pycom10g17430

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004498
Physical Location & Seq
Forward (+)
20671656 .. 20676473
4818 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGAAGGAGACTGGTAGAAAACAAGGTGCTATGTCTCCATGCGCAGCATGCAAACTTCTGCGAAGGAGATGCGCTCAGGACTGTGTTTTTGCTCCTTATTTTCCAGCTGACGAGCCCCAGAAGTTTGCCAGTGTCCACAAGGTTTTTGGTGCTAGCAATGTCAACAAGATGCTACAGGAATTACCAGAGCACCAACGCAGTGATGCAGTGAGTTCCATGGTGTATGAAGCAAATGCAAGAGTCCGGGATCCAGTGTACGGTTGTGTTGGAGCAATATCATCTTTACAACAACAAATCGACGCCCTCCAAACCCAATTGGCCATTGCACAAGCTGAGGTGGTGCACATGAGAATGGGCCAATTCCCTACTACCACTACTATTGCTTCAACGACTACTTGCTCACCAGACCAAAAGATGGCACCTGATAATTCTCATCATCATCGTAACCATCAAACGGCTACCACCAAGTCCTTTTTTTCTATGGACATAGTAGATCAGGCCAATATGGGAGAGTCTTTGTGGTCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

177

Amino Acids

19.44

Weight (kDa)

7.64

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 13 - 110 8.2e-44 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 415
AclWI GGATC 2 cut(s) 242, 255
AcoI YGGCCR 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 257, 415, 454
AgsI TTSAA 1 cut(s) 387
AluBI AGCT 2 cut(s) 107, 332
AluI AGCT 2 cut(s) 107, 332
Alw21I GWGCWC 2 cut(s) 192, 345
Alw26I GTCTC 2 cut(s) 2, 39
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 2 cut(s) 242, 255
AoxI GGCC 3 cut(s) 318, 355, 498
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AspLEI GCGC 2 cut(s) 44, 74
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 393
AsuNHI GCTAGC 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 320
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 192, 345
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 409, 456
BceAI ACGGC 1 cut(s) 471
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 2, 39
BfaI CTAG 2 cut(s) 153, 529
BfmI CTRYAG 1 cut(s) 173
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 2 cut(s) 249, 420
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 59, 159, 193
BmtI GCTAGC 1 cut(s) 156
BplI GAGNNNNNCTC 2 cut(s) 58, 90
Bpu10I CCTNAGC 2 cut(s) 75, 333
BpuMI CCSGG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 415, 454
Bse1I ACTGG 3 cut(s) 16, 129, 251
Bse3DI GCAATG 2 cut(s) 163, 321
BseDI CCNNGG 1 cut(s) 216
BseLI CCNNNNNNNGG 3 cut(s) 257, 415, 454
BseMI GCAATG 2 cut(s) 163, 321
BseMII CTCAG 2 cut(s) 89, 324
BseNI ACTGG 3 cut(s) 16, 129, 251
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 1 cut(s) 56
BshFI GGCC 3 cut(s) 320, 357, 500
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 192, 345
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 3 cut(s) 257, 415, 454
BsmAI GTCTC 2 cut(s) 2, 39
BsnI GGCC 3 cut(s) 320, 357, 500
Bsp1286I GDGCHC 3 cut(s) 117, 192, 345
Bsp143I GATC 2 cut(s) 247, 493
Bsp19I CCATGG 1 cut(s) 216
BspANI GGCC 3 cut(s) 320, 357, 500
BspCNI CTCAG 2 cut(s) 88, 325
BspLI GGNNCC 2 cut(s) 249, 420
BspOI GCTAGC 1 cut(s) 156
BspPI GGATC 2 cut(s) 242, 255
BspT107I GGYRCC 1 cut(s) 418
BsrDI GCAATG 2 cut(s) 163, 321
BsrI ACTGG 3 cut(s) 16, 129, 251
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 2 cut(s) 247, 493
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 2 cut(s) 83, 260
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 49, 154
BstDEI CTNAG 2 cut(s) 75, 333
BstDSI CCRYGG 1 cut(s) 216
BstHHI GCGC 2 cut(s) 44, 74
BstKTI GATC 2 cut(s) 250, 496
BstMAI GTCTC 2 cut(s) 2, 39
BstMBI GATC 2 cut(s) 247, 493
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 3 cut(s) 320, 357, 500
BtgI CCRYGG 1 cut(s) 216
BtsI GCAGTG 2 cut(s) 205, 213
BtsIMutI CAGTG 4 cut(s) 136, 205, 213, 258
Cac8I GCNNGC 2 cut(s) 49, 154
CfoI GCGC 2 cut(s) 44, 74
Cfr13I GGNCC 1 cut(s) 355
CseI GACGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 5 cut(s) 39, 48, 217, 346, 525
CviJI RGCY 7 cut(s) 107, 115, 320, 332, 357, 458, 500
CviKI_1 RGCY 7 cut(s) 107, 115, 320, 332, 357, 458, 500
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 2 cut(s) 75, 333
DpnI GATC 2 cut(s) 249, 495
DpnII GATC 2 cut(s) 247, 493
EaeI YGGCCR 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 5 cut(s) 42, 51, 220, 349, 528
FatI CATG 5 cut(s) 38, 47, 216, 345, 524
Fnu4HI GCNGC 1 cut(s) 45
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 2 cut(s) 153, 529
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 2 cut(s) 43, 73
GluI GCNGC 1 cut(s) 45
HaeIII GGCC 3 cut(s) 320, 357, 500
HapII CCGG 1 cut(s) 244
HgaI GACGC 1 cut(s) 308
HhaI GCGC 2 cut(s) 44, 74
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 5 cut(s) 42, 51, 220, 349, 528
Hin6I GCGC 2 cut(s) 42, 72
HinP1I GCGC 2 cut(s) 42, 72
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 240, 512
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 4 cut(s) 136, 163, 256, 343
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 4 cut(s) 136, 163, 256, 343
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 83, 260
HpyCH4V TGCA 5 cut(s) 51, 206, 236, 326, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpyF3I CTNAG 2 cut(s) 75, 333
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 5 cut(s) 42, 51, 220, 349, 528
HspAI GCGC 2 cut(s) 42, 72
Kzo9I GATC 2 cut(s) 247, 493
LmnI GCTCC 2 cut(s) 97, 269
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 3 cut(s) 59, 159, 193
MaeI CTAG 2 cut(s) 153, 529
MaeIII GTNAC 1 cut(s) 443
MalI GATC 2 cut(s) 249, 495
MboI GATC 2 cut(s) 247, 493
MfeI CAATTG 1 cut(s) 314
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 3 cut(s) 117, 192, 345
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 4 cut(s) 179, 314, 359, 427
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 2 cut(s) 249, 521
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 2 cut(s) 314, 328
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MslI CAYNNNNRTG 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 1 cut(s) 216
NdeII GATC 2 cut(s) 247, 493
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 5 cut(s) 42, 51, 220, 349, 528
NlaIV GGNNCC 2 cut(s) 249, 420
NsbI TGCGCA 1 cut(s) 43
NspI RCATGY 1 cut(s) 51
PaeI GCATGC 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 2 cut(s) 248, 520
PpsI GAGTC 2 cut(s) 248, 520
PspN4I GGNNCC 2 cut(s) 249, 420
PspPI GGNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 350
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 2 cut(s) 247, 493
Sau96I GGNCC 1 cut(s) 355
SchI GAGTC 2 cut(s) 249, 521
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 3 cut(s) 117, 192, 345
SetI ASST 6 cut(s) 28, 109, 144, 334, 339, 424
SfaNI GCATC 3 cut(s) 59, 159, 193
SfcI CTRYAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 350
SphI GCATGC 1 cut(s) 51
Sse9I AATT 4 cut(s) 179, 314, 359, 427
SspMI CTAG 2 cut(s) 153, 529
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 83, 260
TaqI TCGA 1 cut(s) 297
TasI AATT 4 cut(s) 179, 314, 359, 427
TscAI CASTG 4 cut(s) 136, 205, 213, 258
TseI GCWGC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 17, 240
TspRI CASTG 4 cut(s) 136, 205, 213, 258
Van91I CCANNNNNTGG 1 cut(s) 415
VneI GTGCAC 1 cut(s) 341
XceI RCATGY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XspI CTAG 2 cut(s) 153, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.