pycom12g23140

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004626
Physical Location & Seq
Reverse (-)
23791495 .. 23808517
17023 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6255 bp
ATGGCTTCAAAATTGTCGACTGAGGATTGCATGGCATGTGTTGATCTTCTTGAAATTATGCTGGTGGAACATCTGCGGAGGGTACGAGACTGCTTTTCAGTTGGATTACTTTCACAGTTAATAATCTTTTTCATATGCCACCCATGCTGGGAAGTTCGAAGAATGACTTATGACACAACCAGAAGGATAGTTTCCGCTGCTCCGCAATTGACTGAACCTCTTTTAGTTGAATTCACGAATTTCATGTCTGTAGTTGCAGAGAAACTTCATATTTCAAAGTCAAGTGAAACAGATAATTCATTGGACACTCAAGTACCATTCCTTCCGTCGGTTGAGGTTTCAGTAAAGGCTTTAATTGTCATATCATCAGTAGCTCTCCCTGCAGCTCCAAGTGCAGTGACACGAGTTTTGTTCTGCGCTCATCATCCATATTTGGTCGGAACTGCAAAGAGAGATGCAGTTTGGAAGAGGCTACAAAAATGTATGCATGCATGTGGTTTTGACATCATTAGCAACATTTTGGCTGATGTGGATAATTTGTTCAAGGGTCTACTGGTGCCAATGTTGTTGTCCAGCACCAATCCATTTGAACAGCGAGCAGCAGTTTCGTCGTTATCCAGTTTGATGTCCATTGCACCTGGAGAAACCTATGCGGAGTTCGAAAAGCACCTGAAAAATCTTCCATATCGTTTTTCACATGACACACTTTCAGAAAATGATATTCGGATTTTTCATACCCCTGAAGGCTTGCTTTCCAGTGAGCAAGGTGTCTACATTGCGGAGTCTGTTGCTGCTAAGAACATGAAACAAGCAAAGGGTCGTTTTCGGATGTATGAGGACCTAGATGACACGGACCATGGTGGTTCTAATCATGCTGCGAAAGTTGAGCAAACCAATAGTTCTGTGGGTAAAAGGGAAACTGGGAAGTCTGCAAAGAAACCTGATAAAGGAAAGACTGCAAAGGAGGAAGCACGTGAGGTGCAACTTAGGGAGGAGGCATCCATACGCGAGAAGGTCCGAGATATACAGAAAAATTTGTCTTCCATACTTACAGCTCTTGGGGAAATGGCAATTGCTAATCCTATTTTCGCACACAGTCAACTTCCTTCCTTGGTTAACTATGTTGATCCATTGCTAAGGTCACCTATAGTCAGTGACGTGGCATATGAAACTGTGGTGAAACTCTCTAGATGCACTGCTCCACCTCTTTGTAATTGGGCCCTTGACATTGCTACAGCTTTGCGCTTAGTTGTGACTGAAGAAGTTCGTCTTGTATTGGACATGATTCCATCAGTTGGTGAAGGGGAAGCCAATGAAAGACCATCTTTGAGTCTATTTGAGCGAATAATTAATGGCCTTTCTGTATCTTGCAAAACGGGACCTCTTCCTGTAGATTCTTTTACCTTTGTGTTCCCTATAATGGAGCGAATTCTGTTATGCTCTAAGAAGACAGGACTTCACAATGATGTTCTTCAGATTCTGTATCTGCACATGGACCCCTTGTTACCTCTTCCAAGGCTTCGAATGATTTCAGTTCTTTATCATGTACTTGGTGTTGTTCCAGCTTATCAAGCCTCTGTTGGACCTGCGTTGAACGAGTTGTGTTTGGGCGTGCAACCAGATGAAGTGGCAGCAGCTCTGTACGGTGTTTATGCGAAAGATGTTCATGTTAGAATGGCCTGCCTTAGTGCTGTCAAATGTATTCCAGCTGTTACCAGTCGGTCTCTTCCTCAGAATGTTGAAGTCGCTACAGGCATTTGGATTGCCTTACATGACCCAGAAAAGTCCGTTGCAGAAGCAGCAGAGGATTTATGGGATCGGTATGGGCATGACTTTGGGACAGACTATTCAGGCCTTTTCAAAGCACTTTCGCATATCAATTATAATGTCCGTTTTGCTGCTGCTGAGGCATTAGCTGCTGCTTTGGATGAATGTCCCGATTCTATACAGGAGTCTCTTTCTACTTTATTTTCTCTGTATATCCGTGATGCTGGTTTGACTGAGGAGAATGTTGATGCTGGTTGGCTGGGTCGACAAGGTGTAGCCTTGGCATTACATTCTTCTGCTGATGTACTCAGAACAAAAGACCTTCCTGTTGTTATGACATTTCTTATTTCAAGAGCACTGGCTGATCCTAGTGCCGATGTTAGGGGAAGAATGATCAATGCTGGTATTATGATTATTGATAAGCATGGCAGGGATAATGTTTCCTTGCTATTTCCCATCTTTGAGAACTACTTGAACAAAAAGGCATCAGATGAAGAGAAATATGATTTGGTACGTGAGGGGGTTGTTATTTTCACTGGAGCGTTGGCAAAACATTTGGCAAAGGATGATCGATCAAAAGTTCATGTTGTTGTCGAGAAGTTGTTGGATGTGCTAAACACTCCATCAGAAGCTGTTCAACGGGCTGTATCTGCATGCTTGTCTCCACTAATGCAATCAAAGCAAGATGATGGACCAGCTCTTGTCTCGAGGCTTTTGGATAAACTGATGAAGAGTGACAAATATGGGGAACGCCGTGGAGCTGCTTTCGGGCTTGCTGGAGTGGTCAAGGGATTTGGTATTTCTTGCTTGAAGAAATATGGGATTGTAGCTCAACTACAAGAAGGACTTGTAGATAGGAATTCAGCAAAATGTCGTGAAGGTGCTTTACTCGGATTTGAGTGTCTCTTTCGTGAAGGTGCCGAGTGTGCGGCTCGTGCAATGATGTCTCAACTTAGTGCTCAAGGAGTTAAACTGGTTCTTCCATCTCTTTTGAAGGGTCTAGAAGATAAAGCTTGGCGAACAAAGCAAAGTAGTGTCCAACTGCTTGGGGCTATGGCCTATTGTGCTCCTCAACAGCTGTCTCAATGTCTCCCTAAGATTGTTCCAAAATTGACTGAGGTTTTAACAGATACACATCCTAAAGTCCAGTCAGCGGGACAAACGGCACTTCAACAGGTTGGGAGTGTAATAAAGAATCCTGAAATAGCTTCCCTTGTTCCTACTCTTCTACTGGGTCTTTCAGATCCAAATGACCATACAAAGTACTCTCTTGATATTCTCTTACAGACCACTTTTATTAATACAATTGATGCTCCCTCACTCGCACTGTTGGTGCCAATAGTACATAGAGGGCTACGGGAAAGGAGTGCTGAAACTAAAAAGAAAGCAGCACAAATAGTTGGCAATATGTGTTCATTAGTTACAGAACCTAAGGATATGATTCCATATATTGGTTTACTTCTTCCTGAAGTGAAAAAGGTACTTGTGGATCCAATTCCAGAAGTTCGGTCAGTTGCAGCAAGGGCCCTTGGATCCCTTATAAGGGGAATGGGCGAAGATCATTTCCCGGATCTCGTTCCATGGTTGTTTGATACACTTAAATCTGATAATAGTAATGTTGAGCGCTCTGGAGCAGCTCAAGGGTTGAGCGAGGTATTAGCTGCACTTGGAACTGAGTACTTTGAGCTTGCACTTCCTGATATTATCCGAAACTGTTCTCATCAAAAAGCATCTGTCCGTGATGGATATTTAACATTATTTAAGTATTTACCAAGGTCATTGGGCGTCCAATTCCAGAACTATTTACAGCAGGTGCTGCCTGCTATTTTAGATGGTCTTGCTGATGAAAATGAGTCTGTACGTGAGGCAGCTCTAGGTGCAGGGCATATTCTGGTTGAGCATTACGCAACAACGTCTTTACCTTTGCTCCTTCCTGTTGTTGAAGATGGTATATTTAACGATAGTTGGCGGATTCGGCAAAGCTCTGTGGAATTATTGGGTGATCTCTTATTTAAGGTTGCTGGAACTTCTGGGAAAGCTTTACTTGAGGGTGGCAGTGATGATGAAGGTGCTAGCACTGAAGCACAGGGTCGGGCTATCATTGAGGTTCTGGGAAGGGAAAAGCGGGATGAAGTGCTTGCTGCATTATATATGGTTCGAACAGATGTCAGCTTATCAGTTCGTCAGGCTGCGCTGCATGTGTGGAAAACTATTGTGGCAAATACTCCGAAGACTCTAAAGGAAATAATGCCAGTTTTAATGAATACTCTAATTGCCTCTCTTGCTTCATCGTCCTCTGAAAGGCGGCAGGTTGCTGGGCGATCACTGGGTGAGCTTGTTAGGAAACTTGGTGAAAGAGTACTTCCATGGATCATCCCAATTTTATCCCAAGGGTTAAAGGATTCTGATGTTGGCAGAAGACAAGGAGTATGCATTGGTCTGAGTGAAGTAATGGCAAGTGCTGGCAAAAATCAGTTGTTGAGTTTCATGGATGAACTCATACCGACCATTCGAACTGCTCTTTCGGATAGCATGCCTGAAGTTCGTGAGGCTGCTGGGTTAGCATTCAACACTCTCTACAAGAGTGCTGGAATGCAAGCAATAGATGAAATTGTTCCCACTCTTTTGCATGCTTTGGAGGATGATCAAACTTCTGAAACTGCTCTTGATGGCCTCAAACAAATCTTAAGTGTCAGGATTTCTGCTGTTTTGCCCCACATCTTACCCAAGCTGGTCCACCTGCCACTTACTGCGTTTAACGCACATGCTCTTGGAGCGGTAGCTGAGGTGGCTGGACCTGGTCTTAACTCTCATCTTGGTACTGTTCTTCCTGCTTTACTTTCTGCAATGAGTGCTGATGAGAAGGAAGTTCAAAAGTTGGCAAAGGAGGCTGCTGAGACTGTGGTCTTAGTCATAGATGAGGAAGGTGTTGAATCTCTTTTATCTGAGCTTGTTCGAGCTGTCAGTGATAGTCAGGCTTCAATTAGAAGAAGTTCTTCGTATCTTATTGGCTATTTCTTCAAAAGCAGTAAATTATATTTGGTTGATGAAGCTCCAAACGTGATATCTGCCCTGATTGTTTTGCTTAGTGATTCAGATTCAGCTACTGTGGCGGCTTCTTGGGAAGCTTTGTCAAGAGTTGTAAGTTCAGTTCCCAAGGAGGTACTTCCTTCTTATATTAAATTAGTACGAGATGCTGTATCTACATCCCGGGATAAGGAGCGTAGAAAGAAGAAGGGAGGACCAATTCTCATTCCTGGGTTTTGTTTACCAAAAGCACTTCAACCGTTGCTTCCAATATTTATCCAAGGTTTGACAAGTGGATCTGCTGAACTAAGAGAGCAAGCGGCTCTAGGTCTCGGTGAACTTATCGAAGTGACTAGCGAACAGGCACTGAAAGAGTTTGTCATCCCAATTACAGGGCCTCTTATCCGGATAATAGGTGATCGGTTCCCTTGGCAGGTGAAGAGTGCGATTTTGTCCACCTTGACAATCATGATAAGAAAGGGTGGAATGGCATTGAAACCTTTTCTTCCTCAGCTTCAAACAACATTTGTAAAGTGTCTACAGGATAGCACAAGAATCGTTCGTTCAAGTGCTGCCCTTGCACTCGGAAAGCTTAGTGCTCTCAGTACCCGGGTTGACCCTTTAGTTGGAGACCTCCTTTCCAGTTTGCAGGCTCCGGATAGTGGGGTTCGGGAGGCATGTTTGTCTGCTCTCGAAGGTGTCCTAAAGCATGCTGGAAAAAGTGTGAGCAGTGCTGTTAGAACTCGTCTCTACTCGCACCTGAAGGATTTGATTCATCATGATGATGACCAGGTTCGGATATCTGCTGCAAGCATTTTGGGCATCACATCCCAGTACCTAGAAGATGATCAACTGACGGAGCTGCTTCAGGAACTCTCTGATTTGCCATCATCTCTTAGCTTGTCTGCCAGGCATGGCTCCGTACTCACAATATCATCCATGCTCAGGCACAACCCTTCCACTATTTGTTTGTCTCCAGAGTTTCCGTCTATCTTGGACCGCCTTAAAGACGCCTTGATTGATGAGAAGTTCCCCCTGCGTGAAACTTCGACCAAGGCATTTGGGAGACTTCTGATTCACAAGCTCCGAAGTGATCCTTCTAATAAATCACTCCATTCGGAAATAATATCGTCCTTAGTGTCAGCTTTGCGTGATGATTCTAGTGAAGTTAGAAGAAAGGCATTATCTGCTATAAAACGAGGTTCCAAGGAAAATGCTTCAGTTATTTTGGCTCATATCAATATTATTGGACCGGCTCTTGCTGAATGCTTGAAAGATGGAAGTACTCCCGTGAGACTTGCAGCGGAAAGATGTGCTCTGCATGCTTTCCAATTGTCAAAGGGTCCTGAAAATATTCAAGCTGCACAAAAATTCATCACAGGCTTGGATGCCAGAAGAATATCGAAGCTTCCTGAAAATAGCGATGATAGTGAAGATGGTGAGGACACGGCAAGTGGCTGA

Protein Analysis

2085

Amino Acids

226.57

Weight (kDa)

6.14

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_EF3_N PF24987 893 - 978 6.8e-25 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 897 - 998 7.6e-08 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_GNC1 PF24984 1016 - 1102 9.1e-27 Elongation factor 3/GNC1 HEAT repeat
HEAT_GCN1 PF23271 1090 - 1237 1.2e-10 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1181 - 1259 9.6e-08 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1333 - 1480 7.7e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EF3_N PF24987 1342 - 1441 3e-07 EF3 N-terminal HEAT repeat
HEAT_GCN1 PF23271 1492 - 1681 5.3e-53 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C_2 PF25801 1804 - 2055 5.1e-29 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_GCN1_C PF25786 1902 - 2056 9.9e-73 GCN1-like, C-terminal HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1884, 3308
AarI CACCTGC 3 cut(s) 3568, 4515, 5218
Acc36I ACCTGC 5 cut(s) 1594, 3568, 4066, 4515, 5218
AccB1I GGYRCC 3 cut(s) 556, 2684, 3100
AccB7I CCANNNNNTGG 2 cut(s) 1295, 3218
AccBSI CCGCTC 1 cut(s) 4544
AccI GTMKAC 5 cut(s) 17, 550, 771, 2032, 5332
AccII CGCG 1 cut(s) 1008
AccIII TCCGGA 2 cut(s) 5199, 5449
AcsI RAATTY 6 cut(s) 230, 238, 1033, 1428, 2626, 6164
AcuI CTGAAG 9 cut(s) 762, 1278, 1457, 3255, 3867, 4326, 5576, 5645, 5997
AcvI CACGTG 1 cut(s) 974
AcyI GRCGYC 2 cut(s) 3552, 5805
AdeI CACNNNGTG 1 cut(s) 4097
AfeI AGCGCT 1 cut(s) 3392
AflII CTTAAG 1 cut(s) 4453
AjiI CACGTC 1 cut(s) 1159
AjnI CCWGG 5 cut(s) 637, 4564, 5023, 5583, 5702
AjuI GAANNNNNNNTTGG 2 cut(s) 2258, 2290
AloI GAACNNNNNNTCC 2 cut(s) 524, 556
Alw21I GWGCWC 5 cut(s) 2125, 2728, 2836, 5395, 6112
AlwNI CAGNNNCTG 5 cut(s) 1480, 2399, 3583, 4874, 5161
Ama87I CYCGRG 3 cut(s) 2473, 4977, 5403
Aor13HI TCCGGA 2 cut(s) 5199, 5449
Aor51HI AGCGCT 1 cut(s) 3392
AoxI GGCC 8 cut(s) 1218, 1354, 1677, 1852, 2823, 3291, 4438, 5189
ApaI GGGCCC 2 cut(s) 1222, 3295
ApoI RAATTY 6 cut(s) 230, 238, 1033, 1428, 2626, 6164
ArsI GACNNNNNNTTYG 4 cut(s) 230, 262, 391, 423
AseI ATTAAT 2 cut(s) 1350, 3066
Asp700I GAANNNNTTC 8 cut(s) 1263, 1528, 2400, 3481, 4380, 4759, 4762, 6147
AspLEI GCGC 4 cut(s) 419, 1245, 3393, 3961
AsuC2I CCSGG 5 cut(s) 3335, 4978, 4979, 5404, 5405
AsuII TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
AsuNHI GCTAGC 1 cut(s) 3839
AvaI CYCGRG 3 cut(s) 2473, 4977, 5403
AxyI CCTNAGG 1 cut(s) 3198
BaeGI GKGCMC 2 cut(s) 1222, 3295
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BamHI GGATCC 2 cut(s) 3256, 3299
BanI GGYRCC 3 cut(s) 556, 2684, 3100
BanII GRGCYC 2 cut(s) 1222, 3295
BarI GAAGNNNNNNTAC 4 cut(s) 306, 338, 2637, 2669
BauI CACGAG 2 cut(s) 402, 2700
BbrPI CACGTG 1 cut(s) 974
BbsI GAAGAC 4 cut(s) 1032, 1454, 4004, 4192
Bbv12I GWGCWC 5 cut(s) 2125, 2728, 2836, 5395, 6112
BbvCI CCTCAGC 3 cut(s) 1905, 4551, 5304
BceAI ACGGC 2 cut(s) 2505, 2946
BcgI CGANNNNNNTGC 6 cut(s) 588, 591, 622, 625, 5332, 5366
BciT130I CCWGG 5 cut(s) 639, 4566, 5025, 5585, 5704
BclI TGATCA 3 cut(s) 2160, 4411, 5641
BcnI CCSGG 5 cut(s) 3335, 4978, 4979, 5404, 5405
BfmI CTRYAG 7 cut(s) 249, 381, 1146, 1233, 1389, 1749, 5333
BfoI RGCGCY 1 cut(s) 3394
BfrI CTTAAG 1 cut(s) 4453
BfuAI ACCTGC 5 cut(s) 1594, 3568, 4066, 4515, 5218
BmcAI AGTACT 4 cut(s) 3032, 3446, 4128, 6079
BmeT110I CYCGRG 3 cut(s) 2473, 4977, 5403
BmgBI CACGTC 1 cut(s) 1159
BmrI ACTGGG 4 cut(s) 930, 3008, 4103, 5620
BmtI GCTAGC 1 cut(s) 3843
BmuI ACTGGG 4 cut(s) 930, 3008, 4103, 5620
BoxI GACNNNNGTC 1 cut(s) 4670
BpiI GAAGAC 4 cut(s) 1032, 1454, 4004, 4192
BpmI CTGGAG 5 cut(s) 660, 2325, 2565, 3417, 5754
Bpu10I CCTNAGC 5 cut(s) 1136, 1905, 4551, 5304, 5738
Bpu14I TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
BpuEI CTTGAG 4 cut(s) 294, 2712, 3390, 3833
BpuMI CCSGG 5 cut(s) 3335, 4978, 4979, 5404, 5405
Bsa29I ATCGAT 1 cut(s) 2338
BsaAI YACGTR 3 cut(s) 974, 2282, 3629
BsaBI GATNNNNATC 1 cut(s) 2901
BsaHI GRCGYC 2 cut(s) 3552, 5805
BsaI GGTCTC 3 cut(s) 1728, 5129, 5418
BsaWI WCCGGW 2 cut(s) 5199, 5449
BsaXI ACNNNNNCTCC 2 cut(s) 3678, 3708
Bse118I RCCGGY 1 cut(s) 6046
Bse21I CCTNAGG 1 cut(s) 3198
Bse3DI GCAATG 6 cut(s) 630, 774, 1130, 1227, 2712, 4620
Bse8I GATNNNNATC 1 cut(s) 2901
BseAI TCCGGA 2 cut(s) 5199, 5449
BseBI CCWGG 5 cut(s) 639, 4566, 5025, 5585, 5704
BseCI ATCGAT 1 cut(s) 2338
BseJI GATNNNNATC 1 cut(s) 2901
BseMI GCAATG 6 cut(s) 630, 774, 1130, 1227, 2712, 4620
BseRI GAGGAG 3 cut(s) 1007, 2018, 2826
BseSI GKGCMC 2 cut(s) 1222, 3295
BseYI CCCAGC 4 cut(s) 147, 2026, 4082, 4322
BsgI GTGCAG 5 cut(s) 414, 1472, 3414, 3666, 6141
Bsh1236I CGCG 1 cut(s) 1008
BshFI GGCC 8 cut(s) 1220, 1356, 1679, 1854, 2825, 3293, 4440, 5191
BshNI GGYRCC 3 cut(s) 556, 2684, 3100
BshVI ATCGAT 1 cut(s) 2338
BsiHKAI GWGCWC 5 cut(s) 2125, 2728, 2836, 5395, 6112
BsiHKCI CYCGRG 3 cut(s) 2473, 4977, 5403
BsiSI CCGG 6 cut(s) 3335, 4978, 5200, 5404, 5450, 6047
BslFI GGGAC 4 cut(s) 1392, 1852, 1920, 2937
BsmBI CGTCTC 1 cut(s) 5546
BsmFI GGGAC 4 cut(s) 1392, 1852, 1920, 2937
BsmI GAATGC 3 cut(s) 4331, 4365, 6065
BsnI GGCC 8 cut(s) 1220, 1356, 1679, 1854, 2825, 3293, 4440, 5191
Bso31I GGTCTC 3 cut(s) 1728, 5129, 5418
BsoBI CYCGRG 3 cut(s) 2473, 4977, 5403
Bsp119I TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
Bsp120I GGGCCC 2 cut(s) 1218, 3291
Bsp1286I GDGCHC 7 cut(s) 1222, 2125, 2728, 2836, 3295, 5395, 6112
Bsp13I TCCGGA 2 cut(s) 5199, 5449
Bsp19I CCATGG 3 cut(s) 856, 3347, 4133
BspANI GGCC 8 cut(s) 1220, 1356, 1679, 1854, 2825, 3293, 4440, 5191
BspDI ATCGAT 1 cut(s) 2338
BspEI TCCGGA 2 cut(s) 5199, 5449
BspFNI CGCG 1 cut(s) 1008
BspHI TCATGA 2 cut(s) 5262, 5572
BspMAI CTGCAG 1 cut(s) 385
BspMI ACCTGC 5 cut(s) 1594, 3568, 4066, 4515, 5218
BspOI GCTAGC 1 cut(s) 3843
BspT104I TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
BspT107I GGYRCC 3 cut(s) 556, 2684, 3100
BspTI CTTAAG 1 cut(s) 4453
BspTNI GGTCTC 3 cut(s) 1728, 5129, 5418
BsrBI CCGCTC 1 cut(s) 4544
BsrDI GCAATG 6 cut(s) 630, 774, 1130, 1227, 2712, 4620
BsrFI RCCGGY 1 cut(s) 6046
BssAI RCCGGY 1 cut(s) 6046
BssNI GRCGYC 2 cut(s) 3552, 5805
BssSI CACGAG 2 cut(s) 402, 2700
Bst2BI CACGAG 2 cut(s) 402, 2700
Bst2UI CCWGG 5 cut(s) 639, 4566, 5025, 5585, 5704
Bst6I CTCTTC 8 cut(s) 461, 1389, 1515, 1731, 2256, 2492, 2997, 5228
BstACI GRCGYC 2 cut(s) 3552, 5805
BstAFI CTTAAG 1 cut(s) 4453
BstAPI GCANNNNNTGC 4 cut(s) 1916, 3583, 4619, 5981
BstBAI YACGTR 3 cut(s) 974, 2282, 3629
BstBI TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
BstDSI CCRYGG 4 cut(s) 856, 2521, 3347, 4133
BstEII GGTNACC 1 cut(s) 1140
BstENI CCTNNNNNAGG 2 cut(s) 945, 4066
BstFNI CGCG 1 cut(s) 1008
BstH2I RGCGCY 1 cut(s) 3394
BstHHI GCGC 4 cut(s) 419, 1245, 3393, 3961
BstNI CCWGG 5 cut(s) 639, 4566, 5025, 5585, 5704
BstPAI GACNNNNGTC 1 cut(s) 4670
BstPI GGTNACC 1 cut(s) 1140
BstSFI CTRYAG 7 cut(s) 249, 381, 1146, 1233, 1389, 1749, 5333
BstSLI GKGCMC 2 cut(s) 1222, 3295
BstUI CGCG 1 cut(s) 1008
BstV2I GAAGAC 4 cut(s) 1032, 1454, 4004, 4192
BstX2I RGATCY 5 cut(s) 3010, 3256, 3299, 3337, 5090
BstXI CCANNNNNNTGG 1 cut(s) 2813
BstYI RGATCY 5 cut(s) 3010, 3256, 3299, 3337, 5090
Bsu15I ATCGAT 1 cut(s) 2338
Bsu36I CCTNAGG 1 cut(s) 3198
BsuRI GGCC 8 cut(s) 1220, 1356, 1679, 1854, 2825, 3293, 4440, 5191
BsuTUI ATCGAT 1 cut(s) 2338
BtgI CCRYGG 4 cut(s) 856, 2521, 3347, 4133
BtgZI GCGATG 1 cut(s) 6231
BtrI CACGTC 1 cut(s) 1159
BtsI GCAGTG 4 cut(s) 402, 1194, 3829, 5530
BveI ACCTGC 5 cut(s) 1594, 3568, 4066, 4515, 5218
CaiI CAGNNNCTG 5 cut(s) 1480, 2399, 3583, 4874, 5161
CciI TCATGA 2 cut(s) 5262, 5572
CfoI GCGC 4 cut(s) 419, 1245, 3393, 3961
Cfr10I RCCGGY 1 cut(s) 6046
Cfr9I CCCGGG 2 cut(s) 4977, 5403
ClaI ATCGAT 1 cut(s) 2338
CseI GACGC 2 cut(s) 3541, 5813
CsiI ACCWGGT 2 cut(s) 4564, 5583
DraIII CACNNNGTG 1 cut(s) 4097
Eam1104I CTCTTC 8 cut(s) 461, 1389, 1515, 1731, 2256, 2492, 2997, 5228
EarI CTCTTC 8 cut(s) 461, 1389, 1515, 1731, 2256, 2492, 2997, 5228
EciI GGCGGA 1 cut(s) 3751
Eco147I AGGCCT 1 cut(s) 1854
Eco24I GRGCYC 2 cut(s) 1222, 3295
Eco31I GGTCTC 3 cut(s) 1728, 5129, 5418
Eco32I GATATC 2 cut(s) 4833, 5595
Eco47III AGCGCT 1 cut(s) 3392
Eco57I CTGAAG 9 cut(s) 762, 1278, 1457, 3255, 3867, 4326, 5576, 5645, 5997
Eco72I CACGTG 1 cut(s) 974
Eco81I CCTNAGG 1 cut(s) 3198
Eco88I CYCGRG 3 cut(s) 2473, 4977, 5403
Eco91I GGTNACC 1 cut(s) 1140
EcoNI CCTNNNNNAGG 2 cut(s) 945, 4066
EcoO109I RGGNCCY 7 cut(s) 838, 1219, 1379, 3291, 3292, 5189, 6137
EcoO65I GGTNACC 1 cut(s) 1140
EcoRI GAATTC 3 cut(s) 230, 1428, 2626
EcoRII CCWGG 5 cut(s) 637, 4564, 5023, 5583, 5702
EcoRV GATATC 2 cut(s) 4833, 5595
EcoT22I ATGCAT 3 cut(s) 489, 493, 4202
EcoT38I GRGCYC 2 cut(s) 1222, 3295
Esp3I CGTCTC 1 cut(s) 5546
FaqI GGGAC 4 cut(s) 1392, 1852, 1920, 2937
FauI CCCGC 2 cut(s) 2914, 3885
FauNDI CATATG 2 cut(s) 134, 1165
FbaI TGATCA 3 cut(s) 2160, 4411, 5641
FblI GTMKAC 5 cut(s) 17, 550, 771, 2032, 5332
FriOI GRGCYC 2 cut(s) 1222, 3295
GlaI GCGC 4 cut(s) 418, 1244, 3392, 3960
GsaI CCCAGC 4 cut(s) 151, 2030, 4086, 4326
GsuI CTGGAG 5 cut(s) 660, 2325, 2565, 3417, 5754
HaeII RGCGCY 1 cut(s) 3394
HaeIII GGCC 8 cut(s) 1220, 1356, 1679, 1854, 2825, 3293, 4440, 5191
HapII CCGG 6 cut(s) 3335, 4978, 5200, 5404, 5450, 6047
HgaI GACGC 2 cut(s) 3541, 5813
HhaI GCGC 4 cut(s) 419, 1245, 3393, 3961
Hin1I GRCGYC 2 cut(s) 3552, 5805
Hin6I GCGC 4 cut(s) 417, 1243, 3391, 3959
HinP1I GCGC 4 cut(s) 417, 1243, 3391, 3959
HincII GTYRAC 5 cut(s) 18, 1100, 1117, 2033, 5410
HindII GTYRAC 5 cut(s) 18, 1100, 1117, 2033, 5410
HindIII AAGCTT 5 cut(s) 2778, 3804, 4893, 5384, 6200
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 6 cut(s) 3335, 4978, 5200, 5404, 5450, 6047
Hpy99I CGWCG 2 cut(s) 331, 613
HpyCH4IV ACGT 6 cut(s) 973, 1158, 2281, 3628, 3680, 4827
HpySE526I ACGT 6 cut(s) 973, 1158, 2281, 3628, 3680, 4827
Hsp92I GRCGYC 2 cut(s) 3552, 5805
HspAI GCGC 4 cut(s) 417, 1243, 3391, 3959
Kpn2I TCCGGA 2 cut(s) 5199, 5449
Ksp22I TGATCA 3 cut(s) 2160, 4411, 5641
KspAI GTTAAC 1 cut(s) 1117
MabI ACCWGGT 2 cut(s) 4564, 5583
MaeII ACGT 6 cut(s) 973, 1158, 2281, 3628, 3680, 4827
MaeIII GTNAC 9 cut(s) 397, 1140, 1154, 1252, 1503, 1711, 2501, 3187, 5143
MbiI CCGCTC 1 cut(s) 4544
MfeI CAATTG 4 cut(s) 206, 1071, 3072, 6125
MflI RGATCY 5 cut(s) 3010, 3256, 3299, 3337, 5090
MhlI GDGCHC 7 cut(s) 1222, 2125, 2728, 2836, 3295, 5395, 6112
MlyI GAGTC 5 cut(s) 791, 1339, 1961, 3629, 3994
MmeI TCCRAC 6 cut(s) 82, 418, 1561, 2352, 2830, 5401
Mph1103I ATGCAT 3 cut(s) 489, 493, 4202
MroI TCCGGA 2 cut(s) 5199, 5449
MroXI GAANNNNTTC 8 cut(s) 1263, 1528, 2400, 3481, 4380, 4759, 4762, 6147
MslI CAYNNNNRTG 4 cut(s) 492, 1464, 5574, 5577
MspA1I CMGCKG 5 cut(s) 197, 1709, 2845, 2921, 6098
MspCI CTTAAG 1 cut(s) 4453
MspI CCGG 6 cut(s) 3335, 4978, 5200, 5404, 5450, 6047
MunI CAATTG 4 cut(s) 206, 1071, 3072, 6125
Mva1269I GAATGC 3 cut(s) 4331, 4365, 6065
MvaI CCWGG 5 cut(s) 639, 4566, 5025, 5585, 5704
MvnI CGCG 1 cut(s) 1008
NciI CCSGG 5 cut(s) 3335, 4978, 4979, 5404, 5405
NcoI CCATGG 3 cut(s) 856, 3347, 4133
NdeI CATATG 2 cut(s) 134, 1165
NheI GCTAGC 1 cut(s) 3839
NmeAIII GCCGAG 1 cut(s) 2713
NmuCI GTSAC 6 cut(s) 397, 1140, 1154, 1252, 2501, 5143
NsiI ATGCAT 3 cut(s) 489, 493, 4202
NspV TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
PaeI GCATGC 6 cut(s) 491, 2424, 4303, 4400, 5507, 6119
PaeR7I CTCGAG 1 cut(s) 2473
PagI TCATGA 2 cut(s) 5262, 5572
PaqCI CACCTGC 3 cut(s) 3568, 4515, 5218
PceI AGGCCT 1 cut(s) 1854
PctI GAATGC 3 cut(s) 4331, 4365, 6065
PdmI GAANNNNTTC 8 cut(s) 1263, 1528, 2400, 3481, 4380, 4759, 4762, 6147
PflFI GACNNNGTC 1 cut(s) 4566
PflMI CCANNNNNTGG 2 cut(s) 1295, 3218
PfoI TCCNGGA 1 cut(s) 3333
PleI GAGTC 5 cut(s) 790, 1338, 1960, 3628, 3994
PmaCI CACGTG 1 cut(s) 974
PmlI CACGTG 1 cut(s) 974
PpsI GAGTC 5 cut(s) 790, 1338, 1960, 3628, 3994
Ppu21I YACGTR 3 cut(s) 974, 2282, 3629
PpuMI RGGWCCY 3 cut(s) 838, 1379, 6137
PshAI GACNNNNGTC 1 cut(s) 4670
PshBI ATTAAT 2 cut(s) 1350, 3066
PsiI TTATAA 2 cut(s) 1884, 3308
Psp5II RGGWCCY 3 cut(s) 838, 1379, 6137
Psp6I CCWGG 5 cut(s) 637, 4564, 5023, 5583, 5702
PspCI CACGTG 1 cut(s) 974
PspEI GGTNACC 1 cut(s) 1140
PspFI CCCAGC 4 cut(s) 147, 2026, 4082, 4322
PspGI CCWGG 5 cut(s) 637, 4564, 5023, 5583, 5702
PspOMI GGGCCC 2 cut(s) 1218, 3291
PspPPI RGGWCCY 3 cut(s) 838, 1379, 6137
PsrI GAACNNNNNNTAC 2 cut(s) 4902, 4934
PstI CTGCAG 1 cut(s) 385
PstNI CAGNNNCTG 5 cut(s) 1480, 2399, 3583, 4874, 5161
PsuI RGATCY 5 cut(s) 3010, 3256, 3299, 3337, 5090
PsyI GACNNNGTC 1 cut(s) 4566
PvuII CAGCTG 2 cut(s) 1709, 2845
RseI CAYNNNNRTG 4 cut(s) 492, 1464, 5574, 5577
SalI GTCGAC 2 cut(s) 16, 2031
ScaI AGTACT 4 cut(s) 3032, 3446, 4128, 6079
SchI GAGTC 5 cut(s) 791, 1339, 1961, 3629, 3994
SduI GDGCHC 7 cut(s) 1222, 2125, 2728, 2836, 3295, 5395, 6112
SexAI ACCWGGT 2 cut(s) 4564, 5583
SfcI CTRYAG 7 cut(s) 249, 381, 1146, 1233, 1389, 1749, 5333
Sfr274I CTCGAG 1 cut(s) 2473
SfuI TTCGAA 5 cut(s) 157, 660, 1522, 3925, 4279
SlaI CTCGAG 1 cut(s) 2473
SmaI CCCGGG 2 cut(s) 4979, 5405
SmiMI CAYNNNNRTG 4 cut(s) 492, 1464, 5574, 5577
SmlI CTYRAG 6 cut(s) 309, 2473, 2727, 3405, 3812, 4453
SmoI CTYRAG 6 cut(s) 309, 2473, 2727, 3405, 3812, 4453
SphI GCATGC 6 cut(s) 491, 2424, 4303, 4400, 5507, 6119
SseBI AGGCCT 1 cut(s) 1854
SspI AATATT 3 cut(s) 5067, 6037, 6148
StuI AGGCCT 1 cut(s) 1854
TaiI ACGT 6 cut(s) 976, 1161, 2284, 3631, 3683, 4830
TaqII GACCGA 2 cut(s) 1710, 3264
TatI WGTACW 7 cut(s) 1546, 2071, 3030, 3109, 3444, 4126, 6077
TauI GCSGC 4 cut(s) 2699, 4075, 4883, 5117
TseFI GTSAC 6 cut(s) 397, 1140, 1154, 1252, 2501, 5143
Tsp45I GTSAC 6 cut(s) 397, 1140, 1154, 1252, 2501, 5143
TspGWI ACGGA 9 cut(s) 315, 866, 1777, 1880, 1973, 3494, 5666, 5704, 5769
TspMI CCCGGG 2 cut(s) 4977, 5403
Tth111I GACNNNGTC 1 cut(s) 4566
Van91I CCANNNNNTGG 2 cut(s) 1295, 3218
Vha464I CTTAAG 1 cut(s) 4453
VspI ATTAAT 2 cut(s) 1350, 3066
XagI CCTNNNNNAGG 2 cut(s) 945, 4066
XapI RAATTY 6 cut(s) 230, 238, 1033, 1428, 2626, 6164
XbaI TCTAGA 2 cut(s) 1187, 2767
XcmI CCANNNNNNNNNTGG 1 cut(s) 901
XhoI CTCGAG 1 cut(s) 2473
XmaI CCCGGG 2 cut(s) 4977, 5403
XmiI GTMKAC 5 cut(s) 17, 550, 771, 2032, 5332
XmnI GAANNNNTTC 8 cut(s) 1263, 1528, 2400, 3481, 4380, 4759, 4762, 6147
ZrmI AGTACT 4 cut(s) 3032, 3446, 4128, 6079
Zsp2I ATGCAT 3 cut(s) 489, 493, 4202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.