pycom111g01580

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005675
Physical Location & Seq
Forward (+)
981590 .. 983045
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGCGAATTTGCTGATTAACAGGACCCTAAGATCAGTCGGAAACCTCAAACTGATCGGATCTCTCTCTTCTCGAGCTCGCCACTTCAGCTCCACCGCCACCGCCACCGCCGAGCAGCCCCAATCCGATGCTCCATCTTCGTCATTCACCTTCTCCTCCGAGGGAAAAGAAGACAAGAACAACAGCAGTGCCCACGATGACAGCATATACGTCAAGTCGCCGTCGAGTTCGAGCTCCAAGAGCGAAGCGGCGTCGTCGGTGACGATGCCGATGTCGTTCATGACGGGGTCCATCGTGGGAAAGAGATTTTACAAACAGGTGACGACGAGGCCGGCCGACGACGGCAATGGCTGGACTGTGATGCTCGATTACCGGACACTCAAAACGCCATCGAAGAGGCCCCTAAAGCTTCCTACTTTAGCTCTGGCGAAGGCCATTGCGGCGGAATGGGAATACCAGGAAACAGATGGGATTAGGCCTTTTACAATGCCTCTCATGAAGCATGCTTGTACTGCACTGGAAAGAGTTCCTCTCACACGGCCAAAGGTTATAGATTATTTGATGAAGAAATTTAATCAAGATTTAGTCTTCTGTCGCGCCCCAGATGACAATGATATCACAAGTGTTGTCCATGACCGACAAGTGGAAAAAATGGATCCTTTGCTTGAGTGGGTGAAGTCAGAATTTGGCTTCAAGCCTGCTGTGTACTCGAGCTTTTTTGGTGGGAAGCAGGAGGATGGCCTCACAAGGGCTATAGAGACCTTACTAAAACAAACAGATGACTGTGAATTGGCAGCAATTGATGCCATCGCATCGTCAGCACACTCTCTAATAATTGCCATCGGAATTTTTCGTGGGAAGTGA

Protein Analysis

288

Amino Acids

31.6

Weight (kDa)

8.64

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP12 PF07542 101 - 225 7.8e-31 ATP12 chaperone protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 597
AciI CCGC 6 cut(s) 96, 102, 108, 248, 440, 443
AclWI GGATC 3 cut(s) 67, 650, 663
AcoI YGGCCR 2 cut(s) 333, 539
AcsI RAATTY 4 cut(s) 7, 569, 683, 846
AcuI CTGAAG 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 2 cut(s) 511, 707
AfiI CCNNNNNNNGG 2 cut(s) 643, 748
AgsI TTSAA 1 cut(s) 694
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 6 cut(s) 77, 90, 234, 409, 422, 714
AluI AGCT 6 cut(s) 77, 90, 234, 409, 422, 714
Alw21I GWGCWC 2 cut(s) 79, 236
Alw26I GTCTC 1 cut(s) 752
AlwI GGATC 3 cut(s) 67, 650, 663
Ama87I CYCGRG 2 cut(s) 72, 709
AoxI GGCC 7 cut(s) 329, 333, 398, 432, 476, 539, 739
ApeKI GCWGC 2 cut(s) 115, 794
ApoI RAATTY 4 cut(s) 7, 569, 683, 846
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 3 cut(s) 24, 288, 399
AsuHPI GGTGA 4 cut(s) 139, 271, 331, 685
AvaI CYCGRG 2 cut(s) 72, 709
AvaII GGWCC 2 cut(s) 24, 288
BaeGI GKGCMC 1 cut(s) 193
BamHI GGATCC 1 cut(s) 655
BanII GRGCYC 2 cut(s) 79, 236
BbsI GAAGAC 2 cut(s) 177, 580
Bbv12I GWGCWC 2 cut(s) 79, 236
BbvI GCAGC 2 cut(s) 127, 806
BccI CCATC 7 cut(s) 142, 299, 397, 461, 731, 815, 848
BceAI ACGGC 3 cut(s) 205, 358, 554
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 752
BfmI CTRYAG 1 cut(s) 753
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 4 cut(s) 116, 249, 441, 795
BlsI GCNGC 4 cut(s) 117, 250, 442, 796
Bme1390I CCNGG 1 cut(s) 458
Bme18I GGWCC 2 cut(s) 24, 288
BmeT110I CYCGRG 2 cut(s) 72, 709
BmgT120I GGNCC 3 cut(s) 24, 288, 399
BmiI GGNNCC 4 cut(s) 26, 289, 401, 657
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 5 cut(s) 118, 255, 351, 793, 821
BpiI GAAGAC 2 cut(s) 177, 580
BplI GAGNNNNNCTC 2 cut(s) 516, 548
BpuEI CTTGAG 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 748
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 522
Bse3DI GCAATG 2 cut(s) 352, 435
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 742
BseLI CCNNNNNNNGG 2 cut(s) 643, 748
BseMI GCAATG 2 cut(s) 352, 435
BseNI ACTGG 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 193
BseX3I CGGCCG 1 cut(s) 333
BseXI GCAGC 2 cut(s) 127, 806
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 1 cut(s) 597
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 7 cut(s) 331, 335, 400, 434, 478, 541, 741
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 2 cut(s) 79, 236
BsiHKCI CYCGRG 2 cut(s) 72, 709
BsiSI CCGG 2 cut(s) 332, 373
BslI CCNNNNNNNGG 2 cut(s) 643, 748
BsmAI GTCTC 1 cut(s) 752
BsnI GGCC 7 cut(s) 331, 335, 400, 434, 478, 541, 741
Bso31I GGTCTC 1 cut(s) 752
BsoBI CYCGRG 2 cut(s) 72, 709
Bsp1286I GDGCHC 3 cut(s) 79, 193, 236
Bsp143I GATC 4 cut(s) 32, 54, 59, 655
BspACI CCGC 6 cut(s) 96, 102, 108, 248, 440, 443
BspANI GGCC 7 cut(s) 331, 335, 400, 434, 478, 541, 741
BspFNI CGCG 1 cut(s) 597
BspHI TCATGA 2 cut(s) 279, 495
BspLI GGNNCC 4 cut(s) 26, 289, 401, 657
BspPI GGATC 3 cut(s) 67, 650, 663
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 2 cut(s) 352, 435
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 522
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 4 cut(s) 32, 54, 59, 655
BssNI GRCGYC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 358, 785
Bst6I CTCTTC 2 cut(s) 73, 389
BstACI GRCGYC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 803
BstC8I GCNNGC 4 cut(s) 79, 333, 504, 699
BstDEI CTNAG 1 cut(s) 29
BstF5I GGATG 1 cut(s) 742
BstFNI CGCG 1 cut(s) 597
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 4 cut(s) 35, 57, 62, 658
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 4 cut(s) 32, 54, 59, 655
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 7 cut(s) 87, 240, 406, 440, 512, 803, 818
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 1 cut(s) 753
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 127, 806
BstV2I GAAGAC 2 cut(s) 177, 580
BstX2I RGATCY 2 cut(s) 59, 655
BstYI RGATCY 2 cut(s) 59, 655
BstZI CGGCCG 1 cut(s) 333
BsuRI GGCC 7 cut(s) 331, 335, 400, 434, 478, 541, 741
BtgZI GCGATG 1 cut(s) 793
BtsCI GGATG 1 cut(s) 742
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 193, 515
Cac8I GCNNGC 4 cut(s) 79, 333, 504, 699
CciI TCATGA 2 cut(s) 279, 495
CfoI GCGC 1 cut(s) 599
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 24, 288, 399
CseI GACGC 1 cut(s) 240
Csp6I GTAC 2 cut(s) 510, 706
CviAII CATG 4 cut(s) 280, 496, 503, 632
CviQI GTAC 2 cut(s) 510, 706
DdeI CTNAG 1 cut(s) 29
DpnI GATC 4 cut(s) 34, 56, 61, 657
DpnII GATC 4 cut(s) 32, 54, 59, 655
EaeI YGGCCR 2 cut(s) 333, 539
EagI CGGCCG 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 73, 389
EarI CTCTTC 2 cut(s) 73, 389
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 2 cut(s) 77, 234
EclXI CGGCCG 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 79, 236
Eco31I GGTCTC 1 cut(s) 752
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 24, 288
Eco52I CGGCCG 1 cut(s) 333
Eco53kI GAGCTC 2 cut(s) 77, 234
Eco57I CTGAAG 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 72, 709
EcoICRI GAGCTC 2 cut(s) 77, 234
EcoO109I RGGNCCY 2 cut(s) 24, 399
EcoRII CCWGG 1 cut(s) 456
EcoRV GATATC 1 cut(s) 616
EcoT38I GRGCYC 2 cut(s) 79, 236
FaeI CATG 4 cut(s) 283, 499, 506, 635
FaiI YATR 8 cut(s) 206, 208, 281, 497, 504, 551, 633, 755
FatI CATG 4 cut(s) 279, 495, 502, 631
Fnu4HI GCNGC 4 cut(s) 116, 249, 441, 795
FokI GGATG 1 cut(s) 749
FriOI GRGCYC 2 cut(s) 79, 236
FseI GGCCGGCC 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 116, 249, 441, 795
GlaI GCGC 1 cut(s) 598
GluI GCNGC 4 cut(s) 116, 249, 441, 795
HaeIII GGCC 7 cut(s) 331, 335, 400, 434, 478, 541, 741
HapII CCGG 2 cut(s) 332, 373
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 599
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 4 cut(s) 283, 499, 506, 635
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 407
HpaII CCGG 2 cut(s) 332, 373
HphI GGTGA 4 cut(s) 139, 271, 331, 685
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 6 cut(s) 41, 59, 127, 160, 682, 845
Hpy188III TCNNGA 4 cut(s) 72, 280, 496, 578
Hpy8I GTNNAC 1 cut(s) 706
Hpy99I CGWCG 6 cut(s) 226, 256, 259, 328, 341, 344
HpyAV CCTTC 2 cut(s) 160, 424
HpyCH4III ACNGT 2 cut(s) 358, 785
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 1 cut(s) 515
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 240, 406, 440, 512, 803, 818
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 4 cut(s) 283, 499, 506, 635
HspAI GCGC 1 cut(s) 597
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Kzo9I GATC 4 cut(s) 32, 54, 59, 655
LmnI GCTCC 3 cut(s) 95, 136, 239
Lsp1109I GCAGC 2 cut(s) 127, 806
LweI GCATC 5 cut(s) 118, 255, 351, 793, 821
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 259, 319
MalI GATC 4 cut(s) 34, 56, 61, 657
MboI GATC 4 cut(s) 32, 54, 59, 655
MboII GAAGA 6 cut(s) 60, 129, 182, 406, 577, 580
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 2 cut(s) 59, 655
MhlI GDGCHC 3 cut(s) 79, 193, 236
MluCI AATT 7 cut(s) 7, 569, 683, 788, 798, 834, 846
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 9 cut(s) 56, 154, 166, 321, 390, 501, 540, 727, 752
MroNI GCCGGC 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 2 cut(s) 18, 573
MspI CCGG 2 cut(s) 332, 373
MspR9I CCNGG 1 cut(s) 458
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 7 cut(s) 87, 240, 406, 440, 512, 803, 818
NaeI GCCGGC 1 cut(s) 333
NdeII GATC 4 cut(s) 32, 54, 59, 655
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 4 cut(s) 283, 499, 506, 635
NlaIV GGNNCC 4 cut(s) 26, 289, 401, 657
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 259, 319
NspI RCATGY 1 cut(s) 506
PaeI GCATGC 1 cut(s) 506
PaeR7I CTCGAG 2 cut(s) 72, 709
PagI TCATGA 2 cut(s) 279, 495
PceI AGGCCT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 525
PflFI GACNNNGTC 1 cut(s) 286
PkrI GCNGC 4 cut(s) 117, 250, 442, 796
PpuMI RGGWCCY 1 cut(s) 24
Psp124BI GAGCTC 2 cut(s) 79, 236
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 4 cut(s) 26, 289, 401, 657
PspPI GGNCC 3 cut(s) 24, 288, 399
PspPPI RGGWCCY 1 cut(s) 24
PspXI VCTCGAGB 1 cut(s) 709
PsuI RGATCY 2 cut(s) 59, 655
PsyI GACNNNGTC 1 cut(s) 286
RigI GGCCGGCC 1 cut(s) 335
RsaI GTAC 2 cut(s) 511, 707
RsaNI GTAC 2 cut(s) 510, 706
SacI GAGCTC 2 cut(s) 79, 236
SaqAI TTAA 2 cut(s) 18, 573
SatI GCNGC 4 cut(s) 116, 249, 441, 795
Sau3AI GATC 4 cut(s) 32, 54, 59, 655
Sau96I GGNCC 3 cut(s) 24, 288, 399
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 3 cut(s) 79, 193, 236
SfaNI GCATC 5 cut(s) 118, 255, 351, 793, 821
SfcI CTRYAG 1 cut(s) 753
Sfr274I CTCGAG 2 cut(s) 72, 709
SinI GGWCC 2 cut(s) 24, 288
SlaI CTCGAG 2 cut(s) 72, 709
SmlI CTYRAG 3 cut(s) 72, 665, 709
SmoI CTYRAG 3 cut(s) 72, 665, 709
SphI GCATGC 1 cut(s) 506
Sse9I AATT 7 cut(s) 7, 569, 683, 788, 798, 834, 846
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 6 cut(s) 96, 102, 108, 248, 440, 443
SstI GAGCTC 2 cut(s) 79, 236
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 358, 785
TaiI ACGT 1 cut(s) 213
TaqI TCGA 6 cut(s) 73, 224, 230, 366, 392, 710
TaqII GACCGA 1 cut(s) 651
TasI AATT 7 cut(s) 7, 569, 683, 788, 798, 834, 846
TatI WGTACW 2 cut(s) 509, 705
TauI GCSGC 2 cut(s) 251, 443
Tru1I TTAA 2 cut(s) 18, 573
Tru9I TTAA 2 cut(s) 18, 573
TscAI CASTG 2 cut(s) 193, 522
TseFI GTSAC 2 cut(s) 259, 319
TseI GCWGC 2 cut(s) 115, 794
Tsp45I GTSAC 2 cut(s) 259, 319
TspDTI ATGAA 3 cut(s) 268, 512, 578
TspRI CASTG 2 cut(s) 193, 522
Tth111I GACNNNGTC 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 24, 288
XapI RAATTY 4 cut(s) 7, 569, 683, 846
XceI RCATGY 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XhoI CTCGAG 2 cut(s) 72, 709
XmnI GAANNNNTTC 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.