StuI AGGCCT 2 cut(s) 233, 1673
StyD4I CCNGG 6 cut(s) 402, 620, 969, 1290, 1853, 2238
StyI CCWWGG 6 cut(s) 943, 975, 2018, 2762, 3018, 3723
TaaI ACNGT 3 cut(s) 586, 834, 2459
TaiI ACGT 8 cut(s) 654, 1012, 1066, 1233, 1422, 1962, 2166, 2853
TaqII GACCGA 1 cut(s) 4436
TatI WGTACW 4 cut(s) 899, 1169, 1304, 4579
TfiI GAWTC 5 cut(s) 785, 1195, 1358, 1701, 3002
TseFI GTSAC 5 cut(s) 739, 994, 1037, 1181, 1906
TseI GCWGC 5 cut(s) 646, 1105, 1599, 2497, 4675
Tsp45I GTSAC 5 cut(s) 739, 994, 1037, 1181, 1906
TspGWI ACGGA 3 cut(s) 1141, 1511, 2804
Tth111I GACNNNGTC 1 cut(s) 1513
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 199
VpaK11BI GGWCC 7 cut(s) 598, 1515, 1777, 2044, 2993, 3675, 4419
VspI ATTAAT 3 cut(s) 1115, 1472, 3011
XagI CCTNNNNNAGG 2 cut(s) 974, 2816
XceI RCATGY 1 cut(s) 2059
XhoI CTCGAG 1 cut(s) 1591
XmiI GTMKAC 3 cut(s) 1335, 2142, 2889
XmnI GAANNNNTTC 5 cut(s) 2330, 3048, 3138, 3753, 4497
XspI CTAG 5 cut(s) 320, 1871, 2982, 3687, 4431
ZraI GACGTC 2 cut(s) 1010, 2851
ZrmI AGTACT 2 cut(s) 1171, 1306
Zsp2I ATGCAT 2 cut(s) 2061, 4768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.