pycom09g12060
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002725
Physical Location & Seq
Reverse (-)
10384119 .. 10393562
9444 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4494 bp
ATGGCGGGAACAGAGAAAGCCAAAGGTTCAGAGCTACATCATAAAAAGCGCCATAGCCGCAGCGGAAGCATAAGCAGGAGCCTGAGCAGGGCAAGTTGGCGCATGGAAGAAGTATTTGCGACTGCCACGCACTCGAGAACAAGCAGCCAAGCGGACGAAGATGAAGAAGATCTGAAATGGGCTGCCATTGAGAAACTACCCACGTATGATCGGCTAAGAACAAGCATCATCAAGTCCCTTGGGGAAACTGAGCCTCAAGGAGTACATAAGCAAGTAGACGTTCTAAAGCTCGACATGAACGACCGTCAGAATTTCATTCAAAGGATTTTCAAGGTTGCAGAGGAAGATAATGAGACGTTCTTGAAAAAGTTCAGAAGTAGACTAGATAAGGTTGGGATCAGACTTCCCACAGTTGAAGTAAGGTATGAGCATTTGACAATTGAAGCTGATTGCCATGTTGGAACAAGAGCTCTTCCCACCCTCCCAAATGTTGCTCGGAACATCGCAGAATCAGCAATTGGCTTACTTGGGATTAAATTGACTAAGCAAACAAAACTAACAATTCTCAAAGATGTCTCTGGCATTATTAAACCTTCAAGGATGGCCCTTTTATTAGGTCCTCCATCCTCGGGGAAAACTACCCTTTTGCTGGCTTTAGCTGGGAAGATGGATCCAAGCTTGAAGGTTAAAGGAGAGGTGACTTACAATGGTCACAGGCTGAATGAGTTTGTGCCACGGAAGACATCTGCATATATTAGCCAAAATGAGGTTCATATAGGAAATATGACGGTCAAAGAAACTCTAGATTTTTCAGCAAGGTGTCAAGGGGTTGGGACTCGATATGAACTTCTTTCTGAGCTTGCTAGAAGAGAAAAGGCCGCTGGGATATATCCAGAGCCAGAAGTAGACCTTTTCATGAAGGCTACTTCAATGGGAGGACTTGAGAGCAGTCTCATCACTGATTATATTCTCAAACTTTTGGGGCTTGACATATGTAAGGATACACTTGTTGGAGATGACATGCAGAGAGGGATATCTGGTGGCCAGAAAAAACGAGTAACCACAGGTGAGATGATTGTTGGACCTACTAAAACATTGTTGATGGATGAGATCTCAACAGGCCTTGATAGCTCCACGACGTTCCAAATCGTGAAGTGCCTACAACAAATTGTACATATCACTGAGGCTACGATCTTGGTGTCTCTACTTCAACCTGCTCCTGAGACATTCGATCTTTTTGATGATATCATTCTATTATCACAAGGTCAGATCGTCTACCAGGGACCACGTGAGAACATATTAGAGTTCTTTGAGACATGTGGATTTCGATGCCCTGAGAGAAAAGGAACTGCCGATTTTTTACAAGAGGTTACCTCAAGAAAAGACCAGGAGCAGTATTGGGCGGACAGAAGGACTCCATACAGATATGTATCGGTCACAGAATTTGCAAACCGGTTCAAGCGTTTCCATGTGGGCATGAGGATAGCGGATGAGCTCTCAATCCCATTCGACAAGGCTCAAAGCCACAAAGCAGCTCTTGTATTCAAAACATATTCAATGCCCAAAATGCAGCTTCTCAAAGCATGTTTCGACAAAGAATGGCTGCTCATTCAAAGGAACTCATTTATTTACATTGTCAAGAGTGTCCAAATTATAATAGCGGCATTCATAGCGTCTACAGTATTTTTGAGGACCGAACTGAACACTCGAAATGAAGATGATGGAGCACTCTATGTTGGTGCACTTATCTTTGTCCTAATCGTAAACATGTTTAATGGGTTTGCTGAGCTTTCATTGACTATTGCAAGACTTCCTGTGTTTTACAAGCATAGAGACCTTCTTTTCCACCCTGCTTGGACTTTCACTCTTCCATCTGTCCTGCTTGGGATTCCTATATCCATCATCGAATCTACTGTTTGGACGGCCTTTACATATTACGTGATTGGATTTGCACCGGAAGCTAGCAGGTTTTTCAAGCAATTATTATTGGTATTTCTGATCGATCAAATGGCATCGTCGCTATTTAGGCTTATTGCAGGAGTTAGCAGGACCATGATCATATCAAACACTGGCGGGACTCTCACTCTTCTAGTTGTTTTCTTGCTTGGAGGTTTCATAATTCCAAAAGGTCAAATTCCAAATTGGTGGACGTGGGCATACTGGATTTCACCTTTGTCATATGGCTTCAATGCTATCTCTGTAAATGAAATGTATGCTCCGAGGTGGATGAACAAACGGGCTTTAGATAATGTTACCAGCGTGGGTGAGGCAGTGCTTAATAACTTTGATGTTTACCACAACAAAAACTGGTTTTGGATTGGTTCTGGCGCTCTTCTGGGGTTTATTATTCTATTCAACTCCCTTTATACGCTCTCTCTTATATACCTAAATGCTCCTGGAAAGCCACAAGCCATCATATCTGAAGAAGCAGCAAACAAAATGGAGCTAGACCAAGAAGAATCAAATGAAGAGCAAAGACTGAGAAGACCACCATCCAAGAAAGTTTCATTGCCTCGATCATTATCATCTACTGATGGAAACAATACAAGAGAAATGGAAGTCAGACAAATGAGCAGTCGATCTAGCTCTAGTGGACTACATAGAAATGCTGATTTGTCTCTCAAAGTCTCCAGTGGCGTTGCCCCCAAGAGAGGAATGGTTCTACCTTTCACTCCTCTTGCAATGTCCTTCGACAGTGTTAATTATTTCGTGGACATGCCAGCTGAAATGAAGGAGGAAGGAGTAGCAGAGGACAGGCTGCAACTACTTCGTGAAGTAACTGGTGCATTTAGGCCTGGGATCTTGACTGCCCTAATGGGCGTCAGTGGGGCTGGGAAAACGACTTTGATGGATGTCTTAGCAGGAAGAAAGACTGGAGGTTACATTGAAGGTGACATTAGAATTTCCGGGTACCCTAAGAAGCAAGAAACCTTTGCAAGAATTTCTGGTTACTGTGAACAAACTGATATCCACTCACCCCAAGTTACTATAAAAGAATCGTTGATTTACTCAGCTTTCCTTAGGCTCCCTAAAGAAGTCAACAACGAGGAAAAGATGATTTTTGTAGGTCAAGTGATGGAACTGGTTGAGCTAGACAATCTCAAAGATGCTTTAGTAGGGCTTCCCGGAGTTTCGGGGTTGTCAACAGAACAAAGAAAGAGGTTAACGATTGCTGTTGAGCTTGTTGCCAATCCCTCAATCATTTTCATGGATGAACCAACATCAGGTCTTGATGCAAGGGCAGCTGCCATTGTTATGAGGACTGTTAGAAATACAGTGGACACCGGGAGAACAGTTGTTTGCACAATTCATCAGCCTAGCATAGATATATTTGAGGCATTTGATGAGCTGCTACTGATGAAGAGAGGAGGGCAAGTTATCTACTTAGGACCACTGGGTCACAATTCTTACAAGATTGTTGAATATTTTGAGGCGGTTCCTGGCGTGCCAAAATTCAAAGAGAAGTATAATCCAGCTACATGGATGCTTGAGGCAAGTTCAACGTCAATTGAGGTCCGGCTTGGAATGGACTTTGCTCAATACTACAAATCATCTGCCTTGTATCAGAGAAACAAAGCTTTAGTAAATGAGTTGAGCACACCTCCAGCAGGAGCAAACGACCTCTATTTCCCCACTCAGTTTTCTCAGTCGTCATGGAAGCAATTCCAGTCTTGCCTCTGGAAGCAGTGGTGGACATATTGGAGAAGTCCTGACTATAACCTTGTCAGATTGTTTTTTACCTTGGCAGCTGCCCTGCTCCTTGGAAGTATTTTCTGGGATGTTGGCTCAAAAAGGGAAAGCAGTTCTGATTTGACCATGGTTATCGGGGCTATGTACGCTGCTGTTCTTTTCGTCGGAGTTGACAACTGCGCAACGGTGCAGCCAATTGTCGCCATTGAAAGAACGGTTTTCTATCGCGAAAGAGCTGCTGGGATGTACTCTGCATTACCTTATGCACTGGCACAGGTCATTATTGAAATACCATATGTGTTTATTCAGACCACTTACTATACACTGATAGTGTATGCTATGGTGAGCTTCCAATGGAAAGTAGAAAAATTCTTCTGGTTCTTCTTTATCAACTTCTTCTCCTTCCTTTACTTCACATATTACGGCATGATGACCGTTTCCATCACACCGAACCACCAAGTAGCAGCCATATTCGCCGCAGCTTTCTATTCAATCTTCAATCTCTTCTCCGGTTTCTTCATTCCAAGACCAAAAATACCCAAATGGTGGGTTTGGTACTACTGGATATGTCCCGTGGCATGGACGGTGTATGGATTGATTGTGTCACAGTACAGCGACATTGAGGAGACCATTACAGCACCAGGGATTACACCTGACCCAACTGTGAAATGGTACATAGAAGACCACTTCGGATTTAAATCAAATTTCATGGGACCAGTGGCTGGAGTTCTAGTTGGATTCGCAGTCTTCTTTGCTTTCATGTTTGCCTTCTGTATTAGGACACTCAACTTCCAAGTTCGGTAG

Protein Analysis

1498

Amino Acids

169.02

Weight (kDa)

8.49

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 114 - 164 1.4e-08 ABC-transporter N-terminal
ABC_tran PF00005 189 - 370 4.1e-16 ABC transporter
ABC2_membrane PF01061 525 - 736 1.5e-52 ABC-2 type transporter
PDR_assoc PF08370 742 - 806 4.2e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 924 - 1075 5.8e-19 ABC transporter
ABC2_membrane PF01061 1221 - 1434 4.3e-62 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1655
AasI GACNNNNNNGTC 1 cut(s) 3396
Acc16I TGCGCA 1 cut(s) 3871
Acc36I ACCTGC 2 cut(s) 1222, 1956
Acc65I GGTACC 1 cut(s) 2910
AccB1I GGYRCC 1 cut(s) 2910
AccB7I CCANNNNNTGG 2 cut(s) 4236, 4412
AccI GTMKAC 5 cut(s) 276, 379, 906, 1275, 1676
AccII CGCG 1 cut(s) 3918
AclWI GGATC 4 cut(s) 404, 665, 678, 2807
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 8 cut(s) 310, 1442, 2133, 2901, 2940, 3452, 4058, 4393
AcuI CTGAAG 1 cut(s) 2442
AcvI CACGTG 1 cut(s) 1289
AcyI GRCGYC 1 cut(s) 2820
AfaI GTAC 8 cut(s) 264, 1173, 2912, 3836, 3938, 4247, 4301, 4364
AflIII ACRYGT 2 cut(s) 1316, 1767
AgeI ACCGGT 1 cut(s) 1452
AjiI CACGTC 1 cut(s) 2151
AjnI CCWGG 6 cut(s) 1278, 1386, 2395, 2794, 3439, 4330
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 753, 785
Alw21I GWGCWC 5 cut(s) 472, 1497, 1729, 1744, 3599
Alw44I GTGCAC 1 cut(s) 1740
AlwI GGATC 4 cut(s) 404, 665, 678, 2807
AlwNI CAGNNNCTG 1 cut(s) 4412
Ama87I CYCGRG 2 cut(s) 133, 628
AoxI GGCC 6 cut(s) 603, 876, 1042, 1120, 1923, 2792
ApaLI GTGCAC 1 cut(s) 1740
ApoI RAATTY 8 cut(s) 310, 1442, 2133, 2901, 2940, 3452, 4058, 4393
ArsI GACNNNNNNTTYG 2 cut(s) 2560, 2592
AsiGI ACCGGT 1 cut(s) 1452
Asp700I GAANNNNTTC 2 cut(s) 368, 3662
Asp718I GGTACC 1 cut(s) 2910
AspLEI GCGC 4 cut(s) 51, 102, 2330, 3872
AspS9I GGNCC 9 cut(s) 604, 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
AsuC2I CCSGG 3 cut(s) 2908, 3126, 3286
AsuHPI GGTGA 7 cut(s) 709, 1079, 2160, 2276, 2903, 2967, 4045
AsuNHI GCTAGC 1 cut(s) 1961
AvaI CYCGRG 2 cut(s) 133, 628
AvaII GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
AxyI CCTNAGG 1 cut(s) 3020
BaeGI GKGCMC 1 cut(s) 1744
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BalI TGGCCA 1 cut(s) 1044
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 1 cut(s) 2910
BanII GRGCYC 2 cut(s) 472, 1497
BbrPI CACGTG 1 cut(s) 1289
BbsI GAAGAC 4 cut(s) 746, 2491, 4377, 4429
Bbv12I GWGCWC 5 cut(s) 472, 1497, 1729, 1744, 3599
BceAI ACGGC 2 cut(s) 1938, 4129
BcgI CGANNNNNNTGC 4 cut(s) 2750, 2784, 3908, 3942
BciT130I CCWGG 6 cut(s) 1280, 1388, 2397, 2796, 3441, 4332
BciVI GTATCC 1 cut(s) 994
BclI TGATCA 1 cut(s) 2055
BcnI CCSGG 3 cut(s) 2908, 3126, 3286
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 2 cut(s) 52, 2331
BfuAI ACCTGC 2 cut(s) 1222, 1956
BfuI GTATCC 1 cut(s) 994
BglII AGATCT 2 cut(s) 169, 1110
BlpI GCTNAGC 1 cut(s) 1785
Bme1390I CCNGG 9 cut(s) 1280, 1388, 2397, 2796, 2908, 3126, 3286, 3441, 4332
Bme18I GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
BmeT110I CYCGRG 2 cut(s) 133, 628
BmgBI CACGTC 1 cut(s) 2151
BmgT120I GGNCC 9 cut(s) 604, 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
BmiI GGNNCC 7 cut(s) 80, 672, 1284, 2912, 3026, 3438, 4404
BmrFI CCNGG 9 cut(s) 1280, 1388, 2397, 2796, 2908, 3126, 3286, 3441, 4332
BmrI ACTGGG 1 cut(s) 3404
BmsI GCATC 6 cut(s) 234, 1319, 2021, 3097, 3223, 3474
BmtI GCTAGC 1 cut(s) 1965
BmuI ACTGGG 1 cut(s) 3404
BpiI GAAGAC 4 cut(s) 746, 2491, 4377, 4429
BplI GAGNNNNNCTC 4 cut(s) 1358, 1390, 3586, 3618
BpmI CTGGAG 4 cut(s) 2614, 2895, 3588, 4434
Bpu10I CCTNAGC 1 cut(s) 83
Bpu1102I GCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 4 cut(s) 240, 962, 1360, 3509
BpuMI CCSGG 3 cut(s) 2908, 3126, 3286
Bsa29I ATCGAT 1 cut(s) 2001
BsaAI YACGTR 3 cut(s) 204, 1289, 1939
BsaBI GATNNNNATC 3 cut(s) 1429, 2521, 4387
BsaHI GRCGYC 1 cut(s) 2820
BsaI GGTCTC 2 cut(s) 1827, 4310
BsaWI WCCGGW 3 cut(s) 1452, 1954, 4199
BsaXI ACNNNNNCTCC 6 cut(s) 3360, 3390, 4073, 4103, 4407, 4437
Bse118I RCCGGY 1 cut(s) 1452
Bse21I CCTNAGG 1 cut(s) 3020
Bse3DI GCAATG 2 cut(s) 2507, 2688
Bse8I GATNNNNATC 3 cut(s) 1429, 2521, 4387
BseBI CCWGG 6 cut(s) 1280, 1388, 2397, 2796, 3441, 4332
BseCI ATCGAT 1 cut(s) 2001
BseJI GATNNNNATC 3 cut(s) 1429, 2521, 4387
BseMI GCAATG 2 cut(s) 2507, 2688
BseRI GAGGAG 3 cut(s) 2664, 3381, 4328
BseSI GKGCMC 1 cut(s) 1744
BseYI CCCAGC 4 cut(s) 659, 881, 2831, 3929
BsgI GTGCAG 1 cut(s) 3899
Bsh1236I CGCG 1 cut(s) 3918
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 6 cut(s) 605, 878, 1044, 1122, 1925, 2794
BshNI GGYRCC 1 cut(s) 2910
BshTI ACCGGT 1 cut(s) 1452
BshVI ATCGAT 1 cut(s) 2001
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 5 cut(s) 472, 1497, 1729, 1744, 3599
BsiHKCI CYCGRG 2 cut(s) 133, 628
BsiSI CCGG 7 cut(s) 1453, 1955, 2907, 3126, 3285, 3517, 4200
BslFI GGGAC 6 cut(s) 220, 847, 1296, 2089, 4245, 4416
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 6 cut(s) 220, 847, 1296, 2089, 4245, 4416
BsmI GAATGC 1 cut(s) 1664
BsnI GGCC 6 cut(s) 605, 878, 1044, 1122, 1925, 2794
Bso31I GGTCTC 2 cut(s) 1827, 4310
BsoBI CYCGRG 2 cut(s) 133, 628
Bsp1286I GDGCHC 5 cut(s) 472, 1497, 1729, 1744, 3599
Bsp1407I TGTACA 1 cut(s) 1171
Bsp1720I GCTNAGC 1 cut(s) 1785
Bsp19I CCATGG 1 cut(s) 3816
Bsp68I TCGCGA 1 cut(s) 3918
BspANI GGCC 6 cut(s) 605, 878, 1044, 1122, 1925, 2794
BspDI ATCGAT 1 cut(s) 2001
BspFNI CGCG 1 cut(s) 3918
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 7 cut(s) 80, 672, 1284, 2912, 3026, 3438, 4404
BspMI ACCTGC 2 cut(s) 1222, 1956
BspOI GCTAGC 1 cut(s) 1965
BspPI GGATC 4 cut(s) 404, 665, 678, 2807
BspQI GCTCTTC 3 cut(s) 477, 2337, 2463
BspT107I GGYRCC 1 cut(s) 2910
BspTNI GGTCTC 2 cut(s) 1827, 4310
BsrDI GCAATG 2 cut(s) 2507, 2688
BsrFI RCCGGY 1 cut(s) 1452
BsrGI TGTACA 1 cut(s) 1171
BssAI RCCGGY 1 cut(s) 1452
BssNI GRCGYC 1 cut(s) 2820
BssT1I CCWWGG 4 cut(s) 238, 3741, 3760, 3816
Bst2UI CCWGG 6 cut(s) 1280, 1388, 2397, 2796, 3441, 4332
Bst6I CTCTTC 8 cut(s) 477, 862, 1872, 2091, 2337, 2463, 3356, 4199
BstACI GRCGYC 1 cut(s) 2820
BstAUI TGTACA 1 cut(s) 1171
BstBAI YACGTR 3 cut(s) 204, 1289, 1939
BstC8I GCNNGC 5 cut(s) 651, 861, 1963, 2721, 3446
BstDSI CCRYGG 3 cut(s) 734, 3816, 4263
BstEII GGTNACC 1 cut(s) 1369
BstENI CCTNNNNNAGG 1 cut(s) 3606
BstFNI CGCG 1 cut(s) 3918
BstH2I RGCGCY 2 cut(s) 52, 2331
BstHHI GCGC 4 cut(s) 51, 102, 2330, 3872
BstMCI CGRYCG 1 cut(s) 304
BstNI CCWGG 6 cut(s) 1280, 1388, 2397, 2796, 3441, 4332
BstNSI RCATGY 5 cut(s) 1024, 1320, 1587, 1771, 2719
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 9 cut(s) 1278, 1386, 2395, 2794, 2906, 3124, 3284, 3439, 4330
BstSFI CTRYAG 1 cut(s) 1677
BstSLI GKGCMC 1 cut(s) 1744
BstUI CGCG 1 cut(s) 3918
BstV2I GAAGAC 4 cut(s) 746, 2491, 4377, 4429
BstX2I RGATCY 4 cut(s) 169, 670, 1110, 2799
BstXI CCANNNNNNTGG 2 cut(s) 2145, 3480
BstYI RGATCY 4 cut(s) 169, 670, 1110, 2799
Bsu15I ATCGAT 1 cut(s) 2001
Bsu36I CCTNAGG 1 cut(s) 3020
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 6 cut(s) 605, 878, 1044, 1122, 1925, 2794
BsuTUI ATCGAT 1 cut(s) 2001
BtgI CCRYGG 3 cut(s) 734, 3816, 4263
BtgZI GCGATG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 2151
BtsI GCAGTG 2 cut(s) 2277, 3692
BtuMI TCGCGA 1 cut(s) 3918
BveI ACCTGC 2 cut(s) 1222, 1956
Cac8I GCNNGC 5 cut(s) 651, 861, 1963, 2721, 3446
CaiI CAGNNNCTG 1 cut(s) 4412
CciI TCATGA 1 cut(s) 915
CfoI GCGC 4 cut(s) 51, 102, 2330, 3872
Cfr10I RCCGGY 1 cut(s) 1452
Cfr13I GGNCC 9 cut(s) 604, 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
ClaI ATCGAT 1 cut(s) 2001
CseI GACGC 2 cut(s) 1662, 2809
Csp6I GTAC 8 cut(s) 263, 1172, 2911, 3835, 3937, 4246, 4300, 4363
CspAI ACCGGT 1 cut(s) 1452
CspCI CAANNNNNGTGG 2 cut(s) 1835, 1870
CviQI GTAC 8 cut(s) 263, 1172, 2911, 3835, 3937, 4246, 4300, 4363
DraI TTTAAA 1 cut(s) 4387
DrdI GACNNNNNNGTC 1 cut(s) 3396
DseDI GACNNNNNNGTC 1 cut(s) 3396
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 8 cut(s) 477, 862, 1872, 2091, 2337, 2463, 3356, 4199
EarI CTCTTC 8 cut(s) 477, 862, 1872, 2091, 2337, 2463, 3356, 4199
EciI GGCGGA 1 cut(s) 1418
Ecl136II GAGCTC 2 cut(s) 470, 1495
Eco130I CCWWGG 4 cut(s) 238, 3741, 3760, 3816
Eco147I AGGCCT 2 cut(s) 1122, 2794
Eco24I GRGCYC 2 cut(s) 472, 1497
Eco31I GGTCTC 2 cut(s) 1827, 4310
Eco32I GATATC 3 cut(s) 1035, 1246, 2968
Eco47I GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
Eco53kI GAGCTC 2 cut(s) 470, 1495
Eco57I CTGAAG 1 cut(s) 2442
Eco72I CACGTG 1 cut(s) 1289
Eco81I CCTNAGG 1 cut(s) 3020
Eco88I CYCGRG 2 cut(s) 133, 628
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 2 cut(s) 470, 1495
EcoNI CCTNNNNNAGG 1 cut(s) 3606
EcoO109I RGGNCCY 1 cut(s) 617
EcoO65I GGTNACC 1 cut(s) 1369
EcoRII CCWGG 6 cut(s) 1278, 1386, 2395, 2794, 3439, 4330
EcoRV GATATC 3 cut(s) 1035, 1246, 2968
EcoT14I CCWWGG 4 cut(s) 238, 3741, 3760, 3816
EcoT38I GRGCYC 2 cut(s) 472, 1497
ErhI CCWWGG 4 cut(s) 238, 3741, 3760, 3816
Esp3I CGTCTC 1 cut(s) 347
FalI AAGNNNNNCTT 4 cut(s) 894, 926, 1521, 1553
FaqI GGGAC 6 cut(s) 220, 847, 1296, 2089, 4245, 4416
FauI CCCGC 1 cut(s) 2066
FauNDI CATATG 3 cut(s) 992, 2179, 3985
FbaI TGATCA 1 cut(s) 2055
FblI GTMKAC 5 cut(s) 276, 379, 906, 1275, 1676
FriOI GRGCYC 2 cut(s) 472, 1497
FspI TGCGCA 1 cut(s) 3871
GlaI GCGC 4 cut(s) 50, 101, 2329, 3871
GsaI CCCAGC 4 cut(s) 663, 885, 2835, 3933
GsuI CTGGAG 4 cut(s) 2614, 2895, 3588, 4434
HaeII RGCGCY 2 cut(s) 52, 2331
HaeIII GGCC 6 cut(s) 605, 878, 1044, 1122, 1925, 2794
HapII CCGG 7 cut(s) 1453, 1955, 2907, 3126, 3285, 3517, 4200
HgaI GACGC 2 cut(s) 1662, 2809
HhaI GCGC 4 cut(s) 51, 102, 2330, 3872
Hin1I GRCGYC 1 cut(s) 2820
Hin6I GCGC 4 cut(s) 49, 100, 2328, 3870
HinP1I GCGC 4 cut(s) 49, 100, 2328, 3870
HincII GTYRAC 4 cut(s) 3040, 3144, 3165, 3862
HindII GTYRAC 4 cut(s) 3040, 3144, 3165, 3862
HindIII AAGCTT 2 cut(s) 676, 3576
HinfI GANTC 9 cut(s) 509, 835, 1414, 1888, 1907, 2077, 2459, 2996, 4428
HpaI GTTAAC 1 cut(s) 3165
HpaII CCGG 7 cut(s) 1453, 1955, 2907, 3126, 3285, 3517, 4200
HphI GGTGA 7 cut(s) 709, 1079, 2160, 2276, 2903, 2967, 4045
Hpy99I CGWCG 3 cut(s) 1141, 2020, 3857
HpyCH4IV ACGT 8 cut(s) 203, 279, 356, 1139, 1288, 1938, 2150, 3503
HpySE526I ACGT 8 cut(s) 203, 279, 356, 1139, 1288, 1938, 2150, 3503
Hsp92I GRCGYC 1 cut(s) 2820
HspAI GCGC 4 cut(s) 49, 100, 2328, 3870
KpnI GGTACC 1 cut(s) 2914
Ksp22I TGATCA 1 cut(s) 2055
KspAI GTTAAC 1 cut(s) 3165
LguI GCTCTTC 3 cut(s) 477, 2337, 2463
LweI GCATC 6 cut(s) 234, 1319, 2021, 3097, 3223, 3474
MaeII ACGT 8 cut(s) 203, 279, 356, 1139, 1288, 1938, 2150, 3503
MfeI CAATTG 4 cut(s) 438, 516, 3507, 3885
MflI RGATCY 4 cut(s) 169, 670, 1110, 2799
MhlI GDGCHC 5 cut(s) 472, 1497, 1729, 1744, 3599
MlsI TGGCCA 1 cut(s) 1044
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 3 cut(s) 829, 1408, 2071
MmeI TCCRAC 5 cut(s) 439, 991, 1060, 3835, 4405
Mox20I TGGCCA 1 cut(s) 1044
MroXI GAANNNNTTC 2 cut(s) 368, 3662
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 8 cut(s) 534, 588, 687, 1773, 2277, 2700, 3164, 4386
MslI CAYNNNNRTG 3 cut(s) 2604, 3206, 3254
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 5 cut(s) 63, 881, 2723, 3245, 3749
MspI CCGG 7 cut(s) 1453, 1955, 2907, 3126, 3285, 3517, 4200
MspR9I CCNGG 9 cut(s) 1280, 1388, 2397, 2796, 2908, 3126, 3286, 3441, 4332
MunI CAATTG 4 cut(s) 438, 516, 3507, 3885
Mva1269I GAATGC 1 cut(s) 1664
MvaI CCWGG 6 cut(s) 1280, 1388, 2397, 2796, 3441, 4332
MvnI CGCG 1 cut(s) 3918
NciI CCSGG 3 cut(s) 2908, 3126, 3286
NcoI CCATGG 1 cut(s) 3816
NdeI CATATG 3 cut(s) 992, 2179, 3985
NheI GCTAGC 1 cut(s) 1961
NlaIV GGNNCC 7 cut(s) 80, 672, 1284, 2912, 3026, 3438, 4404
NmuCI GTSAC 6 cut(s) 697, 710, 1435, 2891, 3398, 4293
NruI TCGCGA 1 cut(s) 3918
NsbI TGCGCA 1 cut(s) 3871
NspI RCATGY 5 cut(s) 1024, 1320, 1587, 1771, 2719
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 915
PceI AGGCCT 2 cut(s) 1122, 2794
PciI ACATGT 2 cut(s) 1316, 1767
PciSI GCTCTTC 3 cut(s) 477, 2337, 2463
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 1664
PdmI GAANNNNTTC 2 cut(s) 368, 3662
PfeI GAWTC 6 cut(s) 509, 1888, 1907, 2459, 2996, 4428
PflMI CCANNNNNTGG 2 cut(s) 4236, 4412
PfoI TCCNGGA 2 cut(s) 2395, 3124
PinAI ACCGGT 1 cut(s) 1452
PleI GAGTC 3 cut(s) 829, 1408, 2071
PmaCI CACGTG 1 cut(s) 1289
PmlI CACGTG 1 cut(s) 1289
PpsI GAGTC 3 cut(s) 829, 1408, 2071
Ppu21I YACGTR 3 cut(s) 204, 1289, 1939
PpuMI RGGWCCY 1 cut(s) 617
PscI ACATGT 2 cut(s) 1316, 1767
PsiI TTATAA 1 cut(s) 1655
Psp124BI GAGCTC 2 cut(s) 472, 1497
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 6 cut(s) 1278, 1386, 2395, 2794, 3439, 4330
PspCI CACGTG 1 cut(s) 1289
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 4 cut(s) 659, 881, 2831, 3929
PspGI CCWGG 6 cut(s) 1278, 1386, 2395, 2794, 3439, 4330
PspN4I GGNNCC 7 cut(s) 80, 672, 1284, 2912, 3026, 3438, 4404
PspPI GGNCC 9 cut(s) 604, 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
PspPPI RGGWCCY 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 4412
PsuI RGATCY 4 cut(s) 169, 670, 1110, 2799
PvuII CAGCTG 3 cut(s) 2723, 3245, 3749
RruI TCGCGA 1 cut(s) 3918
RsaI GTAC 8 cut(s) 264, 1173, 2912, 3836, 3938, 4247, 4301, 4364
RsaNI GTAC 8 cut(s) 263, 1172, 2911, 3835, 3937, 4246, 4300, 4363
RseI CAYNNNNRTG 3 cut(s) 2604, 3206, 3254
SacI GAGCTC 2 cut(s) 472, 1497
SapI GCTCTTC 3 cut(s) 477, 2337, 2463
SaqAI TTAA 8 cut(s) 534, 588, 687, 1773, 2277, 2700, 3164, 4386
Sau96I GGNCC 9 cut(s) 604, 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
SchI GAGTC 3 cut(s) 829, 1408, 2071
ScrFI CCNGG 9 cut(s) 1280, 1388, 2397, 2796, 2908, 3126, 3286, 3441, 4332
SduI GDGCHC 5 cut(s) 472, 1497, 1729, 1744, 3599
SfaNI GCATC 6 cut(s) 234, 1319, 2021, 3097, 3223, 3474
SfcI CTRYAG 1 cut(s) 1677
Sfr274I CTCGAG 1 cut(s) 133
SinI GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
SlaI CTCGAG 1 cut(s) 133
SmiI ATTTAAAT 1 cut(s) 4387
SmiMI CAYNNNNRTG 3 cut(s) 2604, 3206, 3254
SmlI CTYRAG 5 cut(s) 133, 255, 941, 1375, 3488
SmoI CTYRAG 5 cut(s) 133, 255, 941, 1375, 3488
SseBI AGGCCT 2 cut(s) 1122, 2794
SspI AATATT 1 cut(s) 3425
SstI GAGCTC 2 cut(s) 472, 1497
StuI AGGCCT 2 cut(s) 1122, 2794
StyD4I CCNGG 9 cut(s) 1278, 1386, 2395, 2794, 2906, 3124, 3284, 3439, 4330
StyI CCWWGG 4 cut(s) 238, 3741, 3760, 3816
SwaI ATTTAAAT 1 cut(s) 4387
TaiI ACGT 8 cut(s) 206, 282, 359, 1142, 1291, 1941, 2153, 3506
TaqII GACCGA 2 cut(s) 1423, 1709
TatI WGTACW 4 cut(s) 262, 1171, 3936, 4299
TauI GCSGC 4 cut(s) 60, 881, 1664, 4169
TfiI GAWTC 6 cut(s) 509, 1888, 1907, 2459, 2996, 4428
Tru1I TTAA 8 cut(s) 534, 588, 687, 1773, 2277, 2700, 3164, 4386
Tru9I TTAA 8 cut(s) 534, 588, 687, 1773, 2277, 2700, 3164, 4386
TseFI GTSAC 6 cut(s) 697, 710, 1435, 2891, 3398, 4293
Tsp45I GTSAC 6 cut(s) 697, 710, 1435, 2891, 3398, 4293
TspGWI ACGGA 1 cut(s) 751
Van91I CCANNNNNTGG 2 cut(s) 4236, 4412
VneI GTGCAC 1 cut(s) 1740
VpaK11BI GGWCC 8 cut(s) 617, 1082, 1283, 1692, 2049, 3389, 3514, 4403
XagI CCTNNNNNAGG 1 cut(s) 3606
XapI RAATTY 8 cut(s) 310, 1442, 2133, 2901, 2940, 3452, 4058, 4393
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 5 cut(s) 1024, 1320, 1587, 1771, 2719
XcmI CCANNNNNNNNNTGG 2 cut(s) 2653, 4356
XhoI CTCGAG 1 cut(s) 133
XmiI GTMKAC 5 cut(s) 276, 379, 906, 1275, 1676
XmnI GAANNNNTTC 2 cut(s) 368, 3662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.