Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0000793

Physical Location & Seq

Genomic Coordinates Reverse (-)

41708797 .. 41728017

Gene Structure 19221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0270200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGCCACTGATGATTCGAGTCATTTGACAATGGAGAAAGAAATTGTTCCAGCTTCTGAAAAAGAAGGAAAAGATGGGTTTCAGTTTAGGATTCTTGGTCGGCATCTTTGGAGTTCTAATTCTCTCTCATGCCGCTTAATCAACTGTTCAGTGCTGGTCTTTAATCCTATAATCAAGAAGCTGAGAAAACCAAGACTTTTGCTACTCCCAAAGACAGAGGTTTGGACTGGAAACAAAGAGGAAAGTTGGGGATTGAACATTACGTATATTGAGGGAGAGGCTGATAGTTATTATTCAAAACGTCAAGGGACGTACTTTTTTGATTTTATGCATAAAAGTTTTGGCACCATTTTTCTGCTGCTGTTCCTTTGTCTGCTACCCAATATAGTCTTGGTTTCATATTGTTATTCAGATTTTAGGAACAGTTTGCAGTCAGATTCTTTAACGGTTTTGGATCCAGGACTCGCTCGGCAGGTCTCGGCAGCTTCTCGGCAGGTCTCGGCAGGACTCGCTCGGCAGCTCTCGGCAGCTTCTCGGCAGATTTGGTCTTTATCTTCCAAGTTTGCAGTGATATCTTCCAGTGTGGTTGATTTGGGATCTGGGTCGGTGCCTGTTTTCGGATTTGGTGGTCTGTCAATGGGCGCATTGGATGAGGGGCATTTCTGTTCGGAACTTGAGAATGGAGTGAAGCTGGGTTGTATTGAGCTGAGTGTTGATGCCGAACCCGAGACCGTAGCTATTGAGGATGCTGTCAAAGTTCTCCTGCAGGGTCTTGGAGAGGATGTGAACAGAGAAGGTCTCAAGAAGACACCTCTTCGTGTTGCCAAGGCCCTTCGAGAAGGAACAAGGGGTTATAGACAAAAGGTTAAGGACATTGTGCAAGGTGCTTTGTTCCCTGAAGCTGGTCTGGATAATGCAGTAGGTCATGCTGGAGGAGCTGGTGGACTTGTGGTTGTTCGAGATCTGTACCTCTTCTCCTATTGTGAATCTTGTATGCTACCATTCCAGGTTAAATGTCATGTGGGTTATGTCCCTTCTGGTCAACGAGTTGTAGGCTTAAGCAAGCTCTCTCGAGTAGCTGATGTGTTTGGAAAACGTCTTCAAGAGCCGCAGCGTCTAGCAGATGAAGTGTGTTCAGCCCTGCACCATGGCATCAAGCCAGCCGGTGTCGCTGTCATGCTCCAGTGTTCACACATTCATTTCCCAAATTTAGAATCAGCTTTTCTCGACTCGAACCACCAAGGATGGGTAAAATTGCTGGTTAGTTCAGGTTCTGGAGTTTTTGAAAATGAAAGTGCCGATATTTGGGCAGATTTTTTGAGTCTTCTGAAATTCAGAGGCATAGATGTGGACAAAACTGGCTTGAGAGACTCAAGCAATTGGTGTCCATCTCGATCTTCAGCCGGTGCTAATGCTGTCTCCAAGGTTGAATCAGCCAATCAGGGAATGGTCACTGCAGTAGCTTCAATTCTTAGATCACTGGGTGAAGACCCATTGAGAAAGGAGCTCGTAGGAACACCTGTTCGCTTTGTGAAATGGTTGATGAACTTCCAGAATTGTAATTTGGATATGAAGCTGAATGGGTTTGTTTCTGCTAGGGTGGATCCTCTACACCCCAATGGAGATAGCTGCAAAGAAAAGCAAATCCACTCTGAGCTCAACTTGTCTGTGTGGTCTCAGTGTGAGCATCATTTACTTCCCTTTTATGGTGTCGTGCATATAGGATATACGGCCACAGAAGGATCCAATCCCATTGGAAAATCTCTTCTACAATCAATTGTCTGTTTCTATGGTTTTAAGCTCCAGGTGCAGGAGAGACTTACACGGCAGATAGCAGAAACTGTGGCATCACTTTCAGGTGGAGATGTGATAGTTGTTGTGGAGGCTAACCACACCTGTATGATCTCGAGAGGGATTGAGAAGTTTGGAAGTAGCACAGCTACCATCGCAGTACTGGGTCGTTTTTCAACTGACCCTGCTGCAAGGGCCAAGTTTTTGCAGAGCCTCCCAAACACTGCCATTTCAGGACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009877 GO:0016020 GO:0016324 GO:0022622 GO:0032501 GO:0032502 GO:0044403 GO:0044419 GO:0044425 GO:0044459 GO:0044464 GO:0045177 GO:0048364 GO:0048731 GO:0048856 GO:0051704 GO:0071944 GO:0098590 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Band_7

Protein Analysis

677

Amino Acids

73.73

Weight (kDa)

7.53

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_cyclohydroI	PF01227	247 - 399	2.7e-34	GTP cyclohydrolase I
GTP_cyclohydroI	PF01227	485 - 668	2.6e-37	GTP cyclohydrolase I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000793)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25250 AT5G25260 AT5G64870
fragaria_vesca	FvH4_2g28360 FvH4_2g28361 FvH4_2g28363 FvH4_6g10190
malus_domestica	MD08G1153100.v1.1 MD15G1129900.v1.1 MD15G1130000.v1.1 MD15G1130100.v1.1
prunus_persica	Prupe.1G485400_v2.0.a1 Prupe.1G485500_v2.0.a1 Prupe.1G485600_v2.0.a1 Prupe.1G485600_v2.0.a1
pyrus_communis	pycom08g13240
rosa_chinensis	RchiOBHm_Chr3g0460921 RchiOBHm_Chr4g0425621 RchiOBHm_Chr4g0425631 RchiOBHm_Chr6g0297281 RchiOBHm_Chr6g0297301 RchiOBHm_Chr6g0297311
rosa_laevigata	RLG00000007352 RLG00000011612 RLG00000011613 RLG00000011615 RLG00000024965
rosa_multiflora	Rmu_co8465209.1_g000001 Rmu_sc0003958.1_g000017 Rmu_sc0004535.1_g000003 Rmu_sc0004535.1_g000007 Rmu_sc0004535.1_g000008 Rmu_sc0013249.1_g000009 Rmu_sc0021377.1_g000005
rosa_roxburghii	Rroxscaffold_4G00306830 Rroxscaffold_6G00419030 Rroxscaffold_7G00170670 Rroxscaffold_7G00170690
rosa_rugosa	Rorug03G0047100 Rorug04G0204200 Rorug04G0204400 Rorug06G0270200 Rorug06G0270500 Rorug06G0270600
rosa_samantha	Rh3AG104500 Rh3BG108200 Rh3CG109900 Rh3DG110000 Rh4AG261700 Rh4BG267600 Rh4CG278500 Rh4CG278700 Rh4DG263500 Rh6AG381700 Rh6AG381900 Rh6AG382000 Rh6BG389700 Rh6BG389900 Rh6BG390000 Rh6CG394900 Rh6CG395100 Rh6CG395200 Rh6DG381600 Rh6DG381900 Rh6DG382000
rosa_wichuraiana	Rw3G008900 Rw4G022620 Rw6G033250 Rw6G033270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	463, 484
AccB1I	GGYRCC	2 cut(s)	344, 607
AciI	CCGC	2 cut(s)	133, 1109
AclWI	GGATC	7 cut(s)	449, 462, 604, 1598, 1611, 1737, 1750
AcoI	YGGCCR	2 cut(s)	3, 1731
AcsI	RAATTY	2 cut(s)	1207, 1331
AcuI	CTGAAG	2 cut(s)	918, 1383
AdeI	CACNNNGTG	1 cut(s)	1484
AfaI	GTAC	3 cut(s)	314, 968, 1953
AfiI	CCNNNNNNNGG	6 cut(s)	617, 902, 1246, 1305, 1706, 1810
AflII	CTTAAG	1 cut(s)	1057
AgsI	TTSAA	7 cut(s)	256, 297, 1103, 1286, 1430, 1467, 1968
AjnI	CCWGG	3 cut(s)	457, 1005, 1803
AjuI	GAANNNNNNNTTGG	2 cut(s)	1547, 1579
AloI	GAACNNNNNNTCC	2 cut(s)	1506, 1538
Alw21I	GWGCWC	2 cut(s)	1509, 1659
Alw26I	GTCTC	8 cut(s)	481, 502, 722, 803, 1362, 1423, 1680, 1810
AlwI	GGATC	7 cut(s)	449, 462, 604, 1598, 1611, 1737, 1750
AlwNI	CAGNNNCTG	3 cut(s)	56, 1274, 1841
Ama87I	CYCGRG	3 cut(s)	725, 1071, 1906
AoxI	GGCC	4 cut(s)	3, 828, 1731, 1986
ApeKI	GCWGC	7 cut(s)	358, 482, 517, 527, 1111, 1629, 1979
ApoI	RAATTY	2 cut(s)	1207, 1331
Asp700I	GAANNNNTTC	1 cut(s)	45
AspLEI	GCGC	1 cut(s)	644
AspS9I	GGNCC	3 cut(s)	829, 1986, 2027
AsuHPI	GGTGA	1 cut(s)	1496
AvaI	CYCGRG	3 cut(s)	725, 1071, 1906
AvaII	GGWCC	1 cut(s)	2027
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	3 cut(s)	454, 1603, 1742
BanI	GGYRCC	2 cut(s)	344, 607
BanII	GRGCYC	2 cut(s)	1509, 1659
BbsI	GAAGAC	4 cut(s)	812, 1091, 1316, 1494
Bbv12I	GWGCWC	2 cut(s)	1509, 1659
BbvI	GCAGC	7 cut(s)	345, 494, 529, 539, 1123, 1616, 1966
BccI	CCATC	4 cut(s)	68, 1239, 1396, 1952
BceAI	ACGGC	2 cut(s)	1746, 1841
BciT130I	CCWGG	3 cut(s)	459, 1007, 1805
BcoDI	GTCTC	8 cut(s)	481, 502, 722, 803, 1362, 1423, 1680, 1810
BfaI	CTAG	2 cut(s)	1118, 1596
BfmI	CTRYAG	2 cut(s)	764, 1455
BfrI	CTTAAG	1 cut(s)	1057
BfuAI	ACCTGC	2 cut(s)	463, 484
BglII	AGATCT	1 cut(s)	961
BisI	GCNGC	9 cut(s)	133, 359, 483, 518, 528, 1109, 1112, 1630, 1980
BlsI	GCNGC	9 cut(s)	134, 360, 484, 519, 529, 1110, 1113, 1631, 1981
BmcAI	AGTACT	1 cut(s)	1953
Bme1390I	CCNGG	3 cut(s)	459, 1007, 1805
Bme18I	GGWCC	1 cut(s)	2027
BmeT110I	CYCGRG	3 cut(s)	725, 1071, 1906
BmgT120I	GGNCC	3 cut(s)	829, 1986, 2027
BmiI	GGNNCC	5 cut(s)	346, 456, 609, 1605, 1744
BmrFI	CCNGG	3 cut(s)	459, 1007, 1805
BmrI	ACTGGG	2 cut(s)	1490, 1964
BmsI	GCATC	6 cut(s)	112, 706, 736, 1161, 1696, 1856
BmuI	ACTGGG	2 cut(s)	1490, 1964
BpiI	GAAGAC	4 cut(s)	812, 1091, 1316, 1494
BplI	GAGNNNNNCTC	2 cut(s)	783, 815
BpmI	CTGGAG	4 cut(s)	951, 1166, 1296, 1787
BpuEI	CTTGAG	4 cut(s)	695, 785, 1357, 1384
BsaAI	YACGTR	1 cut(s)	264
BsaI	GGTCTC	5 cut(s)	481, 502, 722, 803, 1680
BsaJI	CCNNGG	4 cut(s)	825, 1147, 1240, 1422
BsaXI	ACNNNNNCTCC	4 cut(s)	768, 798, 959, 989
Bsc4I	CCNNNNNNNGG	6 cut(s)	617, 902, 1246, 1305, 1706, 1810
Bse118I	RCCGGY	2 cut(s)	1163, 1403
Bse1I	ACTGG	6 cut(s)	232, 579, 1183, 1363, 1485, 1959
BseBI	CCWGG	3 cut(s)	459, 1007, 1805
BseDI	CCNNGG	4 cut(s)	825, 1147, 1240, 1422
BseGI	GGATG	4 cut(s)	655, 751, 787, 1250
BseLI	CCNNNNNNNGG	6 cut(s)	617, 902, 1246, 1305, 1706, 1810
BseMII	CTCAG	4 cut(s)	173, 698, 1644, 1691
BseNI	ACTGG	6 cut(s)	232, 579, 1183, 1363, 1485, 1959
BseRI	GAGGAG	1 cut(s)	948
BseXI	GCAGC	7 cut(s)	345, 494, 529, 539, 1123, 1616, 1966
BseYI	CCCAGC	1 cut(s)	691
BsgI	GTGCAG	2 cut(s)	1127, 1829
BshFI	GGCC	4 cut(s)	5, 830, 1733, 1988
BshNI	GGYRCC	2 cut(s)	344, 607
BsiHKAI	GWGCWC	2 cut(s)	1509, 1659
BsiHKCI	CYCGRG	3 cut(s)	725, 1071, 1906
BsiSI	CCGG	2 cut(s)	1164, 1404
BslFI	GGGAC	2 cut(s)	322, 1016
BslI	CCNNNNNNNGG	6 cut(s)	617, 902, 1246, 1305, 1706, 1810
BsmAI	GTCTC	8 cut(s)	481, 502, 722, 803, 1362, 1423, 1680, 1810
BsmFI	GGGAC	2 cut(s)	322, 1016
BsnI	GGCC	4 cut(s)	5, 830, 1733, 1988
Bso31I	GGTCTC	5 cut(s)	481, 502, 722, 803, 1680
BsoBI	CYCGRG	3 cut(s)	725, 1071, 1906
Bsp1286I	GDGCHC	2 cut(s)	1509, 1659
Bsp143I	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
Bsp19I	CCATGG	1 cut(s)	1147
BspACI	CCGC	2 cut(s)	133, 1109
BspANI	GGCC	4 cut(s)	5, 830, 1733, 1988
BspCNI	CTCAG	4 cut(s)	174, 699, 1645, 1690
BspLI	GGNNCC	5 cut(s)	346, 456, 609, 1605, 1744
BspMAI	CTGCAG	2 cut(s)	768, 1459
BspMI	ACCTGC	2 cut(s)	463, 484
BspPI	GGATC	7 cut(s)	449, 462, 604, 1598, 1611, 1737, 1750
BspT107I	GGYRCC	2 cut(s)	344, 607
BspTI	CTTAAG	1 cut(s)	1057
BspTNI	GGTCTC	5 cut(s)	481, 502, 722, 803, 1680
BsrFI	RCCGGY	2 cut(s)	1163, 1403
BsrI	ACTGG	6 cut(s)	232, 579, 1183, 1363, 1485, 1959
BssAI	RCCGGY	2 cut(s)	1163, 1403
BssECI	CCNNGG	4 cut(s)	825, 1147, 1240, 1422
BssMI	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
BssT1I	CCWWGG	4 cut(s)	825, 1147, 1240, 1422
Bst2UI	CCWGG	3 cut(s)	459, 1007, 1805
Bst4CI	ACNGT	6 cut(s)	146, 425, 448, 733, 1843, 2031
Bst6I	CTCTTC	3 cut(s)	819, 977, 1770
BstAFI	CTTAAG	1 cut(s)	1057
BstBAI	YACGTR	1 cut(s)	264
BstC8I	GCNNGC	2 cut(s)	1064, 1161
BstDEI	CTNAG	5 cut(s)	182, 707, 1472, 1653, 1677
BstDSI	CCRYGG	1 cut(s)	1147
BstF5I	GGATG	4 cut(s)	655, 751, 787, 1250
BstHHI	GCGC	1 cut(s)	644
BstKTI	GATC	8 cut(s)	457, 599, 964, 1397, 1478, 1606, 1745, 1905
BstMAI	GTCTC	8 cut(s)	481, 502, 722, 803, 1362, 1423, 1680, 1810
BstMBI	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
BstMWI	GCNNNNNNNGC	6 cut(s)	509, 935, 1169, 1807, 1946, 1985
BstNI	CCWGG	3 cut(s)	459, 1007, 1805
BstSCI	CCNGG	3 cut(s)	457, 1005, 1803
BstSFI	CTRYAG	2 cut(s)	764, 1455
BstSNI	TACGTA	1 cut(s)	264
BstV1I	GCAGC	7 cut(s)	345, 494, 529, 539, 1123, 1616, 1966
BstV2I	GAAGAC	4 cut(s)	812, 1091, 1316, 1494
BstX2I	RGATCY	5 cut(s)	454, 596, 961, 1603, 1742
BstYI	RGATCY	5 cut(s)	454, 596, 961, 1603, 1742
BsuRI	GGCC	4 cut(s)	5, 830, 1733, 1988
BtgI	CCRYGG	1 cut(s)	1147
BtgZI	GCGATG	1 cut(s)	1930
BtsCI	GGATG	4 cut(s)	655, 751, 787, 1250
BtsI	GCAGTG	3 cut(s)	573, 1452, 2013
BtsIMutI	CAGTG	9 cut(s)	6, 156, 573, 586, 1190, 1452, 1478, 1685, 2013
BveI	ACCTGC	2 cut(s)	463, 484
Cac8I	GCNNGC	2 cut(s)	1064, 1161
CaiI	CAGNNNCTG	3 cut(s)	56, 1274, 1841
CfoI	GCGC	1 cut(s)	644
Cfr10I	RCCGGY	2 cut(s)	1163, 1403
Cfr13I	GGNCC	3 cut(s)	829, 1986, 2027
CseI	GACGC	1 cut(s)	1103
Csp6I	GTAC	3 cut(s)	313, 967, 1952
CviAII	CATG	5 cut(s)	129, 926, 1019, 1148, 1177
CviQI	GTAC	3 cut(s)	313, 967, 1952
DdeI	CTNAG	5 cut(s)	182, 707, 1472, 1653, 1677
DpnI	GATC	8 cut(s)	456, 598, 963, 1396, 1477, 1605, 1744, 1904
DpnII	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
DraIII	CACNNNGTG	1 cut(s)	1484
EaeI	YGGCCR	2 cut(s)	3, 1731
Eam1104I	CTCTTC	3 cut(s)	819, 977, 1770
EarI	CTCTTC	3 cut(s)	819, 977, 1770
Ecl136II	GAGCTC	2 cut(s)	1507, 1657
Eco105I	TACGTA	1 cut(s)	264
Eco130I	CCWWGG	4 cut(s)	825, 1147, 1240, 1422
Eco24I	GRGCYC	2 cut(s)	1509, 1659
Eco31I	GGTCTC	5 cut(s)	481, 502, 722, 803, 1680
Eco32I	GATATC	1 cut(s)	573
Eco47I	GGWCC	1 cut(s)	2027
Eco53kI	GAGCTC	2 cut(s)	1507, 1657
Eco57I	CTGAAG	2 cut(s)	918, 1383
Eco88I	CYCGRG	3 cut(s)	725, 1071, 1906
EcoICRI	GAGCTC	2 cut(s)	1507, 1657
EcoO109I	RGGNCCY	1 cut(s)	829
EcoRII	CCWGG	3 cut(s)	457, 1005, 1803
EcoRV	GATATC	1 cut(s)	573
EcoT14I	CCWWGG	4 cut(s)	825, 1147, 1240, 1422
EcoT22I	ATGCAT	1 cut(s)	333
EcoT38I	GRGCYC	2 cut(s)	1509, 1659
ErhI	CCWWGG	4 cut(s)	825, 1147, 1240, 1422
FaeI	CATG	5 cut(s)	132, 929, 1022, 1151, 1180
FaqI	GGGAC	2 cut(s)	322, 1016
FatI	CATG	5 cut(s)	128, 925, 1018, 1147, 1176
Fnu4HI	GCNGC	9 cut(s)	133, 359, 483, 518, 528, 1109, 1112, 1630, 1980
FokI	GGATG	4 cut(s)	662, 758, 794, 1257
FriOI	GRGCYC	2 cut(s)	1509, 1659
Fsp4HI	GCNGC	9 cut(s)	133, 359, 483, 518, 528, 1109, 1112, 1630, 1980
FspBI	CTAG	2 cut(s)	1118, 1596
GlaI	GCGC	1 cut(s)	643
GluI	GCNGC	9 cut(s)	133, 359, 483, 518, 528, 1109, 1112, 1630, 1980
GsaI	CCCAGC	1 cut(s)	695
GsuI	CTGGAG	4 cut(s)	951, 1166, 1296, 1787
HaeIII	GGCC	4 cut(s)	5, 830, 1733, 1988
HapII	CCGG	2 cut(s)	1164, 1404
HgaI	GACGC	1 cut(s)	1103
HhaI	GCGC	1 cut(s)	644
Hin1II	CATG	5 cut(s)	132, 929, 1022, 1151, 1180
Hin6I	GCGC	1 cut(s)	642
HinP1I	GCGC	1 cut(s)	642
HincII	GTYRAC	1 cut(s)	1043
HindII	GTYRAC	1 cut(s)	1043
HpaII	CCGG	2 cut(s)	1164, 1404
HphI	GGTGA	1 cut(s)	1496
Hpy166II	GTNNAC	5 cut(s)	787, 944, 1043, 1190, 1351
Hpy188I	TCNGA	8 cut(s)	58, 412, 436, 620, 670, 1329, 1337, 1654
Hpy8I	GTNNAC	5 cut(s)	787, 944, 1043, 1190, 1351
HpyAV	CCTTC	6 cut(s)	59, 788, 833, 842, 1044, 1733
HpyCH4III	ACNGT	6 cut(s)	146, 425, 448, 733, 1843, 2031
HpyCH4IV	ACGT	4 cut(s)	263, 301, 311, 1096
HpyF10VI	GCNNNNNNNGC	6 cut(s)	509, 935, 1169, 1807, 1946, 1985
HpyF3I	CTNAG	5 cut(s)	182, 707, 1472, 1653, 1677
HpySE526I	ACGT	4 cut(s)	263, 301, 311, 1096
Hsp92II	CATG	5 cut(s)	132, 929, 1022, 1151, 1180
HspAI	GCGC	1 cut(s)	642
Kzo9I	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
LmnI	GCTCC	4 cut(s)	935, 1185, 1504, 1806
Lsp1109I	GCAGC	7 cut(s)	345, 494, 529, 539, 1123, 1616, 1966
LweI	GCATC	6 cut(s)	112, 706, 736, 1161, 1696, 1856
MaeI	CTAG	2 cut(s)	1118, 1596
MaeII	ACGT	4 cut(s)	263, 301, 311, 1096
MaeIII	GTNAC	1 cut(s)	1450
MalI	GATC	8 cut(s)	456, 598, 963, 1396, 1477, 1605, 1744, 1904
MboI	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
MfeI	CAATTG	2 cut(s)	1378, 1776
MflI	RGATCY	5 cut(s)	454, 596, 961, 1603, 1742
MhlI	GDGCHC	2 cut(s)	1509, 1659
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	6 cut(s)	28, 456, 501, 1223, 1330, 1364
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	333
MroXI	GAANNNNTTC	1 cut(s)	45
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	137, 162, 443, 867, 1011, 1058, 1797
MslI	CAYNNNNRTG	3 cut(s)	1346, 1617, 1898
Msp20I	TGGCCA	1 cut(s)	5
MspCI	CTTAAG	1 cut(s)	1057
MspI	CCGG	2 cut(s)	1164, 1404
MspR9I	CCNGG	3 cut(s)	459, 1007, 1805
MunI	CAATTG	2 cut(s)	1378, 1776
MvaI	CCWGG	3 cut(s)	459, 1007, 1805
MwoI	GCNNNNNNNGC	6 cut(s)	509, 935, 1169, 1807, 1946, 1985
NcoI	CCATGG	1 cut(s)	1147
NdeII	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
NlaIII	CATG	5 cut(s)	132, 929, 1022, 1151, 1180
NlaIV	GGNNCC	5 cut(s)	346, 456, 609, 1605, 1744
NmeAIII	GCCGAG	7 cut(s)	448, 458, 469, 479, 493, 503, 514
NmuCI	GTSAC	1 cut(s)	1450
NsiI	ATGCAT	1 cut(s)	333
PaeR7I	CTCGAG	2 cut(s)	1071, 1906
PdmI	GAANNNNTTC	1 cut(s)	45
PfeI	GAWTC	6 cut(s)	14, 91, 437, 986, 1214, 1430
PfoI	TCCNGGA	1 cut(s)	457
PkrI	GCNGC	9 cut(s)	134, 360, 484, 519, 529, 1110, 1113, 1631, 1981
PleI	GAGTC	6 cut(s)	27, 456, 501, 1223, 1329, 1364
PpsI	GAGTC	6 cut(s)	27, 456, 501, 1223, 1329, 1364
Ppu21I	YACGTR	1 cut(s)	264
Psp124BI	GAGCTC	2 cut(s)	1509, 1659
Psp6I	CCWGG	3 cut(s)	457, 1005, 1803
PspFI	CCCAGC	1 cut(s)	691
PspGI	CCWGG	3 cut(s)	457, 1005, 1803
PspN4I	GGNNCC	5 cut(s)	346, 456, 609, 1605, 1744
PspPI	GGNCC	3 cut(s)	829, 1986, 2027
PstI	CTGCAG	2 cut(s)	768, 1459
PstNI	CAGNNNCTG	3 cut(s)	56, 1274, 1841
PsuI	RGATCY	5 cut(s)	454, 596, 961, 1603, 1742
RsaI	GTAC	3 cut(s)	314, 968, 1953
RsaNI	GTAC	3 cut(s)	313, 967, 1952
RseI	CAYNNNNRTG	3 cut(s)	1346, 1617, 1898
SacI	GAGCTC	2 cut(s)	1509, 1659
SaqAI	TTAA	7 cut(s)	137, 162, 443, 867, 1011, 1058, 1797
SatI	GCNGC	9 cut(s)	133, 359, 483, 518, 528, 1109, 1112, 1630, 1980
Sau3AI	GATC	8 cut(s)	454, 596, 961, 1394, 1475, 1603, 1742, 1902
Sau96I	GGNCC	3 cut(s)	829, 1986, 2027
SbfI	CCTGCAGG	1 cut(s)	768
ScaI	AGTACT	1 cut(s)	1953
SchI	GAGTC	6 cut(s)	28, 456, 501, 1223, 1330, 1364
ScrFI	CCNGG	3 cut(s)	459, 1007, 1805
SdaI	CCTGCAGG	1 cut(s)	768
SduI	GDGCHC	2 cut(s)	1509, 1659
SfaNI	GCATC	6 cut(s)	112, 706, 736, 1161, 1696, 1856
SfcI	CTRYAG	2 cut(s)	764, 1455
Sfr274I	CTCGAG	2 cut(s)	1071, 1906
SinI	GGWCC	1 cut(s)	2027
SlaI	CTCGAG	2 cut(s)	1071, 1906
SmiMI	CAYNNNNRTG	3 cut(s)	1346, 1617, 1898
SmlI	CTYRAG	7 cut(s)	674, 800, 1057, 1071, 1363, 1372, 1906
SmoI	CTYRAG	7 cut(s)	674, 800, 1057, 1071, 1363, 1372, 1906
SnaBI	TACGTA	1 cut(s)	264
Sse8387I	CCTGCAGG	1 cut(s)	768
SsiI	CCGC	2 cut(s)	133, 1109
SspMI	CTAG	2 cut(s)	1118, 1596
SstI	GAGCTC	2 cut(s)	1509, 1659
StyD4I	CCNGG	3 cut(s)	457, 1005, 1803
StyI	CCWWGG	4 cut(s)	825, 1147, 1240, 1422
TaaI	ACNGT	6 cut(s)	146, 425, 448, 733, 1843, 2031
TaiI	ACGT	4 cut(s)	266, 304, 314, 1099
TaqI	TCGA	8 cut(s)	17, 835, 958, 1072, 1227, 1232, 1393, 1907
TatI	WGTACW	1 cut(s)	1951
TauI	GCSGC	2 cut(s)	135, 1111
TfiI	GAWTC	6 cut(s)	14, 91, 437, 986, 1214, 1430
Tru1I	TTAA	7 cut(s)	137, 162, 443, 867, 1011, 1058, 1797
Tru9I	TTAA	7 cut(s)	137, 162, 443, 867, 1011, 1058, 1797
TscAI	CASTG	9 cut(s)	13, 156, 573, 586, 1190, 1459, 1485, 1685, 2020
TseFI	GTSAC	1 cut(s)	1450
TseI	GCWGC	7 cut(s)	358, 482, 517, 527, 1111, 1629, 1979
Tsp45I	GTSAC	1 cut(s)	1450
TspDTI	ATGAA	6 cut(s)	387, 1140, 1187, 1305, 1559, 1586
TspRI	CASTG	9 cut(s)	13, 156, 573, 586, 1190, 1459, 1485, 1685, 2020
Vha464I	CTTAAG	1 cut(s)	1057
VpaK11BI	GGWCC	1 cut(s)	2027
XapI	RAATTY	2 cut(s)	1207, 1331
XcmI	CCANNNNNNNNNTGG	3 cut(s)	388, 1444, 1951
XhoI	CTCGAG	2 cut(s)	1071, 1906
XmnI	GAANNNNTTC	1 cut(s)	45
XspI	CTAG	2 cut(s)	1118, 1596
ZrmI	AGTACT	1 cut(s)	1953
Zsp2I	ATGCAT	1 cut(s)	333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.