Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Forward (+)

6061488 .. 6065724

Gene Structure 4237 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGGTGCTGGGTGCCGGCCTAAAGGTCCCAACCTGCTGTACCGCCGCGTATCCGCCACGTTTGAAAATCTCCCCACAACCAGTCTTTTGGCGGAACTCCGCGTCAACCGCGCTTTTGGATTTCTGTGTCTGTATAACCTGTCACTCAGTCACTGTACATTCCAGGAGAGAGAAAAGAAAGAAAAGGATTGAAAGCGAAGAAAGTGGTTTCAGCCCTAAAGCCTCCTCGTTTATTTCCTTCTCTCCAATTCGCTCTCTCACTCCCTTCTTTCACTCCCAAACTCTAAATTGCCGCCGCTCTTGGCTGCTCCCACCACTGCAAGATTTCGCGCTTCGCTTTAGGATCGTGATGTCGAAGTCGGCAATAGCAGTGGGTTTACTGCGTTTGCACAAAAATTGGCCGTGCAGCTCTCCTTTTAGGCTGTTTTGGTCTAAGGGTTCAACTATGGCTGGTGAATTGTTGGTGAGTGCGAATGGAAATGGTAACACCCAGCCCAATCCACAGAGGACTTACCAAGTTGTTGTGGCTGCGACCCGAGAAATGGGTATCGGAAAGGATGGGAAACTACCTTGGAGACTGCCCTCTGATCTCAAGTTCTTTAAGGACGTCACTGTGACCACATCAGATCCTGTGAAAAAGAATGCAATTATAATGGGTAGAAAAACATGGGAGAGCATTCCATCTGAGCATCAACCTCTACCTGGCCGCCTTAATGTTGTTCTGACTCGTTCTGGGAGTTTTGATATTGCTACTGCAGAGAATGTTGTGATATGCGGAAGCATGGCATCTGCTTTGGAATTGTTAGCTGCTTCTCCTTACTGTCTATCAATTGAGAGAGTGTTTCTCATTGGAGGTGGCCAGATACTAAGGGAAGCTCTCAATGCGCCTGGCTGTGAAGCCATTCACATTACAGAAATAGAGACAAACATTGAATGTGACACTTTTATCCCTGCAATTGATTCCTCTGTGTTTCAGCCGTGGTATTCATCCTTTCCCAAGGTTGAAAATAGCATTCGTCATTCTTTCACAACTTACGTTCGCGTAAGGAGTTCTGCAGTTGAATCCCTTTGTGAAAACAGTGATCTGATCTCTAATAGTAAGTCAGGCTCCATTAAATGTGAGGTGAAGAATTTCTCTTTTCTGCCAAAGATGATATTTGAGAAACACGAGGAGCAGATGTATCTAAGGCTGGTACGAGAAATACTCTCAGATGGCACTTCTAAGGATAACAGGACAGGGACTGGTACTTTGTCAAAATTCGGTTGCCAGATGCGGTTTAACCTGCGCAGAACTTTTCCACTTCTGACAACCAAGAAAGTATTTTGGCGAGGGGTTGTTGAAGAACTTCTGTGGTTCATCAGTGGTTCAACAAATGCCAAGGTCCTTCAGGAGAAAGGTATTCACATATGGGATGGAAATGCATCTAGAGACTACCTTGACAGTGTTGGTTTAACTGACAAGGAAGAGGGCGACTTGGGACCAGTATATGGGTTCCAGTGGCGACACTTTGGTGCCAGGTATACTGACATGCATGCTGACTATACCGATCAAGGATTTGATCAGTTGCTAGATGTTATTGACAAGATTAAAAATAATCCAGATGATCGGCGGATTATACTGTCAGCATGGAATCCTTCTGATCTCAAATTGATGGCACTCCCACCTTGTCACATGTTTGCTCAGTTCTATGTAGCCAACGGAGAGTTATCATGTCAAATGTATCAGCGTTCTGCTGACATCGGCCTTGGTGTTCCATTTAACATTGCATCATATGCTCTTCTGACATGCATGATTGCTCATATTTGTGGTCTTGTTCCAGGTGATTTCATTCATGCCTTAGGGGATGCTCATGTTTACCGCACTCACATCAGGCCTTTGAAGGAGCAGCTTGAAAAGCTGCCCAAGCCTTTTCCAATTTTGAAGATCGATCCTGAGAAAAAGAATATAGATTCTTTTGTGGCGGCTGATTTCAAACTGTTAGGTTATCGATCCTCATCAGAAGATAGAAATGAAAATGGCCGTATAGGGCTCTCTCAAATTTTCAGTAGAGAGCTCAGACACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

688

Amino Acids

77.17

Weight (kDa)

8.95

Isoelectric Point (pI)

51.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	172 - 347	2.4e-36	Dihydrofolate reductase
Thymidylat_synt	PF00303	392 - 666	1.9e-110	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	650
AatII	GACGTC	1 cut(s)	609
Acc16I	TGCGCA	1 cut(s)	1286
Acc36I	ACCTGC	2 cut(s)	41, 1290
AccB1I	GGYRCC	2 cut(s)	11, 1511
AccB7I	CCANNNNNTGG	1 cut(s)	1487
AccBSI	CCGCTC	1 cut(s)	297
AccI	GTMKAC	1 cut(s)	1520
AccII	CGCG	5 cut(s)	47, 101, 110, 329, 1041
AclWI	GGATC	4 cut(s)	350, 620, 1922, 1983
AcoI	YGGCCR	4 cut(s)	398, 703, 856, 2017
AcsI	RAATTY	3 cut(s)	1129, 1256, 2037
AcuI	CTGAAG	1 cut(s)	1370
AcyI	GRCGYC	1 cut(s)	606
AfaI	GTAC	4 cut(s)	40, 156, 1194, 1246
AfiI	CCNNNNNNNGG	3 cut(s)	541, 701, 1487
AflIII	ACRYGT	1 cut(s)	1671
AjnI	CCWGG	5 cut(s)	161, 700, 886, 1514, 1816
AleI	CACNNNNGTG	1 cut(s)	1509
AluBI	AGCT	6 cut(s)	408, 806, 875, 1888, 1897, 2053
AluI	AGCT	6 cut(s)	408, 806, 875, 1888, 1897, 2053
Alw21I	GWGCWC	1 cut(s)	2055
Alw26I	GTCTC	3 cut(s)	568, 914, 1422
AlwI	GGATC	4 cut(s)	350, 620, 1922, 1983
AlwNI	CAGNNNCTG	3 cut(s)	152, 629, 1241
Ama87I	CYCGRG	1 cut(s)	534
AoxI	GGCC	7 cut(s)	16, 398, 703, 856, 1741, 1871, 2017
ApeKI	GCWGC	6 cut(s)	304, 405, 527, 806, 1885, 1897
ApoI	RAATTY	3 cut(s)	1129, 1256, 2037
ArsI	GACNNNNNNTTYG	2 cut(s)	1231, 1263
Asp700I	GAANNNNTTC	3 cut(s)	900, 1130, 1344
AspLEI	GCGC	4 cut(s)	112, 331, 886, 1287
AspS9I	GGNCC	3 cut(s)	25, 1381, 1478
AsuHPI	GGTGA	4 cut(s)	464, 475, 1135, 1832
AvaI	CYCGRG	1 cut(s)	534
AvaII	GGWCC	3 cut(s)	25, 1381, 1478
AxyI	CCTNAGG	1 cut(s)	1837
BalI	TGGCCA	1 cut(s)	858
BanI	GGYRCC	2 cut(s)	11, 1511
BanII	GRGCYC	2 cut(s)	2031, 2055
BauI	CACGAG	1 cut(s)	1166
Bbv12I	GWGCWC	1 cut(s)	2055
BbvI	GCAGC	6 cut(s)	291, 417, 514, 793, 1884, 1897
BccI	CCATC	5 cut(s)	551, 688, 1205, 1406, 1645
BceAI	ACGGC	3 cut(s)	385, 961, 2004
BciT130I	CCWGG	5 cut(s)	163, 702, 888, 1516, 1818
BciVI	GTATCC	1 cut(s)	60
BclI	TGATCA	1 cut(s)	1558
BcoDI	GTCTC	3 cut(s)	568, 914, 1422
BfaI	CTAG	3 cut(s)	1425, 1568, 2062
BfmI	CTRYAG	2 cut(s)	753, 1053
BfuAI	ACCTGC	2 cut(s)	41, 1290
BfuI	GTATCC	1 cut(s)	60
Bme1390I	CCNGG	5 cut(s)	163, 702, 888, 1516, 1818
Bme18I	GGWCC	3 cut(s)	25, 1381, 1478
BmeT110I	CYCGRG	1 cut(s)	534
BmgT120I	GGNCC	3 cut(s)	25, 1381, 1478
BmiI	GGNNCC	6 cut(s)	13, 27, 1108, 1479, 1493, 1513
BmrFI	CCNGG	5 cut(s)	163, 702, 888, 1516, 1818
BmsI	GCATC	6 cut(s)	697, 794, 1260, 1430, 1775, 1834
BplI	GAGNNNNNCTC	4 cut(s)	828, 860, 1188, 1220
BpuEI	CTTGAG	1 cut(s)	575
Bsa29I	ATCGAT	2 cut(s)	1926, 1987
BsaBI	GATNNNNATC	1 cut(s)	1993
BsaHI	GRCGYC	1 cut(s)	606
BsaJI	CCNNGG	5 cut(s)	569, 977, 996, 1377, 1744
BsaXI	ACNNNNNCTCC	2 cut(s)	1163, 1193
Bsc4I	CCNNNNNNNGG	3 cut(s)	541, 701, 1487
Bse118I	RCCGGY	1 cut(s)	14
Bse1I	ACTGG	4 cut(s)	80, 1246, 1481, 1495
Bse21I	CCTNAGG	1 cut(s)	1837
Bse3DI	GCAATG	1 cut(s)	1761
Bse8I	GATNNNNATC	1 cut(s)	1993
BseBI	CCWGG	5 cut(s)	163, 702, 888, 1516, 1818
BseCI	ATCGAT	2 cut(s)	1926, 1987
BseDI	CCNNGG	5 cut(s)	569, 977, 996, 1377, 1744
BseGI	GGATG	4 cut(s)	562, 986, 1417, 1849
BseJI	GATNNNNATC	1 cut(s)	1993
BseLI	CCNNNNNNNGG	3 cut(s)	541, 701, 1487
BseMI	GCAATG	1 cut(s)	1761
BseMII	CTCAG	5 cut(s)	159, 675, 1221, 1694, 1923
BseNI	ACTGG	4 cut(s)	80, 1246, 1481, 1495
BseRI	GAGGAG	2 cut(s)	214, 1184
BseXI	GCAGC	6 cut(s)	291, 417, 514, 793, 1884, 1897
BseYI	CCCAGC	2 cut(s)	7, 489
BsgI	GTGCAG	1 cut(s)	424
Bsh1236I	CGCG	5 cut(s)	47, 101, 110, 329, 1041
BshFI	GGCC	7 cut(s)	18, 400, 705, 858, 1743, 1873, 2019
BshNI	GGYRCC	2 cut(s)	11, 1511
BshVI	ATCGAT	2 cut(s)	1926, 1987
BsiHKAI	GWGCWC	1 cut(s)	2055
BsiHKCI	CYCGRG	1 cut(s)	534
BsiSI	CCGG	1 cut(s)	15
BslFI	GGGAC	3 cut(s)	11, 1252, 1491
BslI	CCNNNNNNNGG	3 cut(s)	541, 701, 1487
BsmAI	GTCTC	3 cut(s)	568, 914, 1422
BsmFI	GGGAC	3 cut(s)	11, 1252, 1491
BsmI	GAATGC	3 cut(s)	646, 675, 1011
BsnI	GGCC	7 cut(s)	18, 400, 705, 858, 1743, 1873, 2019
BsoBI	CYCGRG	1 cut(s)	534
Bsp1286I	GDGCHC	2 cut(s)	2031, 2055
Bsp1407I	TGTACA	1 cut(s)	154
BspANI	GGCC	7 cut(s)	18, 400, 705, 858, 1743, 1873, 2019
BspCNI	CTCAG	5 cut(s)	158, 676, 1220, 1693, 1924
BspDI	ATCGAT	2 cut(s)	1926, 1987
BspFNI	CGCG	5 cut(s)	47, 101, 110, 329, 1041
BspLI	GGNNCC	6 cut(s)	13, 27, 1108, 1479, 1493, 1513
BspMAI	CTGCAG	2 cut(s)	757, 1057
BspMI	ACCTGC	2 cut(s)	41, 1290
BspPI	GGATC	4 cut(s)	350, 620, 1922, 1983
BspQI	GCTCTTC	1 cut(s)	1782
BspT107I	GGYRCC	2 cut(s)	11, 1511
BsrBI	CCGCTC	1 cut(s)	297
BsrDI	GCAATG	1 cut(s)	1761
BsrFI	RCCGGY	1 cut(s)	14
BsrGI	TGTACA	1 cut(s)	154
BsrI	ACTGG	4 cut(s)	80, 1246, 1481, 1495
BssAI	RCCGGY	1 cut(s)	14
BssECI	CCNNGG	5 cut(s)	569, 977, 996, 1377, 1744
BssNAI	GTATAC	1 cut(s)	1521
BssNI	GRCGYC	1 cut(s)	606
BssSI	CACGAG	1 cut(s)	1166
BssT1I	CCWWGG	4 cut(s)	569, 996, 1377, 1744
Bst1107I	GTATAC	1 cut(s)	1521
Bst2BI	CACGAG	1 cut(s)	1166
Bst2UI	CCWGG	5 cut(s)	163, 702, 888, 1516, 1818
Bst4CI	ACNGT	7 cut(s)	154, 613, 821, 1079, 1442, 1620, 1977
Bst6I	CTCTTC	2 cut(s)	1458, 1782
BstACI	GRCGYC	1 cut(s)	606
BstAPI	GCANNNNNTGC	1 cut(s)	1772
BstAUI	TGTACA	1 cut(s)	154
BstC8I	GCNNGC	2 cut(s)	16, 1533
BstDSI	CCRYGG	1 cut(s)	977
BstF5I	GGATG	4 cut(s)	562, 986, 1417, 1849
BstFNI	CGCG	5 cut(s)	47, 101, 110, 329, 1041
BstHHI	GCGC	4 cut(s)	112, 331, 886, 1287
BstMAI	GTCTC	3 cut(s)	568, 914, 1422
BstMWI	GCNNNNNNNGC	5 cut(s)	107, 881, 1772, 1894, 1903
BstNI	CCWGG	5 cut(s)	163, 702, 888, 1516, 1818
BstNSI	RCATGY	4 cut(s)	1531, 1535, 1675, 1788
BstSCI	CCNGG	5 cut(s)	161, 700, 886, 1514, 1816
BstSFI	CTRYAG	2 cut(s)	753, 1053
BstUI	CGCG	5 cut(s)	47, 101, 110, 329, 1041
BstV1I	GCAGC	6 cut(s)	291, 417, 514, 793, 1884, 1897
BstX2I	RGATCY	1 cut(s)	625
BstXI	CCANNNNNNTGG	1 cut(s)	87
BstYI	RGATCY	1 cut(s)	625
BstZ17I	GTATAC	1 cut(s)	1521
Bsu15I	ATCGAT	2 cut(s)	1926, 1987
Bsu36I	CCTNAGG	1 cut(s)	1837
BsuI	GTATCC	1 cut(s)	60
BsuRI	GGCC	7 cut(s)	18, 400, 705, 858, 1743, 1873, 2019
BsuTUI	ATCGAT	2 cut(s)	1926, 1987
BtgI	CCRYGG	1 cut(s)	977
BtsCI	GGATG	4 cut(s)	562, 986, 1417, 1849
BtsI	GCAGTG	2 cut(s)	314, 375
BtsIMutI	CAGTG	8 cut(s)	150, 314, 375, 609, 1084, 1366, 1447, 1502
BveI	ACCTGC	2 cut(s)	41, 1290
Cac8I	GCNNGC	2 cut(s)	16, 1533
CaiI	CAGNNNCTG	3 cut(s)	152, 629, 1241
CfoI	GCGC	4 cut(s)	112, 331, 886, 1287
Cfr10I	RCCGGY	1 cut(s)	14
Cfr13I	GGNCC	3 cut(s)	25, 1381, 1478
ClaI	ATCGAT	2 cut(s)	1926, 1987
CseI	GACGC	1 cut(s)	90
Csp6I	GTAC	4 cut(s)	39, 155, 1193, 1245
CspCI	CAANNNNNGTGG	2 cut(s)	351, 386
CviQI	GTAC	4 cut(s)	39, 155, 1193, 1245
EaeI	YGGCCR	4 cut(s)	398, 703, 856, 2017
Eam1104I	CTCTTC	2 cut(s)	1458, 1782
EarI	CTCTTC	2 cut(s)	1458, 1782
EciI	GGCGGA	3 cut(s)	42, 106, 1624
Ecl136II	GAGCTC	1 cut(s)	2053
Eco130I	CCWWGG	4 cut(s)	569, 996, 1377, 1744
Eco147I	AGGCCT	1 cut(s)	1873
Eco24I	GRGCYC	2 cut(s)	2031, 2055
Eco47I	GGWCC	3 cut(s)	25, 1381, 1478
Eco53kI	GAGCTC	1 cut(s)	2053
Eco57I	CTGAAG	1 cut(s)	1370
Eco81I	CCTNAGG	1 cut(s)	1837
Eco88I	CYCGRG	1 cut(s)	534
EcoICRI	GAGCTC	1 cut(s)	2053
EcoO109I	RGGNCCY	2 cut(s)	25, 1381
EcoRII	CCWGG	5 cut(s)	161, 700, 886, 1514, 1816
EcoT14I	CCWWGG	4 cut(s)	569, 996, 1377, 1744
EcoT22I	ATGCAT	3 cut(s)	1423, 1533, 1790
EcoT38I	GRGCYC	2 cut(s)	2031, 2055
ErhI	CCWWGG	4 cut(s)	569, 996, 1377, 1744
FaqI	GGGAC	3 cut(s)	11, 1252, 1491
FauNDI	CATATG	2 cut(s)	1406, 1771
FbaI	TGATCA	1 cut(s)	1558
FblI	GTMKAC	1 cut(s)	1520
FokI	GGATG	4 cut(s)	569, 973, 1424, 1856
FriOI	GRGCYC	2 cut(s)	2031, 2055
FspBI	CTAG	3 cut(s)	1425, 1568, 2062
FspI	TGCGCA	1 cut(s)	1286
GlaI	GCGC	4 cut(s)	111, 330, 885, 1286
GsaI	CCCAGC	2 cut(s)	11, 493
HaeIII	GGCC	7 cut(s)	18, 400, 705, 858, 1743, 1873, 2019
HapII	CCGG	1 cut(s)	15
HgaI	GACGC	1 cut(s)	90
HhaI	GCGC	4 cut(s)	112, 331, 886, 1287
Hin1I	GRCGYC	1 cut(s)	606
Hin6I	GCGC	4 cut(s)	110, 329, 884, 1285
HinP1I	GCGC	4 cut(s)	110, 329, 884, 1285
HincII	GTYRAC	1 cut(s)	105
HindII	GTYRAC	1 cut(s)	105
HinfI	GANTC	5 cut(s)	724, 959, 1061, 1630, 1949
HpaII	CCGG	1 cut(s)	15
HphI	GGTGA	4 cut(s)	464, 475, 1135, 1832
Hpy166II	GTNNAC	4 cut(s)	105, 377, 1521, 1855
Hpy188III	TCNNGA	5 cut(s)	346, 1388, 1425, 1598, 1931
Hpy8I	GTNNAC	4 cut(s)	105, 377, 1521, 1855
HpyAV	CCTTC	5 cut(s)	247, 274, 1394, 1644, 1873
HpyCH4III	ACNGT	7 cut(s)	154, 613, 821, 1079, 1442, 1620, 1977
HpyCH4IV	ACGT	3 cut(s)	58, 606, 1035
HpyF10VI	GCNNNNNNNGC	5 cut(s)	107, 881, 1772, 1894, 1903
HpySE526I	ACGT	3 cut(s)	58, 606, 1035
Hsp92I	GRCGYC	1 cut(s)	606
HspAI	GCGC	4 cut(s)	110, 329, 884, 1285
KroI	GCCGGC	1 cut(s)	14
KroNI	GCCGGC	1 cut(s)	16
Ksp22I	TGATCA	1 cut(s)	1558
LguI	GCTCTTC	1 cut(s)	1782
LmnI	GCTCC	4 cut(s)	312, 1112, 1171, 1882
Lsp1109I	GCAGC	6 cut(s)	291, 417, 514, 793, 1884, 1897
LweI	GCATC	6 cut(s)	697, 794, 1260, 1430, 1775, 1834
MaeI	CTAG	3 cut(s)	1425, 1568, 2062
MaeII	ACGT	3 cut(s)	58, 606, 1035
MaeIII	GTNAC	7 cut(s)	140, 148, 482, 607, 613, 935, 1667
MbiI	CCGCTC	1 cut(s)	297
MboII	GAAGA	7 cut(s)	209, 1138, 1352, 1475, 1769, 1933, 2012
MfeI	CAATTG	2 cut(s)	828, 954
MflI	RGATCY	1 cut(s)	625
MhlI	GDGCHC	2 cut(s)	2031, 2055
MlsI	TGGCCA	1 cut(s)	858
MluNI	TGGCCA	1 cut(s)	858
MlyI	GAGTC	1 cut(s)	718
Mox20I	TGGCCA	1 cut(s)	858
Mph1103I	ATGCAT	3 cut(s)	1423, 1533, 1790
MroNI	GCCGGC	1 cut(s)	14
MroXI	GAANNNNTTC	3 cut(s)	900, 1130, 1344
MscI	TGGCCA	1 cut(s)	858
MseI	TTAA	7 cut(s)	600, 711, 1113, 1278, 1451, 1587, 1758
MslI	CAYNNNNRTG	2 cut(s)	1509, 1803
Msp20I	TGGCCA	1 cut(s)	858
MspI	CCGG	1 cut(s)	15
MspR9I	CCNGG	5 cut(s)	163, 702, 888, 1516, 1818
MunI	CAATTG	2 cut(s)	828, 954
Mva1269I	GAATGC	3 cut(s)	646, 675, 1011
MvaI	CCWGG	5 cut(s)	163, 702, 888, 1516, 1818
MvnI	CGCG	5 cut(s)	47, 101, 110, 329, 1041
MwoI	GCNNNNNNNGC	5 cut(s)	107, 881, 1772, 1894, 1903
NaeI	GCCGGC	1 cut(s)	16
NdeI	CATATG	2 cut(s)	1406, 1771
NgoMIV	GCCGGC	1 cut(s)	14
NlaIV	GGNNCC	6 cut(s)	13, 27, 1108, 1479, 1493, 1513
NmuCI	GTSAC	6 cut(s)	140, 148, 607, 613, 935, 1667
NsbI	TGCGCA	1 cut(s)	1286
NsiI	ATGCAT	3 cut(s)	1423, 1533, 1790
NspI	RCATGY	4 cut(s)	1531, 1535, 1675, 1788
OliI	CACNNNNGTG	1 cut(s)	1509
PaeI	GCATGC	1 cut(s)	1535
PceI	AGGCCT	1 cut(s)	1873
PciI	ACATGT	1 cut(s)	1671
PciSI	GCTCTTC	1 cut(s)	1782
PctI	GAATGC	3 cut(s)	646, 675, 1011
PdiI	GCCGGC	1 cut(s)	16
PdmI	GAANNNNTTC	3 cut(s)	900, 1130, 1344
PfeI	GAWTC	4 cut(s)	959, 1061, 1630, 1949
PflMI	CCANNNNNTGG	1 cut(s)	1487
PfoI	TCCNGGA	1 cut(s)	161
PleI	GAGTC	1 cut(s)	718
PpsI	GAGTC	1 cut(s)	718
PpuMI	RGGWCCY	2 cut(s)	25, 1381
PscI	ACATGT	1 cut(s)	1671
PsiI	TTATAA	1 cut(s)	650
Psp124BI	GAGCTC	1 cut(s)	2055
Psp5II	RGGWCCY	2 cut(s)	25, 1381
Psp6I	CCWGG	5 cut(s)	161, 700, 886, 1514, 1816
PspFI	CCCAGC	2 cut(s)	7, 489
PspGI	CCWGG	5 cut(s)	161, 700, 886, 1514, 1816
PspN4I	GGNNCC	6 cut(s)	13, 27, 1108, 1479, 1493, 1513
PspPI	GGNCC	3 cut(s)	25, 1381, 1478
PspPPI	RGGWCCY	2 cut(s)	25, 1381
PstI	CTGCAG	2 cut(s)	757, 1057
PstNI	CAGNNNCTG	3 cut(s)	152, 629, 1241
PsuI	RGATCY	1 cut(s)	625
RsaI	GTAC	4 cut(s)	40, 156, 1194, 1246
RsaNI	GTAC	4 cut(s)	39, 155, 1193, 1245
RseI	CAYNNNNRTG	2 cut(s)	1509, 1803
SacI	GAGCTC	1 cut(s)	2055
SapI	GCTCTTC	1 cut(s)	1782
SaqAI	TTAA	7 cut(s)	600, 711, 1113, 1278, 1451, 1587, 1758
Sau96I	GGNCC	3 cut(s)	25, 1381, 1478
SchI	GAGTC	1 cut(s)	718
ScrFI	CCNGG	5 cut(s)	163, 702, 888, 1516, 1818
SduI	GDGCHC	2 cut(s)	2031, 2055
SfaNI	GCATC	6 cut(s)	697, 794, 1260, 1430, 1775, 1834
SfcI	CTRYAG	2 cut(s)	753, 1053
SinI	GGWCC	3 cut(s)	25, 1381, 1478
SmiMI	CAYNNNNRTG	2 cut(s)	1509, 1803
SmlI	CTYRAG	1 cut(s)	590
SmoI	CTYRAG	1 cut(s)	590
SphI	GCATGC	1 cut(s)	1535
SseBI	AGGCCT	1 cut(s)	1873
SspMI	CTAG	3 cut(s)	1425, 1568, 2062
SstI	GAGCTC	1 cut(s)	2055
StuI	AGGCCT	1 cut(s)	1873
StyD4I	CCNGG	5 cut(s)	161, 700, 886, 1514, 1816
StyI	CCWWGG	4 cut(s)	569, 996, 1377, 1744
TaaI	ACNGT	7 cut(s)	154, 613, 821, 1079, 1442, 1620, 1977
TaiI	ACGT	3 cut(s)	61, 609, 1038
TaqI	TCGA	3 cut(s)	353, 1926, 1987
TatI	WGTACW	1 cut(s)	154
TauI	GCSGC	5 cut(s)	47, 294, 297, 708, 1964
TfiI	GAWTC	4 cut(s)	959, 1061, 1630, 1949
Tru1I	TTAA	7 cut(s)	600, 711, 1113, 1278, 1451, 1587, 1758
Tru9I	TTAA	7 cut(s)	600, 711, 1113, 1278, 1451, 1587, 1758
TscAI	CASTG	8 cut(s)	157, 321, 375, 616, 1084, 1366, 1447, 1502
TseFI	GTSAC	6 cut(s)	140, 148, 607, 613, 935, 1667
TseI	GCWGC	6 cut(s)	304, 405, 527, 806, 1885, 1897
Tsp45I	GTSAC	6 cut(s)	140, 148, 607, 613, 935, 1667
TspDTI	ATGAA	5 cut(s)	975, 1345, 1816, 1820, 2025
TspGWI	ACGGA	1 cut(s)	1713
TspRI	CASTG	8 cut(s)	157, 321, 375, 616, 1084, 1366, 1447, 1502
Van91I	CCANNNNNTGG	1 cut(s)	1487
VpaK11BI	GGWCC	3 cut(s)	25, 1381, 1478
XapI	RAATTY	3 cut(s)	1129, 1256, 2037
XbaI	TCTAGA	1 cut(s)	1424
XceI	RCATGY	4 cut(s)	1531, 1535, 1675, 1788
XmiI	GTMKAC	1 cut(s)	1520
XmnI	GAANNNNTTC	3 cut(s)	900, 1130, 1344
XspI	CTAG	3 cut(s)	1425, 1568, 2062
ZraI	GACGTC	1 cut(s)	607
Zsp2I	ATGCAT	3 cut(s)	1423, 1533, 1790

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.