pycom12g06170

strictosidine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012570
Physical Location & Seq
Forward (+)
6244247 .. 6244680
434 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 378 bp
ATGAGGCATGAACAGGTACAAGCATTTTATGAAGGATTAAATGATTCGAATAAAGCCATTGTGGATTCCGCTTGCAATGGCATGTTAATGAAGATGAGTGACGAGGAAGCGACTGGAATGTACGAGGAACTTGCAGAGAACTCGCAACAATTTAATACACGGGGACGTCAGGCTAAAAGGGGCGCATATGCAATAAATGCTAATGATGGTATACAAGCTGAGATGGCTGCCATGAAATATTGCAGCACTAATCAAGCCTTATCGCCTCAAAGTTCTCAGAAAGCTTCTACGTCTGATGTATGTGCAATCTGTTCTAAAAATGATCGATGCTACAAATTTGTGTCCGATGACTTCATTTTCGGATCAAGAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 RVT_1 Retrotrans_gag rve

Protein Analysis

126

Amino Acids

13.78

Weight (kDa)

5.1

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 211
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 370
AcsI RAATTY 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 2 cut(s) 18, 122
AluBI AGCT 2 cut(s) 218, 284
AluI AGCT 2 cut(s) 218, 284
AlwI GGATC 1 cut(s) 370
ApeKI GCWGC 2 cut(s) 227, 243
ApoI RAATTY 1 cut(s) 335
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspLEI GCGC 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 47
BbvI GCAGC 2 cut(s) 214, 255
BccI CCATC 2 cut(s) 200, 217
BcgI CGANNNNNNTGC 4 cut(s) 113, 123, 147, 157
BisI GCNGC 2 cut(s) 228, 244
BlsI GCNGC 2 cut(s) 229, 245
BmsI GCATC 1 cut(s) 317
Bpu14I TTCGAA 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 82
BseCI ATCGAT 1 cut(s) 325
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 210, 290
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 2 cut(s) 214, 255
BshVI ATCGAT 1 cut(s) 325
BslFI GGGAC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 47
Bsp143I GATC 2 cut(s) 322, 362
BspACI CCGC 1 cut(s) 69
BspCNI CTCAG 2 cut(s) 211, 289
BspDI ATCGAT 1 cut(s) 325
BspPI GGATC 1 cut(s) 370
BspT104I TTCGAA 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 82
BsrI ACTGG 1 cut(s) 118
BssMI GATC 2 cut(s) 322, 362
BssNAI GTATAC 1 cut(s) 212
BssNI GRCGYC 1 cut(s) 166
Bst1107I GTATAC 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 166
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 219, 276
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 2 cut(s) 325, 365
BstMBI GATC 2 cut(s) 322, 362
BstMWI GCNNNNNNNGC 2 cut(s) 197, 224
BstNSI RCATGY 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 214, 255
BstZ17I GTATAC 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 325
BsuTUI ATCGAT 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 325
Csp6I GTAC 2 cut(s) 17, 121
CviAII CATG 3 cut(s) 8, 82, 232
CviJI RGCY 6 cut(s) 56, 173, 218, 227, 257, 284
CviKI_1 RGCY 6 cut(s) 56, 173, 218, 227, 257, 284
CviQI GTAC 2 cut(s) 17, 121
DdeI CTNAG 2 cut(s) 219, 276
DpnI GATC 2 cut(s) 324, 364
DpnII GATC 2 cut(s) 322, 362
FaeI CATG 3 cut(s) 11, 85, 235
FaiI YATR 8 cut(s) 9, 30, 83, 187, 189, 212, 233, 301
FaqI GGGAC 1 cut(s) 177
FatI CATG 3 cut(s) 7, 81, 231
FauNDI CATATG 1 cut(s) 187
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 228, 244
Fsp4HI GCNGC 2 cut(s) 228, 244
GlaI GCGC 1 cut(s) 184
GluI GCNGC 2 cut(s) 228, 244
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 3 cut(s) 11, 85, 235
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 2 cut(s) 44, 65
Hpy166II GTNNAC 1 cut(s) 212
Hpy188I TCNGA 4 cut(s) 279, 295, 346, 362
Hpy188III TCNNGA 1 cut(s) 366
Hpy8I GTNNAC 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 26
HpyCH4IV ACGT 2 cut(s) 166, 290
HpyCH4V TGCA 5 cut(s) 75, 134, 191, 243, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 224
HpyF3I CTNAG 2 cut(s) 219, 276
HpySE526I ACGT 2 cut(s) 166, 290
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 3 cut(s) 11, 85, 235
HspAI GCGC 1 cut(s) 183
Kzo9I GATC 2 cut(s) 322, 362
LpnPI CCDG 2 cut(s) 99, 155
Lsp1109I GCAGC 2 cut(s) 214, 255
LweI GCATC 1 cut(s) 317
MaeII ACGT 2 cut(s) 166, 290
MaeIII GTNAC 1 cut(s) 98
MalI GATC 2 cut(s) 324, 364
MboI GATC 2 cut(s) 322, 362
MboII GAAGA 1 cut(s) 103
MluCI AATT 2 cut(s) 149, 335
MnlI CCTC 3 cut(s) 97, 118, 276
MseI TTAA 4 cut(s) 38, 86, 153, 376
MslI CAYNNNNRTG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 197, 224
NdeI CATATG 1 cut(s) 187
NdeII GATC 2 cut(s) 322, 362
NlaIII CATG 3 cut(s) 11, 85, 235
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 1 cut(s) 85
NspV TTCGAA 1 cut(s) 47
PfeI GAWTC 2 cut(s) 44, 65
PkrI GCNGC 2 cut(s) 229, 245
RsaI GTAC 2 cut(s) 18, 122
RsaNI GTAC 2 cut(s) 17, 121
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 4 cut(s) 38, 86, 153, 376
SatI GCNGC 2 cut(s) 228, 244
Sau3AI GATC 2 cut(s) 322, 362
SetI ASST 5 cut(s) 18, 169, 220, 286, 293
SfaNI GCATC 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 86
Sse9I AATT 2 cut(s) 149, 335
SsiI CCGC 1 cut(s) 69
SspI AATATT 1 cut(s) 239
TaiI ACGT 2 cut(s) 169, 293
TaqI TCGA 2 cut(s) 47, 325
TasI AATT 2 cut(s) 149, 335
TfiI GAWTC 2 cut(s) 44, 65
Tru1I TTAA 4 cut(s) 38, 86, 153, 376
Tru9I TTAA 4 cut(s) 38, 86, 153, 376
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 2 cut(s) 227, 243
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 5 cut(s) 24, 45, 104, 248, 343
XapI RAATTY 1 cut(s) 335
XceI RCATGY 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 211
ZraI GACGTC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.