pycom12g18020

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003304
Physical Location & Seq
Reverse (-)
20311196 .. 20314132
2937 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGGAGAGGGTTTTGATGGCGGTGCATGTGGCGAATCAAGTCGGAGGGGAATCGTTACTTGGGACGATCAAGATCGCGGTGCTTCCCATAGCAAAGGTTTTTACTGTGTGCTCCTTGGGGCTTCTAATGGCTTCCAAGTATGTCAACATCTTCCCAGCCAGTGGAAGAAAACTCTTGAATGGGCTGGTCTTCTCGCTCTTGCTTCCATGTTTGATATTTTCTCAGCTTGGACAAGCCATCACCTTACAGAAAATGCTCGAGTGGTGGTTTATTCCCGTGAATGTCGTGATTGGTAGCACTACAGGCTCCATAATAGGTTATATCGTTGCATCCATTGTCCGACCACCGTACCCCTTCTTCAAGTTTACAATTGTACAAATTGGAATAGGAAACATCGGGAATGTGCCACTGGTTCTAATTGCGGCTTTATGTAGAGACAAATCGAACCCTTTCGGTGATACATGTAAAACAGATGGGACTGCCTATATTTCATTTGGCCAGTGGGTTGGTGCAATCATTCTATACACGTATGTATTTCAAATGCTGTCCCCCCCTCCAGAAGGTACCTTTGACATTGAGGAGAAAGATCTCCCAATCAAAAGCCCTCGAAACAGCACGAGCACGACACCTGACCAAATTCCATTGCTTATAAATGATGAAAATGATGAAGAGCCAACACGTCAAGAAGAGGTTGCAGAAACTAACTCAAATGATTCAGATAAACCTAAGATTACAAAGTTCTTCGTATTTATATACGAAAAGTTGAAGCTCAAACAAGTTCTCCAACCACCTATAATAGCTTCTATCCTGGCCATGGTACTTGGAGCGATACCATTTTTAAAGAAATCGATCTTTACATCTGATGGTCCACTTTTCTTCTTCACCGATAGCTGCATTATCCTTGGGGAAGCCATGATTCCGTGCATTCTGTTGGCATTAGGTGGCAACCTTATTGATGGTCCGGGAAGTTCAAAACTTGGTCTACGGACAACTGCTGCAATTATATTTGCACGACTACTCTTGGTGCCCCCCGTAGGACTTGGCATTGTAATGTTAGCCGATAAGCTCGGCTTCCTCCCTGCCGATGACAAAATGTTTCGATTTGTCCTGCTGCTCCAGAACACAATGCCTACATCTGTCCTTGCTGGTGCTGTTGCAAATTTAAGAGGCTGCGGAAGAGAGGCAGCAGCCGTCCTGTTCTGGGTGCACATATTCGCCATCTTCTCCATGGCTGGGTGGATCGTCTTGTACCTCAACCTACTCTTCTGA

Protein Analysis

423

Amino Acids

45.94

Weight (kDa)

7.51

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 27 - 414 4.8e-96 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 563, 1024
AccB7I CCANNNNNTGG 1 cut(s) 161
AccI GTMKAC 1 cut(s) 982
AccII CGCG 1 cut(s) 77
AciI CCGC 4 cut(s) 20, 77, 422, 1173
AclWI GGATC 1 cut(s) 1247
AcoI YGGCCR 2 cut(s) 496, 810
AcsI RAATTY 2 cut(s) 636, 1159
AfaI GTAC 5 cut(s) 350, 375, 565, 819, 1250
AfiI CCNNNNNNNGG 6 cut(s) 161, 560, 814, 1034, 1201, 1233
AflIII ACRYGT 3 cut(s) 461, 525, 677
AgsI TTSAA 5 cut(s) 178, 361, 539, 766, 972
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 1 cut(s) 807
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 5 cut(s) 226, 769, 800, 891, 1066
AluI AGCT 5 cut(s) 226, 769, 800, 891, 1066
Alw21I GWGCWC 3 cut(s) 113, 623, 1209
Alw26I GTCTC 1 cut(s) 429
Alw44I GTGCAC 1 cut(s) 1205
AlwI GGATC 1 cut(s) 1247
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 2 cut(s) 496, 810
ApaLI GTGCAC 1 cut(s) 1205
ApeKI GCWGC 6 cut(s) 891, 995, 1111, 1170, 1184, 1187
ApoI RAATTY 2 cut(s) 636, 1159
Asp700I GAANNNNTTC 1 cut(s) 449
Asp718I GGTACC 1 cut(s) 563
AspS9I GGNCC 2 cut(s) 866, 959
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 3 cut(s) 232, 467, 874
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 866, 959
BaeGI GKGCMC 2 cut(s) 1029, 1209
BaeI ACNNNNGTAYC 2 cut(s) 332, 365
BalI TGGCCA 2 cut(s) 498, 812
BanI GGYRCC 2 cut(s) 563, 1024
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 3 cut(s) 113, 623, 1209
BbvI GCAGC 6 cut(s) 878, 982, 1098, 1157, 1196, 1199
BccI CCATC 6 cut(s) 10, 245, 467, 857, 950, 1226
BceAI ACGGC 1 cut(s) 1175
BciT130I CCWGG 1 cut(s) 809
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 300
BglII AGATCT 1 cut(s) 586
BisI GCNGC 7 cut(s) 423, 892, 996, 1112, 1171, 1185, 1188
BlsI GCNGC 7 cut(s) 424, 893, 997, 1113, 1172, 1186, 1189
Bme1390I CCNGG 2 cut(s) 809, 963
Bme18I GGWCC 2 cut(s) 866, 959
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 2 cut(s) 866, 959
BmiI GGNNCC 3 cut(s) 307, 565, 1026
BmrFI CCNGG 2 cut(s) 809, 963
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 181
BpmI CTGGAG 2 cut(s) 540, 1100
BpuMI CCSGG 1 cut(s) 963
Bsa29I ATCGAT 1 cut(s) 848
BsaAI YACGTR 1 cut(s) 528
BsaBI GATNNNNATC 1 cut(s) 71
BsaJI CCNNGG 4 cut(s) 114, 813, 901, 1227
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 560, 814, 1034, 1201, 1233
Bse1I ACTGG 3 cut(s) 159, 414, 499
Bse3DI GCAATG 1 cut(s) 641
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 1 cut(s) 809
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 4 cut(s) 114, 813, 901, 1227
BseGI GGATG 1 cut(s) 329
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 6 cut(s) 161, 560, 814, 1034, 1201, 1233
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 3 cut(s) 159, 414, 499
BseRI GAGGAG 1 cut(s) 593
BseSI GKGCMC 2 cut(s) 1029, 1209
BseXI GCAGC 6 cut(s) 878, 982, 1098, 1157, 1196, 1199
BseYI CCCAGC 2 cut(s) 154, 1232
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 498, 812
BshNI GGYRCC 2 cut(s) 563, 1024
BshVI ATCGAT 1 cut(s) 848
BsiHKAI GWGCWC 3 cut(s) 113, 623, 1209
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 962
BslFI GGGAC 3 cut(s) 76, 490, 532
BslI CCNNNNNNNGG 6 cut(s) 161, 560, 814, 1034, 1201, 1233
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 3 cut(s) 76, 490, 532
BsmI GAATGC 1 cut(s) 924
BsnI GGCC 2 cut(s) 498, 812
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 4 cut(s) 113, 623, 1029, 1209
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 5 cut(s) 66, 72, 586, 849, 1239
Bsp19I CCATGG 2 cut(s) 813, 1227
BspACI CCGC 4 cut(s) 20, 77, 422, 1173
BspANI GGCC 2 cut(s) 498, 812
BspCNI CTCAG 1 cut(s) 235
BspDI ATCGAT 1 cut(s) 848
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 3 cut(s) 307, 565, 1026
BspPI GGATC 1 cut(s) 1247
BspQI GCTCTTC 1 cut(s) 663
BspT107I GGYRCC 2 cut(s) 563, 1024
BsrDI GCAATG 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 3 cut(s) 159, 414, 499
BssECI CCNNGG 4 cut(s) 114, 813, 901, 1227
BssMI GATC 5 cut(s) 66, 72, 586, 849, 1239
BssSI CACGAG 1 cut(s) 616
BssT1I CCWWGG 4 cut(s) 114, 813, 901, 1227
Bst2BI CACGAG 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 2 cut(s) 106, 348
Bst6I CTCTTC 3 cut(s) 663, 681, 1171
BstAUI TGTACA 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 222, 726
BstDSI CCRYGG 2 cut(s) 813, 1227
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 329
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 5 cut(s) 69, 75, 589, 852, 1242
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 5 cut(s) 66, 72, 586, 849, 1239
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 2 cut(s) 29, 465
BstSCI CCNGG 2 cut(s) 807, 961
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 1029, 1209
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 6 cut(s) 878, 982, 1098, 1157, 1196, 1199
BstV2I GAAGAC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 586
BstXI CCANNNNNNTGG 1 cut(s) 506
BstYI RGATCY 1 cut(s) 586
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 2 cut(s) 498, 812
BsuTUI ATCGAT 1 cut(s) 848
BtgI CCRYGG 2 cut(s) 813, 1227
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 1 cut(s) 329
BtsIMutI CAGTG 3 cut(s) 166, 407, 506
Cfr13I GGNCC 2 cut(s) 866, 959
ClaI ATCGAT 1 cut(s) 848
Csp6I GTAC 5 cut(s) 349, 374, 564, 818, 1249
CviAII CATG 6 cut(s) 26, 207, 462, 814, 913, 1228
CviQI GTAC 5 cut(s) 349, 374, 564, 818, 1249
DdeI CTNAG 2 cut(s) 222, 726
DpnI GATC 5 cut(s) 68, 74, 588, 851, 1241
DpnII GATC 5 cut(s) 66, 72, 586, 849, 1239
DraI TTTAAA 1 cut(s) 840
EaeI YGGCCR 2 cut(s) 496, 810
Eam1104I CTCTTC 3 cut(s) 663, 681, 1171
EarI CTCTTC 3 cut(s) 663, 681, 1171
Eco130I CCWWGG 4 cut(s) 114, 813, 901, 1227
Eco47I GGWCC 2 cut(s) 866, 959
Eco88I CYCGRG 1 cut(s) 257
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 807
EcoT14I CCWWGG 4 cut(s) 114, 813, 901, 1227
ErhI CCWWGG 4 cut(s) 114, 813, 901, 1227
FaeI CATG 6 cut(s) 29, 210, 465, 817, 916, 1231
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
FaqI GGGAC 3 cut(s) 76, 490, 532
FatI CATG 6 cut(s) 25, 206, 461, 813, 912, 1227
FblI GTMKAC 1 cut(s) 982
Fnu4HI GCNGC 7 cut(s) 423, 892, 996, 1112, 1171, 1185, 1188
FokI GGATG 1 cut(s) 316
Fsp4HI GCNGC 7 cut(s) 423, 892, 996, 1112, 1171, 1185, 1188
GluI GCNGC 7 cut(s) 423, 892, 996, 1112, 1171, 1185, 1188
GsaI CCCAGC 2 cut(s) 158, 1236
GsuI CTGGAG 2 cut(s) 540, 1100
HaeIII GGCC 2 cut(s) 498, 812
HapII CCGG 1 cut(s) 962
Hin1II CATG 6 cut(s) 29, 210, 465, 817, 916, 1231
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 4 cut(s) 34, 50, 713, 916
HpaII CCGG 1 cut(s) 962
HphI GGTGA 3 cut(s) 232, 467, 874
Hpy166II GTNNAC 5 cut(s) 145, 366, 869, 983, 1207
Hpy188I TCNGA 5 cut(s) 44, 341, 718, 862, 1268
Hpy188III TCNNGA 7 cut(s) 70, 175, 286, 397, 557, 683, 1117
Hpy8I GTNNAC 5 cut(s) 145, 366, 869, 983, 1207
HpyAV CCTTC 2 cut(s) 364, 554
HpyCH4III ACNGT 2 cut(s) 106, 348
HpyCH4IV ACGT 2 cut(s) 527, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 2 cut(s) 222, 726
HpySE526I ACGT 2 cut(s) 527, 679
Hsp92II CATG 6 cut(s) 29, 210, 465, 817, 916, 1231
KpnI GGTACC 1 cut(s) 567
Kzo9I GATC 5 cut(s) 66, 72, 586, 849, 1239
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 4 cut(s) 116, 311, 824, 1119
Lsp1109I GCAGC 6 cut(s) 878, 982, 1098, 1157, 1196, 1199
LweI GCATC 1 cut(s) 338
MaeII ACGT 2 cut(s) 527, 679
MaeIII GTNAC 1 cut(s) 54
MalI GATC 5 cut(s) 68, 74, 588, 851, 1241
MboI GATC 5 cut(s) 66, 72, 586, 849, 1239
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 4 cut(s) 113, 623, 1029, 1209
MlsI TGGCCA 2 cut(s) 498, 812
MluCI AATT 6 cut(s) 369, 378, 417, 636, 999, 1159
MluNI TGGCCA 2 cut(s) 498, 812
MmeI TCCRAC 3 cut(s) 22, 364, 808
MnlI CCTC 9 cut(s) 38, 564, 571, 615, 682, 1085, 1160, 1174, 1262
Mox20I TGGCCA 2 cut(s) 498, 812
MroXI GAANNNNTTC 1 cut(s) 449
MscI TGGCCA 2 cut(s) 498, 812
MseI TTAA 2 cut(s) 839, 1163
MslI CAYNNNNRTG 1 cut(s) 1049
Msp20I TGGCCA 2 cut(s) 498, 812
MspI CCGG 1 cut(s) 962
MspR9I CCNGG 2 cut(s) 809, 963
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 924
MvaI CCWGG 1 cut(s) 809
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 963
NcoI CCATGG 2 cut(s) 813, 1227
NdeII GATC 5 cut(s) 66, 72, 586, 849, 1239
NlaIII CATG 6 cut(s) 29, 210, 465, 817, 916, 1231
NlaIV GGNNCC 3 cut(s) 307, 565, 1026
NmeAIII GCCGAG 1 cut(s) 1047
NspI RCATGY 2 cut(s) 29, 465
PaeR7I CTCGAG 1 cut(s) 257
PciI ACATGT 1 cut(s) 461
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 924
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 4 cut(s) 34, 50, 713, 916
PflMI CCANNNNNTGG 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 7 cut(s) 424, 893, 997, 1113, 1172, 1186, 1189
Ppu21I YACGTR 1 cut(s) 528
PscI ACATGT 1 cut(s) 461
PsiI TTATAA 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 807
PspFI CCCAGC 2 cut(s) 154, 1232
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 3 cut(s) 307, 565, 1026
PspPI GGNCC 2 cut(s) 866, 959
PspXI VCTCGAGB 1 cut(s) 257
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 5 cut(s) 350, 375, 565, 819, 1250
RsaNI GTAC 5 cut(s) 349, 374, 564, 818, 1249
RseI CAYNNNNRTG 1 cut(s) 1049
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 839, 1163
SatI GCNGC 7 cut(s) 423, 892, 996, 1112, 1171, 1185, 1188
Sau3AI GATC 5 cut(s) 66, 72, 586, 849, 1239
Sau96I GGNCC 2 cut(s) 866, 959
ScrFI CCNGG 2 cut(s) 809, 963
SduI GDGCHC 4 cut(s) 113, 623, 1029, 1209
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 866, 959
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1049
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 6 cut(s) 369, 378, 417, 636, 999, 1159
SsiI CCGC 4 cut(s) 20, 77, 422, 1173
StyD4I CCNGG 2 cut(s) 807, 961
StyI CCWWGG 4 cut(s) 114, 813, 901, 1227
TaaI ACNGT 2 cut(s) 106, 348
TaiI ACGT 2 cut(s) 530, 682
TaqI TCGA 5 cut(s) 258, 443, 607, 848, 1099
TasI AATT 6 cut(s) 369, 378, 417, 636, 999, 1159
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 4 cut(s) 34, 50, 713, 916
Tru1I TTAA 2 cut(s) 839, 1163
Tru9I TTAA 2 cut(s) 839, 1163
TscAI CASTG 3 cut(s) 166, 414, 506
TseI GCWGC 6 cut(s) 891, 995, 1111, 1170, 1184, 1187
TspDTI ATGAA 3 cut(s) 480, 672, 681
TspGWI ACGGA 2 cut(s) 909, 1000
TspRI CASTG 3 cut(s) 166, 414, 506
Van91I CCANNNNNTGG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 1205
VpaK11BI GGWCC 2 cut(s) 866, 959
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 2 cut(s) 636, 1159
XceI RCATGY 2 cut(s) 29, 465
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 982
XmnI GAANNNNTTC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.