pycom10g25380

Secretory carrier-associated membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003098
Physical Location & Seq
Forward (+)
27043538 .. 27047583
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1221 bp
ATGATACGGGAGAAGATATCTTTATGTAGCATCACATTCACATCCCGCCTTTGGCTGTCAAAAGCTGACAAAAGAGCCGACGATTCATCCCTTTCTCTCTCTAGAAAAAAAAAAAAAAAATTCAGTAAACCAAAAGCAGCCGGTCTGCAGTCTACTCACTTTCTCTCTCTAGAAATTCCATTTCATCTCCTCACAGTTTCCCTTCATACTTTTGTGCCTGAAACTATATTTTTCTTCAATTTTCCTTCTTCCATCACAAGTCTTTTCTTTTATTCTCTTCTCATAAGCTTTCAGATCTCTCGGACAAGCTCTGAGACTGGTCCTTTGGGCGCTCAAATCTCTCTCCCCGCCATGGCTGGTCGCTACGACAGTAACCCATTTGCCGAAGAAGAAGAAGTCAATCCTTTCTCGAATCCTGGAATTGTTGCTCCTGCCAAAAACTCAAGACTTTCACCTCTTCCTCCTGAACGTGCTGGTTTCAACTACGGCCTTGGCGACCCCGTTGACATACCTATCGATGGAAATGCAGATTTGAAAAAGAGGGAGAGGGAACTCCAAGCTAAAGAAGCAGAATTGAGAAAGCGGGAGGAGATTGTAAGACGGAAAGAAGAAGCTGCCGCACGTGCTGGAATTGTTATTGAGGAAAAAAATTGGCCACCTTTCTTCCCAATTATCCATCATGATATTGCAAATGAAATACCCGTTCATTTACAGAGGGTGCAGTATGTGGCATTCACAACATGGTTAGGACTTGTTCTTTGCCTTTTCTGGAATGTAATAGCTGTTACTGCAGCATGGATTAAGGGGGAAGGGGTAAAAATTTGGTTCCTTGCAGTTATCTACTTCATATCAGGGGCTCCTGGATCCTATGTCTTGTGGTACCGTCCACTGTACCGTGTTTTCAGGTCCGAGAGTGCTTTGAAGTATGGATGGTTTTTCCTGTTTTATCTGGTCCACATTGGCTTCTGCATCTTTGCTGCGGTTGCTCCTCCGATATTCTTCAAAGGAAAATCTCTCACTGGCATTCTGCCTGCAATTGATGTTCTTGGTGACCATGTTTTGGTTGGGATCTTCTACTTCATTGGATTTGGAATGTTCTGTCTTGAATCAGTGCTGAGCATCTGGGTCATTCAGCAAGTATACATGTATTTCCGAGGAAGCGGTAAAGCTGCTGAAATGAGGCGTGAAGGAGCTAGAGGAGTCGTGAGGGCAGCACTTTGA

Protein Analysis

407

Amino Acids

45.82

Weight (kDa)

9.39

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 215 - 386 2.1e-59 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 879
AccB1I GGYRCC 1 cut(s) 879
AccB7I CCANNNNNTGG 1 cut(s) 1060
AccI GTMKAC 2 cut(s) 152, 1140
AciI CCGC 6 cut(s) 46, 348, 583, 618, 980, 1161
AclWI GGATC 3 cut(s) 858, 871, 1076
AcoI YGGCCR 1 cut(s) 653
AcsI RAATTY 3 cut(s) 119, 174, 819
AcvI CACGTG 1 cut(s) 623
AfaI GTAC 2 cut(s) 881, 893
AfiI CCNNNNNNNGG 4 cut(s) 51, 352, 518, 1060
AflIII ACRYGT 1 cut(s) 1143
AgsI TTSAA 6 cut(s) 238, 481, 535, 922, 1003, 1106
AjnI CCWGG 2 cut(s) 415, 859
AluBI AGCT 8 cut(s) 65, 288, 309, 560, 614, 782, 1169, 1193
AluI AGCT 8 cut(s) 65, 288, 309, 560, 614, 782, 1169, 1193
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 3 cut(s) 858, 871, 1076
AoxI GGCC 2 cut(s) 487, 653
ApeKI GCWGC 6 cut(s) 137, 614, 791, 977, 1169, 1211
ApoI RAATTY 3 cut(s) 119, 174, 819
ArsI GACNNNNNNTTYG 4 cut(s) 307, 339, 741, 773
Asp718I GGTACC 1 cut(s) 879
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 320, 906, 952
AsuHPI GGTGA 2 cut(s) 444, 1061
AvaII GGWCC 3 cut(s) 320, 906, 952
BalI TGGCCA 1 cut(s) 655
BamHI GGATCC 1 cut(s) 863
BanI GGYRCC 1 cut(s) 879
BanII GRGCYC 1 cut(s) 859
BbrPI CACGTG 1 cut(s) 623
BbvI GCAGC 5 cut(s) 149, 601, 803, 964, 1156
BccI CCATC 4 cut(s) 260, 512, 684, 924
BceAI ACGGC 1 cut(s) 502
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 2 cut(s) 417, 861
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 3 cut(s) 102, 170, 1194
BfmI CTRYAG 2 cut(s) 146, 789
BfoI RGCGCY 1 cut(s) 333
BglII AGATCT 1 cut(s) 294
BisI GCNGC 7 cut(s) 138, 615, 618, 792, 978, 1170, 1212
BlpI GCTNAGC 1 cut(s) 1115
BlsI GCNGC 7 cut(s) 139, 616, 619, 793, 979, 1171, 1213
Bme1390I CCNGG 2 cut(s) 417, 861
Bme18I GGWCC 3 cut(s) 320, 906, 952
BmgT120I GGNCC 3 cut(s) 320, 906, 952
BmiI GGNNCC 4 cut(s) 827, 858, 865, 881
BmrFI CCNGG 2 cut(s) 417, 861
BmsI GCATC 3 cut(s) 39, 978, 1128
Bpu1102I GCTNAGC 1 cut(s) 1115
BpuEI CTTGAG 1 cut(s) 427
Bsa29I ATCGAT 1 cut(s) 516
BsaAI YACGTR 1 cut(s) 623
BsaJI CCNNGG 3 cut(s) 351, 490, 1153
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 352, 518, 1060
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 322, 1024
BseBI CCWGG 2 cut(s) 417, 861
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 3 cut(s) 351, 490, 1153
BseGI GGATG 3 cut(s) 41, 86, 935
BseLI CCNNNNNNNGG 4 cut(s) 51, 352, 518, 1060
BseMII CTCAG 2 cut(s) 303, 1106
BseNI ACTGG 2 cut(s) 322, 1024
BseRI GAGGAG 4 cut(s) 179, 602, 978, 1212
BseXI GCAGC 5 cut(s) 149, 601, 803, 964, 1156
BsgI GTGCAG 1 cut(s) 740
BshFI GGCC 2 cut(s) 489, 655
BshNI GGYRCC 1 cut(s) 879
BshVI ATCGAT 1 cut(s) 516
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 4 cut(s) 51, 352, 518, 1060
BsmAI GTCTC 1 cut(s) 308
BsmI GAATGC 2 cut(s) 731, 1023
BsnI GGCC 2 cut(s) 489, 655
Bsp1286I GDGCHC 1 cut(s) 859
Bsp143I GATC 3 cut(s) 294, 863, 1068
Bsp1720I GCTNAGC 1 cut(s) 1115
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 6 cut(s) 46, 348, 583, 618, 980, 1161
BspANI GGCC 2 cut(s) 489, 655
BspCNI CTCAG 2 cut(s) 304, 1107
BspDI ATCGAT 1 cut(s) 516
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 827, 858, 865, 881
BspMAI CTGCAG 2 cut(s) 150, 793
BspPI GGATC 3 cut(s) 858, 871, 1076
BspT107I GGYRCC 1 cut(s) 879
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 322, 1024
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 351, 490, 1153
BssMI GATC 3 cut(s) 294, 863, 1068
BssNAI GTATAC 1 cut(s) 1141
BssT1I CCWWGG 2 cut(s) 351, 490
Bst1107I GTATAC 1 cut(s) 1141
Bst2UI CCWGG 2 cut(s) 417, 861
Bst4CI ACNGT 5 cut(s) 196, 371, 884, 891, 896
Bst6I CTCTTC 2 cut(s) 282, 462
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 1 cut(s) 1032
BstDEI CTNAG 2 cut(s) 312, 1115
BstDSI CCRYGG 1 cut(s) 351
BstEII GGTNACC 1 cut(s) 1049
BstF5I GGATG 3 cut(s) 41, 86, 935
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 297, 866, 1071
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 3 cut(s) 294, 863, 1068
BstMWI GCNNNNNNNGC 4 cut(s) 566, 623, 788, 983
BstNI CCWGG 2 cut(s) 417, 861
BstNSI RCATGY 1 cut(s) 1147
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 415, 859
BstSFI CTRYAG 2 cut(s) 146, 789
BstV1I GCAGC 5 cut(s) 149, 601, 803, 964, 1156
BstX2I RGATCY 3 cut(s) 294, 863, 1068
BstYI RGATCY 3 cut(s) 294, 863, 1068
BstZ17I GTATAC 1 cut(s) 1141
Bsu15I ATCGAT 1 cut(s) 516
BsuRI GGCC 2 cut(s) 489, 655
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 41, 86, 935
BtsIMutI CAGTG 3 cut(s) 887, 1017, 1116
Cac8I GCNNGC 1 cut(s) 1032
CciI TCATGA 1 cut(s) 679
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 320, 906, 952
ClaI ATCGAT 1 cut(s) 516
Csp6I GTAC 2 cut(s) 880, 892
CviAII CATG 6 cut(s) 352, 680, 741, 795, 1055, 1144
CviQI GTAC 2 cut(s) 880, 892
DdeI CTNAG 2 cut(s) 312, 1115
DpnI GATC 3 cut(s) 296, 865, 1070
DpnII GATC 3 cut(s) 294, 863, 1068
EaeI YGGCCR 1 cut(s) 653
Eam1104I CTCTTC 2 cut(s) 282, 462
EarI CTCTTC 2 cut(s) 282, 462
Eco130I CCWWGG 2 cut(s) 351, 490
Eco24I GRGCYC 1 cut(s) 859
Eco32I GATATC 1 cut(s) 18
Eco47I GGWCC 3 cut(s) 320, 906, 952
Eco72I CACGTG 1 cut(s) 623
Eco91I GGTNACC 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 1049
EcoRII CCWGG 2 cut(s) 415, 859
EcoRV GATATC 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 351, 490
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 2 cut(s) 351, 490
FaeI CATG 6 cut(s) 355, 683, 744, 798, 1058, 1147
FatI CATG 6 cut(s) 351, 679, 740, 794, 1054, 1143
FauI CCCGC 3 cut(s) 53, 355, 576
FblI GTMKAC 2 cut(s) 152, 1140
Fnu4HI GCNGC 7 cut(s) 138, 615, 618, 792, 978, 1170, 1212
FokI GGATG 3 cut(s) 28, 73, 942
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 7 cut(s) 138, 615, 618, 792, 978, 1170, 1212
FspBI CTAG 3 cut(s) 102, 170, 1194
GlaI GCGC 1 cut(s) 331
GluI GCNGC 7 cut(s) 138, 615, 618, 792, 978, 1170, 1212
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 489, 655
HapII CCGG 1 cut(s) 141
HhaI GCGC 1 cut(s) 332
Hin1II CATG 6 cut(s) 355, 683, 744, 798, 1058, 1147
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 4 cut(s) 83, 412, 1106, 1200
HpaII CCGG 1 cut(s) 141
HphI GGTGA 2 cut(s) 444, 1061
Hpy166II GTNNAC 6 cut(s) 128, 153, 505, 887, 955, 1141
Hpy188I TCNGA 6 cut(s) 294, 303, 313, 910, 993, 1154
Hpy188III TCNNGA 9 cut(s) 102, 170, 409, 444, 464, 680, 769, 1103, 1204
Hpy8I GTNNAC 6 cut(s) 128, 153, 505, 887, 955, 1141
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 212, 255, 803, 1181
HpyCH4III ACNGT 5 cut(s) 196, 371, 884, 891, 896
HpyCH4IV ACGT 2 cut(s) 469, 622
HpyCH4V TGCA 8 cut(s) 148, 527, 689, 721, 791, 833, 969, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 566, 623, 788, 983
HpyF3I CTNAG 2 cut(s) 312, 1115
HpySE526I ACGT 2 cut(s) 469, 622
Hsp92II CATG 6 cut(s) 355, 683, 744, 798, 1058, 1147
HspAI GCGC 1 cut(s) 330
KpnI GGTACC 1 cut(s) 883
Kzo9I GATC 3 cut(s) 294, 863, 1068
LmnI GCTCC 4 cut(s) 433, 862, 991, 1190
Lsp1109I GCAGC 5 cut(s) 149, 601, 803, 964, 1156
LweI GCATC 3 cut(s) 39, 978, 1128
MaeI CTAG 3 cut(s) 102, 170, 1194
MaeII ACGT 2 cut(s) 469, 622
MaeIII GTNAC 3 cut(s) 371, 784, 1049
MalI GATC 3 cut(s) 296, 865, 1070
MboI GATC 3 cut(s) 294, 863, 1068
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 3 cut(s) 294, 863, 1068
MhlI GDGCHC 1 cut(s) 859
MlsI TGGCCA 1 cut(s) 655
MluNI TGGCCA 1 cut(s) 655
MlyI GAGTC 1 cut(s) 1209
Mox20I TGGCCA 1 cut(s) 655
MscI TGGCCA 1 cut(s) 655
MseI TTAA 1 cut(s) 801
Msp20I TGGCCA 1 cut(s) 655
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 417, 861
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 2 cut(s) 731, 1023
MvaI CCWGG 2 cut(s) 417, 861
MwoI GCNNNNNNNGC 4 cut(s) 566, 623, 788, 983
NcoI CCATGG 1 cut(s) 351
NdeII GATC 3 cut(s) 294, 863, 1068
NlaIII CATG 6 cut(s) 355, 683, 744, 798, 1058, 1147
NlaIV GGNNCC 4 cut(s) 827, 858, 865, 881
NmuCI GTSAC 1 cut(s) 1049
NspI RCATGY 1 cut(s) 1147
PagI TCATGA 1 cut(s) 679
PciI ACATGT 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PctI GAATGC 2 cut(s) 731, 1023
PfeI GAWTC 3 cut(s) 83, 412, 1106
PflMI CCANNNNNTGG 1 cut(s) 1060
PfoI TCCNGGA 2 cut(s) 415, 859
PkrI GCNGC 7 cut(s) 139, 616, 619, 793, 979, 1171, 1213
PleI GAGTC 1 cut(s) 1208
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 1 cut(s) 1208
Ppu21I YACGTR 1 cut(s) 623
PscI ACATGT 1 cut(s) 1143
Psp6I CCWGG 2 cut(s) 415, 859
PspCI CACGTG 1 cut(s) 623
PspEI GGTNACC 1 cut(s) 1049
PspGI CCWGG 2 cut(s) 415, 859
PspN4I GGNNCC 4 cut(s) 827, 858, 865, 881
PspPI GGNCC 3 cut(s) 320, 906, 952
PstI CTGCAG 2 cut(s) 150, 793
PsuI RGATCY 3 cut(s) 294, 863, 1068
RsaI GTAC 2 cut(s) 881, 893
RsaNI GTAC 2 cut(s) 880, 892
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 7 cut(s) 138, 615, 618, 792, 978, 1170, 1212
Sau3AI GATC 3 cut(s) 294, 863, 1068
Sau96I GGNCC 3 cut(s) 320, 906, 952
SchI GAGTC 1 cut(s) 1209
ScrFI CCNGG 2 cut(s) 417, 861
SduI GDGCHC 1 cut(s) 859
SfaNI GCATC 3 cut(s) 39, 978, 1128
SfcI CTRYAG 2 cut(s) 146, 789
SinI GGWCC 3 cut(s) 320, 906, 952
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
SsiI CCGC 6 cut(s) 46, 348, 583, 618, 980, 1161
SspMI CTAG 3 cut(s) 102, 170, 1194
StyD4I CCNGG 2 cut(s) 415, 859
StyI CCWWGG 2 cut(s) 351, 490
TaaI ACNGT 5 cut(s) 196, 371, 884, 891, 896
TaiI ACGT 2 cut(s) 472, 625
TaqI TCGA 2 cut(s) 410, 516
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 3 cut(s) 83, 412, 1106
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 3 cut(s) 894, 1024, 1116
TseFI GTSAC 1 cut(s) 1049
TseI GCWGC 6 cut(s) 137, 614, 791, 977, 1169, 1211
Tsp45I GTSAC 1 cut(s) 1049
TspDTI ATGAA 7 cut(s) 75, 173, 194, 695, 708, 835, 1069
TspGWI ACGGA 1 cut(s) 616
TspRI CASTG 3 cut(s) 894, 1024, 1116
Van91I CCANNNNNTGG 1 cut(s) 1060
VpaK11BI GGWCC 3 cut(s) 320, 906, 952
XapI RAATTY 3 cut(s) 119, 174, 819
XbaI TCTAGA 2 cut(s) 101, 169
XceI RCATGY 1 cut(s) 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XmiI GTMKAC 2 cut(s) 152, 1140
XspI CTAG 3 cut(s) 102, 170, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.