pycom08g13240

Flotillin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000793
Physical Location & Seq
Forward (+)
11867771 .. 11870130
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13240.2

Sequence Viewer

Length: 1440 bp
ATGTATAAAGTAGCAAACGCTTCAGAGTATCTGGTGATCACCGGGATCGGAATCACCGACATAAAGCTTGCAAAGAAGGCATGGATCCTCCCCGGCCAGTCCTTCATAATCTTCGACGTGTCCCCCGTCAACTATACCTTCGAAGTCCAAGCCATGAGCTCGGAAAAGCTCCCCTTCATGCTGCCCGCCGTCTTCACCATCGGCCCACGGATCGATGACATCCAGAGTCTCCAAAAGTACGCCAAGCTCATTTCCCGCCATGACAAGCTCTCTAGCCACGTGAAAGAGCTTGTTCAGGGGATCATTGAGGGCGAGACTCGTGTTCTCGCGGCCTCGATGACCATGGAGGAGGTGTTTAAGGGGACAAAAGAGTTTAAGCAAGAGGTGTTTGAGAAGGTTCAGTTGGAACTCAATCAGTTTGGGCTTTTGATTTATAATGCAAATGTCAAGCAGTTGGTGGATGTGCCGGGGCATGAGTATTTTAGTTACTTGGGGCAGAAAACACAGATGGAGGCGGCAAATCAGGCAAGGGTTGACGTGGCGGAGGCAAGAAAGAAGGGGGAGATAGGGTCGAAGATGAGGGAGGGCGATACGCTGCAGTATGCGGCCAAGATTGACGCAGAGACGAAGATATATGGGACGAAGAGGGAGGGGGAGGGGAGGATGGCGGAGATTAAGGTGAAGGCGGAGGTCAAGGTGTATGAGAATGTGAGGGAGGCTGAGGTGGCTGAGGCAAATGCTGAGCTGGCCAAGAAGAAGGCCGGGTGGGCGAAAGAGGCGCAGGTGGCGGAGCTGGAGGCCGCAAAGGCTGTGGCATTGAGGGATGCCGAGTTGCAAACGGAGGTTGAGAGGATGAATGCCTTGACTAGGACCGAGAAGCTAAAGGCTGAGTTCTTGAGCAAGGCTAGCGTTGAGTATGAAACAAAGGTACAAGAGGCAGATTGGGAGTTATACAAAAAACAACAAGCCTCAGAGGCAATTCGTTTCCAAAGGGAGAAAGAGGCAGAGGCCAAAAAGGCATTGTCGGAGGCAGAATTCTATGCACGTCAACAAGTTGCCGACGGAGAATTTTATGCAAAGAAAAAAGAGGCTGAGGGACTCATGGCCCTTGGACAAGCTCAAGGTGCATATCTACGCCACCTTTTGGATGCAGTTGGAGGAAACTATGCAGCCATGAGAGACTTTATGATGATCAACAGTGGCATGTTTCAAGAAATTGCTAAGATCAATGCCGAGGCCATGCGCGGACTTCAGCCGAAAATCAGCATTTGGACAAATGGCGGGGGAGAGGGTGGTGATGGTGGTGCAAATGGGGCAATGAAGGAGGTTGCCGGAGTTTACAAGATGTTGCCACCATTGTTCAAGACGGTTCATGAACAAACCGGCATGTCGCCTCCGGCTTGGATGGGCACTTTGGAAAATTCCCACTCTAGCAACTAA

Protein Analysis

480

Amino Acids

53.25

Weight (kDa)

5.93

Isoelectric Point (pI)

31.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
AarI CACCTGC 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 772
AccB7I CCANNNNNTGG 1 cut(s) 1144
AccII CGCG 2 cut(s) 329, 1245
AclWI GGATC 5 cut(s) 53, 79, 92, 218, 308
AcoI YGGCCR 3 cut(s) 94, 606, 747
AcsI RAATTY 3 cut(s) 1034, 1067, 1420
AcuI CTGAAG 2 cut(s) 6, 1235
AcvI CACGTG 1 cut(s) 280
AfaI GTAC 2 cut(s) 239, 930
AfiI CCNNNNNNNGG 2 cut(s) 867, 1144
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 2 cut(s) 1211, 1363
AjiI CACGTC 3 cut(s) 118, 538, 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 4 cut(s) 233, 308, 617, 1173
AlwI GGATC 5 cut(s) 53, 79, 92, 218, 308
ApeKI GCWGC 3 cut(s) 181, 595, 1169
ApoI RAATTY 3 cut(s) 1034, 1067, 1420
AspLEI GCGC 2 cut(s) 781, 1245
AspS9I GGNCC 3 cut(s) 203, 870, 1105
AsuC2I CCSGG 4 cut(s) 43, 93, 468, 763
AsuHPI GGTGA 6 cut(s) 31, 46, 46, 187, 691, 1307
AsuII TTCGAA 1 cut(s) 141
AsuNHI GCTAGC 1 cut(s) 905
AvaII GGWCC 1 cut(s) 870
BaeGI GKGCMC 1 cut(s) 1412
BalI TGGCCA 1 cut(s) 749
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 161
BauI CACGAG 1 cut(s) 318
BbrPI CACGTG 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 161
BbvCI CCTCAGC 3 cut(s) 720, 729, 1092
BbvI GCAGC 3 cut(s) 168, 582, 1181
BccI CCATC 5 cut(s) 206, 502, 658, 1292, 1399
BceAI ACGGC 1 cut(s) 173
BclI TGATCA 2 cut(s) 36, 1191
BcnI CCSGG 4 cut(s) 43, 93, 468, 763
BcoDI GTCTC 4 cut(s) 233, 308, 617, 1173
BfaI CTAG 4 cut(s) 273, 867, 906, 1431
BfmI CTRYAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 772
BglI GCCNNNNNGGC 4 cut(s) 767, 806, 974, 1016
BisI GCNGC 7 cut(s) 182, 330, 516, 596, 606, 801, 1170
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 7 cut(s) 183, 331, 517, 597, 607, 802, 1171
Bme1390I CCNGG 4 cut(s) 43, 93, 468, 763
Bme18I GGWCC 1 cut(s) 870
BmgBI CACGTC 3 cut(s) 118, 538, 1046
BmgT120I GGNCC 3 cut(s) 203, 870, 1105
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 4 cut(s) 43, 93, 468, 763
BmsI GCATC 2 cut(s) 814, 1138
BmtI GCTAGC 1 cut(s) 909
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 815
Bpu10I CCTNAGC 3 cut(s) 720, 729, 1092
Bpu1102I GCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 916, 1104
BpuMI CCSGG 4 cut(s) 43, 93, 468, 763
Bsa29I ATCGAT 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 50
BsaJI CCNNGG 6 cut(s) 91, 206, 342, 467, 1108, 1233
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 554, 584
Bsc4I CCNNNNNNNGG 2 cut(s) 867, 1144
Bse118I RCCGGY 1 cut(s) 1382
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 1323
Bse8I GATNNNNATC 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 6 cut(s) 91, 206, 342, 467, 1108, 1233
BseGI GGATG 7 cut(s) 219, 466, 669, 829, 858, 1153, 1410
BseJI GATNNNNATC 1 cut(s) 50
BseLI CCNNNNNNNGG 2 cut(s) 867, 1144
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 6 cut(s) 711, 720, 732, 879, 984, 1083
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 1412
BseXI GCAGC 3 cut(s) 168, 582, 1181
Bsh1236I CGCG 2 cut(s) 329, 1245
BshVI ATCGAT 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 7 cut(s) 42, 93, 467, 762, 1332, 1383, 1397
BslFI GGGAC 4 cut(s) 106, 376, 652, 1110
BslI CCNNNNNNNGG 2 cut(s) 867, 1144
BsmAI GTCTC 4 cut(s) 233, 308, 617, 1173
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 4 cut(s) 106, 376, 652, 1110
BsmI GAATGC 1 cut(s) 862
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 161, 1412
Bsp143I GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
Bsp1720I GCTNAGC 1 cut(s) 741
Bsp19I CCATGG 1 cut(s) 342
BspCNI CTCAG 6 cut(s) 712, 721, 733, 880, 983, 1084
BspDI ATCGAT 1 cut(s) 213
BspFNI CGCG 2 cut(s) 329, 1245
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 1 cut(s) 86
BspMAI CTGCAG 1 cut(s) 600
BspMI ACCTGC 1 cut(s) 772
BspOI GCTAGC 1 cut(s) 909
BspPI GGATC 5 cut(s) 53, 79, 92, 218, 308
BspT104I TTCGAA 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 1382
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 1382
BssECI CCNNGG 6 cut(s) 91, 206, 342, 467, 1108, 1233
BssMI GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 2 cut(s) 342, 1108
Bst2BI CACGAG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 1199, 1369
Bst6I CTCTTC 1 cut(s) 638
BstBAI YACGTR 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 4 cut(s) 69, 186, 747, 907
BstDEI CTNAG 7 cut(s) 720, 729, 741, 888, 970, 1092, 1221
BstDSI CCRYGG 2 cut(s) 206, 342
BstENI CCTNNNNNAGG 1 cut(s) 865
BstF5I GGATG 7 cut(s) 219, 466, 669, 829, 858, 1153, 1410
BstFNI CGCG 2 cut(s) 329, 1245
BstHHI GCGC 2 cut(s) 781, 1245
BstKTI GATC 7 cut(s) 39, 48, 87, 213, 303, 1194, 1227
BstMAI GTCTC 4 cut(s) 233, 308, 617, 1173
BstMBI GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
BstNSI RCATGY 2 cut(s) 1207, 1390
BstSCI CCNGG 4 cut(s) 41, 91, 466, 761
BstSFI CTRYAG 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 1412
BstUI CGCG 2 cut(s) 329, 1245
BstV1I GCAGC 3 cut(s) 168, 582, 1181
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Bsu15I ATCGAT 1 cut(s) 213
BsuTUI ATCGAT 1 cut(s) 213
BtgI CCRYGG 2 cut(s) 206, 342
BtrI CACGTC 3 cut(s) 118, 538, 1046
BtsCI GGATG 7 cut(s) 219, 466, 669, 829, 858, 1153, 1410
BtsIMutI CAGTG 1 cut(s) 1204
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 4 cut(s) 69, 186, 747, 907
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 2 cut(s) 781, 1245
Cfr10I RCCGGY 1 cut(s) 1382
Cfr13I GGNCC 3 cut(s) 203, 870, 1105
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 1 cut(s) 626
Csp6I GTAC 2 cut(s) 238, 929
CspCI CAANNNNNGTGG 2 cut(s) 792, 827
CviQI GTAC 2 cut(s) 238, 929
DdeI CTNAG 7 cut(s) 720, 729, 741, 888, 970, 1092, 1221
DpnI GATC 7 cut(s) 38, 47, 86, 212, 302, 1193, 1226
DpnII GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
EaeI YGGCCR 3 cut(s) 94, 606, 747
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
EciI GGCGGA 4 cut(s) 557, 683, 701, 803
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 342, 1108
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 870
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 2 cut(s) 6, 1235
Eco72I CACGTG 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 1034
EcoT14I CCWWGG 2 cut(s) 342, 1108
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 2 cut(s) 342, 1108
Esp3I CGTCTC 1 cut(s) 617
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FaqI GGGAC 4 cut(s) 106, 376, 652, 1110
FauI CCCGC 3 cut(s) 193, 263, 1274
FbaI TGATCA 2 cut(s) 36, 1191
Fnu4HI GCNGC 7 cut(s) 182, 330, 516, 596, 606, 801, 1170
FokI GGATG 7 cut(s) 206, 473, 676, 836, 865, 1160, 1417
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 7 cut(s) 182, 330, 516, 596, 606, 801, 1170
FspBI CTAG 4 cut(s) 273, 867, 906, 1431
GlaI GCGC 2 cut(s) 780, 1244
GluI GCNGC 7 cut(s) 182, 330, 516, 596, 606, 801, 1170
GsuI CTGGAG 1 cut(s) 815
HapII CCGG 7 cut(s) 42, 93, 467, 762, 1332, 1383, 1397
HgaI GACGC 1 cut(s) 626
HhaI GCGC 2 cut(s) 781, 1245
Hin6I GCGC 2 cut(s) 779, 1243
HinP1I GCGC 2 cut(s) 779, 1243
HincII GTYRAC 3 cut(s) 130, 535, 1049
HindII GTYRAC 3 cut(s) 130, 535, 1049
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 4 cut(s) 51, 226, 316, 1098
HpaII CCGG 7 cut(s) 42, 93, 467, 762, 1332, 1383, 1397
HphI GGTGA 6 cut(s) 31, 46, 46, 187, 691, 1307
Hpy166II GTNNAC 4 cut(s) 130, 535, 1049, 1339
Hpy188I TCNGA 5 cut(s) 25, 50, 163, 973, 1027
Hpy188III TCNNGA 5 cut(s) 223, 895, 1211, 1363, 1373
Hpy8I GTNNAC 4 cut(s) 130, 535, 1049, 1339
Hpy99I CGWCG 2 cut(s) 119, 1064
HpyAV CCTTC 9 cut(s) 70, 112, 148, 184, 388, 550, 676, 751, 1315
HpyCH4III ACNGT 2 cut(s) 1199, 1369
HpyCH4IV ACGT 4 cut(s) 117, 279, 537, 1045
HpyF3I CTNAG 7 cut(s) 720, 729, 741, 888, 970, 1092, 1221
HpySE526I ACGT 4 cut(s) 117, 279, 537, 1045
HspAI GCGC 2 cut(s) 779, 1243
Ksp22I TGATCA 2 cut(s) 36, 1191
Kzo9I GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
LmnI GCTCC 2 cut(s) 174, 790
Lsp1109I GCAGC 3 cut(s) 168, 582, 1181
LweI GCATC 2 cut(s) 814, 1138
MaeI CTAG 4 cut(s) 273, 867, 906, 1431
MaeII ACGT 4 cut(s) 117, 279, 537, 1045
MaeIII GTNAC 1 cut(s) 485
MalI GATC 7 cut(s) 38, 47, 86, 212, 302, 1193, 1226
MboI GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
MboII GAAGA 6 cut(s) 103, 184, 586, 640, 655, 766
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 2 cut(s) 161, 1412
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 5 cut(s) 978, 1034, 1067, 1215, 1420
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 3 cut(s) 235, 310, 1092
MmeI TCCRAC 3 cut(s) 384, 1005, 1135
Mox20I TGGCCA 1 cut(s) 749
MscI TGGCCA 1 cut(s) 749
MseI TTAA 3 cut(s) 357, 375, 675
Msp20I TGGCCA 1 cut(s) 749
MspI CCGG 7 cut(s) 42, 93, 467, 762, 1332, 1383, 1397
MspR9I CCNGG 4 cut(s) 43, 93, 468, 763
Mva1269I GAATGC 1 cut(s) 862
MvnI CGCG 2 cut(s) 329, 1245
NciI CCSGG 4 cut(s) 43, 93, 468, 763
NcoI CCATGG 1 cut(s) 342
NdeII GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
NheI GCTAGC 1 cut(s) 905
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 2 cut(s) 853, 1258
NspI RCATGY 2 cut(s) 1207, 1390
NspV TTCGAA 1 cut(s) 141
PagI TCATGA 1 cut(s) 1372
PaqCI CACCTGC 1 cut(s) 772
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 123
PctI GAATGC 1 cut(s) 862
PfeI GAWTC 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 1144
PkrI GCNGC 7 cut(s) 183, 331, 517, 597, 607, 802, 1171
PleI GAGTC 3 cut(s) 234, 310, 1092
PmaCI CACGTG 1 cut(s) 280
PmlI CACGTG 1 cut(s) 280
PpsI GAGTC 3 cut(s) 234, 310, 1092
Ppu21I YACGTR 1 cut(s) 280
PsiI TTATAA 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 161
PspCI CACGTG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 3 cut(s) 203, 870, 1105
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 2 cut(s) 239, 930
RsaNI GTAC 2 cut(s) 238, 929
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 3 cut(s) 357, 375, 675
SatI GCNGC 7 cut(s) 182, 330, 516, 596, 606, 801, 1170
Sau3AI GATC 7 cut(s) 36, 45, 84, 210, 300, 1191, 1224
Sau96I GGNCC 3 cut(s) 203, 870, 1105
SchI GAGTC 3 cut(s) 235, 310, 1092
ScrFI CCNGG 4 cut(s) 43, 93, 468, 763
SduI GDGCHC 2 cut(s) 161, 1412
SfaNI GCATC 2 cut(s) 814, 1138
SfcI CTRYAG 1 cut(s) 596
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 870
SmlI CTYRAG 2 cut(s) 895, 1119
SmoI CTYRAG 2 cut(s) 895, 1119
Sse9I AATT 5 cut(s) 978, 1034, 1067, 1215, 1420
SspMI CTAG 4 cut(s) 273, 867, 906, 1431
SstI GAGCTC 1 cut(s) 161
StyD4I CCNGG 4 cut(s) 41, 91, 466, 761
StyI CCWWGG 2 cut(s) 342, 1108
TaaI ACNGT 2 cut(s) 1199, 1369
TaiI ACGT 4 cut(s) 120, 282, 540, 1048
TaqI TCGA 5 cut(s) 114, 141, 213, 335, 572
TaqII GACCGA 1 cut(s) 887
TasI AATT 5 cut(s) 978, 1034, 1067, 1215, 1420
TauI GCSGC 4 cut(s) 332, 518, 608, 803
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 3 cut(s) 357, 375, 675
Tru9I TTAA 3 cut(s) 357, 375, 675
TscAI CASTG 1 cut(s) 1204
TseI GCWGC 3 cut(s) 181, 595, 1169
TspDTI ATGAA 7 cut(s) 94, 166, 869, 933, 1334, 1361, 1389
TspGWI ACGGA 3 cut(s) 223, 854, 1077
TspRI CASTG 1 cut(s) 1204
Van91I CCANNNNNTGG 1 cut(s) 1144
VpaK11BI GGWCC 1 cut(s) 870
XagI CCTNNNNNAGG 1 cut(s) 865
XapI RAATTY 3 cut(s) 1034, 1067, 1420
XceI RCATGY 2 cut(s) 1207, 1390
XspI CTAG 4 cut(s) 273, 867, 906, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.