pycom13g00750

Protein of unknown function (DUF3755)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004772
Physical Location & Seq
Reverse (-)
467719 .. 470252
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGGCGATGGAGTCGAACACAGGATATCTCCGCGACGAAGCTTTTGGCTACGGTTTGAACCGACACGCTATATCGTTTCAGTCCGGGGCCATACACAGCAGCTCGGAGATGATTCCGATGGGGAATTACTTTGCTACGGACGGGGGCATGATGTTTTCGTCGATTTCAGGCATAGTTAACAACAATCCTGTAATTAGTCAGGCTGGGAATTCATCAGGTTCTCTGCTTCTCGATTCGGTCCCCGGGCTCAAGCATGACACAGGCTTGGCTGTCGAGTGGTCTGTGGAGGAACAGTACAACTTGACGGAGGGCCTTATCAAATATGCTGATGAACCAAGTATTATGAAATACATAAAGATCGCGGCAATGATACGTGATAAAACTGTGCGTGATGTTGCTTTGAGGTGTAGGTGGATGACTAGAAAGCGTAGGAAACCTGAAGAACATAGTATGGGAAAGAAGGGGAACATTAGGAAGGATAAACTCGTGGAGTCGTCTTCAAAGACAAACATACATTCAGCTGCACCACTGGACATGGCTGCATATTCTCATTCGGTGCACCATATGGACCAGAATGAGCGTTTGCAGTATGAAGGATTAAGTGGCACATTCAAGCATCTATTGGAGCAAAATGCTCAAGCTTTCAGTCAAATTACATCTAATATTTCGTCATACAAGTTGCAGGAAAACATTGACCTCTTCTACCGCACGAGGAACAATTTAAATGCCATCCTTAACAACATGAGAGGTATGCCTGGCATAATGAGCCGAATGCCGCCACTGCCACCCATTGACGAGCGATCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3755

Protein Analysis

270

Amino Acids

30.13

Weight (kDa)

8.44

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3755 PF12579 224 - 257 5.8e-18 Protein of unknown function (DUF3755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 33, 362
AciI CCGC 4 cut(s) 31, 362, 706, 776
AcsI RAATTY 1 cut(s) 208
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 1 cut(s) 296
AgsI TTSAA 3 cut(s) 58, 501, 613
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 4 cut(s) 41, 102, 521, 641
AluI AGCT 4 cut(s) 41, 102, 521, 641
Alw21I GWGCWC 1 cut(s) 561
Alw44I GTGCAC 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 2 cut(s) 87, 310
ApaLI GTGCAC 1 cut(s) 557
ApeKI GCWGC 3 cut(s) 99, 521, 539
ApoI RAATTY 1 cut(s) 208
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 4 cut(s) 87, 238, 310, 568
AsuC2I CCSGG 3 cut(s) 85, 243, 244
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 238, 568
BaeGI GKGCMC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 2 cut(s) 485, 709
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 111, 508, 526
BccI CCATC 2 cut(s) 112, 737
BciT130I CCWGG 1 cut(s) 756
BcnI CCSGG 3 cut(s) 85, 243, 244
BfaI CTAG 1 cut(s) 420
BisI GCNGC 5 cut(s) 100, 363, 522, 540, 776
BlsI GCNGC 5 cut(s) 101, 364, 523, 541, 777
Bme1390I CCNGG 4 cut(s) 85, 243, 244, 756
Bme18I GGWCC 2 cut(s) 238, 568
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 4 cut(s) 87, 238, 310, 568
BmiI GGNNCC 2 cut(s) 88, 240
BmrFI CCNGG 4 cut(s) 85, 243, 244, 756
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 233, 621
BpuMI CCSGG 3 cut(s) 85, 243, 244
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 84, 241, 242
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 84, 241, 242
BseGI GGATG 2 cut(s) 420, 729
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 534
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 3 cut(s) 111, 508, 526
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 33, 362
BshFI GGCC 2 cut(s) 89, 312
BsiHKAI GWGCWC 1 cut(s) 561
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 84, 243
BslFI GGGAC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 2 cut(s) 89, 312
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 249, 561
Bsp143I GATC 2 cut(s) 357, 800
BspACI CCGC 4 cut(s) 31, 362, 706, 776
BspANI GGCC 2 cut(s) 89, 312
BspFNI CGCG 2 cut(s) 33, 362
BspLI GGNNCC 2 cut(s) 88, 240
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 534
BssECI CCNNGG 3 cut(s) 84, 241, 242
BssMI GATC 2 cut(s) 357, 800
BssSI CACGAG 2 cut(s) 485, 709
Bst2BI CACGAG 2 cut(s) 485, 709
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 3 cut(s) 53, 294, 385
Bst6I CTCTTC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 374
BstF5I GGATG 2 cut(s) 420, 729
BstFNI CGCG 2 cut(s) 33, 362
BstKTI GATC 2 cut(s) 360, 803
BstMBI GATC 2 cut(s) 357, 800
BstMWI GCNNNNNNNGC 2 cut(s) 765, 781
BstNI CCWGG 1 cut(s) 756
BstSCI CCNGG 4 cut(s) 83, 241, 242, 754
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 2 cut(s) 33, 362
BstV1I GCAGC 3 cut(s) 111, 508, 526
BstV2I GAAGAC 1 cut(s) 489
BsuRI GGCC 2 cut(s) 89, 312
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 420, 729
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 2 cut(s) 527, 779
Cfr13I GGNCC 4 cut(s) 87, 238, 310, 568
Cfr9I CCCGGG 1 cut(s) 242
Csp6I GTAC 1 cut(s) 295
CviAII CATG 4 cut(s) 148, 254, 535, 742
CviQI GTAC 1 cut(s) 295
DpnI GATC 2 cut(s) 359, 802
DpnII GATC 2 cut(s) 357, 800
DraI TTTAAA 1 cut(s) 723
Eam1104I CTCTTC 1 cut(s) 704
EarI CTCTTC 1 cut(s) 704
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 238, 568
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 4 cut(s) 151, 257, 538, 745
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 147, 253, 534, 741
FauNDI CATATG 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 100, 363, 522, 540, 776
FokI GGATG 2 cut(s) 427, 716
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 100, 363, 522, 540, 776
FspBI CTAG 1 cut(s) 420
GluI GCNGC 5 cut(s) 100, 363, 522, 540, 776
GsaI CCCAGC 1 cut(s) 207
HaeIII GGCC 2 cut(s) 89, 312
HapII CCGG 2 cut(s) 84, 243
Hin1II CATG 4 cut(s) 151, 257, 538, 745
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 2 cut(s) 39, 639
HinfI GANTC 4 cut(s) 11, 112, 233, 491
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 2 cut(s) 84, 243
Hpy166II GTNNAC 2 cut(s) 178, 559
Hpy188I TCNGA 2 cut(s) 106, 117
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 2 cut(s) 178, 559
Hpy99I CGWCG 2 cut(s) 38, 163
HpyAV CCTTC 3 cut(s) 454, 469, 587
HpyCH4III ACNGT 3 cut(s) 53, 294, 385
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 5 cut(s) 524, 542, 559, 586, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 765, 781
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 4 cut(s) 151, 257, 538, 745
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 357, 800
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 3 cut(s) 111, 508, 526
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 373
MalI GATC 2 cut(s) 359, 802
MboI GATC 2 cut(s) 357, 800
MboII GAAGA 3 cut(s) 452, 489, 691
MhlI GDGCHC 2 cut(s) 249, 561
MluCI AATT 5 cut(s) 124, 192, 208, 651, 718
MlyI GAGTC 2 cut(s) 20, 500
MnlI CCTC 6 cut(s) 280, 301, 396, 705, 707, 740
MseI TTAA 4 cut(s) 177, 599, 722, 735
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 84, 243
MspR9I CCNGG 4 cut(s) 85, 243, 244, 756
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 2 cut(s) 33, 362
MwoI GCNNNNNNNGC 2 cut(s) 765, 781
NciI CCSGG 3 cut(s) 85, 243, 244
NdeI CATATG 1 cut(s) 564
NdeII GATC 2 cut(s) 357, 800
NlaIII CATG 4 cut(s) 151, 257, 538, 745
NlaIV GGNNCC 2 cut(s) 88, 240
PctI GAATGC 1 cut(s) 777
PfeI GAWTC 2 cut(s) 112, 233
PkrI GCNGC 5 cut(s) 101, 364, 523, 541, 777
PleI GAGTC 2 cut(s) 19, 499
PpsI GAGTC 2 cut(s) 19, 499
Ppu21I YACGTR 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 88, 240
PspPI GGNCC 4 cut(s) 87, 238, 310, 568
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SaqAI TTAA 4 cut(s) 177, 599, 722, 735
SatI GCNGC 5 cut(s) 100, 363, 522, 540, 776
Sau3AI GATC 2 cut(s) 357, 800
Sau96I GGNCC 4 cut(s) 87, 238, 310, 568
SchI GAGTC 2 cut(s) 20, 500
ScrFI CCNGG 4 cut(s) 85, 243, 244, 756
SduI GDGCHC 2 cut(s) 249, 561
SfaNI GCATC 1 cut(s) 625
SinI GGWCC 2 cut(s) 238, 568
SmaI CCCGGG 1 cut(s) 244
SmiI ATTTAAAT 1 cut(s) 723
SmlI CTYRAG 2 cut(s) 248, 636
SmoI CTYRAG 2 cut(s) 248, 636
Sse9I AATT 5 cut(s) 124, 192, 208, 651, 718
SsiI CCGC 4 cut(s) 31, 362, 706, 776
SspI AATATT 1 cut(s) 664
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 4 cut(s) 83, 241, 242, 754
SwaI ATTTAAAT 1 cut(s) 723
TaaI ACNGT 3 cut(s) 53, 294, 385
TaiI ACGT 1 cut(s) 376
TaqI TCGA 4 cut(s) 14, 161, 231, 273
TaqII GACCGA 1 cut(s) 226
TasI AATT 5 cut(s) 124, 192, 208, 651, 718
TatI WGTACW 1 cut(s) 294
TauI GCSGC 2 cut(s) 365, 778
TfiI GAWTC 2 cut(s) 112, 233
Tru1I TTAA 4 cut(s) 177, 599, 722, 735
Tru9I TTAA 4 cut(s) 177, 599, 722, 735
TscAI CASTG 2 cut(s) 534, 786
TseI GCWGC 3 cut(s) 99, 521, 539
TspDTI ATGAA 4 cut(s) 201, 345, 359, 606
TspGWI ACGGA 2 cut(s) 152, 320
TspMI CCCGGG 1 cut(s) 242
TspRI CASTG 2 cut(s) 534, 786
VneI GTGCAC 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 238, 568
XapI RAATTY 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 242
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.