pycom11g09580

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002733
Physical Location & Seq
Reverse (-)
7821902 .. 7824881
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGGGTCCTGTGAGAGGGATTAGGAAGAGAAAGAAGGCTGAGAAGAAGGGTGAGGAGAATGCTTCTGGTTCTGGGTCATCAGAGAAGGAAGGTCCTGTGGATTGGTGGGATGAATTCTCCAGGAGAATTAATGGTGTGCAGTCTCCATCAAAGGTTTTAGACAAGTTTGAATCTGTTTTTAAGCTATCCAGGAAAACCTTCGACTACATCTGTTCACTTGTGAGTGAAGATATGATGGCAAAGTCGGCACATTTTGTGTTTACAAACGGTAAGCCCTTGTCTTTGCACGATCAAGTCGCTGTAGCCTTAAGAAGATTGAGCTCGGGTGAGTCACTAGTGACAATTGGCGATTCGTTTGGGGTGAACCACTCAACTGTGTCCCAAGTGACCTGGCGATTTGTCGAAGCCATGGAAGAAAGAGGACTTCACCACCTGCAATGGCCTTCCACTGAAGCAGAAATGACAGAAGTTAAATCCAAATTTCAGAAAATACGAGGCTTTCCCAATTGTTGCGGAGTTGTGGATACCACTCACATCATGATGTGTTTGCCTGCTTCAGACCCCACAAGTAACGCATGGCTCGATCAAGAGAAGAATCACAGCATGGTCTTGCAAGCCATTGTGGACCCTGACATGAGGTTTCGGGACATAGTTACCGGATGGCCAGGAAAAATGAAGGACTGGTCGGTGTTTCAGAGTTCGAATTTTTATCAACTCTGTGCTAGAGGTGAGAGATTGAATGGGAAAAGGTTAGAGCTCTCCACAGGATTGGATATCCGGGAATACATACTTGGGGACTTGGGGTTTTCTTTATTGCCCTATCTCGTGATCGATCGTAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

282

Amino Acids

31.73

Weight (kDa)

7.67

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 174 - 275 1.8e-11 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 441
AciI CCGC 1 cut(s) 513
AcoI YGGCCR 1 cut(s) 662
AcsI RAATTY 3 cut(s) 113, 479, 703
AcuI CTGAAG 2 cut(s) 471, 540
AfiI CCNNNNNNNGG 1 cut(s) 14
AflII CTTAAG 1 cut(s) 307
AgsI TTSAA 2 cut(s) 170, 739
AhlI ACTAGT 1 cut(s) 334
AjnI CCWGG 4 cut(s) 119, 188, 389, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 3 cut(s) 184, 321, 757
AluI AGCT 3 cut(s) 184, 321, 757
Alw21I GWGCWC 2 cut(s) 323, 759
Alw26I GTCTC 1 cut(s) 147
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 2 cut(s) 440, 662
ApoI RAATTY 3 cut(s) 113, 479, 703
AseI ATTAAT 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 197
AspS9I GGNCC 3 cut(s) 5, 92, 625
AsuC2I CCSGG 1 cut(s) 779
AsuHPI GGTGA 5 cut(s) 62, 338, 373, 419, 740
AsuII TTCGAA 1 cut(s) 701
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 3 cut(s) 5, 92, 625
BalI TGGCCA 1 cut(s) 664
BanII GRGCYC 2 cut(s) 323, 759
BauI CACGAG 1 cut(s) 824
Bbv12I GWGCWC 2 cut(s) 323, 759
BccI CCATC 3 cut(s) 154, 229, 654
BciT130I CCWGG 4 cut(s) 121, 190, 391, 666
BciVI GTATCC 1 cut(s) 517
BcnI CCSGG 1 cut(s) 779
BcoDI GTCTC 1 cut(s) 147
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 2 cut(s) 335, 723
BfmI CTRYAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 307
BfuAI ACCTGC 1 cut(s) 441
BfuI GTATCC 1 cut(s) 517
Bme1390I CCNGG 5 cut(s) 121, 190, 391, 666, 779
Bme18I GGWCC 3 cut(s) 5, 92, 625
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 5, 92, 625
BmiI GGNNCC 2 cut(s) 6, 627
BmrFI CCNGG 5 cut(s) 121, 190, 391, 666, 779
BpmI CTGGAG 1 cut(s) 103
Bpu14I TTCGAA 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 779
Bsa29I ATCGAT 1 cut(s) 831
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 443
BseBI CCWGG 4 cut(s) 121, 190, 391, 666
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 2 cut(s) 115, 665
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMI GCAATG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 686
BseRI GAGGAG 1 cut(s) 68
BsgI GTGCAG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 835
BshFI GGCC 2 cut(s) 442, 664
BshVI ATCGAT 1 cut(s) 831
BsiEI CGRYCG 1 cut(s) 835
BsiHKAI GWGCWC 2 cut(s) 323, 759
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 2 cut(s) 657, 778
BslFI GGGAC 3 cut(s) 364, 659, 809
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 3 cut(s) 364, 659, 809
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 2 cut(s) 442, 664
BsoBI CYCGRG 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 323, 759
Bsp143I GATC 4 cut(s) 289, 583, 828, 832
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 1 cut(s) 513
BspANI GGCC 2 cut(s) 442, 664
BspCNI CTCAG 1 cut(s) 31
BspDI ATCGAT 1 cut(s) 831
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 6, 627
BspMI ACCTGC 1 cut(s) 441
BspT104I TTCGAA 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 443
BsrI ACTGG 1 cut(s) 686
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 4 cut(s) 289, 583, 828, 832
BssSI CACGAG 1 cut(s) 824
BssT1I CCWWGG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 824
Bst2UI CCWGG 4 cut(s) 121, 190, 391, 666
Bst4CI ACNGT 2 cut(s) 269, 376
Bst6I CTCTTC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 307
BstBI TTCGAA 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 552, 615
BstDEI CTNAG 1 cut(s) 39
BstDSI CCRYGG 1 cut(s) 408
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 115, 665
BstKTI GATC 4 cut(s) 292, 586, 831, 835
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 4 cut(s) 289, 583, 828, 832
BstMCI CGRYCG 1 cut(s) 835
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 4 cut(s) 121, 190, 391, 666
BstSCI CCNGG 5 cut(s) 119, 188, 389, 664, 777
BstSFI CTRYAG 1 cut(s) 300
BstXI CCANNNNNNTGG 1 cut(s) 769
Bsu15I ATCGAT 1 cut(s) 831
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 2 cut(s) 442, 664
BsuTUI ATCGAT 1 cut(s) 831
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 115, 665
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 552, 615
CciI TCATGA 1 cut(s) 537
Cfr13I GGNCC 3 cut(s) 5, 92, 625
ClaI ATCGAT 1 cut(s) 831
CviAII CATG 5 cut(s) 409, 538, 576, 604, 634
DdeI CTNAG 1 cut(s) 39
DpnI GATC 4 cut(s) 291, 585, 830, 834
DpnII GATC 4 cut(s) 289, 583, 828, 832
EaeI YGGCCR 1 cut(s) 662
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 321, 757
Eco130I CCWWGG 1 cut(s) 408
Eco24I GRGCYC 2 cut(s) 323, 759
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 3 cut(s) 5, 92, 625
Eco53kI GAGCTC 2 cut(s) 321, 757
Eco57I CTGAAG 2 cut(s) 471, 540
Eco88I CYCGRG 1 cut(s) 322
EcoICRI GAGCTC 2 cut(s) 321, 757
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoO109I RGGNCCY 2 cut(s) 5, 92
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 4 cut(s) 119, 188, 389, 664
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 323, 759
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 5 cut(s) 412, 541, 579, 607, 637
FaiI YATR 8 cut(s) 233, 410, 539, 577, 605, 635, 650, 788
FaqI GGGAC 3 cut(s) 364, 659, 809
FatI CATG 5 cut(s) 408, 537, 575, 603, 633
FokI GGATG 2 cut(s) 122, 672
FriOI GRGCYC 2 cut(s) 323, 759
FspBI CTAG 2 cut(s) 335, 723
GsuI CTGGAG 1 cut(s) 103
HaeIII GGCC 2 cut(s) 442, 664
HapII CCGG 2 cut(s) 657, 778
Hin1II CATG 5 cut(s) 412, 541, 579, 607, 637
HinfI GANTC 4 cut(s) 170, 329, 350, 595
HpaII CCGG 2 cut(s) 657, 778
HphI GGTGA 5 cut(s) 62, 338, 373, 419, 740
Hpy166II GTNNAC 4 cut(s) 215, 261, 364, 625
Hpy188I TCNGA 4 cut(s) 82, 486, 559, 696
Hpy188III TCNNGA 4 cut(s) 538, 587, 644, 826
Hpy8I GTNNAC 4 cut(s) 215, 261, 364, 625
HpyAV CCTTC 7 cut(s) 28, 40, 79, 83, 208, 453, 670
HpyCH4III ACNGT 2 cut(s) 269, 376
HpyCH4V TGCA 4 cut(s) 139, 286, 436, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 39
Hsp92II CATG 5 cut(s) 412, 541, 579, 607, 637
Kzo9I GATC 4 cut(s) 289, 583, 828, 832
MaeI CTAG 2 cut(s) 335, 723
MaeIII GTNAC 5 cut(s) 330, 337, 385, 569, 652
MalI GATC 4 cut(s) 291, 585, 830, 834
MboI GATC 4 cut(s) 289, 583, 828, 832
MboII GAAGA 6 cut(s) 37, 55, 239, 324, 425, 604
MfeI CAATTG 2 cut(s) 342, 505
MhlI GDGCHC 2 cut(s) 323, 759
MlsI TGGCCA 1 cut(s) 664
MluCI AATT 6 cut(s) 113, 126, 342, 479, 505, 703
MluNI TGGCCA 1 cut(s) 664
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 6 cut(s) 8, 46, 413, 488, 630, 719
Mox20I TGGCCA 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 197
MscI TGGCCA 1 cut(s) 664
MseI TTAA 4 cut(s) 129, 180, 308, 471
MslI CAYNNNNRTG 1 cut(s) 539
Msp20I TGGCCA 1 cut(s) 664
MspCI CTTAAG 1 cut(s) 307
MspI CCGG 2 cut(s) 657, 778
MspR9I CCNGG 5 cut(s) 121, 190, 391, 666, 779
MunI CAATTG 2 cut(s) 342, 505
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 4 cut(s) 121, 190, 391, 666
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 779
NcoI CCATGG 1 cut(s) 408
NdeII GATC 4 cut(s) 289, 583, 828, 832
NlaIII CATG 5 cut(s) 412, 541, 579, 607, 637
NlaIV GGNNCC 2 cut(s) 6, 627
NmuCI GTSAC 3 cut(s) 330, 337, 385
NspV TTCGAA 1 cut(s) 701
PagI TCATGA 1 cut(s) 537
PaqCI CACCTGC 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 3 cut(s) 170, 350, 595
PfoI TCCNGGA 3 cut(s) 119, 188, 777
Ple19I CGATCG 1 cut(s) 835
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 2 cut(s) 5, 92
PshBI ATTAAT 1 cut(s) 129
Psp124BI GAGCTC 2 cut(s) 323, 759
Psp5II RGGWCCY 2 cut(s) 5, 92
Psp6I CCWGG 4 cut(s) 119, 188, 389, 664
PspGI CCWGG 4 cut(s) 119, 188, 389, 664
PspN4I GGNNCC 2 cut(s) 6, 627
PspPI GGNCC 3 cut(s) 5, 92, 625
PspPPI RGGWCCY 2 cut(s) 5, 92
PvuI CGATCG 1 cut(s) 835
RseI CAYNNNNRTG 1 cut(s) 539
SacI GAGCTC 2 cut(s) 323, 759
SaqAI TTAA 4 cut(s) 129, 180, 308, 471
Sau3AI GATC 4 cut(s) 289, 583, 828, 832
Sau96I GGNCC 3 cut(s) 5, 92, 625
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 5 cut(s) 121, 190, 391, 666, 779
SduI GDGCHC 2 cut(s) 323, 759
SfcI CTRYAG 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 701
SinI GGWCC 3 cut(s) 5, 92, 625
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 334
Sse9I AATT 6 cut(s) 113, 126, 342, 479, 505, 703
SsiI CCGC 1 cut(s) 513
SspMI CTAG 2 cut(s) 335, 723
SstI GAGCTC 2 cut(s) 323, 759
StyD4I CCNGG 5 cut(s) 119, 188, 389, 664, 777
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 269, 376
TaqI TCGA 5 cut(s) 201, 402, 582, 701, 831
TasI AATT 6 cut(s) 113, 126, 342, 479, 505, 703
TfiI GAWTC 3 cut(s) 170, 350, 595
Tru1I TTAA 4 cut(s) 129, 180, 308, 471
Tru9I TTAA 4 cut(s) 129, 180, 308, 471
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 3 cut(s) 330, 337, 385
Tsp45I GTSAC 3 cut(s) 330, 337, 385
TspDTI ATGAA 2 cut(s) 126, 689
TspRI CASTG 1 cut(s) 454
Vha464I CTTAAG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 5, 92, 625
VspI ATTAAT 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 3 cut(s) 113, 479, 703
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 2 cut(s) 335, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.