pycom12g11550

adenosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002092
Physical Location & Seq
Reverse (-)
13766449 .. 13768791
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGGCGTCCGAGGGAATTCTGTTGGGGATGGGCAACCCACTGCTAGACATCTCCGCCGTCGTCGACGAGGAGTTCTTGCAGAAATATGACCTCAAGCCCAACAATGCGATTCTTGCAGAGGACAAGCACCTGCCCATGTACGACGAAATGTCTTCCGAGTACAACGTGGAGTACATTGCTGGAGGTGCTACTCAGAATTCCATTCGAGTTGCTCAGTGGATGCTTCAAGTACCTGGTGCAACAAGTTATATGGGTTCCATTGGAAAGGACAAGTATGGTGAAGAGATGAAGAAGAACTCAAAACTTGCTGGTGTTAATGTTCACTATTATGAGGATGAGACTGCCCCAACGGGAACTTGTGCTGTCTGTGTTGTTGGTGGGGAAAGGTCCCTTATTGCCAATTTGTCAGCTGCTAACTGCTACAAATTTGAGCATTTAAAGAAACCCGAAAATTGGGCACTGGTTGAGAAGGCCAAGTACATCTACATTGCTGGTTTTTTTCTAACTGTATCCCCGGACTCCATCCAACTTGTTGCTGAACATGCTGCTGCAAATAACAAGGTTTTCTCAATGAACCTTTCTGCTCCATTTATCTGTGAGTTTTTCAAGGATGCTCAGGAGAAAGCTTTGCCGTATGTGGACTACATTTTTGGAAATGAGACGGAAGCAAGAACCTTCTCAAAAGTTCGTGGTTGGGAGACTGATGATGTCGAGCAAATAGCTCTAAAGATCGATCCCAATGGCCCAAAGCGTCAGGGACACACAAGAGGATTACTGTTATTACTCAAGGCGCAGATCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004001 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006166 GO:0006167 GO:0006169 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0030054 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0043094 GO:0043101 GO:0043167 GO:0043169 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046033 GO:0046085 GO:0046086 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0046683 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0080094 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

268

Amino Acids

29.62

Weight (kDa)

5.15

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 26 - 249 2.2e-45 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 63
AciI CCGC 1 cut(s) 54
AclWI GGATC 2 cut(s) 728, 790
AcsI RAATTY 3 cut(s) 15, 196, 425
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 140, 161, 173, 231, 479
AfiI CCNNNNNNNGG 1 cut(s) 453
AgsI TTSAA 2 cut(s) 227, 607
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 3 cut(s) 410, 626, 722
AluI AGCT 3 cut(s) 410, 626, 722
Alw26I GTCTC 3 cut(s) 332, 653, 692
AlwI GGATC 2 cut(s) 728, 790
AlwNI CAGNNNCTG 1 cut(s) 799
AoxI GGCC 2 cut(s) 471, 742
ApeKI GCWGC 3 cut(s) 410, 545, 548
ApoI RAATTY 3 cut(s) 15, 196, 425
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 2 cut(s) 387, 743
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 460
BarI GAAGNNNNNNTAC 2 cut(s) 461, 493
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 397, 532, 535
BccI CCATC 2 cut(s) 22, 530
BceAI ACGGC 2 cut(s) 41, 616
BciT130I CCWGG 1 cut(s) 234
BciVI GTATCC 1 cut(s) 520
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 3 cut(s) 332, 653, 692
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 138
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 3 cut(s) 411, 546, 549
BlsI GCNGC 3 cut(s) 412, 547, 550
Bme1390I CCNGG 2 cut(s) 234, 515
Bme18I GGWCC 1 cut(s) 387
BmeRI GACNNNNNGTC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 387, 743
BmiI GGNNCC 2 cut(s) 256, 389
BmrFI CCNGG 2 cut(s) 234, 515
BmsI GCATC 2 cut(s) 210, 601
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 615
BpuEI CTTGAG 2 cut(s) 77, 770
BpuMI CCSGG 1 cut(s) 515
Bsa29I ATCGAT 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 9, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 465
Bse3DI GCAATG 2 cut(s) 174, 486
BseBI CCWGG 1 cut(s) 234
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 9, 513
BseGI GGATG 5 cut(s) 33, 225, 340, 522, 616
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMI GCAATG 2 cut(s) 174, 486
BseMII CTCAG 3 cut(s) 206, 227, 629
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 397, 532, 535
BshFI GGCC 2 cut(s) 473, 744
BshVI ATCGAT 1 cut(s) 732
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 2 cut(s) 373, 771
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 3 cut(s) 332, 653, 692
BsmBI CGTCTC 1 cut(s) 653
BsmFI GGGAC 2 cut(s) 373, 771
BsnI GGCC 2 cut(s) 473, 744
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 729, 733, 795
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 473, 744
BspCNI CTCAG 3 cut(s) 205, 226, 628
BspDI ATCGAT 1 cut(s) 732
BspLI GGNNCC 2 cut(s) 256, 389
BspMI ACCTGC 1 cut(s) 138
BspPI GGATC 2 cut(s) 728, 790
BsrDI GCAATG 2 cut(s) 174, 486
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 2 cut(s) 9, 513
BssMI GATC 3 cut(s) 729, 733, 795
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 508, 777
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 3 cut(s) 192, 213, 615
BstF5I GGATG 5 cut(s) 33, 225, 340, 522, 616
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 3 cut(s) 732, 736, 798
BstMAI GTCTC 3 cut(s) 332, 653, 692
BstMBI GATC 3 cut(s) 729, 733, 795
BstMWI GCNNNNNNNGC 3 cut(s) 113, 185, 542
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 2 cut(s) 232, 513
BstSFI CTRYAG 1 cut(s) 800
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 397, 532, 535
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 795
BstYI RGATCY 1 cut(s) 795
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 2 cut(s) 473, 744
BsuTUI ATCGAT 1 cut(s) 732
BtsCI GGATG 5 cut(s) 33, 225, 340, 522, 616
BtsI GCAGTG 1 cut(s) 38
BtsIMutI CAGTG 3 cut(s) 38, 221, 458
BveI ACCTGC 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 387, 743
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 1 cut(s) 740
CsiI ACCWGGT 1 cut(s) 232
Csp6I GTAC 5 cut(s) 139, 160, 172, 230, 478
CviAII CATG 2 cut(s) 136, 542
CviJI RGCY 6 cut(s) 97, 410, 473, 626, 722, 744
CviKI_1 RGCY 6 cut(s) 97, 410, 473, 626, 722, 744
CviQI GTAC 5 cut(s) 139, 160, 172, 230, 478
DdeI CTNAG 3 cut(s) 192, 213, 615
DpnI GATC 3 cut(s) 731, 735, 797
DpnII GATC 3 cut(s) 729, 733, 795
DraI TTTAAA 1 cut(s) 438
DriI GACNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 387
EcoRI GAATTC 2 cut(s) 15, 196
EcoRII CCWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 653
FaeI CATG 2 cut(s) 139, 545
FaiI YATR 8 cut(s) 87, 137, 249, 251, 276, 330, 543, 636
FaqI GGGAC 2 cut(s) 373, 771
FatI CATG 2 cut(s) 135, 541
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 411, 546, 549
FokI GGATG 5 cut(s) 40, 232, 347, 509, 623
Fsp4HI GCNGC 3 cut(s) 411, 546, 549
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 792
GluI GCNGC 3 cut(s) 411, 546, 549
GsuI CTGGAG 1 cut(s) 201
HaeIII GGCC 2 cut(s) 473, 744
HapII CCGG 1 cut(s) 515
HgaI GACGC 1 cut(s) 740
HhaI GCGC 1 cut(s) 793
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 139, 545
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 2 cut(s) 109, 518
HpaII CCGG 1 cut(s) 515
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 64, 322, 640
Hpy188I TCNGA 3 cut(s) 10, 157, 195
Hpy188III TCNNGA 1 cut(s) 617
Hpy8I GTNNAC 3 cut(s) 64, 322, 640
Hpy99I CGWCG 4 cut(s) 62, 65, 68, 146
HpyAV CCTTC 2 cut(s) 463, 685
HpyCH4III ACNGT 2 cut(s) 508, 777
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 4 cut(s) 79, 116, 239, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 185, 542
HpyF3I CTNAG 3 cut(s) 192, 213, 615
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 139, 545
HspAI GCGC 1 cut(s) 791
Kzo9I GATC 3 cut(s) 729, 733, 795
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 3 cut(s) 397, 532, 535
LweI GCATC 2 cut(s) 210, 601
MabI ACCWGGT 1 cut(s) 232
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 165
MalI GATC 3 cut(s) 731, 735, 797
MboI GATC 3 cut(s) 729, 733, 795
MboII GAAGA 4 cut(s) 144, 293, 301, 304
MflI RGATCY 1 cut(s) 795
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 5 cut(s) 15, 196, 400, 425, 451
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 7 cut(s) 4, 61, 101, 112, 176, 325, 761
MseI TTAA 2 cut(s) 315, 437
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 234, 515
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 113, 185, 542
NciI CCSGG 1 cut(s) 515
NdeII GATC 3 cut(s) 729, 733, 795
NlaIII CATG 2 cut(s) 139, 545
NlaIV GGNNCC 2 cut(s) 256, 389
NspI RCATGY 1 cut(s) 545
PaqCI CACCTGC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 412, 547, 550
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PpuMI RGGWCCY 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 256, 389
PspPI GGNCC 2 cut(s) 387, 743
PspPPI RGGWCCY 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 795
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 5 cut(s) 140, 161, 173, 231, 479
RsaNI GTAC 5 cut(s) 139, 160, 172, 230, 478
RseI CAYNNNNRTG 1 cut(s) 327
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 315, 437
SatI GCNGC 3 cut(s) 411, 546, 549
Sau3AI GATC 3 cut(s) 729, 733, 795
Sau96I GGNCC 2 cut(s) 387, 743
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 234, 515
SduI GDGCHC 1 cut(s) 460
SexAI ACCWGGT 1 cut(s) 232
SfaNI GCATC 2 cut(s) 210, 601
SfcI CTRYAG 1 cut(s) 800
SgrDI CGTCGACG 1 cut(s) 62
SinI GGWCC 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 92, 785
SmoI CTYRAG 2 cut(s) 92, 785
Sse9I AATT 5 cut(s) 15, 196, 400, 425, 451
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 232, 513
TaaI ACNGT 2 cut(s) 508, 777
TaiI ACGT 1 cut(s) 168
TaqI TCGA 4 cut(s) 63, 205, 711, 732
TasI AATT 5 cut(s) 15, 196, 400, 425, 451
TatI WGTACW 3 cut(s) 159, 171, 477
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 315, 437
Tru9I TTAA 2 cut(s) 315, 437
TscAI CASTG 3 cut(s) 45, 221, 465
TseI GCWGC 3 cut(s) 410, 545, 548
TspDTI ATGAA 2 cut(s) 302, 587
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 3 cut(s) 45, 221, 465
VpaK11BI GGWCC 1 cut(s) 387
XapI RAATTY 3 cut(s) 15, 196, 425
XceI RCATGY 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.