pycom10g24060

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004799
Physical Location & Seq
Forward (+)
25962115 .. 25963084
970 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGAGTTTTGAAAAGGAGGACCTTGATCTGGTTTTGGTCCCAAGTGGATTGCTCATCATGTTTGTCTATCACATCATCCTTCTCTACAGATATATCCATCTCCCTCATACCACAGTTCTTGGCTTTGAAAACAATGACAAAAGAGCTTGGGCTGAAAGAATCATGCAGGTTGATAAAAGAGACGTAGGCACAGCCCTAAGTGTGATTTCATCTAACACATCAGCAGCAACTTTTCTGTGTTCCATCTCCTTAACTCTCAGCTCCCTCATCGGAGCTTGGATCGGAAGTAATTCGAGTCAGACAGTCTTCCAGAGTGAATTAATTTATGGAAGCACCGACCCATCAATCCTCACAATGAAATACATAAGCCTCTTAACTTGTTTCCTTCTAGCCTTCGCATGCTTTGTTCAATCAGCAAGGCACTTTGTCCATGCAAACTATCTGATCAGCATGCCGGACAGTAACATTCCGGCGTGGTACGTGCAGATGGCGGTCATAAGGGGAGGTGATTTCTGGTCACTTGGGCTTAGAGCACTCTATTTTGCTCTAACTCTTTTGCTATGGTTTTTCGGTCCGATTCCAATGTTTATTTCCTCCATGGTTTTGGTCATAGTCCTCCATTACCTTGACACAAACACCAGGCCACTGCACCAGCACCAGCTTCCCGGAAAGGAGATCGTCAAACGCGTCGGCGAAAGGATCTCAGAGGTGGCTGTGACCATTCAGCGTCATACAGAAAGGGTTGAAACTACTAGAAGTACCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

256

Amino Acids

28.81

Weight (kDa)

7.12

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 2.6e-61 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AccII CGCG 1 cut(s) 687
AciI CCGC 2 cut(s) 491, 762
AclWI GGATC 2 cut(s) 287, 707
AfaI GTAC 2 cut(s) 479, 760
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 4 cut(s) 11, 128, 410, 746
AjnI CCWGG 1 cut(s) 638
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AluBI AGCT 4 cut(s) 146, 261, 275, 661
AluI AGCT 4 cut(s) 146, 261, 275, 661
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 2 cut(s) 287, 707
AoxI GGCC 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 224
AseI ATTAAT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 19, 37, 572
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 3 cut(s) 19, 37, 572
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 236
BccI CCATC 4 cut(s) 105, 251, 349, 481
BciT130I CCWGG 1 cut(s) 640
BclI TGATCA 1 cut(s) 444
BcnI CCSGG 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 389, 753
BfmI CTRYAG 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 640, 666
Bme18I GGWCC 3 cut(s) 19, 37, 572
BmgT120I GGNCC 3 cut(s) 19, 37, 572
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 640, 666
BpiI GAAGAC 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 597
Bsc4I CCNNNNNNNGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 640
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 271, 717
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 2 cut(s) 503, 632
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 3 cut(s) 455, 470, 666
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 643
Bsp1286I GDGCHC 1 cut(s) 535
Bsp143I GATC 5 cut(s) 25, 279, 444, 675, 699
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 2 cut(s) 491, 762
BspANI GGCC 1 cut(s) 643
BspCNI CTCAG 2 cut(s) 270, 716
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 2 cut(s) 287, 707
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 5 cut(s) 25, 279, 444, 675, 699
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 3 cut(s) 115, 304, 461
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 400, 452
BstDEI CTNAG 4 cut(s) 197, 257, 527, 703
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 687
BstKTI GATC 5 cut(s) 28, 282, 447, 678, 702
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 5 cut(s) 25, 279, 444, 675, 699
BstNI CCWGG 1 cut(s) 640
BstNSI RCATGY 2 cut(s) 402, 454
BstSCI CCNGG 2 cut(s) 638, 664
BstSFI CTRYAG 1 cut(s) 85
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 699
BsuRI GGCC 1 cut(s) 643
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 1 cut(s) 644
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 400, 452
Cfr13I GGNCC 3 cut(s) 19, 37, 572
CpoI CGGWCCG 1 cut(s) 572
CseI GACGC 2 cut(s) 676, 716
Csp6I GTAC 2 cut(s) 478, 759
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CspI CGGWCCG 1 cut(s) 572
CviAII CATG 6 cut(s) 58, 163, 399, 431, 451, 598
CviQI GTAC 2 cut(s) 478, 759
DdeI CTNAG 4 cut(s) 197, 257, 527, 703
DpnI GATC 5 cut(s) 27, 281, 446, 677, 701
DpnII GATC 5 cut(s) 25, 279, 444, 675, 699
Eco130I CCWWGG 1 cut(s) 597
Eco47I GGWCC 3 cut(s) 19, 37, 572
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 597
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 61, 166, 402, 434, 454, 601
FaqI GGGAC 1 cut(s) 23
FatI CATG 6 cut(s) 57, 162, 398, 430, 450, 597
FbaI TGATCA 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 389, 753
GluI GCNGC 1 cut(s) 225
HaeIII GGCC 1 cut(s) 643
HapII CCGG 3 cut(s) 455, 470, 666
HgaI GACGC 2 cut(s) 676, 716
Hin1II CATG 6 cut(s) 61, 166, 402, 434, 454, 601
HinfI GANTC 3 cut(s) 159, 295, 577
HpaII CCGG 3 cut(s) 455, 470, 666
HphI GGTGA 1 cut(s) 518
Hpy188I TCNGA 6 cut(s) 272, 284, 300, 444, 576, 706
Hpy188III TCNNGA 1 cut(s) 310
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 89, 395, 403
HpyCH4III ACNGT 3 cut(s) 115, 304, 461
HpyCH4IV ACGT 2 cut(s) 183, 480
HpyCH4V TGCA 4 cut(s) 166, 434, 484, 649
HpyF3I CTNAG 4 cut(s) 197, 257, 527, 703
HpySE526I ACGT 2 cut(s) 183, 480
Hsp92II CATG 6 cut(s) 61, 166, 402, 434, 454, 601
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 25, 279, 444, 675, 699
LmnI GCTCC 2 cut(s) 266, 272
Lsp1109I GCAGC 1 cut(s) 236
MaeI CTAG 2 cut(s) 389, 753
MaeII ACGT 2 cut(s) 183, 480
MaeIII GTNAC 3 cut(s) 461, 516, 715
MalI GATC 5 cut(s) 27, 281, 446, 677, 701
MboI GATC 5 cut(s) 25, 279, 444, 675, 699
MboII GAAGA 1 cut(s) 298
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 3 cut(s) 289, 317, 321
MluI ACGCGT 1 cut(s) 685
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 9 cut(s) 10, 114, 275, 359, 380, 497, 604, 626, 700
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 3 cut(s) 251, 320, 374
MspI CCGG 3 cut(s) 455, 470, 666
MspR9I CCNGG 2 cut(s) 640, 666
MvaI CCWGG 1 cut(s) 640
MvnI CGCG 1 cut(s) 687
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 597
NdeII GATC 5 cut(s) 25, 279, 444, 675, 699
NlaIII CATG 6 cut(s) 61, 166, 402, 434, 454, 601
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 516, 715
NspI RCATGY 2 cut(s) 402, 454
PaeI GCATGC 2 cut(s) 402, 454
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 159, 577
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 19, 37, 572
PspPPI RGGWCCY 1 cut(s) 19
PsuI RGATCY 1 cut(s) 699
RsaI GTAC 2 cut(s) 479, 760
RsaNI GTAC 2 cut(s) 478, 759
Rsr2I CGGWCCG 1 cut(s) 572
RsrII CGGWCCG 1 cut(s) 572
SaqAI TTAA 3 cut(s) 251, 320, 374
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 5 cut(s) 25, 279, 444, 675, 699
Sau96I GGNCC 3 cut(s) 19, 37, 572
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 640, 666
SduI GDGCHC 1 cut(s) 535
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 3 cut(s) 19, 37, 572
SphI GCATGC 2 cut(s) 402, 454
Sse9I AATT 3 cut(s) 289, 317, 321
SsiI CCGC 2 cut(s) 491, 762
SspMI CTAG 2 cut(s) 389, 753
StyD4I CCNGG 2 cut(s) 638, 664
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 3 cut(s) 115, 304, 461
TaiI ACGT 2 cut(s) 186, 483
TaqI TCGA 1 cut(s) 293
TaqII GACCGA 1 cut(s) 560
TasI AATT 3 cut(s) 289, 317, 321
TfiI GAWTC 2 cut(s) 159, 577
Tru1I TTAA 3 cut(s) 251, 320, 374
Tru9I TTAA 3 cut(s) 251, 320, 374
TscAI CASTG 1 cut(s) 651
TseFI GTSAC 2 cut(s) 516, 715
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 516, 715
TspDTI ATGAA 2 cut(s) 198, 371
TspRI CASTG 1 cut(s) 651
VpaK11BI GGWCC 3 cut(s) 19, 37, 572
VspI ATTAAT 1 cut(s) 320
XceI RCATGY 2 cut(s) 402, 454
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 2 cut(s) 389, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.