pycom10g12150

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003349
Physical Location & Seq
Forward (+)
15729119 .. 15734149
5031 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1863 bp
ATGGCCTCCATCCTCTCCAAGAAGCAGAAGCAGAAGCAAAAAAGCACCAAGCTTTCCGACCTAAAAACACTAGGGAACCAGCTCCTCTCGTCCCGGGCCCACATCAACAACCTCCCTAAGCTTCTCTCATTCGTCTCCCCGGCCTCGCCGCCTCAGTTCGTCGTCGAGTCCCTCCTCTCTCTCCAGTCCTTCTTTACCCCTCTCCTTCCCGACCTACCTCCCTCCTCCTCCAAGCCTTCCGCCGCCGATGGCTCCCATGACGACCCCGACTTCATATACCGCACCTGGCTCCGCTCCAAGTTCGACGAGCTCGTTGAGGCCCTCGTCGAAGTCCTGCTTTCGCCGCAATCTGACGAAACTCTCAAGGAGGTGGTGTTGGACACGATAATGGAGTTTGTGAAGTTCGGAAATGGCGGAAAGTTTCACTCTGCAATGTACCATAGGCTGCTTCGTAGTATTGTTTACACTACAGAACCGGTTAATTTGTTGTTAGACTTGCTCTCATCCAAGTATTTCAAGTATATCGATGTCCGTTATTTTACCTACATTAGCTTTGAAAAGCTTGCTAAAATTCTGGATGCAAACGGCATTTCTGAGGACAAAAACGTGAAGAAAGACGGCAATGATGGGGAGCATCCCAGCAAAAGCATGGATCAAGTAATTCACAAGATTCATTATTTGATATCACACATCCCATCTCTGGAAAGCTCAGTTGAAAAAACTGATCATGAGATGTGGAGTGGATCAGGTGAATATGAAGGGCTTGCTGGGAAGATGAAGGCAGATGGTAAACAGCACAAGACTGAGAAGCAGAAGGATAAGGTCTTGTCTGCGGACAATATTGCCAAAAAGATGAAATTGAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCAATTGATGTGTACAAGGAGGTTCTTGCCACTCTCCATCAGGCAGTCATTCCTTTCTTAGCCAACCCCGTCTTGTTATGTGACTTCTTAACTCGATCATATGACATTGGTGGTGTTATTAGTGTCATGGCTCTTAGCAGTCTCTTTATCCTCATGACACAACATGGTTTAGAGTACCCCAACTTTTATGAGAAACTTTATGCATTACTGGTTCCCTCTATTTTTATGGCAAAGCATCGATCAAAGTTTTTTCAGCTTGTAGATGCTTGCTTAAAATCACCACTTCTTCCTGCATATTTGGCTGCTGCTTTTGCCAAGAAATTGAGTAGGCTGTCAATCTCAGTTCCTCCGTCTGGAGCATTAGTTATTATTGTGCTGGTTCATAATCTTCTACGGAGACATCCTTCAATCAATTGCTTGGTGAATCGGGGAGGTGGTGGTGAAACTGTGAAAGATGATCCAGAAGCAGAACAGAGGGCTGCTGATGGCGCCGATGGCACTGGAACTGGTTCAGCAGATAAGTCAATCAAAAAATCAGGCATGGATCCTTTTGATAATGAACAAAGTGATCCTATGAAATCAAATGCCATGAGAAGCTCACTTTGGGAGATTGATACCCTCCGTCACCACTACTGCCCTGCTGTTTCAAGGTTTGTTTTGTCACTCGAGAATGATTTGACAGTCAGAGCCAAAACCACTGAAATATCTGTTGAAGATTTTAGCTCAGGTTCATATGCGACAATATTTGGAGAGCAGGTGAGACGAAGGATAAAGTCAGCTCCACTAGCATACTACAAAGCAGCTCCGACCTCTTTGTTCTCAGACTCCGAGTCTCAGTTTCCCGGTTGGACTTTCACGTGTGAAGACAGTAAGCGAAAGAACGACAGCGCTGATGAGAACGGGAAAATAGATAAAGAACGTGACCATAGTCCGGTCAAACGACAACGCGTCAAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

621

Amino Acids

69.32

Weight (kDa)

8.74

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 342 - 520 1.6e-46 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1648
Acc36I ACCTGC 1 cut(s) 1648
AccB1I GGYRCC 1 cut(s) 1391
AccBSI CCGCTC 1 cut(s) 294
AccII CGCG 1 cut(s) 1851
AciI CCGC 8 cut(s) 149, 240, 243, 280, 292, 344, 414, 833
AclWI GGATC 6 cut(s) 660, 751, 1355, 1442, 1455, 1466
AcsI RAATTY 2 cut(s) 570, 863
AcvI CACGTG 1 cut(s) 1761
AcyI GRCGYC 1 cut(s) 1392
AfaI GTAC 3 cut(s) 437, 917, 1079
AfeI AGCGCT 1 cut(s) 1792
AfiI CCNNNNNNNGG 3 cut(s) 700, 1256, 1834
AflIII ACRYGT 2 cut(s) 1760, 1849
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 7 cut(s) 517, 557, 716, 862, 1311, 1551, 1616
AhdI GACNNNNNGTC 2 cut(s) 1732, 1850
AjnI CCWGG 1 cut(s) 284
AjuI GAANNNNNNNTTGG 2 cut(s) 1489, 1521
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 5 cut(s) 139, 1049, 1294, 1657, 1740
AlwI GGATC 6 cut(s) 660, 751, 1355, 1442, 1455, 1466
Ama87I CYCGRG 2 cut(s) 93, 1568
Aor51HI AGCGCT 1 cut(s) 1792
AoxI GGCC 4 cut(s) 3, 96, 141, 318
ApaI GGGCCC 1 cut(s) 100
ApeKI GCWGC 5 cut(s) 445, 1205, 1208, 1382, 1703
ApoI RAATTY 2 cut(s) 570, 863
AsiGI ACCGGT 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 1411
AspLEI GCGC 2 cut(s) 1394, 1793
AspS9I GGNCC 3 cut(s) 96, 97, 319
AsuC2I CCSGG 4 cut(s) 94, 95, 140, 1746
AsuHPI GGTGA 6 cut(s) 761, 1173, 1336, 1355, 1520, 1672
AvaI CYCGRG 2 cut(s) 93, 1568
BaeGI GKGCMC 1 cut(s) 100
BamHI GGATCC 1 cut(s) 1447
BanI GGYRCC 1 cut(s) 1391
BanII GRGCYC 2 cut(s) 100, 312
BbrPI CACGTG 1 cut(s) 1761
BbsI GAAGAC 1 cut(s) 1773
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 432, 1192, 1195, 1369, 1715
BccI CCATC 8 cut(s) 17, 242, 620, 703, 779, 948, 1382, 1391
BceAI ACGGC 2 cut(s) 601, 634
BciT130I CCWGG 1 cut(s) 286
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 4 cut(s) 94, 95, 140, 1746
BcoDI GTCTC 5 cut(s) 139, 1049, 1294, 1657, 1740
BfaI CTAG 2 cut(s) 71, 1688
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 2 cut(s) 1395, 1794
BfuAI ACCTGC 1 cut(s) 1648
BisI GCNGC 8 cut(s) 149, 243, 344, 446, 1206, 1209, 1383, 1704
BlsI GCNGC 8 cut(s) 150, 244, 345, 447, 1207, 1210, 1384, 1705
Bme1390I CCNGG 5 cut(s) 94, 95, 140, 286, 1746
BmeRI GACNNNNNGTC 2 cut(s) 1732, 1850
BmeT110I CYCGRG 2 cut(s) 93, 1568
BmgT120I GGNCC 3 cut(s) 96, 97, 319
BmiI GGNNCC 7 cut(s) 77, 98, 253, 290, 1116, 1393, 1449
BmrFI CCNGG 5 cut(s) 94, 95, 140, 286, 1746
BmsI GCATC 4 cut(s) 568, 643, 1147, 1156
BoxI GACNNNNGTC 1 cut(s) 1830
BpiI GAAGAC 1 cut(s) 1773
BpmI CTGGAG 2 cut(s) 167, 1278
Bpu10I CCTNAGC 2 cut(s) 117, 1627
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 4 cut(s) 94, 95, 140, 1746
Bsa29I ATCGAT 2 cut(s) 525, 1141
BsaAI YACGTR 1 cut(s) 1761
BsaHI GRCGYC 1 cut(s) 1392
BsaJI CCNNGG 2 cut(s) 93, 138
BsaWI WCCGGW 2 cut(s) 475, 1834
BsaXI ACNNNNNCTCC 6 cut(s) 359, 389, 1251, 1281, 1644, 1674
Bsc4I CCNNNNNNNGG 3 cut(s) 700, 1256, 1834
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 4 cut(s) 184, 1116, 1408, 1414
Bse3DI GCAATG 2 cut(s) 438, 628
BseBI CCWGG 1 cut(s) 286
BseCI ATCGAT 2 cut(s) 525, 1141
BseDI CCNNGG 2 cut(s) 93, 138
BseGI GGATG 6 cut(s) 9, 503, 583, 634, 690, 1303
BseLI CCNNNNNNNGG 3 cut(s) 700, 1256, 1834
BseMI GCAATG 2 cut(s) 438, 628
BseMII CTCAG 8 cut(s) 167, 585, 723, 795, 1257, 1641, 1737, 1751
BseNI ACTGG 4 cut(s) 184, 1116, 1408, 1414
BseRI GAGGAG 4 cut(s) 74, 164, 214, 217
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 5 cut(s) 432, 1192, 1195, 1369, 1715
BseYI CCCAGC 2 cut(s) 638, 767
Bsh1236I CGCG 1 cut(s) 1851
BshFI GGCC 4 cut(s) 5, 98, 143, 320
BshNI GGYRCC 1 cut(s) 1391
BshTI ACCGGT 1 cut(s) 475
BshVI ATCGAT 2 cut(s) 525, 1141
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 2 cut(s) 93, 1568
BsiSI CCGG 5 cut(s) 94, 140, 476, 1746, 1835
BslFI GGGAC 2 cut(s) 76, 154
BslI CCNNNNNNNGG 3 cut(s) 700, 1256, 1834
BsmAI GTCTC 5 cut(s) 139, 1049, 1294, 1657, 1740
BsmBI CGTCTC 2 cut(s) 139, 1657
BsmFI GGGAC 2 cut(s) 76, 154
BsnI GGCC 4 cut(s) 5, 98, 143, 320
BsoBI CYCGRG 2 cut(s) 93, 1568
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 100, 312
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
BspACI CCGC 8 cut(s) 149, 240, 243, 280, 292, 344, 414, 833
BspANI GGCC 4 cut(s) 5, 98, 143, 320
BspCNI CTCAG 8 cut(s) 166, 586, 722, 796, 1256, 1640, 1736, 1750
BspDI ATCGAT 2 cut(s) 525, 1141
BspFNI CGCG 1 cut(s) 1851
BspHI TCATGA 2 cut(s) 727, 1056
BspLI GGNNCC 7 cut(s) 77, 98, 253, 290, 1116, 1393, 1449
BspMI ACCTGC 1 cut(s) 1648
BspPI GGATC 6 cut(s) 660, 751, 1355, 1442, 1455, 1466
BspT107I GGYRCC 1 cut(s) 1391
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 438, 628
BsrFI RCCGGY 1 cut(s) 475
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 4 cut(s) 184, 1116, 1408, 1414
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 93, 138
BssMI GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
BssNI GRCGYC 1 cut(s) 1392
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 3 cut(s) 1351, 1585, 1772
BstACI GRCGYC 1 cut(s) 1392
BstAUI TGTACA 1 cut(s) 915
BstBAI YACGTR 1 cut(s) 1761
BstC8I GCNNGC 3 cut(s) 564, 765, 1171
BstEII GGTNACC 1 cut(s) 894
BstF5I GGATG 6 cut(s) 9, 503, 583, 634, 690, 1303
BstFNI CGCG 1 cut(s) 1851
BstH2I RGCGCY 2 cut(s) 1395, 1794
BstHHI GCGC 2 cut(s) 1394, 1793
BstKTI GATC 8 cut(s) 655, 727, 746, 1001, 1145, 1363, 1450, 1474
BstMAI GTCTC 5 cut(s) 139, 1049, 1294, 1657, 1740
BstMBI GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
BstMWI GCNNNNNNNGC 7 cut(s) 343, 1202, 1214, 1391, 1398, 1688, 1857
BstNI CCWGG 1 cut(s) 286
BstPAI GACNNNNGTC 1 cut(s) 1830
BstPI GGTNACC 1 cut(s) 894
BstSCI CCNGG 5 cut(s) 92, 93, 138, 284, 1744
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 100
BstUI CGCG 1 cut(s) 1851
BstV1I GCAGC 5 cut(s) 432, 1192, 1195, 1369, 1715
BstV2I GAAGAC 1 cut(s) 1773
BstX2I RGATCY 1 cut(s) 1447
BstYI RGATCY 1 cut(s) 1447
Bsu15I ATCGAT 2 cut(s) 525, 1141
BsuRI GGCC 4 cut(s) 5, 98, 143, 320
BsuTUI ATCGAT 2 cut(s) 525, 1141
BtsCI GGATG 6 cut(s) 9, 503, 583, 634, 690, 1303
BtsIMutI CAGTG 2 cut(s) 1401, 1599
BveI ACCTGC 1 cut(s) 1648
Cac8I GCNNGC 3 cut(s) 564, 765, 1171
CciI TCATGA 2 cut(s) 727, 1056
CfoI GCGC 2 cut(s) 1394, 1793
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 3 cut(s) 96, 97, 319
Cfr9I CCCGGG 1 cut(s) 93
ClaI ATCGAT 2 cut(s) 525, 1141
CseI GACGC 1 cut(s) 1840
Csp6I GTAC 3 cut(s) 436, 916, 1078
CspAI ACCGGT 1 cut(s) 475
CviAII CATG 9 cut(s) 257, 649, 728, 876, 1030, 1057, 1067, 1444, 1492
CviQI GTAC 3 cut(s) 436, 916, 1078
DinI GGCGCC 1 cut(s) 1393
DpnI GATC 8 cut(s) 654, 726, 745, 1000, 1144, 1362, 1449, 1473
DpnII GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
DriI GACNNNNNGTC 2 cut(s) 1732, 1850
Eam1105I GACNNNNNGTC 2 cut(s) 1732, 1850
EciI GGCGGA 2 cut(s) 229, 429
Ecl136II GAGCTC 1 cut(s) 310
Eco24I GRGCYC 2 cut(s) 100, 312
Eco32I GATATC 1 cut(s) 684
Eco47III AGCGCT 1 cut(s) 1792
Eco53kI GAGCTC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 1761
Eco88I CYCGRG 2 cut(s) 93, 1568
Eco91I GGTNACC 1 cut(s) 894
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 894
EcoRII CCWGG 1 cut(s) 284
EcoRV GATATC 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 1108
EcoT38I GRGCYC 2 cut(s) 100, 312
EgeI GGCGCC 1 cut(s) 1393
EheI GGCGCC 1 cut(s) 1393
Esp3I CGTCTC 2 cut(s) 139, 1657
FaeI CATG 9 cut(s) 260, 652, 731, 879, 1033, 1060, 1070, 1447, 1495
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 2 cut(s) 76, 154
FatI CATG 9 cut(s) 256, 648, 727, 875, 1029, 1056, 1066, 1443, 1491
FauNDI CATATG 2 cut(s) 1003, 1636
FbaI TGATCA 1 cut(s) 724
Fnu4HI GCNGC 8 cut(s) 149, 243, 344, 446, 1206, 1209, 1383, 1704
FokI GGATG 5 cut(s) 490, 590, 621, 677, 1290
FriOI GRGCYC 2 cut(s) 100, 312
Fsp4HI GCNGC 8 cut(s) 149, 243, 344, 446, 1206, 1209, 1383, 1704
FspBI CTAG 2 cut(s) 71, 1688
GlaI GCGC 2 cut(s) 1393, 1792
GluI GCNGC 8 cut(s) 149, 243, 344, 446, 1206, 1209, 1383, 1704
GsaI CCCAGC 2 cut(s) 642, 771
GsuI CTGGAG 2 cut(s) 167, 1278
HaeII RGCGCY 2 cut(s) 1395, 1794
HaeIII GGCC 4 cut(s) 5, 98, 143, 320
HapII CCGG 5 cut(s) 94, 140, 476, 1746, 1835
HgaI GACGC 1 cut(s) 1840
HhaI GCGC 2 cut(s) 1394, 1793
Hin1I GRCGYC 1 cut(s) 1392
Hin1II CATG 9 cut(s) 260, 652, 731, 879, 1033, 1060, 1070, 1447, 1495
Hin6I GCGC 2 cut(s) 1392, 1791
HinP1I GCGC 2 cut(s) 1392, 1791
HindIII AAGCTT 3 cut(s) 50, 119, 560
HinfI GANTC 5 cut(s) 167, 670, 1327, 1727, 1733
HpaII CCGG 5 cut(s) 94, 140, 476, 1746, 1835
HphI GGTGA 6 cut(s) 761, 1173, 1336, 1355, 1520, 1672
Hpy166II GTNNAC 3 cut(s) 463, 791, 916
Hpy188I TCNGA 8 cut(s) 58, 352, 407, 595, 1589, 1710, 1726, 1732
Hpy188III TCNNGA 8 cut(s) 209, 575, 701, 728, 1057, 1257, 1364, 1570
Hpy8I GTNNAC 3 cut(s) 463, 791, 916
Hpy99I CGWCG 4 cut(s) 164, 167, 308, 329
HpyAV CCTTC 8 cut(s) 199, 215, 246, 752, 772, 808, 1317, 1662
HpyCH4III ACNGT 3 cut(s) 1351, 1585, 1772
HpyCH4IV ACGT 3 cut(s) 606, 1760, 1822
HpyCH4V TGCA 4 cut(s) 431, 581, 1106, 1196
HpyF10VI GCNNNNNNNGC 7 cut(s) 343, 1202, 1214, 1391, 1398, 1688, 1857
HpySE526I ACGT 3 cut(s) 606, 1760, 1822
Hsp92I GRCGYC 1 cut(s) 1392
Hsp92II CATG 9 cut(s) 260, 652, 731, 879, 1033, 1060, 1070, 1447, 1495
HspAI GCGC 2 cut(s) 1392, 1791
KasI GGCGCC 1 cut(s) 1391
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
LmnI GCTCC 8 cut(s) 87, 257, 294, 299, 631, 1259, 1687, 1711
Lsp1109I GCAGC 5 cut(s) 432, 1192, 1195, 1369, 1715
LweI GCATC 4 cut(s) 568, 643, 1147, 1156
MaeI CTAG 2 cut(s) 71, 1688
MaeII ACGT 3 cut(s) 606, 1760, 1822
MaeIII GTNAC 5 cut(s) 894, 983, 1526, 1563, 1823
MalI GATC 8 cut(s) 654, 726, 745, 1000, 1144, 1362, 1449, 1473
MbiI CCGCTC 1 cut(s) 294
MboI GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
MboII GAAGA 6 cut(s) 622, 784, 1181, 1283, 1628, 1778
MfeI CAATTG 2 cut(s) 906, 1315
MflI RGATCY 1 cut(s) 1447
MhlI GDGCHC 2 cut(s) 100, 312
MluCI AATT 8 cut(s) 481, 570, 660, 857, 863, 906, 1223, 1315
MluI ACGCGT 1 cut(s) 1849
Mly113I GGCGCC 1 cut(s) 1392
MlyI GAGTC 3 cut(s) 176, 1721, 1742
MmeI TCCRAC 4 cut(s) 81, 357, 1730, 1733
Mph1103I ATGCAT 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 1411
MseI TTAA 3 cut(s) 480, 992, 1175
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 5 cut(s) 94, 140, 476, 1746, 1835
MspR9I CCNGG 5 cut(s) 94, 95, 140, 286, 1746
MunI CAATTG 2 cut(s) 906, 1315
MvaI CCWGG 1 cut(s) 286
MvnI CGCG 1 cut(s) 1851
MwoI GCNNNNNNNGC 7 cut(s) 343, 1202, 1214, 1391, 1398, 1688, 1857
NarI GGCGCC 1 cut(s) 1392
NciI CCSGG 4 cut(s) 94, 95, 140, 1746
NdeI CATATG 2 cut(s) 1003, 1636
NdeII GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
NlaIII CATG 9 cut(s) 260, 652, 731, 879, 1033, 1060, 1070, 1447, 1495
NlaIV GGNNCC 7 cut(s) 77, 98, 253, 290, 1116, 1393, 1449
NmuCI GTSAC 4 cut(s) 983, 1526, 1563, 1823
NsiI ATGCAT 1 cut(s) 1108
PaeR7I CTCGAG 1 cut(s) 1568
PagI TCATGA 2 cut(s) 727, 1056
PaqCI CACCTGC 1 cut(s) 1648
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 1411
PfeI GAWTC 2 cut(s) 670, 1327
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 8 cut(s) 150, 244, 345, 447, 1207, 1210, 1384, 1705
PleI GAGTC 3 cut(s) 175, 1721, 1741
PluTI GGCGCC 1 cut(s) 1395
PmaCI CACGTG 1 cut(s) 1761
PmlI CACGTG 1 cut(s) 1761
PpsI GAGTC 3 cut(s) 175, 1721, 1741
Ppu21I YACGTR 1 cut(s) 1761
PshAI GACNNNNGTC 1 cut(s) 1830
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 284
PspCI CACGTG 1 cut(s) 1761
PspEI GGTNACC 1 cut(s) 894
PspFI CCCAGC 2 cut(s) 638, 767
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 7 cut(s) 77, 98, 253, 290, 1116, 1393, 1449
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 319
PsuI RGATCY 1 cut(s) 1447
RsaI GTAC 3 cut(s) 437, 917, 1079
RsaNI GTAC 3 cut(s) 436, 916, 1078
RseI CAYNNNNRTG 1 cut(s) 386
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 3 cut(s) 480, 992, 1175
SatI GCNGC 8 cut(s) 149, 243, 344, 446, 1206, 1209, 1383, 1704
Sau3AI GATC 8 cut(s) 652, 724, 743, 998, 1142, 1360, 1447, 1471
Sau96I GGNCC 3 cut(s) 96, 97, 319
SchI GAGTC 3 cut(s) 176, 1721, 1742
ScrFI CCNGG 5 cut(s) 94, 95, 140, 286, 1746
SduI GDGCHC 2 cut(s) 100, 312
SfaNI GCATC 4 cut(s) 568, 643, 1147, 1156
SfcI CTRYAG 1 cut(s) 468
SfoI GGCGCC 1 cut(s) 1393
Sfr274I CTCGAG 1 cut(s) 1568
SlaI CTCGAG 1 cut(s) 1568
SmaI CCCGGG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 2 cut(s) 362, 1568
SmoI CTYRAG 2 cut(s) 362, 1568
Sse9I AATT 8 cut(s) 481, 570, 660, 857, 863, 906, 1223, 1315
SsiI CCGC 8 cut(s) 149, 240, 243, 280, 292, 344, 414, 833
SspDI GGCGCC 1 cut(s) 1391
SspI AATATT 2 cut(s) 841, 1647
SspMI CTAG 2 cut(s) 71, 1688
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 5 cut(s) 92, 93, 138, 284, 1744
TaaI ACNGT 3 cut(s) 1351, 1585, 1772
TaiI ACGT 3 cut(s) 609, 1763, 1825
TaqI TCGA 7 cut(s) 165, 303, 327, 525, 997, 1141, 1569
TasI AATT 8 cut(s) 481, 570, 660, 857, 863, 906, 1223, 1315
TatI WGTACW 1 cut(s) 915
TauI GCSGC 3 cut(s) 151, 245, 346
TfiI GAWTC 2 cut(s) 670, 1327
Tru1I TTAA 3 cut(s) 480, 992, 1175
Tru9I TTAA 3 cut(s) 480, 992, 1175
TscAI CASTG 2 cut(s) 1408, 1606
TseFI GTSAC 4 cut(s) 983, 1526, 1563, 1823
TseI GCWGC 5 cut(s) 445, 1205, 1208, 1382, 1703
Tsp45I GTSAC 4 cut(s) 983, 1526, 1563, 1823
TspDTI ATGAA 9 cut(s) 262, 662, 771, 791, 869, 1274, 1476, 1493, 1623
TspGWI ACGGA 4 cut(s) 521, 1242, 1312, 1514
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 2 cut(s) 1408, 1606
XapI RAATTY 2 cut(s) 570, 863
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 1568
XmaI CCCGGG 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 1411
XspI CTAG 2 cut(s) 71, 1688
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.