pycom12g06700

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002829
Physical Location & Seq
Reverse (-)
6965210 .. 6968432
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGGAGGTCGCGGACCGAGAGAATTACTTAGGAGAGGGTGTTATTGAGGCCTATTCACAAAAATCCAATGACATTGATGAACATGAAGATCACTATGCTTCTGCGTCTTCTTCCAAGTCGCAATTCAGGAGAGTGGCATTGAAGGCTCGCTGGAGTGATGAGACGGGCATGGCTGAGGTTGTAGATAAAAGGGGTACAATTTGGAGGACCACGGGAATTGTTCGTAGTAGCAAGCTCTATTTCTCCATTGAGGAAGTTTTGTTTTTGATTGAAATAGGGGCTTTGCTTCTTATGGATGATGGTGATATTAGCCTTTCCTTGGAAGACATGCATGTGAAGATTTCAAATGGAAAGCACGGGTGCAGTTGGGAGGAATTTCAAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGACATGGCATCCCCTGGTCTATGAAGATTGTGAAGAGTAATCATGAATCTGTTTCTTCTTACGGTTGCCGTGACAATGATGAGGCAGTGGACTTGGATTCAGAAGAGTCTAGGTCTGTCTTTGGATTGTTCAATGAGATGCAAATTGATGAAGCAAGGCTGGTTTTTGATGTTTATCTCCCAAACAGCAACTTCAGGAAGTCTTCTCCTGGTGACCCAAGTTTTGTGCTCTGCTTTACCAGGGGTCATCCACCATCCAAAGCAGACCTAGAAGCCCTCGAGAGACGATGCAGGAACATTCCCGTGAAGGTTTGTCATGTGGAGCAGGGGGGTGTGAGTTTATTTTCCTTTGACAAGGTGGAACTTCCAATTCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

267

Amino Acids

30.06

Weight (kDa)

5.18

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 39 - 97 4.9e-12 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 11
AciI CCGC 1 cut(s) 11
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 598
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 5 cut(s) 142, 272, 345, 380, 553
AjnI CCWGG 3 cut(s) 434, 628, 659
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 2 cut(s) 235, 383
AluI AGCT 2 cut(s) 235, 383
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 2 cut(s) 155, 697
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 1 cut(s) 48
ApoI RAATTY 1 cut(s) 374
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AspS9I GGNCC 2 cut(s) 13, 207
AsuHPI GGTGA 2 cut(s) 314, 644
AvaI CYCGRG 1 cut(s) 698
AvaII GGWCC 2 cut(s) 13, 207
BbsI GAAGAC 3 cut(s) 99, 330, 615
Bbv12I GWGCWC 1 cut(s) 651
BbvCI CCTCAGC 1 cut(s) 174
BccI CCATC 2 cut(s) 293, 682
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 3 cut(s) 436, 630, 661
BcoDI GTCTC 2 cut(s) 155, 697
BfaI CTAG 2 cut(s) 531, 689
Bme1390I CCNGG 3 cut(s) 436, 630, 661
Bme18I GGWCC 2 cut(s) 13, 207
BmeT110I CYCGRG 1 cut(s) 698
BmgT120I GGNCC 2 cut(s) 13, 207
BmrFI CCNGG 3 cut(s) 436, 630, 661
BmsI GCATC 3 cut(s) 438, 549, 698
BpiI GAAGAC 3 cut(s) 99, 330, 615
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 594
BsaJI CCNNGG 4 cut(s) 210, 318, 434, 660
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 3 cut(s) 436, 630, 661
BseDI CCNNGG 4 cut(s) 210, 318, 434, 660
BseGI GGATG 4 cut(s) 301, 429, 667, 674
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 165
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 651
BsiHKCI CYCGRG 1 cut(s) 698
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 155, 697
BsmBI CGTCTC 2 cut(s) 155, 697
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 1 cut(s) 463
BssECI CCNNGG 4 cut(s) 210, 318, 434, 660
BssMI GATC 1 cut(s) 88
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 3 cut(s) 436, 630, 661
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 2 cut(s) 449, 519
BstC8I GCNNGC 2 cut(s) 148, 233
BstDEI CTNAG 2 cut(s) 28, 174
BstDSI CCRYGG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 632
BstF5I GGATG 4 cut(s) 301, 429, 667, 674
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 2 cut(s) 155, 697
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 3 cut(s) 436, 630, 661
BstNSI RCATGY 2 cut(s) 331, 335
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 434, 628, 659
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 3 cut(s) 99, 330, 615
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 4 cut(s) 301, 429, 667, 674
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 148, 233
CciI TCATGA 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 13, 207
CpoI CGGWCCG 1 cut(s) 13
CseI GACGC 1 cut(s) 93
Csp6I GTAC 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CspI CGGWCCG 1 cut(s) 13
CviAII CATG 7 cut(s) 83, 169, 328, 332, 425, 464, 737
CviJI RGCY 9 cut(s) 50, 146, 173, 235, 281, 312, 383, 580, 695
CviKI_1 RGCY 9 cut(s) 50, 146, 173, 235, 281, 312, 383, 580, 695
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 2 cut(s) 28, 174
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 449, 519
EarI CTCTTC 2 cut(s) 449, 519
Eco130I CCWWGG 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 13, 207
Eco57I CTGAAG 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 698
Eco91I GGTNACC 1 cut(s) 632
EcoO65I GGTNACC 1 cut(s) 632
EcoRII CCWGG 3 cut(s) 434, 628, 659
EcoT14I CCWWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 2 cut(s) 155, 697
FaeI CATG 7 cut(s) 86, 172, 331, 335, 428, 467, 740
FatI CATG 7 cut(s) 82, 168, 327, 331, 424, 463, 736
FokI GGATG 4 cut(s) 308, 416, 654, 661
FspBI CTAG 2 cut(s) 531, 689
GsuI CTGGAG 1 cut(s) 172
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 1 cut(s) 93
Hin1II CATG 7 cut(s) 86, 172, 331, 335, 428, 467, 740
HindIII AAGCTT 1 cut(s) 381
HinfI GANTC 3 cut(s) 467, 518, 527
HphI GGTGA 2 cut(s) 314, 644
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 1 cut(s) 523
Hpy188III TCNNGA 4 cut(s) 127, 464, 616, 700
Hpy8I GTNNAC 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 136, 721
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 331, 363, 562, 711
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 28, 174
Hsp92II CATG 7 cut(s) 86, 172, 331, 335, 428, 467, 740
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 742
LweI GCATC 3 cut(s) 438, 549, 698
MaeI CTAG 2 cut(s) 531, 689
MaeIII GTNAC 3 cut(s) 407, 491, 632
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 7 cut(s) 22, 122, 198, 216, 374, 564, 789
MlyI GAGTC 1 cut(s) 536
MnlI CCTC 8 cut(s) 28, 40, 169, 198, 244, 364, 496, 707
Mph1103I ATGCAT 1 cut(s) 333
MslI CAYNNNNRTG 2 cut(s) 332, 722
MspR9I CCNGG 3 cut(s) 436, 630, 661
MvaI CCWGG 3 cut(s) 436, 630, 661
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeII GATC 1 cut(s) 88
NlaIII CATG 7 cut(s) 86, 172, 331, 335, 428, 467, 740
NmuCI GTSAC 2 cut(s) 491, 632
NsiI ATGCAT 1 cut(s) 333
NspI RCATGY 2 cut(s) 331, 335
PaeR7I CTCGAG 1 cut(s) 698
PagI TCATGA 1 cut(s) 463
PceI AGGCCT 1 cut(s) 50
PfeI GAWTC 2 cut(s) 467, 518
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
Psp6I CCWGG 3 cut(s) 434, 628, 659
PspEI GGTNACC 1 cut(s) 632
PspGI CCWGG 3 cut(s) 434, 628, 659
PspPI GGNCC 2 cut(s) 13, 207
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 2 cut(s) 332, 722
Rsr2I CGGWCCG 1 cut(s) 13
RsrII CGGWCCG 1 cut(s) 13
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 2 cut(s) 13, 207
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 3 cut(s) 436, 630, 661
SduI GDGCHC 1 cut(s) 651
SetI ASST 8 cut(s) 9, 180, 237, 385, 536, 690, 732, 780
SfaNI GCATC 3 cut(s) 438, 549, 698
Sfr274I CTCGAG 1 cut(s) 698
SinI GGWCC 2 cut(s) 13, 207
SlaI CTCGAG 1 cut(s) 698
SmiMI CAYNNNNRTG 2 cut(s) 332, 722
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
Sse9I AATT 7 cut(s) 22, 122, 198, 216, 374, 564, 789
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 1 cut(s) 11
SspMI CTAG 2 cut(s) 531, 689
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 434, 628, 659
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 1 cut(s) 699
TaqII GACCGA 1 cut(s) 30
TasI AATT 7 cut(s) 22, 122, 198, 216, 374, 564, 789
TfiI GAWTC 2 cut(s) 467, 518
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 2 cut(s) 491, 632
Tsp45I GTSAC 2 cut(s) 491, 632
TspDTI ATGAA 5 cut(s) 93, 99, 458, 480, 585
TspRI CASTG 1 cut(s) 513
VpaK11BI GGWCC 2 cut(s) 13, 207
XapI RAATTY 1 cut(s) 374
XceI RCATGY 2 cut(s) 331, 335
XhoI CTCGAG 1 cut(s) 698
XspI CTAG 2 cut(s) 531, 689
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.