pycom09g07240

Mitochondrial PGP phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005815
Physical Location & Seq
Forward (+)
5435889 .. 5442081
6193 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2676 bp
ATGCAGTCGACCTCAACAACTCCATTGCCAAAATGCTCTTACCCTCTCTCCCTTAACCACTTCCACTATCGACACCCTTGCAAAACCCTCAACTTCCTTCTCCTCCCCCCAAAACCCCAAACCCACTTCACCAACCACTGCGCCCTTTCCATCTCTCACACCCACGGTTGCAGCAGAGAGCACCAGAACCAGAGCCAGAATCACATCAATGGCGATTCCGACCCCTATCAGAACGATATGTTGTTTCTGCAATTGAACCCCTCAGGAGACACCGAAGACGCCGGTAACCAAAGCCCAGAAGCCCCAAACGACGAACGAGGGCGAGATCAAAGAGAGAGCGAAGCCGATAATTCCGAGGGACTTTCGACAAACATGTGGTGGGCAGACGTGAAAGCCGCATTGGGACAAAGGTTCAACTTTGGAGCATTAGTTCGTTCAGCCATGGTGCTCACGAAAGACAAGCACTTGGTGCTGCCTCATGTTTCTGTGCCTGATATAAGGTACATTGATTGGCCTGAGTTGCATAGAAGGGGTTTCAAGGGTGTTGTGTTCGATAAGGATAATACTATCACTGCACCTTATTCCTTGACGCTTTGGGGTCCTCTTGGGTCTTCATTGGAGCAGTGCAAATCAGTTTTTGGACCCGATATCGCGGTGTTCAGTAACTCGGCAGGGCTTTATGAGTATGACCATGATGGTTCAAAAGCTAGGGAGCTTGAAGGGGCTATTGGAATTAAAGTCATAAGACACAGAGTGAAGAAACCAGCCGGAACAGCTGAAGAGATCGAGAAGCATTTTGGTTGTAAATCGTCACACCTTATTATGGTGGGTGATCGGCCCTTAACTGATATTGTCTATGGTAATCAAAATGGATTTCTAACTATTTTAACCGAACCGTTGAGTCTCAACGAGGAACCATTCATTGTTAGGCAGGTGAGGAAACTAGAAACAGCCCTTGTGAATCATAGGTTTAAGAGAGGGTTAAAGCCCACCACTCATAGGCTGTTGCCTGACGGCAAGCAGTGTGTGAAACATCCGCCACCACTTTTGTACAGAGTGAGGCTTGATGGAAACCGGTTATCAGGAAACCTATCCGAAGACCTTGGTGTGTATCCAAACTTAAATTACATTGATTTGAGCCATAACAATTTCTATGTGGAACTTTCTCAGAACTGGGGGCAGTGCCAAAGTCTGCAAAGCTTGAAAATCTCCCACAACAGAATTTCCGGAGGGATTCCTCCTCAGCTTGGGGAAGCGGTGCAGCTGCGAGTACTTGACCTCTCCTCAAATTATCTAGTTGGGACTATTCCAAAGGAACTGGGGAGATTAGTATCACTGTTTGACCTTAACCTGGGTGGTAATGAACTTTCAGGCAGTATTCCGTGGGAAATTGGAATGATATCCAATCTTGAAGAAATTAACGTGGCAGCCAACAATCTCACTGGATTGTTTCCTGAGCAACCATATCAGTGCTCAAAGTTACTGAACATAAATTTGAGCGTAAATGGATTCATAGAGAGTCTTCCTTTTCAGATTGGGAGCTTACAATCTCTTCAGGTTCTTGATCTCAGTCATAATTCACTTGGAGGAGAGATACCGCCGCAGTTTGGAGAACTGGAAAGCTTAGAAGCAATGAATCTGTCTCACAATGAGCTCTCTGGTTCCTTCCCATCTAGTTTTGGTAATATGTTACACTTGACTGCTATTGACATATCCTACAATCAACTGGAGGCTCCTCTTCCGAACATCAAAGCCTTCAACGAGGCTCCAGTCAAAGCTTTAGAGAATAACAAAGGCTTGTGTGGTAATGCCACTGGCCTGAAGCCGTGCCAATCTACCATAAGAAACACAAAAAATAACAATAATAACATCATTATGATTGCAGCCCTCATTTCAGGAATTCTGTTTCTTGGGTTCATTGTAGTTCGGATTCTCTACATTCGGCGCCCACACCAGATTGTAGAAGAACAGCAAATGCCAAGAGAAACACAAACAGAAAATCTCTTTACAATTTGGAGTTATGATGGAAGATTGGTGTATGAGGACATCGTTGCAGCTACAGAGGAATTTGACTCTAAACGTTGTGTAGGAGCAGGGGGCCGTGCAAGTGTTTATAAAGCTAAGCTGCAAACAGGCCAGATTGTTGCGGTGAAGAAACTTCATACTCTGCAGGATGGTGGGATAACAAATCTGAAGGCTTTTGAGAGTGAGATTCCTGCTCTCTCAGAAATTCGCCATCGCAACATTGTCAAGCTCTATGGCTTTTGCACACATCCACGGCACTCATTCTTGGTTTACCAGTTTCTGGAAGGGGGAAGCTTAGAAAGGGTACTCAACAATGACAACGAAGCAACTATGTTTGAATGGATTTCAAGGATTAACCTTGTCAAAAGCATGGCGAATGCTTTATCCTACATGCACCATGACTGCTTGCCTCCAATTGTTCACCAAGACATGTCAAGCAAGAACATCTTGCTGGATTTGGAACATGCTGCTTACGTCTCTGATTTCGGCACAGCTAGAATTTTGAAGCCTGACTCTTCCAATTGGACTTCATTTGCAGGCACATTTGGATACACTACTCCAGGAGTGTTAACTTTAGAAGTGATCGATGGGAAAGCATCCGGGAGATCTCATGATCGCAGTTCTGTCCTCGACAACATCAACCGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 map00564 map01100
Pfam Domains
Protein Families
PGP_phosphatase

Protein Analysis

892

Amino Acids

99.19

Weight (kDa)

6.29

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGP_phosphatase PF09419 146 - 300 1.1e-42 Mitochondrial PGP phosphatase
LRR_8 PF13855 397 - 456 9.5e-07 Leucine rich repeat
LRR_8 PF13855 468 - 528 7.3e-07 Leucine rich repeat
LRR_14 PF23598 505 - 570 3.8e-07 Leucine-rich repeat region
LRR_8 PF13855 536 - 576 8.7e-06 Leucine rich repeat
Pkinase PF00069 691 - 867 2.6e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 693 - 848 1.2e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2115
AarI CACCTGC 1 cut(s) 922
Acc36I ACCTGC 1 cut(s) 922
AccB1I GGYRCC 1 cut(s) 1944
AccB7I CCANNNNNTGG 1 cut(s) 2305
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 653
AccIII TCCGGA 1 cut(s) 1226
AciI CCGC 7 cut(s) 396, 653, 1037, 1256, 1598, 1601, 2147
AclI AACGTT 1 cut(s) 2080
AcsI RAATTY 6 cut(s) 1221, 1492, 1899, 2066, 2229, 2523
AcuI CTGAAG 4 cut(s) 798, 1538, 1841, 2213
AcyI GRCGYC 2 cut(s) 279, 1945
AdeI CACNNNGTG 2 cut(s) 469, 754
AfaI GTAC 4 cut(s) 503, 1052, 1272, 2331
AfiI CCNNNNNNNGG 6 cut(s) 823, 999, 1247, 1351, 1607, 2305
AflIII ACRYGT 2 cut(s) 372, 2454
AgeI ACCGGT 1 cut(s) 1074
AjiI CACGTC 1 cut(s) 388
AjnI CCWGG 2 cut(s) 1350, 2584
AjuI GAANNNNNNNTTGG 4 cut(s) 383, 415, 711, 743
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
Alw21I GWGCWC 4 cut(s) 183, 450, 1475, 1656
Alw26I GTCTC 4 cut(s) 261, 908, 1647, 2506
AlwNI CAGNNNCTG 1 cut(s) 2305
Aor13HI TCCGGA 1 cut(s) 1226
AoxI GGCC 5 cut(s) 512, 836, 1816, 2098, 2134
ApeKI GCWGC 9 cut(s) 171, 472, 1261, 1264, 1427, 1883, 2054, 2125, 2492
ApoI RAATTY 6 cut(s) 1221, 1492, 1899, 2066, 2229, 2523
AsiGI ACCGGT 1 cut(s) 1074
AspLEI GCGC 2 cut(s) 143, 1947
AspS9I GGNCC 4 cut(s) 599, 641, 837, 2098
AsuC2I CCSGG 1 cut(s) 2626
AsuHPI GGTGA 5 cut(s) 121, 842, 946, 2161, 2438
AvaII GGWCC 2 cut(s) 599, 641
AxyI CCTNAGG 1 cut(s) 262
BanI GGYRCC 1 cut(s) 1944
BanII GRGCYC 1 cut(s) 1656
BbsI GAAGAC 4 cut(s) 282, 603, 1104, 1514
Bbv12I GWGCWC 4 cut(s) 183, 450, 1475, 1656
BbvCI CCTCAGC 1 cut(s) 1242
BbvI GCAGC 9 cut(s) 183, 459, 1251, 1273, 1439, 1895, 2066, 2112, 2479
BccI CCATC 8 cut(s) 158, 689, 1061, 1678, 2018, 2168, 2244, 2606
BceAI ACGGC 4 cut(s) 1030, 1810, 2085, 2294
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 2 cut(s) 1352, 2586
BciVI GTATCC 2 cut(s) 1122, 2567
BcnI CCSGG 1 cut(s) 2626
BcoDI GTCTC 4 cut(s) 261, 908, 1647, 2506
BfaI CTAG 5 cut(s) 708, 944, 1295, 1674, 2520
BfmI CTRYAG 2 cut(s) 2058, 2168
BfoI RGCGCY 1 cut(s) 1948
BfuAI ACCTGC 1 cut(s) 922
BfuI GTATCC 2 cut(s) 1122, 2567
BglII AGATCT 1 cut(s) 2630
BlpI GCTNAGC 1 cut(s) 2121
BmcAI AGTACT 1 cut(s) 1272
Bme1390I CCNGG 3 cut(s) 1352, 2586, 2626
Bme18I GGWCC 2 cut(s) 599, 641
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 4 cut(s) 599, 641, 837, 2098
BmiI GGNNCC 8 cut(s) 600, 643, 915, 1663, 1734, 1767, 1946, 2099
BmrFI CCNGG 3 cut(s) 1352, 2586, 2626
BmrI ACTGGG 2 cut(s) 1183, 1328
BmsI GCATC 1 cut(s) 2630
BmuI ACTGGG 2 cut(s) 1183, 1328
BpiI GAAGAC 4 cut(s) 282, 603, 1104, 1514
BpmI CTGGAG 3 cut(s) 1748, 1752, 2568
Bpu10I CCTNAGC 2 cut(s) 1242, 1455
Bpu1102I GCTNAGC 1 cut(s) 2121
BpuMI CCSGG 1 cut(s) 2626
Bsa29I ATCGAT 1 cut(s) 2610
BsaBI GATNNNNATC 1 cut(s) 1330
BsaHI GRCGYC 2 cut(s) 279, 1945
BsaJI CCNNGG 7 cut(s) 163, 354, 441, 1102, 1351, 1382, 2276
BsaWI WCCGGW 2 cut(s) 1074, 1226
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 414, 444
Bsc4I CCNNNNNNNGG 6 cut(s) 823, 999, 1247, 1351, 1607, 2305
Bse118I RCCGGY 2 cut(s) 281, 1074
Bse1I ACTGG 8 cut(s) 1178, 1323, 1447, 1620, 1731, 1769, 1819, 2299
Bse21I CCTNAGG 1 cut(s) 262
Bse3DI GCAATG 2 cut(s) 23, 1638
Bse8I GATNNNNATC 1 cut(s) 1330
BseAI TCCGGA 1 cut(s) 1226
BseBI CCWGG 2 cut(s) 1352, 2586
BseCI ATCGAT 1 cut(s) 2610
BseDI CCNNGG 7 cut(s) 163, 354, 441, 1102, 1351, 1382, 2276
BseGI GGATG 4 cut(s) 1033, 2179, 2272, 2621
BseJI GATNNNNATC 1 cut(s) 1330
BseLI CCNNNNNNNGG 6 cut(s) 823, 999, 1247, 1351, 1607, 2305
BseMI GCAATG 2 cut(s) 23, 1638
BseMII CTCAG 7 cut(s) 276, 507, 1181, 1256, 1446, 1582, 2238
BseNI ACTGG 8 cut(s) 1178, 1323, 1447, 1620, 1731, 1769, 1819, 2299
BseRI GAGGAG 5 cut(s) 92, 1230, 1273, 1602, 1725
BseXI GCAGC 9 cut(s) 183, 459, 1251, 1273, 1439, 1895, 2066, 2112, 2479
BsgI GTGCAG 2 cut(s) 558, 1280
Bsh1236I CGCG 1 cut(s) 653
BshFI GGCC 5 cut(s) 514, 838, 1818, 2100, 2136
BshNI GGYRCC 1 cut(s) 1944
BshTI ACCGGT 1 cut(s) 1074
BshVI ATCGAT 1 cut(s) 2610
BsiHKAI GWGCWC 4 cut(s) 183, 450, 1475, 1656
BsiSI CCGG 5 cut(s) 282, 768, 1075, 1227, 2625
BslFI GGGAC 3 cut(s) 372, 417, 1315
BslI CCNNNNNNNGG 6 cut(s) 823, 999, 1247, 1351, 1607, 2305
BsmAI GTCTC 4 cut(s) 261, 908, 1647, 2506
BsmBI CGTCTC 1 cut(s) 2506
BsmFI GGGAC 3 cut(s) 372, 417, 1315
BsmI GAATGC 1 cut(s) 2407
BsnI GGCC 5 cut(s) 514, 838, 1818, 2100, 2136
Bsp1286I GDGCHC 4 cut(s) 183, 450, 1475, 1656
Bsp13I TCCGGA 1 cut(s) 1226
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
Bsp1720I GCTNAGC 1 cut(s) 2121
Bsp19I CCATGG 1 cut(s) 441
BspACI CCGC 7 cut(s) 396, 653, 1037, 1256, 1598, 1601, 2147
BspANI GGCC 5 cut(s) 514, 838, 1818, 2100, 2136
BspCNI CTCAG 7 cut(s) 275, 508, 1180, 1255, 1447, 1581, 2237
BspDI ATCGAT 1 cut(s) 2610
BspEI TCCGGA 1 cut(s) 1226
BspFNI CGCG 1 cut(s) 653
BspHI TCATGA 1 cut(s) 2635
BspLI GGNNCC 8 cut(s) 600, 643, 915, 1663, 1734, 1767, 1946, 2099
BspMAI CTGCAG 1 cut(s) 2172
BspMI ACCTGC 1 cut(s) 922
BspT107I GGYRCC 1 cut(s) 1944
BsrDI GCAATG 2 cut(s) 23, 1638
BsrFI RCCGGY 2 cut(s) 281, 1074
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 8 cut(s) 1178, 1323, 1447, 1620, 1731, 1769, 1819, 2299
BssAI RCCGGY 2 cut(s) 281, 1074
BssECI CCNNGG 7 cut(s) 163, 354, 441, 1102, 1351, 1382, 2276
BssMI GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
BssNI GRCGYC 2 cut(s) 279, 1945
BssT1I CCWWGG 2 cut(s) 441, 1102
Bst2UI CCWGG 2 cut(s) 1352, 2586
Bst4CI ACNGT 4 cut(s) 167, 897, 1338, 2669
Bst6I CTCTTC 4 cut(s) 774, 1557, 1743, 2545
BstACI GRCGYC 2 cut(s) 279, 1945
BstAPI GCANNNNNTGC 1 cut(s) 469
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 3 cut(s) 1019, 2432, 2563
BstDSI CCRYGG 4 cut(s) 163, 441, 1382, 2276
BstEII GGTNACC 1 cut(s) 284
BstF5I GGATG 4 cut(s) 1033, 2179, 2272, 2621
BstFNI CGCG 1 cut(s) 653
BstH2I RGCGCY 1 cut(s) 1948
BstHHI GCGC 2 cut(s) 143, 1947
BstKTI GATC 7 cut(s) 328, 786, 835, 1567, 2610, 2633, 2641
BstMAI GTCTC 4 cut(s) 261, 908, 1647, 2506
BstMBI GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
BstMWI GCNNNNNNNGC 3 cut(s) 469, 520, 773
BstNI CCWGG 2 cut(s) 1352, 2586
BstNSI RCATGY 4 cut(s) 376, 2419, 2458, 2492
BstPI GGTNACC 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 1350, 2584, 2624
BstSFI CTRYAG 2 cut(s) 2058, 2168
BstUI CGCG 1 cut(s) 653
BstV1I GCAGC 9 cut(s) 183, 459, 1251, 1273, 1439, 1895, 2066, 2112, 2479
BstV2I GAAGAC 4 cut(s) 282, 603, 1104, 1514
BstX2I RGATCY 1 cut(s) 2630
BstYI RGATCY 1 cut(s) 2630
Bsu15I ATCGAT 1 cut(s) 2610
Bsu36I CCTNAGG 1 cut(s) 262
BsuI GTATCC 2 cut(s) 1122, 2567
BsuRI GGCC 5 cut(s) 514, 838, 1818, 2100, 2136
BsuTUI ATCGAT 1 cut(s) 2610
BtgI CCRYGG 4 cut(s) 163, 441, 1382, 2276
BtgZI GCGATG 1 cut(s) 2222
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 4 cut(s) 1033, 2179, 2272, 2621
BtsI GCAGTG 5 cut(s) 136, 570, 629, 1028, 1187
BtsIMutI CAGTG 9 cut(s) 136, 570, 629, 1028, 1187, 1334, 1440, 1475, 1812
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 3 cut(s) 1019, 2432, 2563
CaiI CAGNNNCTG 1 cut(s) 2305
CciI TCATGA 1 cut(s) 2635
CfoI GCGC 2 cut(s) 143, 1947
Cfr10I RCCGGY 2 cut(s) 281, 1074
Cfr13I GGNCC 4 cut(s) 599, 641, 837, 2098
ClaI ATCGAT 1 cut(s) 2610
CseI GACGC 2 cut(s) 287, 598
Csp6I GTAC 4 cut(s) 502, 1051, 1271, 2330
CspAI ACCGGT 1 cut(s) 1074
CspCI CAANNNNNGTGG 2 cut(s) 1029, 1064
CviQI GTAC 4 cut(s) 502, 1051, 1271, 2330
DinI GGCGCC 1 cut(s) 1946
DpnI GATC 7 cut(s) 327, 785, 834, 1566, 2609, 2632, 2640
DpnII GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
DraIII CACNNNGTG 2 cut(s) 469, 754
Eam1104I CTCTTC 4 cut(s) 774, 1557, 1743, 2545
EarI CTCTTC 4 cut(s) 774, 1557, 1743, 2545
EciI GGCGGA 1 cut(s) 1026
Ecl136II GAGCTC 1 cut(s) 1654
Eco130I CCWWGG 2 cut(s) 441, 1102
Eco24I GRGCYC 1 cut(s) 1656
Eco32I GATATC 2 cut(s) 649, 1401
Eco47I GGWCC 2 cut(s) 599, 641
Eco53kI GAGCTC 1 cut(s) 1654
Eco57I CTGAAG 4 cut(s) 798, 1538, 1841, 2213
Eco81I CCTNAGG 1 cut(s) 262
Eco91I GGTNACC 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 1654
EcoO109I RGGNCCY 1 cut(s) 599
EcoO65I GGTNACC 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 1899
EcoRII CCWGG 2 cut(s) 1350, 2584
EcoRV GATATC 2 cut(s) 649, 1401
EcoT14I CCWWGG 2 cut(s) 441, 1102
EcoT38I GRGCYC 1 cut(s) 1656
EgeI GGCGCC 1 cut(s) 1946
EheI GGCGCC 1 cut(s) 1946
ErhI CCWWGG 2 cut(s) 441, 1102
Esp3I CGTCTC 1 cut(s) 2506
FalI AAGNNNNNCTT 2 cut(s) 2456, 2488
FaqI GGGAC 3 cut(s) 372, 417, 1315
FblI GTMKAC 1 cut(s) 8
FokI GGATG 4 cut(s) 1020, 2186, 2259, 2608
FriOI GRGCYC 1 cut(s) 1656
FspBI CTAG 5 cut(s) 708, 944, 1295, 1674, 2520
GlaI GCGC 2 cut(s) 142, 1946
GsuI CTGGAG 3 cut(s) 1748, 1752, 2568
HaeII RGCGCY 1 cut(s) 1948
HaeIII GGCC 5 cut(s) 514, 838, 1818, 2100, 2136
HapII CCGG 5 cut(s) 282, 768, 1075, 1227, 2625
HgaI GACGC 2 cut(s) 287, 598
HhaI GCGC 2 cut(s) 143, 1947
Hin1I GRCGYC 2 cut(s) 279, 1945
Hin6I GCGC 2 cut(s) 141, 1945
HinP1I GCGC 2 cut(s) 141, 1945
HincII GTYRAC 2 cut(s) 9, 2595
HindII GTYRAC 2 cut(s) 9, 2595
HindIII AAGCTT 4 cut(s) 1198, 1621, 1776, 2317
HpaI GTTAAC 1 cut(s) 2595
HpaII CCGG 5 cut(s) 282, 768, 1075, 1227, 2625
HphI GGTGA 5 cut(s) 121, 842, 946, 2161, 2438
Hpy166II GTNNAC 4 cut(s) 9, 2296, 2446, 2595
Hpy8I GTNNAC 4 cut(s) 9, 2296, 2446, 2595
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 7 cut(s) 107, 522, 713, 1675, 1765, 2188, 2303
HpyCH4III ACNGT 4 cut(s) 167, 897, 1338, 2669
HpyCH4IV ACGT 4 cut(s) 387, 1422, 2080, 2499
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 520, 773
HpySE526I ACGT 4 cut(s) 387, 1422, 2080, 2499
Hsp92I GRCGYC 2 cut(s) 279, 1945
HspAI GCGC 2 cut(s) 141, 1945
KasI GGCGCC 1 cut(s) 1944
Kpn2I TCCGGA 1 cut(s) 1226
KspAI GTTAAC 1 cut(s) 2595
Kzo9I GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
LmnI GCTCC 7 cut(s) 422, 619, 712, 1539, 1738, 1771, 2090
Lsp1109I GCAGC 9 cut(s) 183, 459, 1251, 1273, 1439, 1895, 2066, 2112, 2479
LweI GCATC 1 cut(s) 2630
MaeI CTAG 5 cut(s) 708, 944, 1295, 1674, 2520
MaeII ACGT 4 cut(s) 387, 1422, 2080, 2499
MaeIII GTNAC 5 cut(s) 284, 662, 810, 1479, 1689
MalI GATC 7 cut(s) 327, 785, 834, 1566, 2609, 2632, 2640
MboI GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
MfeI CAATTG 3 cut(s) 251, 2439, 2545
MflI RGATCY 1 cut(s) 2630
MhlI GDGCHC 4 cut(s) 183, 450, 1475, 1656
Mly113I GGCGCC 1 cut(s) 1945
MlyI GAGTC 4 cut(s) 910, 1528, 2066, 2531
MmeI TCCRAC 1 cut(s) 243
MroI TCCGGA 1 cut(s) 1226
MslI CAYNNNNRTG 3 cut(s) 207, 1468, 1874
MspA1I CMGCKG 2 cut(s) 776, 1264
MspI CCGG 5 cut(s) 282, 768, 1075, 1227, 2625
MspR9I CCNGG 3 cut(s) 1352, 2586, 2626
MunI CAATTG 3 cut(s) 251, 2439, 2545
Mva1269I GAATGC 1 cut(s) 2407
MvaI CCWGG 2 cut(s) 1352, 2586
MvnI CGCG 1 cut(s) 653
MwoI GCNNNNNNNGC 3 cut(s) 469, 520, 773
NarI GGCGCC 1 cut(s) 1945
NciI CCSGG 1 cut(s) 2626
NcoI CCATGG 1 cut(s) 441
NdeII GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
NlaIV GGNNCC 8 cut(s) 600, 643, 915, 1663, 1734, 1767, 1946, 2099
NmeAIII GCCGAG 1 cut(s) 647
NmuCI GTSAC 1 cut(s) 810
NspI RCATGY 4 cut(s) 376, 2419, 2458, 2492
PagI TCATGA 1 cut(s) 2635
PaqCI CACCTGC 1 cut(s) 922
PciI ACATGT 2 cut(s) 372, 2454
PctI GAATGC 1 cut(s) 2407
PfeI GAWTC 8 cut(s) 199, 215, 961, 1234, 1509, 1636, 1930, 2212
PflMI CCANNNNNTGG 1 cut(s) 2305
PfoI TCCNGGA 2 cut(s) 2584, 2624
PinAI ACCGGT 1 cut(s) 1074
PleI GAGTC 4 cut(s) 909, 1527, 2066, 2531
PluTI GGCGCC 1 cut(s) 1948
PpsI GAGTC 4 cut(s) 909, 1527, 2066, 2531
PpuMI RGGWCCY 1 cut(s) 599
PscI ACATGT 2 cut(s) 372, 2454
PsiI TTATAA 1 cut(s) 2115
Psp124BI GAGCTC 1 cut(s) 1656
Psp1406I AACGTT 1 cut(s) 2080
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 1350, 2584
PspEI GGTNACC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 1350, 2584
PspN4I GGNNCC 8 cut(s) 600, 643, 915, 1663, 1734, 1767, 1946, 2099
PspPI GGNCC 4 cut(s) 599, 641, 837, 2098
PspPPI RGGWCCY 1 cut(s) 599
PstI CTGCAG 1 cut(s) 2172
PstNI CAGNNNCTG 1 cut(s) 2305
PsuI RGATCY 1 cut(s) 2630
PvuII CAGCTG 2 cut(s) 776, 1264
RsaI GTAC 4 cut(s) 503, 1052, 1272, 2331
RsaNI GTAC 4 cut(s) 502, 1051, 1271, 2330
RseI CAYNNNNRTG 3 cut(s) 207, 1468, 1874
SacI GAGCTC 1 cut(s) 1656
SalI GTCGAC 1 cut(s) 7
Sau3AI GATC 7 cut(s) 325, 783, 832, 1564, 2607, 2630, 2638
Sau96I GGNCC 4 cut(s) 599, 641, 837, 2098
ScaI AGTACT 1 cut(s) 1272
SchI GAGTC 4 cut(s) 910, 1528, 2066, 2531
ScrFI CCNGG 3 cut(s) 1352, 2586, 2626
SduI GDGCHC 4 cut(s) 183, 450, 1475, 1656
SfaNI GCATC 1 cut(s) 2630
SfcI CTRYAG 2 cut(s) 2058, 2168
SfoI GGCGCC 1 cut(s) 1946
SinI GGWCC 2 cut(s) 599, 641
SmiMI CAYNNNNRTG 3 cut(s) 207, 1468, 1874
SsiI CCGC 7 cut(s) 396, 653, 1037, 1256, 1598, 1601, 2147
SspDI GGCGCC 1 cut(s) 1944
SspMI CTAG 5 cut(s) 708, 944, 1295, 1674, 2520
SstI GAGCTC 1 cut(s) 1656
StyD4I CCNGG 3 cut(s) 1350, 2584, 2624
StyI CCWWGG 2 cut(s) 441, 1102
TaaI ACNGT 4 cut(s) 167, 897, 1338, 2669
TaiI ACGT 4 cut(s) 390, 1425, 2083, 2502
TaqI TCGA 7 cut(s) 8, 70, 365, 552, 786, 2610, 2655
TatI WGTACW 2 cut(s) 1050, 1270
TauI GCSGC 2 cut(s) 398, 1603
TfiI GAWTC 8 cut(s) 199, 215, 961, 1234, 1509, 1636, 1930, 2212
TscAI CASTG 9 cut(s) 143, 577, 629, 1028, 1187, 1341, 1447, 1475, 1819
TseFI GTSAC 1 cut(s) 810
TseI GCWGC 9 cut(s) 171, 472, 1261, 1264, 1427, 1883, 2054, 2125, 2492
Tsp45I GTSAC 1 cut(s) 810
TspDTI ATGAA 8 cut(s) 603, 910, 1377, 1501, 1649, 1906, 2150, 2544
TspGWI ACGGA 1 cut(s) 1371
TspRI CASTG 9 cut(s) 143, 577, 629, 1028, 1187, 1341, 1447, 1475, 1819
Van91I CCANNNNNTGG 1 cut(s) 2305
VpaK11BI GGWCC 2 cut(s) 599, 641
XapI RAATTY 6 cut(s) 1221, 1492, 1899, 2066, 2229, 2523
XceI RCATGY 4 cut(s) 376, 2419, 2458, 2492
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 5 cut(s) 708, 944, 1295, 1674, 2520
ZrmI AGTACT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.