pycom10g27640

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002609
Physical Location & Seq
Reverse (-)
28522825 .. 28525430
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGGCTTTGTTGGTGTCCAGAAGGCTTTTGCGTACTTTTGGGTCTGTATTAGCATCGGGCCGCTGTGACTCTCCAAGTAATTTATATCCATCATCATTTCGTGCTTCTCTCGATTTTTGCGGCCATGTTCCCGGGGTTATCTTACTTTCTGGAGAATTGTCAACTGTTTCCAAGGCTGGATCATTTTTTCTAGCAAATCGAAGGCTTTCAACCTCCAACCCGACTCCCGAATCAAGTGGAGGTGCCTTTCCCTCTAATTTATTGTCAGCAAGGCCCGTCGATGCATCAGACCGTTCTATAGGACTCGGTCAAGACCTTGTGATTCCAGTGACAAATTTTCATTATGAAGATAAAGGCTTCACAGTTTTGGCTGGTGATGTTTTCGATGTACCTATTAGGAAGGATATTATTCATCGGGTTGTACTATGGCAGCTAGCAAAACGGCAACAGGGAACACATTCAACAAAAACTATTAGTGAGGTTAGTGGGACTGGCAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCAAGGCACGGAACATTACGTGGCCCCCAGTTCCGAGGAGGATGCACAATGCATGGTCCTAAGCCACGAAGCCATGAATTTAAGCTGAATAAGAAGGTTCGGCGTCTAGGACTAAAGATTGCTCTGACAGCTCGGGCAGCAGAGGGAAAGCTTCTGGTTTTTGAAGATTTTGAGGTCCCTACGCATAAAACCAAGAATGTTGTGAACTACGTCCAACAAATGGAGGGCTCCAAGAAATTTCTGCTGGTGGATGGTACCAAAACTGAGGATGGTAAACAAATGTTACCTGAAAAGCTCAAGTTGGCTACACAAAATTTGCATTACGTGAACGTGCTGCCTGCAGTAGGTTTGAACGTCTACAGCATCCTGCAGCATGACACACTGGTGATGACCCAAGCTGCTGTAAATGAAATTGTCGAGCGGATGCACACCCCAATCAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

325

Amino Acids

35.65

Weight (kDa)

10.07

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 123 - 317 3.4e-69 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 2 cut(s) 242, 782
AccB7I CCANNNNNTGG 1 cut(s) 748
AccBSI CCGCTC 1 cut(s) 949
AccI GTMKAC 1 cut(s) 885
AciI CCGC 3 cut(s) 61, 120, 949
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 4 cut(s) 334, 605, 764, 841
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 5 cut(s) 34, 390, 423, 520, 784
AfiI CCNNNNNNNGG 2 cut(s) 748, 872
AgsI TTSAA 4 cut(s) 210, 462, 692, 880
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 6 cut(s) 433, 613, 659, 679, 823, 926
AluI AGCT 6 cut(s) 433, 613, 659, 679, 823, 926
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 2 cut(s) 131, 660
AoxI GGCC 4 cut(s) 58, 121, 272, 550
ApeKI GCWGC 5 cut(s) 430, 665, 862, 898, 926
ApoI RAATTY 4 cut(s) 334, 605, 764, 841
Asp700I GAANNNNTTC 2 cut(s) 205, 457
Asp718I GGTACC 1 cut(s) 782
AspS9I GGNCC 5 cut(s) 58, 273, 551, 584, 703
AsuC2I CCSGG 2 cut(s) 132, 133
AsuHPI GGTGA 2 cut(s) 386, 925
AsuNHI GCTAGC 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 131, 660
AvaII GGWCC 2 cut(s) 584, 703
BanI GGYRCC 2 cut(s) 242, 782
BanII GRGCYC 1 cut(s) 758
BbvI GCAGC 5 cut(s) 442, 677, 849, 910, 913
BccI CCATC 3 cut(s) 97, 773, 791
BceAI ACGGC 1 cut(s) 458
BcgI CGANNNNNNTGC 2 cut(s) 552, 586
BcnI CCSGG 2 cut(s) 132, 133
BfaI CTAG 3 cut(s) 191, 434, 635
BfmI CTRYAG 4 cut(s) 297, 867, 886, 896
BisI GCNGC 7 cut(s) 61, 121, 431, 666, 863, 899, 927
BlsI GCNGC 7 cut(s) 62, 122, 432, 667, 864, 900, 928
Bme1390I CCNGG 2 cut(s) 132, 133
Bme18I GGWCC 2 cut(s) 584, 703
BmeT110I CYCGRG 2 cut(s) 131, 660
BmgT120I GGNCC 5 cut(s) 58, 273, 551, 584, 703
BmiI GGNNCC 5 cut(s) 244, 553, 705, 757, 784
BmrFI CCNGG 2 cut(s) 132, 133
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 6 cut(s) 62, 271, 293, 560, 900, 942
BmtI GCTAGC 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 809
BpuMI CCSGG 2 cut(s) 132, 133
BsaAI YACGTR 2 cut(s) 548, 853
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 5 cut(s) 131, 132, 171, 503, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 872
Bse1I ACTGG 5 cut(s) 326, 496, 526, 556, 915
BseDI CCNNGG 5 cut(s) 131, 132, 171, 503, 562
BseGI GGATG 5 cut(s) 575, 784, 802, 891, 957
BseLI CCNNNNNNNGG 2 cut(s) 748, 872
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 5 cut(s) 326, 496, 526, 556, 915
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 5 cut(s) 442, 677, 849, 910, 913
BshFI GGCC 4 cut(s) 60, 123, 274, 552
BshNI GGYRCC 2 cut(s) 242, 782
BsiHKCI CYCGRG 2 cut(s) 131, 660
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 2 cut(s) 502, 689
BslI CCNNNNNNNGG 2 cut(s) 748, 872
BsmFI GGGAC 2 cut(s) 502, 689
BsnI GGCC 4 cut(s) 60, 123, 274, 552
BsoBI CYCGRG 2 cut(s) 131, 660
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 3 cut(s) 61, 120, 949
BspANI GGCC 4 cut(s) 60, 123, 274, 552
BspCNI CTCAG 1 cut(s) 784
BspLI GGNNCC 5 cut(s) 244, 553, 705, 757, 784
BspMAI CTGCAG 2 cut(s) 871, 900
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 187
BspT107I GGYRCC 2 cut(s) 242, 782
BsrBI CCGCTC 1 cut(s) 949
BsrI ACTGG 5 cut(s) 326, 496, 526, 556, 915
BssECI CCNNGG 5 cut(s) 131, 132, 171, 503, 562
BssMI GATC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 631
BssT1I CCWWGG 2 cut(s) 171, 503
Bst4CI ACNGT 4 cut(s) 166, 293, 364, 971
BstACI GRCGYC 1 cut(s) 631
BstBAI YACGTR 2 cut(s) 548, 853
BstC8I GCNNGC 2 cut(s) 435, 867
BstDEI CTNAG 2 cut(s) 588, 792
BstDSI CCRYGG 1 cut(s) 503
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 5 cut(s) 575, 784, 802, 891, 957
BstKTI GATC 1 cut(s) 182
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 656, 665
BstSCI CCNGG 2 cut(s) 130, 131
BstSFI CTRYAG 4 cut(s) 297, 867, 886, 896
BstV1I GCAGC 5 cut(s) 442, 677, 849, 910, 913
BsuRI GGCC 4 cut(s) 60, 123, 274, 552
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 5 cut(s) 575, 784, 802, 891, 957
BtsIMutI CAGTG 2 cut(s) 333, 908
Cac8I GCNNGC 2 cut(s) 435, 867
Cfr13I GGNCC 5 cut(s) 58, 273, 551, 584, 703
Cfr9I CCCGGG 1 cut(s) 131
CseI GACGC 1 cut(s) 620
Csp6I GTAC 5 cut(s) 33, 389, 422, 519, 783
CviAII CATG 5 cut(s) 125, 504, 581, 602, 902
CviQI GTAC 5 cut(s) 33, 389, 422, 519, 783
DdeI CTNAG 2 cut(s) 588, 792
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 171, 503
Eco24I GRGCYC 1 cut(s) 758
Eco47I GGWCC 2 cut(s) 584, 703
Eco88I CYCGRG 2 cut(s) 131, 660
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO109I RGGNCCY 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 171, 503
EcoT22I ATGCAT 2 cut(s) 286, 582
EcoT38I GRGCYC 1 cut(s) 758
ErhI CCWWGG 2 cut(s) 171, 503
FaeI CATG 5 cut(s) 128, 507, 584, 605, 905
FaqI GGGAC 2 cut(s) 502, 689
FatI CATG 5 cut(s) 124, 503, 580, 601, 901
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 7 cut(s) 61, 121, 431, 666, 863, 899, 927
FokI GGATG 5 cut(s) 582, 791, 809, 878, 964
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 7 cut(s) 61, 121, 431, 666, 863, 899, 927
FspBI CTAG 3 cut(s) 191, 434, 635
GluI GCNGC 7 cut(s) 61, 121, 431, 666, 863, 899, 927
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 4 cut(s) 60, 123, 274, 552
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 5 cut(s) 128, 507, 584, 605, 905
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 6 cut(s) 68, 223, 230, 303, 322, 508
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 386, 925
Hpy166II GTNNAC 5 cut(s) 162, 733, 803, 856, 886
Hpy188I TCNGA 4 cut(s) 289, 513, 563, 654
Hpy188III TCNNGA 5 cut(s) 18, 110, 150, 227, 311
Hpy8I GTNNAC 5 cut(s) 162, 733, 803, 856, 886
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 5 cut(s) 15, 195, 394, 508, 616
HpyCH4III ACNGT 4 cut(s) 166, 293, 364, 971
HpyCH4IV ACGT 5 cut(s) 547, 738, 852, 858, 882
HpyCH4V TGCA 7 cut(s) 284, 573, 580, 847, 869, 898, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 665
HpyF3I CTNAG 2 cut(s) 588, 792
HpySE526I ACGT 5 cut(s) 547, 738, 852, 858, 882
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 5 cut(s) 128, 507, 584, 605, 905
KpnI GGTACC 1 cut(s) 786
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 761
Lsp1109I GCAGC 5 cut(s) 442, 677, 849, 910, 913
LweI GCATC 6 cut(s) 62, 271, 293, 560, 900, 942
MaeI CTAG 3 cut(s) 191, 434, 635
MaeII ACGT 5 cut(s) 547, 738, 852, 858, 882
MaeIII GTNAC 3 cut(s) 65, 328, 810
MalI GATC 1 cut(s) 181
MbiI CCGCTC 1 cut(s) 949
MboI GATC 1 cut(s) 179
MboII GAAGA 2 cut(s) 359, 704
MhlI GDGCHC 1 cut(s) 758
MluCI AATT 8 cut(s) 79, 155, 256, 334, 605, 764, 841, 939
MlyI GAGTC 3 cut(s) 62, 217, 297
MmeI TCCRAC 2 cut(s) 240, 766
Mph1103I ATGCAT 2 cut(s) 286, 582
MroXI GAANNNNTTC 2 cut(s) 205, 457
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 911
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 132, 133
MwoI GCNNNNNNNGC 2 cut(s) 656, 665
NciI CCSGG 2 cut(s) 132, 133
NcoI CCATGG 1 cut(s) 503
NdeII GATC 1 cut(s) 179
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 5 cut(s) 128, 507, 584, 605, 905
NlaIV GGNNCC 5 cut(s) 244, 553, 705, 757, 784
NmuCI GTSAC 2 cut(s) 65, 328
NsiI ATGCAT 2 cut(s) 286, 582
OliI CACNNNNGTG 1 cut(s) 911
PdmI GAANNNNTTC 2 cut(s) 205, 457
PfeI GAWTC 3 cut(s) 230, 322, 508
PflFI GACNNNGTC 1 cut(s) 306
PflMI CCANNNNNTGG 1 cut(s) 748
PkrI GCNGC 7 cut(s) 62, 122, 432, 667, 864, 900, 928
PleI GAGTC 3 cut(s) 62, 217, 297
PpsI GAGTC 3 cut(s) 62, 217, 297
Ppu21I YACGTR 2 cut(s) 548, 853
PpuMI RGGWCCY 1 cut(s) 703
Psp5II RGGWCCY 1 cut(s) 703
PspN4I GGNNCC 5 cut(s) 244, 553, 705, 757, 784
PspPI GGNCC 5 cut(s) 58, 273, 551, 584, 703
PspPPI RGGWCCY 1 cut(s) 703
PstI CTGCAG 2 cut(s) 871, 900
PsyI GACNNNGTC 1 cut(s) 306
RsaI GTAC 5 cut(s) 34, 390, 423, 520, 784
RsaNI GTAC 5 cut(s) 33, 389, 422, 519, 783
RseI CAYNNNNRTG 1 cut(s) 911
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 7 cut(s) 61, 121, 431, 666, 863, 899, 927
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 5 cut(s) 58, 273, 551, 584, 703
SchI GAGTC 3 cut(s) 62, 217, 297
ScrFI CCNGG 2 cut(s) 132, 133
SduI GDGCHC 1 cut(s) 758
SfaNI GCATC 6 cut(s) 62, 271, 293, 560, 900, 942
SfcI CTRYAG 4 cut(s) 297, 867, 886, 896
SinI GGWCC 2 cut(s) 584, 703
SmaI CCCGGG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 8 cut(s) 79, 155, 256, 334, 605, 764, 841, 939
SsiI CCGC 3 cut(s) 61, 120, 949
SspMI CTAG 3 cut(s) 191, 434, 635
StyD4I CCNGG 2 cut(s) 130, 131
StyI CCWWGG 2 cut(s) 171, 503
TaaI ACNGT 4 cut(s) 166, 293, 364, 971
TaiI ACGT 5 cut(s) 550, 741, 855, 861, 885
TaqI TCGA 6 cut(s) 111, 199, 279, 384, 526, 945
TaqII GACCGA 1 cut(s) 296
TasI AATT 8 cut(s) 79, 155, 256, 334, 605, 764, 841, 939
TatI WGTACW 1 cut(s) 421
TauI GCSGC 2 cut(s) 63, 123
TfiI GAWTC 3 cut(s) 230, 322, 508
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 2 cut(s) 333, 915
TseFI GTSAC 2 cut(s) 65, 328
TseI GCWGC 5 cut(s) 430, 665, 862, 898, 926
Tsp45I GTSAC 2 cut(s) 65, 328
TspDTI ATGAA 5 cut(s) 329, 360, 401, 618, 951
TspGWI ACGGA 1 cut(s) 552
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 2 cut(s) 333, 915
Tth111I GACNNNGTC 1 cut(s) 306
Van91I CCANNNNNTGG 1 cut(s) 748
VpaK11BI GGWCC 2 cut(s) 584, 703
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 4 cut(s) 334, 605, 764, 841
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 205, 457
XspI CTAG 3 cut(s) 191, 434, 635
Zsp2I ATGCAT 2 cut(s) 286, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.