pycom10g01910

Abscisic stress-ripening protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004221
Physical Location & Seq
Forward (+)
2012565 .. 2013611
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGTCTGAAGAGAAGCACCACCACGGTCACCACCACCACCACCACCACAAGGATGAAGACAAACCCTCATACTACCCTCAGTCTGGCTATAGCGAGACCACCGTGGCATATGGCGCCACCAACACTCTTGAACCTGAAATTGATTACAAGAAAGAGGAGAAGCACCACAAGCATCTCGAGCACCTCGGGGAGGCCGGTGTTGTTGCTGCCGGCGCTTATGCCTTGCATGAGAAACACAAGGCAAAGAAAGACCCAGAGCATGCCCACAAGCACAAGATAGAAGAAGAGATTGCTGCAGCAGCTGCAGTTGGTGCCGGTGGATTTGCCTTCCATGAGCATCATCAGAAGAAAGAGACAAAGAAAGAAGAGGAAGAGGCTTATGGAAAGAAGCACCACCACCTCTTCTAG

Protein Analysis

136

Amino Acids

15.46

Weight (kDa)

6.48

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABA_WDS PF02496 48 - 125 2.1e-36 ABA/WDS induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 113, 311
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 49, 83, 190
AgsI TTSAA 1 cut(s) 131
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 89, 347
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 2 cut(s) 176, 185
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 5 cut(s) 206, 293, 296, 299, 302
AspLEI GCGC 2 cut(s) 116, 215
AsuHPI GGTGA 1 cut(s) 20
AvaI CYCGRG 2 cut(s) 176, 185
BanI GGYRCC 2 cut(s) 113, 311
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 5 cut(s) 193, 280, 289, 308, 311
BcoDI GTCTC 2 cut(s) 89, 347
BfaI CTAG 1 cut(s) 406
BfmI CTRYAG 3 cut(s) 88, 294, 303
BfoI RGCGCY 2 cut(s) 117, 216
BisI GCNGC 5 cut(s) 207, 294, 297, 300, 303
BlsI GCNGC 5 cut(s) 208, 295, 298, 301, 304
BmeT110I CYCGRG 2 cut(s) 176, 185
BmiI GGNNCC 2 cut(s) 115, 313
BmsI GCATC 2 cut(s) 181, 346
BpiI GAAGAC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 22, 102, 184
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 83, 190
Bse118I RCCGGY 3 cut(s) 194, 209, 314
BseDI CCNNGG 3 cut(s) 22, 102, 184
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 3 cut(s) 49, 83, 190
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 5 cut(s) 193, 280, 289, 308, 311
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 2 cut(s) 113, 311
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 2 cut(s) 176, 185
BsiSI CCGG 3 cut(s) 195, 210, 315
BslI CCNNNNNNNGG 3 cut(s) 49, 83, 190
BsmAI GTCTC 2 cut(s) 89, 347
BsnI GGCC 1 cut(s) 194
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 176, 185
Bsp1286I GDGCHC 1 cut(s) 183
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 115, 313
BspMAI CTGCAG 2 cut(s) 298, 307
BspT107I GGYRCC 2 cut(s) 113, 311
BspTNI GGTCTC 1 cut(s) 89
BsrFI RCCGGY 3 cut(s) 194, 209, 314
BssAI RCCGGY 3 cut(s) 194, 209, 314
BssECI CCNNGG 3 cut(s) 22, 102, 184
BssNI GRCGYC 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 26, 103
Bst6I CTCTTC 4 cut(s) 3, 279, 360, 366
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 2 cut(s) 302, 311
BstC8I GCNNGC 2 cut(s) 211, 261
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 2 cut(s) 22, 102
BstEII GGTNACC 1 cut(s) 26
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 58
BstH2I RGCGCY 2 cut(s) 117, 216
BstHHI GCGC 2 cut(s) 116, 215
BstMAI GTCTC 2 cut(s) 89, 347
BstMWI GCNNNNNNNGC 7 cut(s) 113, 169, 178, 212, 299, 302, 311
BstNSI RCATGY 1 cut(s) 263
BstPI GGTNACC 1 cut(s) 26
BstSFI CTRYAG 3 cut(s) 88, 294, 303
BstV1I GCAGC 5 cut(s) 193, 280, 289, 308, 311
BstV2I GAAGAC 1 cut(s) 63
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 2 cut(s) 22, 102
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 211, 261
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 2 cut(s) 116, 215
Cfr10I RCCGGY 3 cut(s) 194, 209, 314
CviAII CATG 3 cut(s) 227, 260, 332
CviJI RGCY 4 cut(s) 87, 194, 302, 377
CviKI_1 RGCY 4 cut(s) 87, 194, 302, 377
DdeI CTNAG 1 cut(s) 78
DinI GGCGCC 1 cut(s) 115
Eam1104I CTCTTC 4 cut(s) 3, 279, 360, 366
EarI CTCTTC 4 cut(s) 3, 279, 360, 366
Eco31I GGTCTC 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 2 cut(s) 176, 185
Eco91I GGTNACC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 26
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
FaeI CATG 3 cut(s) 230, 263, 335
FaiI YATR 9 cut(s) 70, 90, 109, 111, 219, 228, 261, 333, 381
FatI CATG 3 cut(s) 226, 259, 331
FauNDI CATATG 1 cut(s) 109
Fnu4HI GCNGC 5 cut(s) 207, 294, 297, 300, 303
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 5 cut(s) 207, 294, 297, 300, 303
FspBI CTAG 1 cut(s) 406
GlaI GCGC 2 cut(s) 115, 214
GluI GCNGC 5 cut(s) 207, 294, 297, 300, 303
HaeII RGCGCY 2 cut(s) 117, 216
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 195, 210, 315
HhaI GCGC 2 cut(s) 116, 215
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 3 cut(s) 230, 263, 335
Hin6I GCGC 2 cut(s) 114, 213
HinP1I GCGC 2 cut(s) 114, 213
HpaII CCGG 3 cut(s) 195, 210, 315
HphI GGTGA 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 7, 345
Hpy188III TCNNGA 2 cut(s) 128, 176
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 2 cut(s) 26, 103
HpyCH4V TGCA 3 cut(s) 226, 296, 305
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 169, 178, 212, 299, 302, 311
HpyF3I CTNAG 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 3 cut(s) 230, 263, 335
HspAI GCGC 2 cut(s) 114, 213
KasI GGCGCC 1 cut(s) 113
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
LpnPI CCDG 6 cut(s) 69, 147, 208, 223, 267, 328
Lsp1109I GCAGC 5 cut(s) 193, 280, 289, 308, 311
LweI GCATC 2 cut(s) 181, 346
MaeI CTAG 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 26
MboII GAAGA 8 cut(s) 20, 68, 293, 296, 358, 377, 383, 394
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 1 cut(s) 138
Mly113I GGCGCC 1 cut(s) 114
MnlI CCTC 7 cut(s) 76, 87, 148, 184, 194, 361, 367
MroNI GCCGGC 1 cut(s) 209
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 195, 210, 315
MwoI GCNNNNNNNGC 7 cut(s) 113, 169, 178, 212, 299, 302, 311
NaeI GCCGGC 1 cut(s) 211
NarI GGCGCC 1 cut(s) 114
NdeI CATATG 1 cut(s) 109
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 3 cut(s) 230, 263, 335
NlaIV GGNNCC 2 cut(s) 115, 313
NmuCI GTSAC 1 cut(s) 26
NspI RCATGY 1 cut(s) 263
PaeI GCATGC 1 cut(s) 263
PaeR7I CTCGAG 1 cut(s) 176
PdiI GCCGGC 1 cut(s) 211
PkrI GCNGC 5 cut(s) 208, 295, 298, 301, 304
PluTI GGCGCC 1 cut(s) 117
PspEI GGTNACC 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 115, 313
PstI CTGCAG 2 cut(s) 298, 307
PstNI CAGNNNCTG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 5 cut(s) 207, 294, 297, 300, 303
SduI GDGCHC 1 cut(s) 183
SetI ASST 4 cut(s) 136, 186, 304, 402
SfaNI GCATC 2 cut(s) 181, 346
SfcI CTRYAG 3 cut(s) 88, 294, 303
SfoI GGCGCC 1 cut(s) 115
Sfr274I CTCGAG 1 cut(s) 176
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SphI GCATGC 1 cut(s) 263
Sse9I AATT 1 cut(s) 138
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 26, 103
TaqI TCGA 1 cut(s) 177
TasI AATT 1 cut(s) 138
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 5 cut(s) 206, 293, 296, 299, 302
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 69
XagI CCTNNNNNAGG 1 cut(s) 188
XceI RCATGY 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 176
XspI CTAG 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.