pycom09g18560

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005927
Physical Location & Seq
Forward (+)
19360836 .. 19361384
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGAGACAAATGGCAAAACACGAAGAACTCCCAATCCCAATTCACAACACCCTCGACGCCGTCTACGGCGACTCCTCCCAACTCGACGAGGCCCAGCTCCGATTCCACCGGCTGAAGTCCAAGTTCCAGCAGGTCGGTCACCCTCCCCAACTCTTCTCTCGCTCTTCAGGGAGAGTAAACTTGATCGGAGAGCACATAGACTACGAGGGCTATTCAGTGCTGCCGATGGCCATACGACAGGACCCCATTGTCGCAATCCGGAAGCACGACGACGGAGATGCCGAGAAAGTTCTCAGAATCGCCAACGTCAACGACAAGTACCAAATTTGTACTTATGCCGCCGACCCGACCCAGGAAATTGACTTGAAGAATCACAAATAG

Protein Analysis

127

Amino Acids

14.43

Weight (kDa)

6.36

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 43 - 88 3.8e-18 Galactokinase galactose-binding signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 63
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 2 cut(s) 134, 150
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 320, 331
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 351
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
Alw21I GWGCWC 1 cut(s) 195
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 2 cut(s) 90, 228
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 91, 241
AsuHPI GGTGA 1 cut(s) 131
AvaII GGWCC 1 cut(s) 241
BalI TGGCCA 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 207
BccI CCATC 1 cut(s) 220
BceAI ACGGC 2 cut(s) 44, 82
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 2 cut(s) 221, 339
BlsI GCNGC 2 cut(s) 222, 340
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 91, 241
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 1 cut(s) 268
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMII CTCAG 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 2 cut(s) 92, 230
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 2 cut(s) 109, 259
BslI CCNNNNNNNGG 1 cut(s) 352
BsnI GGCC 2 cut(s) 92, 230
Bsp1286I GDGCHC 1 cut(s) 195
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 1 cut(s) 339
BspANI GGCC 2 cut(s) 92, 230
BspCNI CTCAG 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 243
BspMI ACCTGC 1 cut(s) 121
BspQI GCTCTTC 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 183
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 353
Bst6I CTCTTC 2 cut(s) 158, 169
BstACI GRCGYC 1 cut(s) 57
BstDEI CTNAG 1 cut(s) 293
BstEII GGTNACC 1 cut(s) 137
BstKTI GATC 1 cut(s) 186
BstMBI GATC 1 cut(s) 183
BstNI CCWGG 1 cut(s) 353
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 207
BsuRI GGCC 2 cut(s) 92, 230
BtsIMutI CAGTG 1 cut(s) 222
BveI ACCTGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 91, 241
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 319, 330
CviJI RGCY 5 cut(s) 92, 97, 112, 210, 230
CviKI_1 RGCY 5 cut(s) 92, 97, 112, 210, 230
CviQI GTAC 2 cut(s) 319, 330
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 158, 169
EarI CTCTTC 2 cut(s) 158, 169
Eco47I GGWCC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 134, 150
Eco91I GGTNACC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 351
FaiI YATR 3 cut(s) 197, 233, 336
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 221, 339
Fsp4HI GCNGC 2 cut(s) 221, 339
GluI GCNGC 2 cut(s) 221, 339
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 2 cut(s) 92, 230
HapII CCGG 2 cut(s) 109, 259
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 71, 102, 297, 370
HpaII CCGG 2 cut(s) 109, 259
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 64, 178, 310
Hpy188I TCNGA 3 cut(s) 101, 188, 296
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 3 cut(s) 64, 178, 310
Hpy99I CGWCG 4 cut(s) 59, 89, 272, 275
HpyCH4IV ACGT 1 cut(s) 306
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 57
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 1 cut(s) 183
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 9 cut(s) 107, 116, 122, 140, 153, 224, 272, 338, 365
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 1 cut(s) 268
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 137
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 4 cut(s) 35, 145, 156, 379
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 3 cut(s) 39, 324, 357
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 5 cut(s) 62, 82, 85, 153, 199
Mox20I TGGCCA 1 cut(s) 230
MroI TCCGGA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 230
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 2 cut(s) 109, 259
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
NdeII GATC 1 cut(s) 183
NlaIV GGNNCC 1 cut(s) 243
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 3 cut(s) 102, 297, 370
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 222, 340
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 351
PspEI GGTNACC 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 243
PspPI GGNCC 2 cut(s) 91, 241
PspPPI RGGWCCY 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 2 cut(s) 320, 331
RsaNI GTAC 2 cut(s) 319, 330
SapI GCTCTTC 1 cut(s) 169
SatI GCNGC 2 cut(s) 221, 339
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 91, 241
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 195
SetI ASST 3 cut(s) 99, 135, 309
SfaNI GCATC 1 cut(s) 268
SinI GGWCC 1 cut(s) 241
Sse9I AATT 3 cut(s) 39, 324, 357
SsiI CCGC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 351
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 54, 84
TaqII GACCGA 1 cut(s) 125
TasI AATT 3 cut(s) 39, 324, 357
TatI WGTACW 1 cut(s) 329
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 3 cut(s) 102, 297, 370
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 1 cut(s) 222
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.