pycom09g00360

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013702
Physical Location & Seq
Forward (+)
304769 .. 306362
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
ATGAAATCAGTGTCACTTATTTTCTCGTTCATAGTTATGTTCTTGCATTTTCACTCATTGCAGATGCATAGTTTGCCAATTGCACCAGCGCTGTATGTGTTTGGGGATTCACTTTTTGACAGTGGTAACAATAACTTTTTACCAACTATTTGTAAGGCAGACTACTTGCCTTATGGGGTGAATTTTGTTAAGGGAGTCACTGGAAGATTTACCAACGGAAGAACAGTAGCAGATTTTACAGATGAGTTTCTTGGCCTACCATATCCTCCCCCATACATGAGCATACATAGATCGGCGACAGCGCTTACAGGCCTGAATTATGCATCTGGCTCTTGTGGCATTCTGCCAGAAACTGGAAGCCAATATGGAAAATGTTTGAATCTAAAGGACCAGATGGATTTGTTTCGAAGAACCGTGAAGTCGGACTTGCCAGGACAAATAAAAAATCAAACTGATCTTCTGCAGTACTTGTCCAAATCTATATTTCTGTTTTCTGTTGGCAGCAATGATTTCATCAACAACTACCTCGAACCTGAAGTTTTCGATACAAGCAAACGCTACTCTCCTCAACAATTTGCACAGATCTTAATGGTTACAATTGCTCATCATATGGAGAGGTTATACAACCTAGGAGCCAGGAAAGTAGTCATGTTTGAAATTGGTCCACTTGGATGCATTCCATCCATTGCAAAAACACACAAACACAGTGGAAACTGTGTGGAAAGTACAAATGAGCTAGCCTCGATTTTCAATGACAAGCTTCGTGCAACACTTGCGAATTTAACAACCACTTTCCAAGGTTCTTTCTTTGTTCTCGGTCGAGCAAACTGGATTGGCTACGATGCAATTACAAGTCCTGTTAAATATGGGCTAATGGATGGAAGCAATCCATGCTGTACTACTTGGGAAAATGGGACTTCAGGGTGCATTCCATTGCTTAGACCGTGCTTCGAACCAAATAACCACTTCTTTTGGGATGCTTATCATCTAACGGAATATGCAGGCTCAGTTATAGCAACAGGTTGCTTCAATGGCTCAACCGTTTGCACCCCTTTAAACATTAAGCAACTTGTGAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

361

Amino Acids

40.0

Weight (kDa)

7.55

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 339 4.5e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AcsI RAATTY 2 cut(s) 181, 778
AcuI CTGAAG 2 cut(s) 555, 903
AfaI GTAC 3 cut(s) 467, 727, 898
AfeI AGCGCT 2 cut(s) 90, 303
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 4 cut(s) 379, 656, 751, 1030
AjnI CCWGG 2 cut(s) 430, 635
AluBI AGCT 2 cut(s) 736, 760
AluI AGCT 2 cut(s) 736, 760
AlwNI CAGNNNCTG 1 cut(s) 353
Aor51HI AGCGCT 2 cut(s) 90, 303
AoxI GGCC 2 cut(s) 253, 310
ApeKI GCWGC 1 cut(s) 501
ApoI RAATTY 2 cut(s) 181, 778
AspA2I CCTAGG 1 cut(s) 628
AspLEI GCGC 2 cut(s) 91, 304
AspS9I GGNCC 2 cut(s) 388, 662
AsuHPI GGTGA 1 cut(s) 190
AsuII TTCGAA 2 cut(s) 406, 951
AsuNHI GCTAGC 1 cut(s) 736
AvaII GGWCC 2 cut(s) 388, 662
AvrII CCTAGG 1 cut(s) 628
BbvI GCAGC 1 cut(s) 513
BccI CCATC 3 cut(s) 388, 688, 872
BciT130I CCWGG 2 cut(s) 432, 637
BfaI CTAG 2 cut(s) 629, 737
BfmI CTRYAG 1 cut(s) 461
BfoI RGCGCY 2 cut(s) 92, 305
BglII AGATCT 1 cut(s) 582
BisI GCNGC 1 cut(s) 502
BlnI CCTAGG 1 cut(s) 628
BlsI GCNGC 1 cut(s) 503
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 2 cut(s) 432, 637
Bme18I GGWCC 2 cut(s) 388, 662
BmgT120I GGNCC 2 cut(s) 388, 662
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 2 cut(s) 432, 637
BmsI GCATC 5 cut(s) 54, 332, 662, 832, 967
BmtI GCTAGC 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 725, 757
Bpu14I TTCGAA 2 cut(s) 406, 951
BsaBI GATNNNNATC 1 cut(s) 981
BsaJI CCNNGG 2 cut(s) 628, 796
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 205, 358, 833
Bse3DI GCAATG 4 cut(s) 56, 511, 684, 932
Bse8I GATNNNNATC 1 cut(s) 981
BseBI CCWGG 2 cut(s) 432, 637
BseDI CCNNGG 2 cut(s) 628, 796
BseGI GGATG 4 cut(s) 677, 680, 883, 982
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 4 cut(s) 56, 511, 684, 932
BseMII CTCAG 1 cut(s) 1020
BseNI ACTGG 3 cut(s) 205, 358, 833
BseRI GAGGAG 1 cut(s) 555
BseXI GCAGC 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 2 cut(s) 255, 312
BsiEI CGRYCG 1 cut(s) 820
BslFI GGGAC 1 cut(s) 928
BslI CCNNNNNNNGG 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 928
BsmI GAATGC 3 cut(s) 339, 675, 927
BsnI GGCC 2 cut(s) 255, 312
Bsp119I TTCGAA 2 cut(s) 406, 951
Bsp143I GATC 3 cut(s) 290, 454, 582
BspANI GGCC 2 cut(s) 255, 312
BspCNI CTCAG 1 cut(s) 1019
BspLI GGNNCC 1 cut(s) 634
BspMAI CTGCAG 1 cut(s) 465
BspOI GCTAGC 1 cut(s) 740
BspT104I TTCGAA 2 cut(s) 406, 951
BsrDI GCAATG 4 cut(s) 56, 511, 684, 932
BsrI ACTGG 3 cut(s) 205, 358, 833
BssECI CCNNGG 2 cut(s) 628, 796
BssMI GATC 3 cut(s) 290, 454, 582
BssT1I CCWWGG 2 cut(s) 628, 796
Bst2UI CCWGG 2 cut(s) 432, 637
Bst4CI ACNGT 7 cut(s) 122, 226, 415, 707, 716, 945, 1042
BstAPI GCANNNNNTGC 3 cut(s) 73, 773, 891
BstBI TTCGAA 2 cut(s) 406, 951
BstC8I GCNNGC 2 cut(s) 738, 1003
BstDEI CTNAG 2 cut(s) 938, 1006
BstF5I GGATG 4 cut(s) 677, 680, 883, 982
BstH2I RGCGCY 2 cut(s) 92, 305
BstHHI GCGC 2 cut(s) 91, 304
BstKTI GATC 3 cut(s) 293, 457, 585
BstMBI GATC 3 cut(s) 290, 454, 582
BstMCI CGRYCG 1 cut(s) 820
BstMWI GCNNNNNNNGC 5 cut(s) 73, 336, 773, 891, 1032
BstNI CCWGG 2 cut(s) 432, 637
BstSCI CCNGG 2 cut(s) 430, 635
BstSFI CTRYAG 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BsuRI GGCC 2 cut(s) 255, 312
BtsCI GGATG 4 cut(s) 677, 680, 883, 982
BtsIMutI CAGTG 4 cut(s) 15, 127, 198, 712
Cac8I GCNNGC 2 cut(s) 738, 1003
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 2 cut(s) 91, 304
Cfr13I GGNCC 2 cut(s) 388, 662
Csp6I GTAC 3 cut(s) 466, 726, 897
CspCI CAANNNNNGTGG 4 cut(s) 688, 723, 953, 988
CviAII CATG 3 cut(s) 277, 649, 891
CviQI GTAC 3 cut(s) 466, 726, 897
DdeI CTNAG 2 cut(s) 938, 1006
DpnI GATC 3 cut(s) 292, 456, 584
DpnII GATC 3 cut(s) 290, 454, 582
DraI TTTAAA 1 cut(s) 1056
Eco130I CCWWGG 2 cut(s) 628, 796
Eco147I AGGCCT 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 388, 662
Eco47III AGCGCT 2 cut(s) 90, 303
Eco57I CTGAAG 2 cut(s) 555, 903
EcoRII CCWGG 2 cut(s) 430, 635
EcoT14I CCWWGG 2 cut(s) 628, 796
EcoT22I ATGCAT 3 cut(s) 69, 325, 677
ErhI CCWWGG 2 cut(s) 628, 796
FaeI CATG 3 cut(s) 280, 652, 894
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 1 cut(s) 928
FatI CATG 3 cut(s) 276, 648, 890
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 502
FokI GGATG 4 cut(s) 667, 684, 890, 989
Fsp4HI GCNGC 1 cut(s) 502
FspBI CTAG 2 cut(s) 629, 737
GlaI GCGC 2 cut(s) 90, 303
GluI GCNGC 1 cut(s) 502
HaeII RGCGCY 2 cut(s) 92, 305
HaeIII GGCC 2 cut(s) 255, 312
HhaI GCGC 2 cut(s) 91, 304
Hin1II CATG 3 cut(s) 280, 652, 894
Hin6I GCGC 2 cut(s) 89, 302
HinP1I GCGC 2 cut(s) 89, 302
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 3 cut(s) 107, 195, 379
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 1 cut(s) 424
Hpy8I GTNNAC 1 cut(s) 665
HpyCH4III ACNGT 7 cut(s) 122, 226, 415, 707, 716, 945, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 336, 773, 891, 1032
HpyF3I CTNAG 2 cut(s) 938, 1006
Hsp92II CATG 3 cut(s) 280, 652, 894
HspAI GCGC 2 cut(s) 89, 302
Kzo9I GATC 3 cut(s) 290, 454, 582
LmnI GCTCC 1 cut(s) 632
Lsp1109I GCAGC 1 cut(s) 513
LweI GCATC 5 cut(s) 54, 332, 662, 832, 967
MaeI CTAG 2 cut(s) 629, 737
MaeIII GTNAC 4 cut(s) 12, 125, 196, 592
MalI GATC 3 cut(s) 292, 456, 584
MboI GATC 3 cut(s) 290, 454, 582
MboII GAAGA 4 cut(s) 216, 231, 420, 449
MfeI CAATTG 2 cut(s) 78, 597
MflI RGATCY 1 cut(s) 582
MluCI AATT 8 cut(s) 78, 181, 316, 572, 597, 657, 778, 846
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 5 cut(s) 276, 536, 576, 609, 751
Mph1103I ATGCAT 3 cut(s) 69, 325, 677
MseI TTAA 6 cut(s) 189, 587, 782, 861, 1055, 1062
MslI CAYNNNNRTG 2 cut(s) 35, 670
MspR9I CCNGG 2 cut(s) 432, 637
MunI CAATTG 2 cut(s) 78, 597
Mva1269I GAATGC 3 cut(s) 339, 675, 927
MvaI CCWGG 2 cut(s) 432, 637
MwoI GCNNNNNNNGC 5 cut(s) 73, 336, 773, 891, 1032
NdeI CATATG 1 cut(s) 609
NdeII GATC 3 cut(s) 290, 454, 582
NheI GCTAGC 1 cut(s) 736
NlaIII CATG 3 cut(s) 280, 652, 894
NlaIV GGNNCC 1 cut(s) 634
NmuCI GTSAC 2 cut(s) 12, 196
NsiI ATGCAT 3 cut(s) 69, 325, 677
NspV TTCGAA 2 cut(s) 406, 951
PceI AGGCCT 1 cut(s) 312
PctI GAATGC 3 cut(s) 339, 675, 927
PfeI GAWTC 2 cut(s) 107, 379
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 503
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 430, 635
PspGI CCWGG 2 cut(s) 430, 635
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 2 cut(s) 388, 662
PstI CTGCAG 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 3 cut(s) 467, 727, 898
RsaNI GTAC 3 cut(s) 466, 726, 897
RseI CAYNNNNRTG 2 cut(s) 35, 670
SaqAI TTAA 6 cut(s) 189, 587, 782, 861, 1055, 1062
SatI GCNGC 1 cut(s) 502
Sau3AI GATC 3 cut(s) 290, 454, 582
Sau96I GGNCC 2 cut(s) 388, 662
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 2 cut(s) 432, 637
SetI ASST 8 cut(s) 528, 535, 620, 630, 738, 762, 802, 1024
SfaNI GCATC 5 cut(s) 54, 332, 662, 832, 967
SfcI CTRYAG 1 cut(s) 461
SfuI TTCGAA 2 cut(s) 406, 951
SinI GGWCC 2 cut(s) 388, 662
SmiMI CAYNNNNRTG 2 cut(s) 35, 670
Sse9I AATT 8 cut(s) 78, 181, 316, 572, 597, 657, 778, 846
SseBI AGGCCT 1 cut(s) 312
SspMI CTAG 2 cut(s) 629, 737
StuI AGGCCT 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 430, 635
StyI CCWWGG 2 cut(s) 628, 796
TaaI ACNGT 7 cut(s) 122, 226, 415, 707, 716, 945, 1042
TaqI TCGA 6 cut(s) 406, 528, 543, 743, 820, 951
TaqII GACCGA 1 cut(s) 806
TasI AATT 8 cut(s) 78, 181, 316, 572, 597, 657, 778, 846
TatI WGTACW 3 cut(s) 465, 725, 896
TfiI GAWTC 2 cut(s) 107, 379
Tru1I TTAA 6 cut(s) 189, 587, 782, 861, 1055, 1062
Tru9I TTAA 6 cut(s) 189, 587, 782, 861, 1055, 1062
TscAI CASTG 4 cut(s) 15, 127, 205, 712
TseFI GTSAC 2 cut(s) 12, 196
TseI GCWGC 1 cut(s) 501
Tsp45I GTSAC 2 cut(s) 12, 196
TspDTI ATGAA 3 cut(s) 17, 19, 502
TspGWI ACGGA 2 cut(s) 231, 1007
TspRI CASTG 4 cut(s) 15, 127, 205, 712
Van91I CCANNNNNTGG 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 388, 662
XapI RAATTY 2 cut(s) 181, 778
XmaJI CCTAGG 1 cut(s) 628
XspI CTAG 2 cut(s) 629, 737
ZrmI AGTACT 1 cut(s) 467
Zsp2I ATGCAT 3 cut(s) 69, 325, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.