pycom10g01220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002389
Physical Location & Seq
Reverse (-)
1277653 .. 1278672
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g01220.2

Sequence Viewer

Length: 699 bp
ATGAAGGTGCAGATATGTGAAGGGCCTAACATGAAGGTATATGAAGGGGCTAACACAAAGGTGCAGATATGTGAAGGTAGTACCCCTGAACATGAGGCCGAGGGTAGTAATGATTGTGATAATGGTCAGAAGCTTGGTAGTCGGTGCAGTGAGGTTCATAAGGATGAATTCAATTCCTCCGATGACGTCGCCAAATCTGTGGAAACCTCTCTAGTTACTACTTGCGACCCTTTAAAAATCTCAGCAATCATTTGTTATGTTGCTGCACAGCCTATTATTGATCCAATCTGGAGGGGAAGTCTCAGCATATTCAACAAAGACTTCAACATTGTTAGTGGACTTGTAGCCCATCTTTCTAGCTTAGCATGTCCTAAAGTACGTGAGGAGGCAGAATCGCTACCGTTGCTGCTTTTTCCAGAACTGGTTAATAGAACAGATGTGTGGCCCAAAGCTTTCGAGAAGTGTGGACCTAACGATCAGAGTATTGCTCTTTATTTCTTTCCTGATAACGAAAGAGATGAGAAGGATTTTGATACCCTGGTGGTTAGCATGATCCAGGATGATTTAGCTATGGGAGCTGTGCTGGAGGATGCTGAGCTCTTAGTTTTCACGTCAGTTATACTGCCAGAGCAGTATCGAAGATTTCAGACAAAGTTTTATTTGTGGGGAGTATTTAGGGCAAAGCAATTTCGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

26.05

Weight (kDa)

4.69

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AclWI GGATC 2 cut(s) 275, 547
AcsI RAATTY 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 82, 378
AgsI TTSAA 3 cut(s) 172, 313, 325
AjiI CACGTC 1 cut(s) 612
AjnI CCWGG 2 cut(s) 537, 555
AleI CACNNNNGTG 1 cut(s) 59
AluBI AGCT 6 cut(s) 133, 360, 452, 569, 578, 598
AluI AGCT 6 cut(s) 133, 360, 452, 569, 578, 598
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 2 cut(s) 275, 547
AoxI GGCC 3 cut(s) 23, 96, 443
ApeKI GCWGC 2 cut(s) 263, 406
ApoI RAATTY 1 cut(s) 167
AspS9I GGNCC 3 cut(s) 23, 444, 467
AvaII GGWCC 1 cut(s) 467
BaeI ACNNNNGTAYC 2 cut(s) 525, 558
BanII GRGCYC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 2 cut(s) 250, 393
BccI CCATC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 539, 557
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 2 cut(s) 212, 357
BisI GCNGC 2 cut(s) 264, 407
BlpI GCTNAGC 2 cut(s) 361, 594
BlsI GCNGC 2 cut(s) 265, 408
Bme1390I CCNGG 2 cut(s) 539, 557
Bme18I GGWCC 1 cut(s) 467
BmgBI CACGTC 1 cut(s) 612
BmgT120I GGNCC 3 cut(s) 23, 444, 467
BmrFI CCNGG 2 cut(s) 539, 557
BmsI GCATC 1 cut(s) 580
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpmI CTGGAG 2 cut(s) 310, 605
Bpu1102I GCTNAGC 2 cut(s) 361, 594
BsaAI YACGTR 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 99, 537
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 539, 557
BseDI CCNNGG 2 cut(s) 99, 537
BseGI GGATG 3 cut(s) 169, 565, 595
BseMII CTCAG 3 cut(s) 255, 316, 585
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 250, 393
BsgI GTGCAG 4 cut(s) 29, 83, 166, 249
BshFI GGCC 3 cut(s) 25, 98, 445
BsiHKAI GWGCWC 1 cut(s) 600
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 3 cut(s) 25, 98, 445
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 3 cut(s) 280, 475, 552
Bsp1720I GCTNAGC 2 cut(s) 361, 594
BspANI GGCC 3 cut(s) 25, 98, 445
BspCNI CTCAG 3 cut(s) 254, 315, 586
BspPI GGATC 2 cut(s) 275, 547
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 99, 537
BssMI GATC 3 cut(s) 280, 475, 552
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 539, 557
Bst4CI ACNGT 2 cut(s) 402, 696
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 380
BstDEI CTNAG 5 cut(s) 241, 302, 361, 594, 601
BstF5I GGATG 3 cut(s) 169, 565, 595
BstKTI GATC 3 cut(s) 283, 478, 555
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 3 cut(s) 280, 475, 552
BstMWI GCNNNNNNNGC 2 cut(s) 403, 575
BstNI CCWGG 2 cut(s) 539, 557
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 537, 555
BstV1I GCAGC 2 cut(s) 250, 393
BstXI CCANNNNNNTGG 1 cut(s) 199
BsuRI GGCC 3 cut(s) 25, 98, 445
BtrI CACGTC 1 cut(s) 612
BtsCI GGATG 3 cut(s) 169, 565, 595
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 23, 444, 467
Csp6I GTAC 2 cut(s) 81, 377
CviAII CATG 4 cut(s) 31, 92, 366, 550
CviQI GTAC 2 cut(s) 81, 377
DdeI CTNAG 5 cut(s) 241, 302, 361, 594, 601
DpnI GATC 3 cut(s) 282, 477, 554
DpnII GATC 3 cut(s) 280, 475, 552
DraI TTTAAA 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 537, 555
EcoT38I GRGCYC 1 cut(s) 600
FaeI CATG 4 cut(s) 34, 95, 369, 553
FatI CATG 4 cut(s) 30, 91, 365, 549
Fnu4HI GCNGC 2 cut(s) 264, 407
FokI GGATG 3 cut(s) 176, 572, 602
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 2 cut(s) 264, 407
FspBI CTAG 2 cut(s) 212, 357
GluI GCNGC 2 cut(s) 264, 407
GsuI CTGGAG 2 cut(s) 310, 605
HaeIII GGCC 3 cut(s) 25, 98, 445
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 4 cut(s) 34, 95, 369, 553
HindIII AAGCTT 2 cut(s) 131, 450
HinfI GANTC 1 cut(s) 392
Hpy166II GTNNAC 2 cut(s) 338, 467
Hpy188I TCNGA 4 cut(s) 129, 181, 480, 648
Hpy188III TCNNGA 4 cut(s) 289, 416, 457, 503
Hpy8I GTNNAC 2 cut(s) 338, 467
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 5 cut(s) 14, 28, 38, 68, 517
HpyCH4III ACNGT 2 cut(s) 402, 696
HpyCH4IV ACGT 3 cut(s) 186, 379, 611
HpyCH4V TGCA 4 cut(s) 10, 64, 147, 266
HpyF10VI GCNNNNNNNGC 2 cut(s) 403, 575
HpyF3I CTNAG 5 cut(s) 241, 302, 361, 594, 601
HpySE526I ACGT 3 cut(s) 186, 379, 611
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 4 cut(s) 34, 95, 369, 553
Kzo9I GATC 3 cut(s) 280, 475, 552
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 2 cut(s) 250, 393
LweI GCATC 1 cut(s) 580
MaeI CTAG 2 cut(s) 212, 357
MaeII ACGT 3 cut(s) 186, 379, 611
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 282, 477, 554
MboI GATC 3 cut(s) 280, 475, 552
MboII GAAGA 1 cut(s) 651
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 3 cut(s) 167, 172, 686
MnlI CCTC 9 cut(s) 88, 94, 145, 187, 217, 285, 376, 379, 580
MseI TTAA 2 cut(s) 233, 426
MslI CAYNNNNRTG 2 cut(s) 59, 162
MspR9I CCNGG 2 cut(s) 539, 557
MvaI CCWGG 2 cut(s) 539, 557
MwoI GCNNNNNNNGC 2 cut(s) 403, 575
NdeII GATC 3 cut(s) 280, 475, 552
NlaIII CATG 4 cut(s) 34, 95, 369, 553
NmeAIII GCCGAG 1 cut(s) 124
NspI RCATGY 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 59
PfeI GAWTC 1 cut(s) 392
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 2 cut(s) 265, 408
Ppu21I YACGTR 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 2 cut(s) 537, 555
PspGI CCWGG 2 cut(s) 537, 555
PspPI GGNCC 3 cut(s) 23, 444, 467
RsaI GTAC 2 cut(s) 82, 378
RsaNI GTAC 2 cut(s) 81, 377
RseI CAYNNNNRTG 2 cut(s) 59, 162
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 233, 426
SatI GCNGC 2 cut(s) 264, 407
Sau3AI GATC 3 cut(s) 280, 475, 552
Sau96I GGNCC 3 cut(s) 23, 444, 467
ScrFI CCNGG 2 cut(s) 539, 557
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 1 cut(s) 580
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 59, 162
Sse9I AATT 3 cut(s) 167, 172, 686
SspMI CTAG 2 cut(s) 212, 357
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 2 cut(s) 537, 555
TaaI ACNGT 2 cut(s) 402, 696
TaiI ACGT 3 cut(s) 189, 382, 614
TaqI TCGA 3 cut(s) 456, 637, 691
TasI AATT 3 cut(s) 167, 172, 686
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 2 cut(s) 233, 426
Tru9I TTAA 2 cut(s) 233, 426
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 263, 406
TspDTI ATGAA 5 cut(s) 17, 47, 57, 146, 180
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 369
XspI CTAG 2 cut(s) 212, 357
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.