pycom10g13320

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003194
Physical Location & Seq
Forward (+)
16760319 .. 16762065
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGTTGGGCCTAAGCTTCTCTCACAGCCTAAAAACAACAATAATCTTACGGTGCGTACCTGATGCAGCGGATTACAACTTTTCCATATATATTCCCTCACTTAAAACCCTAGTTCTATCCGTGCCTCTCCCAGGACTCCAAAGCACCATACTACTCTTCGTCTCAGTCAGGGCCGTCCCTTTGCTGAAGAAGCAGATTGTGAAGAAGCGCAAGACGCACTTCAAGAGGCCCCAGAGTGACAGGAAGCACTGCGTCAAGGGTATTGATTCACGTGTCAGGCGAAAGTTCAAGGGATGTGCATTGATGCCCAACATTGGCTATGGGTCAGACAAGAAGACTCGTCACTATCTGCCTAACAAGTTCAAGAAATTTGTCGTGCACAATGTCAAAGAGGTTGAACTTCTAATGATGCACAACAGGACTTACTGTGCTGAGATTGCACACAACATTTCCACCAGGAAGAGGAAGGAGATCGTCGAGCGTGCAGCCCAGTTGGATGTTGTTGTTACCAACAAACTTGCCAGGTTGCGTAGCCAGGAGGACGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001889 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009410 GO:0009719 GO:0009725 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0014070 GO:0015031 GO:0015833 GO:0015934 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0019538 GO:0022625 GO:0022626 GO:0030867 GO:0031090 GO:0031099 GO:0031100 GO:0031960 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033365 GO:0033993 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0035690 GO:0042175 GO:0042221 GO:0042493 GO:0042788 GO:0042886 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048513 GO:0048519 GO:0048545 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0051384 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061008 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070972 GO:0071310 GO:0071383 GO:0071384 GO:0071385 GO:0071396 GO:0071407 GO:0071466 GO:0071495 GO:0071548 GO:0071549 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:0097327 GO:0097421 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901654 GO:1901655 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 ko03022 ko04080 ko04141 ko04725 map03010 map03022 map04080 map04141 map04725
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

183

Amino Acids

20.94

Weight (kDa)

10.27

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 72 - 171 4.9e-37 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 68
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 206
AcvI CACGTG 1 cut(s) 272
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 3 cut(s) 131, 314, 462
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 4 cut(s) 223, 289, 364, 398
AjnI CCWGG 4 cut(s) 130, 455, 521, 534
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 166
Alw44I GTGCAC 1 cut(s) 377
AoxI GGCC 3 cut(s) 7, 171, 227
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 2 cut(s) 65, 485
ApoI RAATTY 1 cut(s) 368
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 3 cut(s) 7, 171, 228
BaeGI GKGCMC 1 cut(s) 381
BbrPI CACGTG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 77, 497
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 4 cut(s) 132, 457, 523, 536
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 1 cut(s) 110
BisI GCNGC 2 cut(s) 66, 486
BlsI GCNGC 2 cut(s) 67, 487
Bme1390I CCNGG 4 cut(s) 132, 457, 523, 536
BmgT120I GGNCC 3 cut(s) 7, 171, 228
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 4 cut(s) 132, 457, 523, 536
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 3 cut(s) 52, 294, 399
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 130
BsaXI ACNNNNNCTCC 1 cut(s) 530
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 314, 462
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 4 cut(s) 132, 457, 523, 536
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 299, 502
BseLI CCNNNNNNNGG 3 cut(s) 131, 314, 462
BseMII CTCAG 2 cut(s) 177, 423
BseNI ACTGG 1 cut(s) 490
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 2 cut(s) 77, 497
BsgI GTGCAG 1 cut(s) 504
BshFI GGCC 3 cut(s) 9, 173, 229
BsiHKAI GWGCWC 1 cut(s) 381
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 131, 314, 462
BsmAI GTCTC 1 cut(s) 166
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 3 cut(s) 9, 173, 229
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 1 cut(s) 471
BspACI CCGC 1 cut(s) 68
BspANI GGCC 3 cut(s) 9, 173, 229
BspCNI CTCAG 2 cut(s) 176, 424
BspLI GGNNCC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 471
Bst2UI CCWGG 4 cut(s) 132, 457, 523, 536
Bst4CI ACNGT 2 cut(s) 51, 428
Bst6I CTCTTC 2 cut(s) 161, 455
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 3 cut(s) 11, 163, 432
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 299, 502
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 1 cut(s) 474
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 1 cut(s) 471
BstMWI GCNNNNNNNGC 3 cut(s) 190, 214, 437
BstNI CCWGG 4 cut(s) 132, 457, 523, 536
BstSCI CCNGG 4 cut(s) 130, 455, 521, 534
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 77, 497
BstV2I GAAGAC 1 cut(s) 341
BsuRI GGCC 3 cut(s) 9, 173, 229
BtsCI GGATG 2 cut(s) 299, 502
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 7, 171, 228
CseI GACGC 2 cut(s) 223, 241
Csp6I GTAC 1 cut(s) 56
CviJI RGCY 8 cut(s) 9, 15, 27, 173, 229, 318, 488, 534
CviKI_1 RGCY 8 cut(s) 9, 15, 27, 173, 229, 318, 488, 534
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 3 cut(s) 11, 163, 432
DpnI GATC 1 cut(s) 473
DpnII GATC 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 161, 455
EarI CTCTTC 2 cut(s) 161, 455
Eco57I CTGAAG 1 cut(s) 206
Eco72I CACGTG 1 cut(s) 272
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 228
EcoRII CCWGG 4 cut(s) 130, 455, 521, 534
Esp3I CGTCTC 1 cut(s) 166
FaiI YATR 5 cut(s) 86, 88, 90, 149, 321
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 66, 486
FokI GGATG 2 cut(s) 306, 509
Fsp4HI GCNGC 2 cut(s) 66, 486
FspBI CTAG 1 cut(s) 110
GlaI GCGC 1 cut(s) 209
GluI GCNGC 2 cut(s) 66, 486
HaeIII GGCC 3 cut(s) 9, 173, 229
HgaI GACGC 2 cut(s) 223, 241
HhaI GCGC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 3 cut(s) 135, 266, 337
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 2 cut(s) 223, 364
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 2 cut(s) 51, 428
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 6 cut(s) 65, 299, 379, 412, 440, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 214, 437
HpyF3I CTNAG 3 cut(s) 11, 163, 432
HpySE526I ACGT 1 cut(s) 271
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 1 cut(s) 471
Lsp1109I GCAGC 2 cut(s) 77, 497
LweI GCATC 3 cut(s) 52, 294, 399
MaeI CTAG 1 cut(s) 110
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 3 cut(s) 236, 341, 505
MalI GATC 1 cut(s) 473
MboI GATC 1 cut(s) 471
MboII GAAGA 5 cut(s) 148, 199, 214, 346, 472
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 1 cut(s) 368
MlyI GAGTC 2 cut(s) 129, 331
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 6 cut(s) 106, 135, 219, 385, 456, 532
MseI TTAA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 4 cut(s) 132, 457, 523, 536
MvaI CCWGG 4 cut(s) 132, 457, 523, 536
MwoI GCNNNNNNNGC 3 cut(s) 190, 214, 437
NdeII GATC 1 cut(s) 471
NlaIV GGNNCC 1 cut(s) 230
NmuCI GTSAC 2 cut(s) 236, 341
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 2 cut(s) 67, 487
PleI GAGTC 2 cut(s) 129, 331
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
PpsI GAGTC 2 cut(s) 129, 331
Ppu21I YACGTR 1 cut(s) 272
Psp6I CCWGG 4 cut(s) 130, 455, 521, 534
PspCI CACGTG 1 cut(s) 272
PspGI CCWGG 4 cut(s) 130, 455, 521, 534
PspN4I GGNNCC 1 cut(s) 230
PspPI GGNCC 3 cut(s) 7, 171, 228
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 66, 486
Sau3AI GATC 1 cut(s) 471
Sau96I GGNCC 3 cut(s) 7, 171, 228
SchI GAGTC 2 cut(s) 129, 331
ScrFI CCNGG 4 cut(s) 132, 457, 523, 536
SduI GDGCHC 1 cut(s) 381
SetI ASST 5 cut(s) 17, 61, 274, 396, 527
SfaNI GCATC 3 cut(s) 52, 294, 399
Sse9I AATT 1 cut(s) 368
SsiI CCGC 1 cut(s) 68
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 4 cut(s) 130, 455, 521, 534
TaaI ACNGT 2 cut(s) 51, 428
TaiI ACGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 477
TasI AATT 1 cut(s) 368
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 2 cut(s) 236, 341
TseI GCWGC 2 cut(s) 65, 485
Tsp45I GTSAC 2 cut(s) 236, 341
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 1 cut(s) 254
VneI GTGCAC 1 cut(s) 377
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 1 cut(s) 368
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.