pycom08g21090

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004699
Physical Location & Seq
Forward (+)
20681168 .. 20683031
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGGCAACGACGGCGTGTTTCATCATCGTCAGCAGAAACGACATCCCTATTTACGAAGCTGAAGTCGGATCCGCGGCCAAAAGAGAAGATGCTGCACAGTTGCATCAGTTCATATTACATGCGGCTCTCGACATTGTTCAGGACCTTGCCTGGACTACCAGTGCCATGTACTTGAAAGCAATCGATCGCTTTAATGATTTGGTGGTTTCCGTCTATGTTACAGCTGACATGCACGACTTATGCTACTTCATGACTCTCGTAACGATGATGGCATCAAGAGCTTCTTCCAAGAGGTTCATGAGCTTTACATCAAAACTCTTCTCAATCCCCTCTACCTGCCTGGTTCACGAATTGCGTCATCACATTTTGATACAAAAGTTCGTGCTCTTGCACGAAAATATCTGTAGGGGCAGTGTATGCTGTGGGTACGCAAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sedlin_N

Protein Analysis

147

Amino Acids

16.4

Weight (kDa)

6.63

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sedlin_N PF04628 9 - 81 3.3e-20 Sedlin, N-terminal conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 344
AccII CGCG 1 cut(s) 74
AciI CCGC 3 cut(s) 72, 74, 122
AclWI GGATC 2 cut(s) 63, 76
AcoI YGGCCR 1 cut(s) 75
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 170, 428
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 149, 339
AluBI AGCT 5 cut(s) 59, 224, 281, 303, 435
AluI AGCT 5 cut(s) 59, 224, 281, 303, 435
Alw21I GWGCWC 1 cut(s) 387
AlwI GGATC 2 cut(s) 63, 76
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
AspS9I GGNCC 1 cut(s) 142
AvaII GGWCC 1 cut(s) 142
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BamHI GGATCC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 79
BccI CCATC 1 cut(s) 262
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 151, 341
BfmI CTRYAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 3 cut(s) 75, 93, 123
BlsI GCNGC 3 cut(s) 76, 94, 124
Bme1390I CCNGG 2 cut(s) 151, 341
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 2 cut(s) 151, 341
BmsI GCATC 3 cut(s) 79, 112, 281
Bsa29I ATCGAT 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 159
BseBI CCWGG 2 cut(s) 151, 341
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 387
BsnI GGCC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 68, 184
BspACI CCGC 3 cut(s) 72, 74, 122
BspANI GGCC 1 cut(s) 77
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 2 cut(s) 249, 297
BspLI GGNNCC 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 2 cut(s) 63, 76
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 68, 184
Bst2UI CCWGG 2 cut(s) 151, 341
Bst4CI ACNGT 1 cut(s) 99
Bst6I CTCTTC 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 433
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 42
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 71, 187
BstMBI GATC 2 cut(s) 68, 184
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 11, 278, 417
BstNI CCWGG 2 cut(s) 151, 341
BstNSI RCATGY 2 cut(s) 122, 232
BstSCI CCNGG 2 cut(s) 149, 339
BstSFI CTRYAG 1 cut(s) 403
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 1 cut(s) 77
BsuTUI ATCGAT 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 42
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 166, 418
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 433
CciI TCATGA 2 cut(s) 249, 297
Cfr13I GGNCC 1 cut(s) 142
Cfr42I CCGCGG 1 cut(s) 75
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 169, 427
CviAII CATG 5 cut(s) 119, 166, 229, 250, 298
CviJI RGCY 7 cut(s) 59, 77, 125, 224, 281, 303, 435
CviKI_1 RGCY 7 cut(s) 59, 77, 125, 224, 281, 303, 435
CviQI GTAC 2 cut(s) 169, 427
DpnI GATC 2 cut(s) 70, 186
DpnII GATC 2 cut(s) 68, 184
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 149, 339
FaeI CATG 5 cut(s) 122, 169, 232, 253, 301
FaiI YATR 9 cut(s) 113, 120, 167, 216, 230, 241, 251, 299, 418
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FatI CATG 5 cut(s) 118, 165, 228, 249, 297
Fnu4HI GCNGC 3 cut(s) 75, 93, 123
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 3 cut(s) 75, 93, 123
GluI GCNGC 3 cut(s) 75, 93, 123
HaeIII GGCC 1 cut(s) 77
HgaI GACGC 1 cut(s) 344
Hin1II CATG 5 cut(s) 122, 169, 232, 253, 301
HinfI GANTC 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 7 cut(s) 128, 140, 250, 276, 298, 347, 438
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 13
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4V TGCA 4 cut(s) 95, 103, 232, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 278, 417
Hsp92II CATG 5 cut(s) 122, 169, 232, 253, 301
KspI CCGCGG 1 cut(s) 75
Kzo9I GATC 2 cut(s) 68, 184
LpnPI CCDG 7 cut(s) 125, 136, 163, 172, 326, 349, 353
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 3 cut(s) 79, 112, 281
MaeIII GTNAC 2 cut(s) 217, 259
MalI GATC 2 cut(s) 70, 186
MboI GATC 2 cut(s) 68, 184
MboII GAAGA 3 cut(s) 98, 276, 310
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 350
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 2 cut(s) 285, 340
MseI TTAA 1 cut(s) 192
MspA1I CMGCKG 2 cut(s) 74, 224
MspR9I CCNGG 2 cut(s) 151, 341
MvaI CCWGG 2 cut(s) 151, 341
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 11, 278, 417
NdeII GATC 2 cut(s) 68, 184
NlaIII CATG 5 cut(s) 122, 169, 232, 253, 301
NlaIV GGNNCC 1 cut(s) 70
NspI RCATGY 2 cut(s) 122, 232
PagI TCATGA 2 cut(s) 249, 297
PkrI GCNGC 3 cut(s) 76, 94, 124
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 2 cut(s) 149, 339
PspGI CCWGG 2 cut(s) 149, 339
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 142
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 1 cut(s) 68
PvuI CGATCG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 2 cut(s) 170, 428
RsaNI GTAC 2 cut(s) 169, 427
SacII CCGCGG 1 cut(s) 75
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 75, 93, 123
Sau3AI GATC 2 cut(s) 68, 184
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 151, 341
SduI GDGCHC 1 cut(s) 387
SetI ASST 8 cut(s) 61, 147, 226, 283, 296, 305, 338, 437
SfaNI GCATC 3 cut(s) 79, 112, 281
SfcI CTRYAG 1 cut(s) 403
Sfr303I CCGCGG 1 cut(s) 75
SgrBI CCGCGG 1 cut(s) 75
SinI GGWCC 1 cut(s) 142
Sse9I AATT 1 cut(s) 350
SsiI CCGC 3 cut(s) 72, 74, 122
StyD4I CCNGG 2 cut(s) 149, 339
TaaI ACNGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 129, 183
TasI AATT 1 cut(s) 350
TatI WGTACW 1 cut(s) 168
TauI GCSGC 2 cut(s) 77, 125
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 166, 418
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 4 cut(s) 10, 100, 238, 286
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 2 cut(s) 166, 418
VpaK11BI GGWCC 1 cut(s) 142
XceI RCATGY 2 cut(s) 122, 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.