Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0002268

Physical Location & Seq

Genomic Coordinates Forward (+)

23727093 .. 23733707

Gene Structure 6615 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0254600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2619 bp

ATGGTGGAAGGTAGGGTTTGTTTGTCCAAAGAGGCCCAAAGTGGATTCGAATTTCTGAAGCGAAGAAGGCTTCAGCATATGAGATCAGAAACTATTCCTGAGCCTGTAAGTTTCACGAATATGATGTCTAGAAGTGGAGGGGATGCTTTAAAAGCTTCAGCATCCTGTGGTGTTAGAATACGTGGAGGTGCCGATTCAGTTGCTCGGTCCAATGGTGCTCCACAGGGGAAAGATGTTTTTTCAAAGCGTAAGGTGGATAAATTTGAAACAAGTGATCTAGACTGGACCGAGAAAATTCCCGAGTGTCCTGTATACTATCCAGCAAAGGAGGAGTTTGAGGATCCTTTAGTTTATTTACAGAAAATAGCTCCAGAAGCTTCCAAATATGGTATATGCAAGATTATCTCCCCTGTTAGTGCTTCTACTCCTGCTGGGGTTGTATTGATGAGGGAAAAAGCAGGGTTTAAGTTCACTACTAGAGTACAACCTCTTCGCCTTGCTGAGTGGGACAATGATGACAGGGTCACTTTTTTCATGAGCGGAAGAAACTATACATTCCGTGATTTCGAGAAAATGGCGAACAAGGTTTTTGCTCGTAGATACTGTAGTGCTGGGTCTCTTCCTCCTACGTACTTGGAAAAAGAATTTTGGAAAGAAATTAGTTCTGGAAAGACAGAAAGTGTTGAATATGCCTGTGATGTAGATGGTAGTGCATTTTCATGTTCTCCCAATGATCCACTTGGAAGTAGTAAATGGAATTTAAAGAATCTTTCAAGGCTACCCAAGTCTATTCTGCGTCTTCTGGAAAACGCAATTCCGGGTGTAACAGATCCTATGCTATACATTGGAATGATATTTAGCATGTTTGCTTGGCATGTGGAAGATCACTATTTGTACAGTATCAATTATCATCATTGTGGCGCATCCAAAACCTGGTATGGCATTCCAGGTCATGCCGCCCTGCAATTTGAAAAGGTTGTCAAGGAACATGTTTACACCAATGATATTGTACCAAGTGATGGAGAGGATGGAGCTTTTGATGTCTTATTGGGGAAAACAACTCTGTTTCCTCCCAGCATATTGTTGGAACATGATGTACCTGTCTACAAAGCTGTGCAAAAGCCAGGAGAGTTTGTGGTTACTTTCCCTAGAGCGTATCATGCTGGATTCAGTCATGGTTTCAATTGTGGTGAGGCAGTCAACTTTGCTATTGGTGATTGGTTCCCATTGGGTGCTATTGCTAGCCGGCGTTATGCACTTCTTAATAGGATGCCTCTGCTTCCTCATGAAGAACTCCTGTGCAAAGAAGCCATGCTTCTCCATACAAGTTTAGAGCTTGAAGATTCAGATTATTCTTCTGCTGACTTGGTTTCCCATCATCGCATTAAGGTCTCTTTTGTGAAACTGATGCGGTTTCAGCATCGTGCTCGTTGGACTTTAATGAAGTCTGGGGCATGCACTGGTGTCTTGCCAAACTCTTATGGAACTGTTCTCTGCAGCATATGCAAGCGCGATTGCTATCTTGCCTACATTAGTTGCAACTGTTATATGCATCATGTGTGCCTTCGCCATGATGTAAGGTCATTGGACTTGTCATGTCAGAGCAATCCTACTCTCTTTTTAAGGGAGGAGATAGCAGAAATGGAAGCTGCAGCAAGTAAATTTGAAGAGGAAGCGGAAGTGATAGAGGAGATTCAAGGTCAAGGTGAAAATGGTGATGACTTGTATTCATATCCATTAATTTTGTCTCCGATAGCTGAAGAGAAAGGGTACTCCCCTTACTGCAATATAAAATTTGAGTTGAACCCCGAACTCCCTGGTACAACAGAAATCACTGGCACAACTCTGGCTCAGTCACAAGAGCCAGCTGCACTTGGTTCTCATACCAGTCCTGCAGTGTCAGAAGGTTCCATTTCATGTATTGCATCTACTCTTTGTTCTCTTTCAGAGCCACTTGAGAGCTTGTATGCACCCAATAATGGAATTGCCAACTCTAAGACAGTGACTCTAAATTCCAAAAGGTCTGCATATGAGTCTTCTCGGTCCTCCCCATCATATGATGAATGCTCAAGTGTATGTCCAGGAAGTTCTAACGCATTGGAGGTTAGGCCTTTAGTAGATCAAGGCAGTGATGATTCTGATTCAGAGATATTTAGGGTTAAGCGCCGTTCTTCATTGAAGGTGGAGAGAAGAATTATAAATGATGTCTTGCCATCAAATCATTCTGAGAACCAGCAGGGATTTAAGCGACTGAAGAAACTGCAACCTGATCCTAGATGTGGGCGGTCGGTGCCATTACAAAGCTGCAGCACTACGGAATCAAGTACTAAGTTGGTTACAACTACTAATAGCAAAGGGGCTCCGGAGAATGCTTTAAGAGATGACAGGTTTGCCAGAGGAAGTACCATATCTAGAGGAAGTACCATTCCCTTCTCTATCAAGTTTAAGAAGTTGACCAATGAAGATTCTGTTAGGAGACAGCGAGATCACCACAGAACAGATATACGCCAGCTTGAGTTGGGAAAGAGCCGCAGGGAACCTCCCCCAACAGAGATTGGGCCAAAGCGCCTTAAGGTCAAAGGCCCTTCTTTCTTAGCTTCGGAGAACAGGTTAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

872

Amino Acids

97.27

Weight (kDa)

7.26

Isoelectric Point (pI)

57.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjN	PF02375	103 - 135	1.3e-14	jmjN domain
JmjC	PF02373	279 - 402	2.2e-39	JmjC domain, hydroxylase
zf-C5HC2	PF02928	499 - 550	2.4e-09	C5HC2 zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002268)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g19150 FvH4_4g19150 FvH4_4g19150 FvH4_4g19150 FvH4_4g19150 FvH4_4g19150 FvH4_4g19150
prunus_persica	Prupe.4G109500_v2.0.a1 Prupe.4G109500_v2.0.a1 Prupe.4G109500_v2.0.a1 Prupe.4G109600_v2.0.a1 Prupe.4G109900_v2.0.a1 Prupe.4G263700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0424091 RchiOBHm_Chr5g0019831 RchiOBHm_Chr5g0020211 RchiOBHm_Chr5g0020221
rosa_laevigata	RLG00000007504
rosa_multiflora	Rmu_co8478429.1_g000001 Rmu_sc0002260.1_g000052 Rmu_sc0002260.1_g000058 Rmu_sc0002260.1_g000060
rosa_roxburghii	Rroxscaffold_5G00366100
rosa_rugosa	Rorug04G0192900 Rorug04G0193000 Rorug04G0193100 Rorug04G0193100 Rorug04G0193100 Rorug07G0254600
rosa_samantha	Rh4AG251200 Rh4BG256200 Rh4BG256300 Rh4CG266700 Rh4DG250800 Rh4DG250900 Rh5AG148300 Rh5AG366200 Rh5AG366300 Rh5CG159200
rosa_wichuraiana	Rw4G021640 Rw5G013110 Rw5G013120 Rw5G034340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2198
AccB1I	GGYRCC	2 cut(s)	188, 2290
AccB7I	CCANNNNNTGG	2 cut(s)	933, 1019
AccBSI	CCGCTC	1 cut(s)	540
AccI	GTMKAC	2 cut(s)	312, 1104
AccII	CGCG	1 cut(s)	1512
AccIII	TCCGGA	1 cut(s)	2362
AciI	CCGC	6 cut(s)	540, 957, 1411, 1676, 2284, 2530
AclWI	GGATC	5 cut(s)	335, 348, 728, 824, 2264
AcsI	RAATTY	8 cut(s)	50, 260, 294, 644, 757, 1661, 1793, 2011
AcuI	CTGAAG	5 cut(s)	56, 77, 141, 1779, 2273
AfiI	CCNNNNNNNGG	4 cut(s)	933, 1019, 1979, 2279
AflII	CTTAAG	1 cut(s)	2570
AflIII	ACRYGT	1 cut(s)	988
AjnI	CCWGG	5 cut(s)	932, 946, 1123, 1816, 2080
Alw21I	GWGCWC	2 cut(s)	220, 1429
Alw26I	GTCTC	4 cut(s)	621, 1396, 1752, 2470
AlwI	GGATC	5 cut(s)	335, 348, 728, 824, 2264
Ama87I	CYCGRG	1 cut(s)	299
Aor13HI	TCCGGA	1 cut(s)	2362
AoxI	GGCC	4 cut(s)	33, 2108, 2558, 2581
ApeKI	GCWGC	6 cut(s)	1497, 1649, 1652, 1868, 2304, 2307
ApoI	RAATTY	8 cut(s)	50, 260, 294, 644, 757, 1661, 1793, 2011
ArsI	GACNNNNNNTTYG	2 cut(s)	110, 142
AseI	ATTAAT	1 cut(s)	1739
Asp700I	GAANNNNTTC	1 cut(s)	93
AspLEI	GCGC	4 cut(s)	923, 1512, 2166, 2568
AspS9I	GGNCC	6 cut(s)	34, 207, 285, 2043, 2558, 2582
AsuC2I	CCSGG	1 cut(s)	819
AsuHPI	GGTGA	5 cut(s)	1202, 1226, 1718, 1727, 2480
AsuII	TTCGAA	1 cut(s)	48
AsuNHI	GCTAGC	1 cut(s)	1241
AvaI	CYCGRG	1 cut(s)	299
AvaII	GGWCC	3 cut(s)	207, 285, 2043
BaeI	ACNNNNGTAYC	2 cut(s)	592, 625
BamHI	GGATCC	1 cut(s)	340
BanI	GGYRCC	2 cut(s)	188, 2290
BanII	GRGCYC	1 cut(s)	2362
BarI	GAAGNNNNNNTAC	4 cut(s)	474, 506, 535, 567
BbsI	GAAGAC	2 cut(s)	791, 2028
Bbv12I	GWGCWC	2 cut(s)	220, 1429
BbvI	GCAGC	6 cut(s)	1509, 1636, 1664, 1855, 2291, 2319
BccI	CCATC	6 cut(s)	698, 1013, 1022, 1383, 2059, 2221
BceAI	ACGGC	1 cut(s)	2151
BcgI	CGANNNNNNTGC	4 cut(s)	182, 216, 1409, 1443
BciT130I	CCWGG	5 cut(s)	934, 948, 1125, 1818, 2082
BcnI	CCSGG	1 cut(s)	819
BcoDI	GTCTC	4 cut(s)	621, 1396, 1752, 2470
BfaI	CTAG	7 cut(s)	129, 278, 477, 1149, 1242, 2274, 2412
BfmI	CTRYAG	5 cut(s)	604, 1495, 1650, 1893, 2305
BfoI	RGCGCY	2 cut(s)	2167, 2569
BfrI	CTTAAG	1 cut(s)	2570
BisI	GCNGC	8 cut(s)	957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
BlsI	GCNGC	8 cut(s)	958, 1499, 1651, 1654, 1870, 2306, 2309, 2531
BmcAI	AGTACT	1 cut(s)	2326
Bme1390I	CCNGG	6 cut(s)	819, 934, 948, 1125, 1818, 2082
Bme18I	GGWCC	3 cut(s)	207, 285, 2043
BmeT110I	CYCGRG	1 cut(s)	299
BmgT120I	GGNCC	6 cut(s)	34, 207, 285, 2043, 2558, 2582
BmiI	GGNNCC	7 cut(s)	190, 342, 1223, 1909, 2292, 2361, 2538
BmrFI	CCNGG	6 cut(s)	819, 934, 948, 1125, 1818, 2082
BmsI	GCATC	8 cut(s)	133, 170, 932, 1260, 1398, 1429, 1561, 1934
BmtI	GCTAGC	1 cut(s)	1245
BpiI	GAAGAC	2 cut(s)	791, 2028
BpmI	CTGGAG	1 cut(s)	354
Bpu10I	CCTNAGC	1 cut(s)	99
Bpu14I	TTCGAA	1 cut(s)	48
BpuEI	CTTGAG	3 cut(s)	1976, 2053, 2534
BpuMI	CCSGG	1 cut(s)	819
BsaAI	YACGTR	2 cut(s)	182, 630
BsaBI	GATNNNNATC	1 cut(s)	1518
BsaI	GGTCTC	2 cut(s)	621, 1396
BsaJI	CCNNGG	1 cut(s)	1816
BsaWI	WCCGGW	1 cut(s)	2362
BsaXI	ACNNNNNCTCC	3 cut(s)	1119, 1149, 2594
Bsc4I	CCNNNNNNNGG	4 cut(s)	933, 1019, 1979, 2279
Bse118I	RCCGGY	1 cut(s)	1245
Bse1I	ACTGG	4 cut(s)	287, 1465, 1840, 1887
Bse8I	GATNNNNATC	1 cut(s)	1518
BseAI	TCCGGA	1 cut(s)	2362
BseBI	CCWGG	5 cut(s)	934, 948, 1125, 1818, 2082
BseDI	CCNNGG	1 cut(s)	1816
BseGI	GGATG	5 cut(s)	148, 161, 923, 1033, 1275
BseJI	GATNNNNATC	1 cut(s)	1518
BseLI	CCNNNNNNNGG	4 cut(s)	933, 1019, 1979, 2279
BseMII	CTCAG	4 cut(s)	90, 492, 1865, 2217
BseNI	ACTGG	4 cut(s)	287, 1465, 1840, 1887
BseRI	GAGGAG	3 cut(s)	344, 1643, 1703
BseXI	GCAGC	6 cut(s)	1509, 1636, 1664, 1855, 2291, 2319
BseYI	CCCAGC	3 cut(s)	431, 611, 1073
BsgI	GTGCAG	1 cut(s)	1854
Bsh1236I	CGCG	1 cut(s)	1512
Bsh1285I	CGRYCG	1 cut(s)	2288
BshFI	GGCC	4 cut(s)	35, 2110, 2560, 2583
BshNI	GGYRCC	2 cut(s)	188, 2290
BsiEI	CGRYCG	1 cut(s)	2288
BsiHKAI	GWGCWC	2 cut(s)	220, 1429
BsiHKCI	CYCGRG	1 cut(s)	299
BsiSI	CCGG	3 cut(s)	818, 1246, 2363
BslFI	GGGAC	1 cut(s)	521
BslI	CCNNNNNNNGG	4 cut(s)	933, 1019, 1979, 2279
BsmAI	GTCTC	4 cut(s)	621, 1396, 1752, 2470
BsmFI	GGGAC	1 cut(s)	521
BsmI	GAATGC	3 cut(s)	942, 2069, 2374
BsnI	GGCC	4 cut(s)	35, 2110, 2560, 2583
Bso31I	GGTCTC	2 cut(s)	621, 1396
BsoBI	CYCGRG	1 cut(s)	299
Bsp119I	TTCGAA	1 cut(s)	48
Bsp1286I	GDGCHC	3 cut(s)	220, 1429, 2362
Bsp13I	TCCGGA	1 cut(s)	2362
Bsp1407I	TGTACA	1 cut(s)	894
Bsp143I	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BspACI	CCGC	6 cut(s)	540, 957, 1411, 1676, 2284, 2530
BspANI	GGCC	4 cut(s)	35, 2110, 2560, 2583
BspCNI	CTCAG	4 cut(s)	91, 493, 1864, 2218
BspEI	TCCGGA	1 cut(s)	2362
BspFNI	CGCG	1 cut(s)	1512
BspHI	TCATGA	2 cut(s)	534, 1285
BspLI	GGNNCC	7 cut(s)	190, 342, 1223, 1909, 2292, 2361, 2538
BspMAI	CTGCAG	4 cut(s)	1499, 1654, 1897, 2309
BspOI	GCTAGC	1 cut(s)	1245
BspPI	GGATC	5 cut(s)	335, 348, 728, 824, 2264
BspT104I	TTCGAA	1 cut(s)	48
BspT107I	GGYRCC	2 cut(s)	188, 2290
BspTI	CTTAAG	1 cut(s)	2570
BspTNI	GGTCTC	2 cut(s)	621, 1396
BsrBI	CCGCTC	1 cut(s)	540
BsrFI	RCCGGY	1 cut(s)	1245
BsrGI	TGTACA	1 cut(s)	894
BsrI	ACTGG	4 cut(s)	287, 1465, 1840, 1887
BssAI	RCCGGY	1 cut(s)	1245
BssECI	CCNNGG	1 cut(s)	1816
BssMI	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BssNAI	GTATAC	1 cut(s)	313
Bst1107I	GTATAC	1 cut(s)	313
Bst2UI	CCWGG	5 cut(s)	934, 948, 1125, 1818, 2082
Bst4CI	ACNGT	5 cut(s)	605, 899, 1489, 1544, 2002
Bst6I	CTCTTC	4 cut(s)	495, 624, 1662, 1755
BstAFI	CTTAAG	1 cut(s)	2570
BstAPI	GCANNNNNTGC	1 cut(s)	1503
BstAUI	TGTACA	1 cut(s)	894
BstBAI	YACGTR	2 cut(s)	182, 630
BstBI	TTCGAA	1 cut(s)	48
BstC8I	GCNNGC	6 cut(s)	1243, 1247, 1456, 1508, 1866, 2510
BstDEI	CTNAG	7 cut(s)	99, 501, 1851, 1995, 2226, 2328, 2593
BstF5I	GGATG	5 cut(s)	148, 161, 923, 1033, 1275
BstFNI	CGCG	1 cut(s)	1512
BstH2I	RGCGCY	2 cut(s)	2167, 2569
BstHHI	GCGC	4 cut(s)	923, 1512, 2166, 2568
BstKTI	GATC	9 cut(s)	86, 277, 343, 736, 832, 886, 2122, 2272, 2488
BstMAI	GTCTC	4 cut(s)	621, 1396, 1752, 2470
BstMBI	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BstMCI	CGRYCG	1 cut(s)	2288
BstMWI	GCNNNNNNNGC	7 cut(s)	67, 152, 374, 1160, 1417, 1503, 2290
BstNI	CCWGG	5 cut(s)	934, 948, 1125, 1818, 2082
BstNSI	RCATGY	4 cut(s)	865, 878, 992, 1458
BstSCI	CCNGG	6 cut(s)	817, 932, 946, 1123, 1816, 2080
BstSFI	CTRYAG	5 cut(s)	604, 1495, 1650, 1893, 2305
BstSNI	TACGTA	1 cut(s)	630
BstUI	CGCG	1 cut(s)	1512
BstV1I	GCAGC	6 cut(s)	1509, 1636, 1664, 1855, 2291, 2319
BstV2I	GAAGAC	2 cut(s)	791, 2028
BstX2I	RGATCY	2 cut(s)	340, 829
BstYI	RGATCY	2 cut(s)	340, 829
BstZ17I	GTATAC	1 cut(s)	313
BsuRI	GGCC	4 cut(s)	35, 2110, 2560, 2583
BtgZI	GCGATG	1 cut(s)	1364
BtsCI	GGATG	5 cut(s)	148, 161, 923, 1033, 1275
BtsI	GCAGTG	2 cut(s)	1902, 2134
BtsIMutI	CAGTG	5 cut(s)	1458, 1833, 1902, 2007, 2134
Cac8I	GCNNGC	6 cut(s)	1243, 1247, 1456, 1508, 1866, 2510
CciI	TCATGA	2 cut(s)	534, 1285
CfoI	GCGC	4 cut(s)	923, 1512, 2166, 2568
Cfr10I	RCCGGY	1 cut(s)	1245
Cfr13I	GGNCC	6 cut(s)	34, 207, 285, 2043, 2558, 2582
CseI	GACGC	1 cut(s)	785
CsiI	ACCWGGT	1 cut(s)	932
DdeI	CTNAG	7 cut(s)	99, 501, 1851, 1995, 2226, 2328, 2593
DpnI	GATC	9 cut(s)	85, 276, 342, 735, 831, 885, 2121, 2271, 2487
DpnII	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
DraI	TTTAAA	2 cut(s)	150, 762
Eam1104I	CTCTTC	4 cut(s)	495, 624, 1662, 1755
EarI	CTCTTC	4 cut(s)	495, 624, 1662, 1755
Eco105I	TACGTA	1 cut(s)	630
Eco147I	AGGCCT	1 cut(s)	2110
Eco24I	GRGCYC	1 cut(s)	2362
Eco31I	GGTCTC	2 cut(s)	621, 1396
Eco47I	GGWCC	3 cut(s)	207, 285, 2043
Eco57I	CTGAAG	5 cut(s)	56, 77, 141, 1779, 2273
Eco88I	CYCGRG	1 cut(s)	299
EcoO109I	RGGNCCY	1 cut(s)	2582
EcoRII	CCWGG	5 cut(s)	932, 946, 1123, 1816, 2080
EcoT22I	ATGCAT	1 cut(s)	1554
EcoT38I	GRGCYC	1 cut(s)	2362
FalI	AAGNNNNNCTT	2 cut(s)	1299, 1331
FaqI	GGGAC	1 cut(s)	521
FauNDI	CATATG	4 cut(s)	78, 1502, 2029, 2056
FblI	GTMKAC	2 cut(s)	312, 1104
Fnu4HI	GCNGC	8 cut(s)	957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
FokI	GGATG	5 cut(s)	148, 155, 910, 1040, 1282
FriOI	GRGCYC	1 cut(s)	2362
Fsp4HI	GCNGC	8 cut(s)	957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
FspBI	CTAG	7 cut(s)	129, 278, 477, 1149, 1242, 2274, 2412
GlaI	GCGC	4 cut(s)	922, 1511, 2165, 2567
GluI	GCNGC	8 cut(s)	957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
GsaI	CCCAGC	3 cut(s)	435, 615, 1077
GsuI	CTGGAG	1 cut(s)	354
HaeII	RGCGCY	2 cut(s)	2167, 2569
HaeIII	GGCC	4 cut(s)	35, 2110, 2560, 2583
HapII	CCGG	3 cut(s)	818, 1246, 2363
HgaI	GACGC	1 cut(s)	785
HhaI	GCGC	4 cut(s)	923, 1512, 2166, 2568
Hin6I	GCGC	4 cut(s)	921, 1510, 2164, 2566
HinP1I	GCGC	4 cut(s)	921, 1510, 2164, 2566
HincII	GTYRAC	2 cut(s)	1201, 2454
HindII	GTYRAC	2 cut(s)	1201, 2454
HindIII	AAGCTT	2 cut(s)	153, 375
HpaII	CCGG	3 cut(s)	818, 1246, 2363
HphI	GGTGA	5 cut(s)	1202, 1226, 1718, 1727, 2480
Hpy166II	GTNNAC	6 cut(s)	313, 471, 994, 1105, 1201, 2454
Hpy8I	GTNNAC	6 cut(s)	313, 471, 994, 1105, 1201, 2454
HpyAV	CCTTC	6 cut(s)	60, 1574, 1898, 2173, 2440, 2595
HpyCH4III	ACNGT	5 cut(s)	605, 899, 1489, 1544, 2002
HpyCH4IV	ACGT	2 cut(s)	181, 629
HpyF10VI	GCNNNNNNNGC	7 cut(s)	67, 152, 374, 1160, 1417, 1503, 2290
HpyF3I	CTNAG	7 cut(s)	99, 501, 1851, 1995, 2226, 2328, 2593
HpySE526I	ACGT	2 cut(s)	181, 629
HspAI	GCGC	4 cut(s)	921, 1510, 2164, 2566
Kpn2I	TCCGGA	1 cut(s)	2362
KroI	GCCGGC	1 cut(s)	1245
KroNI	GCCGGC	1 cut(s)	1247
Kzo9I	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
LmnI	GCTCC	4 cut(s)	223, 373, 1031, 2365
Lsp1109I	GCAGC	6 cut(s)	1509, 1636, 1664, 1855, 2291, 2319
LweI	GCATC	8 cut(s)	133, 170, 932, 1260, 1398, 1429, 1561, 1934
MabI	ACCWGGT	1 cut(s)	932
MaeI	CTAG	7 cut(s)	129, 278, 477, 1149, 1242, 2274, 2412
MaeII	ACGT	2 cut(s)	181, 629
MaeIII	GTNAC	6 cut(s)	523, 823, 1138, 1854, 2002, 2335
MalI	GATC	9 cut(s)	85, 276, 342, 735, 831, 885, 2121, 2271, 2487
MbiI	CCGCTC	1 cut(s)	540
MboI	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
MfeI	CAATTG	1 cut(s)	1183
MflI	RGATCY	2 cut(s)	340, 829
MhlI	GDGCHC	3 cut(s)	220, 1429, 2362
MlyI	GAGTC	2 cut(s)	1999, 2042
MmeI	TCCRAC	2 cut(s)	1065, 1412
Mph1103I	ATGCAT	1 cut(s)	1554
MroI	TCCGGA	1 cut(s)	2362
MroNI	GCCGGC	1 cut(s)	1245
MroXI	GAANNNNTTC	1 cut(s)	93
MslI	CAYNNNNRTG	4 cut(s)	119, 718, 848, 915
MspA1I	CMGCKG	1 cut(s)	1868
MspCI	CTTAAG	1 cut(s)	2570
MspI	CCGG	3 cut(s)	818, 1246, 2363
MspR9I	CCNGG	6 cut(s)	819, 934, 948, 1125, 1818, 2082
MunI	CAATTG	1 cut(s)	1183
Mva1269I	GAATGC	3 cut(s)	942, 2069, 2374
MvaI	CCWGG	5 cut(s)	934, 948, 1125, 1818, 2082
MvnI	CGCG	1 cut(s)	1512
MwoI	GCNNNNNNNGC	7 cut(s)	67, 152, 374, 1160, 1417, 1503, 2290
NaeI	GCCGGC	1 cut(s)	1247
NciI	CCSGG	1 cut(s)	819
NdeI	CATATG	4 cut(s)	78, 1502, 2029, 2056
NdeII	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
NgoMIV	GCCGGC	1 cut(s)	1245
NheI	GCTAGC	1 cut(s)	1241
NlaIV	GGNNCC	7 cut(s)	190, 342, 1223, 1909, 2292, 2361, 2538
NmuCI	GTSAC	3 cut(s)	523, 1854, 2002
NsiI	ATGCAT	1 cut(s)	1554
NspI	RCATGY	4 cut(s)	865, 878, 992, 1458
NspV	TTCGAA	1 cut(s)	48
PaeI	GCATGC	1 cut(s)	1458
PagI	TCATGA	2 cut(s)	534, 1285
PceI	AGGCCT	1 cut(s)	2110
PciI	ACATGT	1 cut(s)	988
PctI	GAATGC	3 cut(s)	942, 2069, 2374
PdiI	GCCGGC	1 cut(s)	1247
PdmI	GAANNNNTTC	1 cut(s)	93
PflFI	GACNNNGTC	1 cut(s)	521
PflMI	CCANNNNNTGG	2 cut(s)	933, 1019
PfoI	TCCNGGA	1 cut(s)	2080
PkrI	GCNGC	8 cut(s)	958, 1499, 1651, 1654, 1870, 2306, 2309, 2531
PleI	GAGTC	2 cut(s)	1999, 2041
PpsI	GAGTC	2 cut(s)	1999, 2041
Ppu21I	YACGTR	2 cut(s)	182, 630
PscI	ACATGT	1 cut(s)	988
PshBI	ATTAAT	1 cut(s)	1739
PsiI	TTATAA	1 cut(s)	2198
Psp6I	CCWGG	5 cut(s)	932, 946, 1123, 1816, 2080
PspFI	CCCAGC	3 cut(s)	431, 611, 1073
PspGI	CCWGG	5 cut(s)	932, 946, 1123, 1816, 2080
PspN4I	GGNNCC	7 cut(s)	190, 342, 1223, 1909, 2292, 2361, 2538
PspPI	GGNCC	6 cut(s)	34, 207, 285, 2043, 2558, 2582
PsrI	GAACNNNNNNTAC	2 cut(s)	1080, 1112
PstI	CTGCAG	4 cut(s)	1499, 1654, 1897, 2309
PsuI	RGATCY	2 cut(s)	340, 829
PsyI	GACNNNGTC	1 cut(s)	521
PvuII	CAGCTG	1 cut(s)	1868
RseI	CAYNNNNRTG	4 cut(s)	119, 718, 848, 915
SatI	GCNGC	8 cut(s)	957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
Sau3AI	GATC	9 cut(s)	83, 274, 340, 733, 829, 883, 2119, 2269, 2485
Sau96I	GGNCC	6 cut(s)	34, 207, 285, 2043, 2558, 2582
ScaI	AGTACT	1 cut(s)	2326
SchI	GAGTC	2 cut(s)	1999, 2042
ScrFI	CCNGG	6 cut(s)	819, 934, 948, 1125, 1818, 2082
SduI	GDGCHC	3 cut(s)	220, 1429, 2362
SexAI	ACCWGGT	1 cut(s)	932
SfaNI	GCATC	8 cut(s)	133, 170, 932, 1260, 1398, 1429, 1561, 1934
SfcI	CTRYAG	5 cut(s)	604, 1495, 1650, 1893, 2305
SfuI	TTCGAA	1 cut(s)	48
SinI	GGWCC	3 cut(s)	207, 285, 2043
SmiMI	CAYNNNNRTG	4 cut(s)	119, 718, 848, 915
SmlI	CTYRAG	4 cut(s)	1955, 2068, 2513, 2570
SmoI	CTYRAG	4 cut(s)	1955, 2068, 2513, 2570
SnaBI	TACGTA	1 cut(s)	630
SphI	GCATGC	1 cut(s)	1458
SseBI	AGGCCT	1 cut(s)	2110
SsiI	CCGC	6 cut(s)	540, 957, 1411, 1676, 2284, 2530
SspMI	CTAG	7 cut(s)	129, 278, 477, 1149, 1242, 2274, 2412
StuI	AGGCCT	1 cut(s)	2110
StyD4I	CCNGG	6 cut(s)	817, 932, 946, 1123, 1816, 2080
TaaI	ACNGT	5 cut(s)	605, 899, 1489, 1544, 2002
TaiI	ACGT	2 cut(s)	184, 632
TaqI	TCGA	2 cut(s)	48, 567
TaqII	GACCGA	3 cut(s)	195, 302, 2031
TatI	WGTACW	3 cut(s)	481, 894, 2324
TauI	GCSGC	2 cut(s)	959, 2532
TscAI	CASTG	5 cut(s)	1465, 1840, 1902, 2007, 2134
TseFI	GTSAC	3 cut(s)	523, 1854, 2002
TseI	GCWGC	6 cut(s)	1497, 1649, 1652, 1868, 2304, 2307
Tsp45I	GTSAC	3 cut(s)	523, 1854, 2002
TspDTI	ATGAA	9 cut(s)	523, 708, 1302, 1457, 1719, 1905, 2076, 2163, 2475
TspGWI	ACGGA	2 cut(s)	548, 2330
TspRI	CASTG	5 cut(s)	1465, 1840, 1902, 2007, 2134
Tth111I	GACNNNGTC	1 cut(s)	521
Van91I	CCANNNNNTGG	2 cut(s)	933, 1019
Vha464I	CTTAAG	1 cut(s)	2570
VpaK11BI	GGWCC	3 cut(s)	207, 285, 2043
VspI	ATTAAT	1 cut(s)	1739
XapI	RAATTY	8 cut(s)	50, 260, 294, 644, 757, 1661, 1793, 2011
XbaI	TCTAGA	3 cut(s)	128, 277, 2411
XceI	RCATGY	4 cut(s)	865, 878, 992, 1458
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1081
XmiI	GTMKAC	2 cut(s)	312, 1104
XmnI	GAANNNNTTC	1 cut(s)	93
XspI	CTAG	7 cut(s)	129, 278, 477, 1149, 1242, 2274, 2412
ZrmI	AGTACT	1 cut(s)	2326
Zsp2I	ATGCAT	1 cut(s)	1554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.