Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

3390673 .. 3398666

Gene Structure 7994 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3210 bp

ATGCCTTCACTAAAACGTCCACTCTCACAACAATCTCTCTCAGTCTCTCTTCAATCTCTCTCAACGCGCTCAAAGAAAGGGCCTCTTCTTCGACACAAAGTTCAAGAGATGATTTCAGAGAAGCCCAGTTGGATTAGACATGAGGGAATGCAAATTTTCTCCATTGACGTTCAACCTGGTGGACTTAGGCTTGCCACTGGTGGGGGTGATCACAAGGTGCGGGTATGGAATATGAAATCCCTTGGCAGGGATTTGGATAATGAAGAATCAGCCCAGAGGCTACTTGCAACTCTCCGTGATCACTTTGGTTCTGTGAATTGTGTTAGGTGGGCTAAACATGGTCAGTTTCTTGCATCAGGATCTGATGACCAAGTGATTCTAATTCATGAGAGAAAGCCTGGTTCAGGAACCACTGAGTTTGGCAGTGGAGAGCCACCTGACCTTGAAAATTGGAAAGTTTCAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTTAATTGGTCTCCAGATGACTTGATGTTGGCCAGTGGAAGTTTAGATAACACTATACACATCTGGAACATGAGCAATGGTATTTGCACTGCTGTTCTGAGGGGTCACTCTAGCCTGGTTAAAGGAGTTACTTGGGATCCAATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACTGTAATTATTTGGCGAACAAGTGACTGGAGCCTTGCTCACAGGACAGACGGCCATTGGCAAAAATCACTTGGATCCACCTTTTTCAGGCGGCTGGGATGGTCCCCTTGTGGCCACTTCATTACTACCACTCACGGATTCCAGAAACCAAGGCATTCTGCACCTGTTCTAGAGAGAGGGGAATGGTCTGCTACATTTGACTTCTTAGGACATAATGCCCCTGTGATCGTGGTGAAGTTTAACCATTCAATGTTTAGGAGGAATATTTCCAATGCTCAGGAAAAAGCTGCACCCATCGGGTGGACTAATGGGGCTTCAAAGACAGGAGAAAAAGAGAAAGAACCACAACCTTATAATGTCATTGCTATTGGGAGTCAGGACAAGACTATAACAGTATGGACTACTGCAAGTCCCCGACCTCTCTTTGTAGCAAAGCATTTCTTTACTCAAAGTGTTGTGGATTTATCCTGGAGCCCTGATGGCTATTCACTTTTTGCATGTTCTTTGGATGGTTCGGTGGCAACTTTCCATTTTGATGTTAAAGAACTTGGTAACCGTTTAAGCGATGCTGAACTGGATGAATTGAAGAGAAACCGTTATGGCGATGTTAGAGGTCGGCAAGCAAATTTAGTAGAAAGCCCAGCTCAGTTATTGTTTGAAGCAGCATCTGCTAAGCAAGCCTCAAGCGAAAAACTGGTTCTGGAAGTTCAGCAAAACGAGACAGTTGTAAAACCTTCTACTGAAGCAACGGTTGCAACAATAACTTCTGCAGACAGCTTAAATAAGGTTTCAATACCTGCCAGAATTTCAAGTCCTGTGAAGCAGAGGGAATATAGACGCCCTGATGGTAGAAAGAGGATTATTCCAGAAGCAGTTGGGGTGCCTCTGCAGCAGGAAAATATATCTGCTGGGGTTCAAACCCCGGCACTTGACTTCCCTTCTGTACGTTCTGATAAAAACAATGATGACAATGGATTAATTGCTGCTGATAGTGGCATCAAAGAAAGTTCTGTTAGGGGAGTAATTGGCAGAAGCACTGAAATAAAGGAAGGACATGGGGTTACTGCTAGAGCTATGATTACCAAGAGCCTTGTTATTGAGAAGGTTCCTGCTTCCACAGGTGGAGATGAAAGCATAGCTGTGGAACAGTCAGGTAATTCGAAGGCGTCTAGTTCAGCAGGTTCTTCATGTTCCACTCTTTCAATAAGGGTGTTTGATAGGAAAGAAGGGGAAGACAATGTACCAGTTTTCTTGGAAGCTCGACCTCGGGAACACGCTGCAAACGACATCGTTGGCTTGGGAAATACATTTATCATGAAAGAAACAGAAATTACTTGCGCAAGAGGGTTACAGACTCTTTGGTCAGATAGGATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGCAAACTTCTGGGCTGTTGGGTGTGAAGATGGATGCATACAGGTTTACACAACATGTGGGAGACGTGCTATGCCAACTATGATGGTAGGATCCGGATCAATATTTATAGATTGTGATGAGCGCTGGAAATTATTTTTGGTGACAAGAAAAGGATCCTTTTATGTATGGGATCTATTCAAGCAGAATTGTCTCCTCCATGACTCATTGGCATCTCTGGTCGCTTCAAACCCAAACCCATCTGCAAAAGATTCCAGGCGTGATCAAAGTTATATCAGCAAAGCTATCAATCGATCTGGTTCTCCTCTTGTTGTTCTTGCCACTCGCCATGCCTTCCTCTTTGACATGGGCCTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTCCTGGGTCAAATTTTGCAAGCTCCTGGCATTCAGGTTCAACTCAGGGTGGTGAGCTGGCTGCTTTGCAGATTGATGTTAGGAAGTATGTGGCCAGAAAGCCAGGTTGGAGCAGGGTGACAGACGATGGGGTGCAGACACGTGCTCACTTGGAGGCTCAGTTGGCTTCCTCGCTAGCTTTAAAGTCAGCTAAAGACTATTGCCAATGCCTTCTATCATATATTCGCTTCCTTGCAAGAGAAGCAGATGAGTCTCGTTTACGAGAAGTGTGTGAGAGTTTCCTTGGACCTCCAACTGGGATGGTGGACGATACAACTTTAGATTTAAACAACTCGGCATGGGATCCTTGTGTGCTTGGAATGAGGAAACATAAACTTTTACGAGAAGATATCCTTCCGGCAATGGCTTCAAATAGAAAAGTACAGAGATTGCTTAATGAATTGATCGATCTCATTTCCGAATATGAAAGTGCTGAAACAAATATAGAGAAAAAGATTCAAACTTCGCTGACAGCACATCCACCAGCAGCTGATGAAATGGACTCTGCTCCTTCCAGGACAAACGAATTGGACGTTGTCCCTGCAGCAACAGAGAAAACGAAGTCTGTTCCTGCCTCAACTGACCAAAAGGACTCTTCCCAGTTAGCAACAGATACAGAAAATTCTGCTCCAGTAGCCGAAGACAAAGTTAATCCAGATCCGCCAATGATCAGTCAAGTTAGTGTTGCTGCACAAGATGCAGGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1070

Amino Acids

117.09

Weight (kDa)

6.54

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	47 - 134	3.5e-08	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	47 - 408	3.5e-56	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	51 - 135	3.9e-09	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	51 - 236	1.3e-31	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	54 - 237	1.4e-15	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	59 - 229	3.9e-11	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	91 - 299	1.5e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	93 - 128	1.6e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	102 - 307	5.8e-26	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	104 - 266	4.8e-07	WDR35/TULP4 N-terminal
Beta-prop_WDR36-Utp21_2nd	PF25168	147 - 231	8.9e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	148 - 247	3.2e-17	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	148 - 234	2.8e-13	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	151 - 247	6.9e-09	WDR3 second beta-propeller domain
WD40	PF00400	152 - 189	2.7e-09	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	155 - 320	9.2e-15	WDHD1 first WD40 domain
Beta-prop_Aladin	PF25460	159 - 267	4.4e-08	Aladin seven-bladed propeller
WD40	PF00400	194 - 230	2.8e-06	WD domain, G-beta repeat
Hira	PF07569	713 - 913	2e-46	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1029
AasI	GACNNNNNNGTC	1 cut(s)	2035
Acc16I	TGCGCA	1 cut(s)	2014
Acc36I	ACCTGC	3 cut(s)	1480, 1844, 3191
AccB1I	GGYRCC	1 cut(s)	1555
AccB7I	CCANNNNNTGG	1 cut(s)	975
AccII	CGCG	1 cut(s)	67
AccIII	TCCGGA	1 cut(s)	2171
AciI	CCGC	3 cut(s)	220, 766, 3161
AcoI	YGGCCR	4 cut(s)	528, 727, 787, 2553
AcsI	RAATTY	5 cut(s)	153, 1300, 1479, 2473, 3121
AcuI	CTGAAG	1 cut(s)	1437
AcvI	CACGTG	1 cut(s)	2603
AcyI	GRCGYC	2 cut(s)	1513, 1841
AdeI	CACNNNGTG	1 cut(s)	217
AfaI	GTAC	3 cut(s)	1620, 1917, 2883
AfeI	AGCGCT	1 cut(s)	2201
AfiI	CCNNNNNNNGG	7 cut(s)	201, 246, 247, 404, 762, 975, 2756
AflIII	ACRYGT	2 cut(s)	2132, 2600
AjiI	CACGTC	1 cut(s)	2144
AjnI	CCWGG	9 cut(s)	175, 397, 612, 1142, 2330, 2464, 2486, 2563, 3014
AjuI	GAANNNNNNNTTGG	4 cut(s)	2198, 2230, 2551, 2583
Alw21I	GWGCWC	1 cut(s)	2608
Alw26I	GTCTC	6 cut(s)	49, 513, 1388, 2134, 2273, 2718
AlwNI	CAGNNNCTG	3 cut(s)	362, 1343, 2990
Ama87I	CYCGRG	1 cut(s)	1941
Aor13HI	TCCGGA	1 cut(s)	2171
Aor51HI	AGCGCT	1 cut(s)	2201
AoxI	GGCC	6 cut(s)	80, 528, 727, 787, 2425, 2553
ApeKI	GCWGC	9 cut(s)	962, 1337, 1564, 1658, 1952, 2522, 2987, 3044, 3188
ApoI	RAATTY	5 cut(s)	153, 1300, 1479, 2473, 3121
AseI	ATTAAT	1 cut(s)	1652
AspLEI	GCGC	3 cut(s)	69, 2015, 2202
AspS9I	GGNCC	4 cut(s)	80, 777, 2425, 2747
AsuC2I	CCSGG	1 cut(s)	1598
AsuHPI	GGTGA	5 cut(s)	218, 919, 2230, 2525, 2590
AsuII	TTCGAA	1 cut(s)	1835
AsuNHI	GCTAGC	1 cut(s)	2635
AvaI	CYCGRG	1 cut(s)	1941
AvaII	GGWCC	2 cut(s)	777, 2747
BalI	TGGCCA	3 cut(s)	530, 789, 2555
BamHI	GGATCC	5 cut(s)	634, 749, 2168, 2231, 2803
BanI	GGYRCC	1 cut(s)	1555
BanII	GRGCYC	1 cut(s)	1151
BarI	GAAGNNNNNNTAC	2 cut(s)	1899, 1931
BbrPI	CACGTG	1 cut(s)	2603
BbsI	GAAGAC	2 cut(s)	1914, 3147
Bbv12I	GWGCWC	1 cut(s)	2608
BbvI	GCAGC	9 cut(s)	949, 1349, 1576, 1645, 1939, 2509, 2999, 3056, 3175
BceAI	ACGGC	1 cut(s)	742
BciT130I	CCWGG	9 cut(s)	177, 399, 614, 1144, 2332, 2466, 2488, 2565, 3016
BclI	TGATCA	4 cut(s)	208, 298, 2338, 3168
BcnI	CCSGG	1 cut(s)	1598
BcoDI	GTCTC	6 cut(s)	49, 513, 1388, 2134, 2273, 2718
BfaI	CTAG	5 cut(s)	609, 845, 1743, 1845, 2636
BfmI	CTRYAG	4 cut(s)	483, 1443, 1562, 3042
BfoI	RGCGCY	1 cut(s)	2203
BfuAI	ACCTGC	3 cut(s)	1480, 1844, 3191
BglI	GCCNNNNNGGC	1 cut(s)	1155
BlpI	GCTNAGC	1 cut(s)	1347
Bme18I	GGWCC	2 cut(s)	777, 2747
BmeT110I	CYCGRG	1 cut(s)	1941
BmgBI	CACGTC	1 cut(s)	2144
BmgT120I	GGNCC	4 cut(s)	80, 777, 2425, 2747
BmrI	ACTGGG	3 cut(s)	120, 2766, 3094
BmsI	GCATC	7 cut(s)	362, 1231, 1349, 1680, 2102, 2297, 3187
BmtI	GCTAGC	1 cut(s)	2639
BmuI	ACTGGG	3 cut(s)	120, 2766, 3094
BpiI	GAAGAC	2 cut(s)	1914, 3147
BplI	GAGNNNNNCTC	2 cut(s)	697, 729
BpmI	CTGGAG	4 cut(s)	495, 724, 1165, 3114
Bpu10I	CCTNAGC	2 cut(s)	951, 2441
Bpu1102I	GCTNAGC	1 cut(s)	1347
Bpu14I	TTCGAA	1 cut(s)	1835
BpuEI	CTTGAG	1 cut(s)	1342
BpuMI	CCSGG	1 cut(s)	1598
Bsa29I	ATCGAT	2 cut(s)	2368, 2907
BsaAI	YACGTR	1 cut(s)	2603
BsaBI	GATNNNNATC	1 cut(s)	2173
BsaHI	GRCGYC	2 cut(s)	1513, 1841
BsaI	GGTCTC	1 cut(s)	513
BsaJI	CCNNGG	6 cut(s)	241, 824, 1596, 1940, 2465, 2743
BsaWI	WCCGGW	1 cut(s)	2171
BsaXI	ACNNNNNCTCC	2 cut(s)	2606, 2636
Bsc4I	CCNNNNNNNGG	7 cut(s)	201, 246, 247, 404, 762, 975, 2756
Bse3DI	GCAATG	3 cut(s)	580, 1035, 2868
Bse8I	GATNNNNATC	1 cut(s)	2173
BseAI	TCCGGA	1 cut(s)	2171
BseBI	CCWGG	9 cut(s)	177, 399, 614, 1144, 2332, 2466, 2488, 2565, 3016
BseCI	ATCGAT	2 cut(s)	2368, 2907
BseDI	CCNNGG	6 cut(s)	241, 824, 1596, 1940, 2465, 2743
BseGI	GGATG	6 cut(s)	779, 1189, 1258, 2117, 2766, 2977
BseJI	GATNNNNATC	1 cut(s)	2173
BseLI	CCNNNNNNNGG	7 cut(s)	201, 246, 247, 404, 762, 975, 2756
BseMI	GCAATG	3 cut(s)	580, 1035, 2868
BseMII	CTCAG	7 cut(s)	54, 405, 587, 965, 1334, 2519, 2633
BseRI	GAGGAG	2 cut(s)	2261, 2370
BseXI	GCAGC	9 cut(s)	949, 1349, 1576, 1645, 1939, 2509, 2999, 3056, 3175
BseYI	CCCAGC	3 cut(s)	769, 1315, 1583
BsgI	GTGCAG	4 cut(s)	819, 948, 2615, 3174
Bsh1236I	CGCG	1 cut(s)	67
BshFI	GGCC	6 cut(s)	82, 530, 729, 789, 2427, 2555
BshNI	GGYRCC	1 cut(s)	1555
BshVI	ATCGAT	2 cut(s)	2368, 2907
BsiHKAI	GWGCWC	1 cut(s)	2608
BsiHKCI	CYCGRG	1 cut(s)	1941
BsiSI	CCGG	3 cut(s)	1598, 2172, 2858
BslFI	GGGAC	3 cut(s)	763, 1071, 3023
BslI	CCNNNNNNNGG	7 cut(s)	201, 246, 247, 404, 762, 975, 2756
BsmAI	GTCTC	6 cut(s)	49, 513, 1388, 2134, 2273, 2718
BsmBI	CGTCTC	1 cut(s)	2134
BsmFI	GGGAC	3 cut(s)	763, 1071, 3023
BsmI	GAATGC	3 cut(s)	153, 829, 2491
BsnI	GGCC	6 cut(s)	82, 530, 729, 789, 2427, 2555
Bso31I	GGTCTC	1 cut(s)	513
BsoBI	CYCGRG	1 cut(s)	1941
Bsp119I	TTCGAA	1 cut(s)	1835
Bsp1286I	GDGCHC	2 cut(s)	1151, 2608
Bsp13I	TCCGGA	1 cut(s)	2171
Bsp1720I	GCTNAGC	1 cut(s)	1347
BspACI	CCGC	3 cut(s)	220, 766, 3161
BspANI	GGCC	6 cut(s)	82, 530, 729, 789, 2427, 2555
BspCNI	CTCAG	7 cut(s)	53, 406, 588, 964, 1333, 2518, 2632
BspDI	ATCGAT	2 cut(s)	2368, 2907
BspEI	TCCGGA	1 cut(s)	2171
BspFNI	CGCG	1 cut(s)	67
BspHI	TCATGA	2 cut(s)	385, 1989
BspMAI	CTGCAG	4 cut(s)	487, 1447, 1566, 3046
BspMI	ACCTGC	3 cut(s)	1480, 1844, 3191
BspOI	GCTAGC	1 cut(s)	2639
BspT104I	TTCGAA	1 cut(s)	1835
BspT107I	GGYRCC	1 cut(s)	1555
BspTNI	GGTCTC	1 cut(s)	513
BsrDI	GCAATG	3 cut(s)	580, 1035, 2868
BssECI	CCNNGG	6 cut(s)	241, 824, 1596, 1940, 2465, 2743
BssNI	GRCGYC	2 cut(s)	1513, 1841
BssT1I	CCWWGG	3 cut(s)	241, 824, 2743
Bst2UI	CCWGG	9 cut(s)	177, 399, 614, 1144, 2332, 2466, 2488, 2565, 3016
Bst4CI	ACNGT	8 cut(s)	679, 1069, 1232, 1271, 1399, 1426, 1824, 2065
Bst6I	CTCTTC	4 cut(s)	54, 90, 1256, 3100
BstACI	GRCGYC	2 cut(s)	1513, 1841
BstAPI	GCANNNNNTGC	3 cut(s)	1343, 1427, 3197
BstBAI	YACGTR	1 cut(s)	2603
BstBI	TTCGAA	1 cut(s)	1835
BstC8I	GCNNGC	6 cut(s)	192, 1296, 1353, 2482, 2520, 2637
BstEII	GGTNACC	1 cut(s)	1226
BstENI	CCTNNNNNAGG	2 cut(s)	402, 760
BstF5I	GGATG	6 cut(s)	779, 1189, 1258, 2117, 2766, 2977
BstFNI	CGCG	1 cut(s)	67
BstH2I	RGCGCY	1 cut(s)	2203
BstHHI	GCGC	3 cut(s)	69, 2015, 2202
BstMAI	GTCTC	6 cut(s)	49, 513, 1388, 2134, 2273, 2718
BstNI	CCWGG	9 cut(s)	177, 399, 614, 1144, 2332, 2466, 2488, 2565, 3016
BstNSI	RCATGY	2 cut(s)	1176, 2136
BstPI	GGTNACC	1 cut(s)	1226
BstSFI	CTRYAG	4 cut(s)	483, 1443, 1562, 3042
BstUI	CGCG	1 cut(s)	67
BstV1I	GCAGC	9 cut(s)	949, 1349, 1576, 1645, 1939, 2509, 2999, 3056, 3175
BstV2I	GAAGAC	2 cut(s)	1914, 3147
BstX2I	RGATCY	9 cut(s)	359, 496, 634, 749, 2168, 2231, 2248, 2803, 3157
BstYI	RGATCY	9 cut(s)	359, 496, 634, 749, 2168, 2231, 2248, 2803, 3157
Bsu15I	ATCGAT	2 cut(s)	2368, 2907
BsuRI	GGCC	6 cut(s)	82, 530, 729, 789, 2427, 2555
BsuTUI	ATCGAT	2 cut(s)	2368, 2907
BtgZI	GCGATG	2 cut(s)	1254, 1293
BtrI	CACGTC	1 cut(s)	2144
BtsCI	GGATG	6 cut(s)	779, 1189, 1258, 2117, 2766, 2977
BtsI	GCAGTG	2 cut(s)	430, 585
BtsIMutI	CAGTG	7 cut(s)	195, 411, 430, 538, 585, 1710, 2061
BveI	ACCTGC	3 cut(s)	1480, 1844, 3191
Cac8I	GCNNGC	6 cut(s)	192, 1296, 1353, 2482, 2520, 2637
CaiI	CAGNNNCTG	3 cut(s)	362, 1343, 2990
CciI	TCATGA	2 cut(s)	385, 1989
CfoI	GCGC	3 cut(s)	69, 2015, 2202
Cfr13I	GGNCC	4 cut(s)	80, 777, 2425, 2747
ClaI	ATCGAT	2 cut(s)	2368, 2907
CseI	GACGC	2 cut(s)	1521, 1830
CsiI	ACCWGGT	1 cut(s)	175
Csp6I	GTAC	3 cut(s)	1619, 1916, 2882
CspCI	CAANNNNNGTGG	2 cut(s)	400, 435
CviQI	GTAC	3 cut(s)	1619, 1916, 2882
DraI	TTTAAA	2 cut(s)	2643, 2787
DraIII	CACNNNGTG	1 cut(s)	217
DrdI	GACNNNNNNGTC	1 cut(s)	2035
DseDI	GACNNNNNNGTC	1 cut(s)	2035
EaeI	YGGCCR	4 cut(s)	528, 727, 787, 2553
Eam1104I	CTCTTC	4 cut(s)	54, 90, 1256, 3100
EarI	CTCTTC	4 cut(s)	54, 90, 1256, 3100
EciI	GGCGGA	1 cut(s)	3150
Eco130I	CCWWGG	3 cut(s)	241, 824, 2743
Eco24I	GRGCYC	1 cut(s)	1151
Eco31I	GGTCTC	1 cut(s)	513
Eco32I	GATATC	2 cut(s)	2049, 2851
Eco47I	GGWCC	2 cut(s)	777, 2747
Eco47III	AGCGCT	1 cut(s)	2201
Eco57I	CTGAAG	1 cut(s)	1437
Eco72I	CACGTG	1 cut(s)	2603
Eco88I	CYCGRG	1 cut(s)	1941
Eco91I	GGTNACC	1 cut(s)	1226
EcoNI	CCTNNNNNAGG	2 cut(s)	402, 760
EcoO109I	RGGNCCY	1 cut(s)	80
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRII	CCWGG	9 cut(s)	175, 397, 612, 1142, 2330, 2464, 2486, 2563, 3014
EcoRV	GATATC	2 cut(s)	2049, 2851
EcoT14I	CCWWGG	3 cut(s)	241, 824, 2743
EcoT22I	ATGCAT	1 cut(s)	2117
EcoT38I	GRGCYC	1 cut(s)	1151
ErhI	CCWWGG	3 cut(s)	241, 824, 2743
Esp3I	CGTCTC	1 cut(s)	2134
FalI	AAGNNNNNCTT	6 cut(s)	69, 101, 1100, 1132, 2838, 2870
FaqI	GGGAC	3 cut(s)	763, 1071, 3023
FauI	CCCGC	1 cut(s)	213
FbaI	TGATCA	4 cut(s)	208, 298, 2338, 3168
FokI	GGATG	6 cut(s)	786, 1196, 1265, 2124, 2773, 2964
FriOI	GRGCYC	1 cut(s)	1151
FspBI	CTAG	5 cut(s)	609, 845, 1743, 1845, 2636
FspI	TGCGCA	1 cut(s)	2014
GlaI	GCGC	3 cut(s)	68, 2014, 2201
GsaI	CCCAGC	3 cut(s)	773, 1319, 1587
GsuI	CTGGAG	4 cut(s)	495, 724, 1165, 3114
HaeII	RGCGCY	1 cut(s)	2203
HaeIII	GGCC	6 cut(s)	82, 530, 729, 789, 2427, 2555
HapII	CCGG	3 cut(s)	1598, 2172, 2858
HgaI	GACGC	2 cut(s)	1521, 1830
HhaI	GCGC	3 cut(s)	69, 2015, 2202
Hin1I	GRCGYC	2 cut(s)	1513, 1841
Hin6I	GCGC	3 cut(s)	67, 2013, 2200
HinP1I	GCGC	3 cut(s)	67, 2013, 2200
HpaII	CCGG	3 cut(s)	1598, 2172, 2858
HphI	GGTGA	5 cut(s)	218, 919, 2230, 2525, 2590
Hpy166II	GTNNAC	6 cut(s)	20, 182, 978, 2125, 2720, 2767
Hpy188I	TCNGA	7 cut(s)	118, 364, 597, 667, 1627, 2041, 2920
Hpy8I	GTNNAC	6 cut(s)	20, 182, 978, 2125, 2720, 2767
HpyCH4III	ACNGT	8 cut(s)	679, 1069, 1232, 1271, 1399, 1426, 1824, 2065
HpyCH4IV	ACGT	6 cut(s)	16, 168, 1621, 2143, 2602, 3033
HpySE526I	ACGT	6 cut(s)	16, 168, 1621, 2143, 2602, 3033
Hsp92I	GRCGYC	2 cut(s)	1513, 1841
HspAI	GCGC	3 cut(s)	67, 2013, 2200
Kpn2I	TCCGGA	1 cut(s)	2171
Ksp22I	TGATCA	4 cut(s)	208, 298, 2338, 3168
LmnI	GCTCC	7 cut(s)	650, 705, 1146, 2489, 2571, 3013, 3133
Lsp1109I	GCAGC	9 cut(s)	949, 1349, 1576, 1645, 1939, 2509, 2999, 3056, 3175
LweI	GCATC	7 cut(s)	362, 1231, 1349, 1680, 2102, 2297, 3187
MabI	ACCWGGT	1 cut(s)	175
MaeI	CTAG	5 cut(s)	609, 845, 1743, 1845, 2636
MaeII	ACGT	6 cut(s)	16, 168, 1621, 2143, 2602, 3033
MaeIII	GTNAC	9 cut(s)	602, 625, 698, 1226, 1735, 2022, 2059, 2218, 2578
MfeI	CAATTG	1 cut(s)	639
MflI	RGATCY	9 cut(s)	359, 496, 634, 749, 2168, 2231, 2248, 2803, 3157
MhlI	GDGCHC	2 cut(s)	1151, 2608
MlsI	TGGCCA	3 cut(s)	530, 789, 2555
MluNI	TGGCCA	3 cut(s)	530, 789, 2555
MlyI	GAGTC	6 cut(s)	1057, 2023, 2273, 2720, 2996, 3086
MmeI	TCCRAC	3 cut(s)	110, 2549, 2777
Mox20I	TGGCCA	3 cut(s)	530, 789, 2555
Mph1103I	ATGCAT	1 cut(s)	2117
MroI	TCCGGA	1 cut(s)	2171
MscI	TGGCCA	3 cut(s)	530, 789, 2555
MslI	CAYNNNNRTG	2 cut(s)	1209, 1814
Msp20I	TGGCCA	3 cut(s)	530, 789, 2555
MspA1I	CMGCKG	1 cut(s)	2990
MspI	CCGG	3 cut(s)	1598, 2172, 2858
MunI	CAATTG	1 cut(s)	639
Mva1269I	GAATGC	3 cut(s)	153, 829, 2491
MvaI	CCWGG	9 cut(s)	177, 399, 614, 1144, 2332, 2466, 2488, 2565, 3016
MvnI	CGCG	1 cut(s)	67
NciI	CCSGG	1 cut(s)	1598
NheI	GCTAGC	1 cut(s)	2635
NmeAIII	GCCGAG	1 cut(s)	2774
NmuCI	GTSAC	5 cut(s)	602, 698, 2059, 2218, 2578
NsbI	TGCGCA	1 cut(s)	2014
NsiI	ATGCAT	1 cut(s)	2117
NspI	RCATGY	2 cut(s)	1176, 2136
NspV	TTCGAA	1 cut(s)	1835
PagI	TCATGA	2 cut(s)	385, 1989
PciI	ACATGT	1 cut(s)	2132
PcsI	WCGNNNNNNNCGW	2 cut(s)	1956, 3030
PctI	GAATGC	3 cut(s)	153, 829, 2491
PfeI	GAWTC	5 cut(s)	266, 376, 813, 2327, 2956
PflFI	GACNNNGTC	1 cut(s)	3035
PflMI	CCANNNNNTGG	1 cut(s)	975
PfoI	TCCNGGA	2 cut(s)	1142, 3014
PleI	GAGTC	6 cut(s)	1056, 2023, 2273, 2719, 2996, 3086
PmaCI	CACGTG	1 cut(s)	2603
PmlI	CACGTG	1 cut(s)	2603
PpsI	GAGTC	6 cut(s)	1056, 2023, 2273, 2719, 2996, 3086
Ppu21I	YACGTR	1 cut(s)	2603
PscI	ACATGT	1 cut(s)	2132
PshBI	ATTAAT	1 cut(s)	1652
PsiI	TTATAA	1 cut(s)	1029
Psp6I	CCWGG	9 cut(s)	175, 397, 612, 1142, 2330, 2464, 2486, 2563, 3014
PspCI	CACGTG	1 cut(s)	2603
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	3 cut(s)	769, 1315, 1583
PspGI	CCWGG	9 cut(s)	175, 397, 612, 1142, 2330, 2464, 2486, 2563, 3014
PspPI	GGNCC	4 cut(s)	80, 777, 2425, 2747
PstI	CTGCAG	4 cut(s)	487, 1447, 1566, 3046
PstNI	CAGNNNCTG	3 cut(s)	362, 1343, 2990
PsuI	RGATCY	9 cut(s)	359, 496, 634, 749, 2168, 2231, 2248, 2803, 3157
PsyI	GACNNNGTC	1 cut(s)	3035
PvuII	CAGCTG	1 cut(s)	2990
RsaI	GTAC	3 cut(s)	1620, 1917, 2883
RsaNI	GTAC	3 cut(s)	1619, 1916, 2882
RseI	CAYNNNNRTG	2 cut(s)	1209, 1814
Sau96I	GGNCC	4 cut(s)	80, 777, 2425, 2747
SchI	GAGTC	6 cut(s)	1057, 2023, 2273, 2720, 2996, 3086
SduI	GDGCHC	2 cut(s)	1151, 2608
SexAI	ACCWGGT	1 cut(s)	175
SfaNI	GCATC	7 cut(s)	362, 1231, 1349, 1680, 2102, 2297, 3187
SfcI	CTRYAG	4 cut(s)	483, 1443, 1562, 3042
SfuI	TTCGAA	1 cut(s)	1835
SinI	GGWCC	2 cut(s)	777, 2747
SmiMI	CAYNNNNRTG	2 cut(s)	1209, 1814
SmlI	CTYRAG	1 cut(s)	1357
SmoI	CTYRAG	1 cut(s)	1357
SsiI	CCGC	3 cut(s)	220, 766, 3161
SspI	AATATT	2 cut(s)	940, 2181
SspMI	CTAG	5 cut(s)	609, 845, 1743, 1845, 2636
StyI	CCWWGG	3 cut(s)	241, 824, 2743
TaaI	ACNGT	8 cut(s)	679, 1069, 1232, 1271, 1399, 1426, 1824, 2065
TaiI	ACGT	6 cut(s)	19, 171, 1624, 2146, 2605, 3036
TaqI	TCGA	5 cut(s)	91, 1835, 1936, 2368, 2907
TatI	WGTACW	1 cut(s)	2881
TauI	GCSGC	1 cut(s)	769
TfiI	GAWTC	5 cut(s)	266, 376, 813, 2327, 2956
TscAI	CASTG	7 cut(s)	202, 418, 430, 538, 592, 1717, 2068
TseFI	GTSAC	5 cut(s)	602, 698, 2059, 2218, 2578
TseI	GCWGC	9 cut(s)	962, 1337, 1564, 1658, 1952, 2522, 2987, 3044, 3188
Tsp45I	GTSAC	5 cut(s)	602, 698, 2059, 2218, 2578
TspGWI	ACGGA	2 cut(s)	284, 825
TspRI	CASTG	7 cut(s)	202, 418, 430, 538, 592, 1717, 2068
Tth111I	GACNNNGTC	1 cut(s)	3035
Van91I	CCANNNNNTGG	1 cut(s)	975
VpaK11BI	GGWCC	2 cut(s)	777, 2747
VspI	ATTAAT	1 cut(s)	1652
XagI	CCTNNNNNAGG	2 cut(s)	402, 760
XapI	RAATTY	5 cut(s)	153, 1300, 1479, 2473, 3121
XbaI	TCTAGA	1 cut(s)	844
XceI	RCATGY	2 cut(s)	1176, 2136
XspI	CTAG	5 cut(s)	609, 845, 1743, 1845, 2636
Zsp2I	ATGCAT	1 cut(s)	2117

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.