pycom13g00920

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006076
Physical Location & Seq
Reverse (-)
591540 .. 592691
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGTCTCTCCCTTTCTTTTCCGGCCATGGAAATGTCGTGGCTGTTGCACTCGTGATGTTGGTTTTCATGAGCGCAACAAGTGAAGCGCAATTATCTTCTACATTTTATGACAAAACCTGTCCAAACGCTCTTACCACTATCAAAAATGCCGTAAGAACAGCTGTTTCAAGTGAAAGGCGGATGGCCGCGTCCCTAATCCGCCTCCATTTCCATGATTGCTTTGTCCAGGGTTGTGATGCATCAATCTTACTTGATGACGCTCCTTCTATCACAAGTGAAAAGGACGCATTGCAAAACAACGGTTCTGTGAGAGGCTTTGAAGTCATAGACAAAGCAAAAGCTGAAGTGGAGAAGGTCTGCCCTGGTGTCGTATCTTGCGCAGATATTCTCGCTGTCGCCGCTCGGGATGCATCCGTTGCCGTGAGCGGTCCATCTTGGACGGTGAAACTTGGAAGAAGAGATTCCACCACGGCAAGCCAAAGCCTAGCTGAAAGTTCACTTCCACTTTTCACGGACAGCCTTGACCGCCTTAAAGAGCGATTTGGCGACTTAGGCCTCAACGCGAGAGATTTGGTTGCACTGTCAGGTGCACATACAATTGGACAGGCTCGTTGCGTTACATTCCGTGGCAGGATTTACAACAATGCAAGTGACATCGATTCTAACTTTGCCACTACTCGCAAACGTCGTTGTCCAGCTGACGCAAACAACGGCGATGCAAATTTGGCACCGCTCGAGTTGGTGACACCGAACTCATTTGACAACAATTACTACAAGAATTTGATTCAGAAGAAGGGTCTTCTTGAATCGGATCAAGTTCTTTTTAGTGGAGGTTCCACAGACAGCATCGTCTCCGAGTACAGTTCCAAGCCCGCAACTTTTAAGGCTGATTTTATCACCGCTATGATTAAAATGGGAGACATTAATCCTCTCACCGGTTCAGCTGGAGAGGTCAGAAGGATTTGCAGTGCTCTCAACTAA

Protein Analysis

327

Amino Acids

34.89

Weight (kDa)

6.44

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 289 1.3e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 848
Acc16I TGCGCA 1 cut(s) 379
AccB1I GGYRCC 1 cut(s) 727
AccBSI CCGCTC 3 cut(s) 401, 426, 733
AccII CGCG 2 cut(s) 188, 563
AciI CCGC 9 cut(s) 178, 186, 199, 399, 426, 526, 731, 873, 900
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 2 cut(s) 22, 183
AcsI RAATTY 2 cut(s) 721, 778
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 1 cut(s) 935
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 3 cut(s) 168, 320, 806
AjnI CCWGG 2 cut(s) 225, 361
AluBI AGCT 5 cut(s) 161, 341, 488, 698, 944
AluI AGCT 5 cut(s) 161, 341, 488, 698, 944
Alw21I GWGCWC 2 cut(s) 592, 973
Alw26I GTCTC 3 cut(s) 9, 856, 912
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 1 cut(s) 819
Ama87I CYCGRG 2 cut(s) 402, 734
AoxI GGCC 3 cut(s) 22, 183, 553
ApaLI GTGCAC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 721, 778
AseI ATTAAT 1 cut(s) 924
AsiGI ACCGGT 1 cut(s) 935
Asp700I GAANNNNTTC 1 cut(s) 460
AspLEI GCGC 3 cut(s) 74, 88, 380
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 4 cut(s) 454, 754, 889, 925
AvaI CYCGRG 2 cut(s) 402, 734
AvaII GGWCC 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 727
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 791
Bbv12I GWGCWC 2 cut(s) 592, 973
BccI CCATC 2 cut(s) 175, 439
BceAI ACGGC 4 cut(s) 134, 404, 486, 727
BciT130I CCWGG 2 cut(s) 227, 363
BcoDI GTCTC 3 cut(s) 9, 856, 912
BfaI CTAG 1 cut(s) 485
BisI GCNGC 2 cut(s) 186, 399
BlsI GCNGC 2 cut(s) 187, 400
Bme1390I CCNGG 2 cut(s) 227, 363
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 402, 734
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 2 cut(s) 729, 835
BmrFI CCNGG 2 cut(s) 227, 363
BmsI GCATC 6 cut(s) 226, 248, 397, 419, 706, 855
BpiI GAAGAC 1 cut(s) 791
BpmI CTGGAG 1 cut(s) 966
Bsa29I ATCGAT 1 cut(s) 657
BsaJI CCNNGG 5 cut(s) 25, 226, 361, 468, 625
BsaWI WCCGGW 1 cut(s) 935
Bsc4I CCNNNNNNNGG 1 cut(s) 935
Bse118I RCCGGY 1 cut(s) 935
Bse3DI GCAATG 1 cut(s) 287
BseBI CCWGG 2 cut(s) 227, 363
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 5 cut(s) 25, 226, 361, 468, 625
BseGI GGATG 3 cut(s) 186, 410, 412
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMI GCAATG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 592
Bsh1236I CGCG 2 cut(s) 188, 563
BshFI GGCC 3 cut(s) 24, 185, 555
BshNI GGYRCC 1 cut(s) 727
BshTI ACCGGT 1 cut(s) 935
BshVI ATCGAT 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 592, 973
BsiHKCI CYCGRG 2 cut(s) 402, 734
BsiSI CCGG 2 cut(s) 21, 936
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 3 cut(s) 9, 856, 912
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 3 cut(s) 24, 185, 555
BsoBI CYCGRG 2 cut(s) 402, 734
Bsp1286I GDGCHC 2 cut(s) 592, 973
Bsp143I GATC 1 cut(s) 811
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 9 cut(s) 178, 186, 199, 399, 426, 526, 731, 873, 900
BspANI GGCC 3 cut(s) 24, 185, 555
BspDI ATCGAT 1 cut(s) 657
BspFNI CGCG 2 cut(s) 188, 563
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 729, 835
BspPI GGATC 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 727
BsrBI CCGCTC 3 cut(s) 401, 426, 733
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 935
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 5 cut(s) 25, 226, 361, 468, 625
BssMI GATC 1 cut(s) 811
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 227, 363
Bst4CI ACNGT 4 cut(s) 302, 442, 582, 863
Bst6I CTCTTC 1 cut(s) 451
BstAPI GCANNNNNTGC 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 475, 873
BstDEI CTNAG 1 cut(s) 550
BstDSI CCRYGG 3 cut(s) 25, 468, 625
BstF5I GGATG 3 cut(s) 186, 410, 412
BstFNI CGCG 2 cut(s) 188, 563
BstHHI GCGC 3 cut(s) 74, 88, 380
BstKTI GATC 1 cut(s) 814
BstMAI GTCTC 3 cut(s) 9, 856, 912
BstMBI GATC 1 cut(s) 811
BstMWI GCNNNNNNNGC 6 cut(s) 398, 407, 416, 525, 552, 725
BstNI CCWGG 2 cut(s) 227, 363
BstSCI CCNGG 2 cut(s) 225, 361
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 2 cut(s) 188, 563
BstV2I GAAGAC 1 cut(s) 791
Bsu15I ATCGAT 1 cut(s) 657
BsuRI GGCC 3 cut(s) 24, 185, 555
BsuTUI ATCGAT 1 cut(s) 657
BtgI CCRYGG 3 cut(s) 25, 468, 625
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 3 cut(s) 186, 410, 412
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 2 cut(s) 578, 973
Cac8I GCNNGC 2 cut(s) 475, 873
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 74, 88, 380
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 1 cut(s) 428
ClaI ATCGAT 1 cut(s) 657
CseI GACGC 4 cut(s) 177, 266, 293, 710
Csp6I GTAC 1 cut(s) 859
CspAI ACCGGT 1 cut(s) 935
CviAII CATG 3 cut(s) 26, 67, 212
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 1 cut(s) 550
DpnI GATC 1 cut(s) 813
DpnII GATC 1 cut(s) 811
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
EaeI YGGCCR 2 cut(s) 22, 183
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
EciI GGCGGA 2 cut(s) 188, 193
Eco130I CCWWGG 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 363
Eco88I CYCGRG 2 cut(s) 402, 734
EcoRII CCWGG 2 cut(s) 225, 361
EcoT14I CCWWGG 1 cut(s) 25
EcoT22I ATGCAT 2 cut(s) 241, 412
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 3 cut(s) 29, 70, 215
FaiI YATR 7 cut(s) 27, 68, 108, 213, 326, 594, 905
FaqI GGGAC 1 cut(s) 175
FatI CATG 3 cut(s) 25, 66, 211
FauI CCCGC 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 186, 399
FokI GGATG 3 cut(s) 193, 397, 419
Fsp4HI GCNGC 2 cut(s) 186, 399
FspBI CTAG 1 cut(s) 485
FspI TGCGCA 1 cut(s) 379
GlaI GCGC 3 cut(s) 73, 87, 379
GluI GCNGC 2 cut(s) 186, 399
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 3 cut(s) 24, 185, 555
HapII CCGG 2 cut(s) 21, 936
HgaI GACGC 4 cut(s) 177, 266, 293, 710
HhaI GCGC 3 cut(s) 74, 88, 380
Hin1II CATG 3 cut(s) 29, 70, 215
Hin6I GCGC 3 cut(s) 72, 86, 378
HinP1I GCGC 3 cut(s) 72, 86, 378
HinfI GANTC 4 cut(s) 461, 659, 784, 806
HpaII CCGG 2 cut(s) 21, 936
HphI GGTGA 4 cut(s) 454, 754, 889, 925
Hpy166II GTNNAC 2 cut(s) 497, 590
Hpy188I TCNGA 4 cut(s) 789, 811, 856, 956
Hpy188III TCNNGA 4 cut(s) 52, 67, 404, 803
Hpy8I GTNNAC 2 cut(s) 497, 590
Hpy99I CGWCG 1 cut(s) 690
HpyAV CCTTC 4 cut(s) 273, 346, 787, 951
HpyCH4III ACNGT 4 cut(s) 302, 442, 582, 863
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 9 cut(s) 47, 239, 292, 410, 578, 590, 647, 719, 966
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 407, 416, 525, 552, 725
HpyF3I CTNAG 1 cut(s) 550
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 3 cut(s) 29, 70, 215
HspAI GCGC 3 cut(s) 72, 86, 378
Kzo9I GATC 1 cut(s) 811
LmnI GCTCC 1 cut(s) 265
LweI GCATC 6 cut(s) 226, 248, 397, 419, 706, 855
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 3 cut(s) 616, 650, 742
MalI GATC 1 cut(s) 813
MbiI CCGCTC 3 cut(s) 401, 426, 733
MboI GATC 1 cut(s) 811
MboII GAAGA 5 cut(s) 87, 465, 468, 791, 802
MfeI CAATTG 1 cut(s) 597
MhlI GDGCHC 2 cut(s) 592, 973
MluCI AATT 5 cut(s) 89, 597, 721, 766, 778
MnlI CCTC 6 cut(s) 212, 305, 566, 824, 939, 943
Mph1103I ATGCAT 2 cut(s) 241, 412
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 4 cut(s) 531, 882, 909, 924
MslI CAYNNNNRTG 3 cut(s) 30, 210, 902
MspA1I CMGCKG 3 cut(s) 161, 698, 944
MspI CCGG 2 cut(s) 21, 936
MspR9I CCNGG 2 cut(s) 227, 363
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 2 cut(s) 227, 363
MvnI CGCG 2 cut(s) 188, 563
MwoI GCNNNNNNNGC 6 cut(s) 398, 407, 416, 525, 552, 725
NcoI CCATGG 1 cut(s) 25
NdeII GATC 1 cut(s) 811
NlaIII CATG 3 cut(s) 29, 70, 215
NlaIV GGNNCC 2 cut(s) 729, 835
NmuCI GTSAC 2 cut(s) 650, 742
NsbI TGCGCA 1 cut(s) 379
NsiI ATGCAT 2 cut(s) 241, 412
PaeR7I CTCGAG 1 cut(s) 734
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 4 cut(s) 461, 659, 784, 806
PinAI ACCGGT 1 cut(s) 935
PkrI GCNGC 2 cut(s) 187, 400
PshBI ATTAAT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 225, 361
PspGI CCWGG 2 cut(s) 225, 361
PspN4I GGNNCC 2 cut(s) 729, 835
PspPI GGNCC 1 cut(s) 428
PspXI VCTCGAGB 1 cut(s) 734
PvuII CAGCTG 3 cut(s) 161, 698, 944
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
RseI CAYNNNNRTG 3 cut(s) 30, 210, 902
SaqAI TTAA 4 cut(s) 531, 882, 909, 924
SatI GCNGC 2 cut(s) 186, 399
Sau3AI GATC 1 cut(s) 811
Sau96I GGNCC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 227, 363
SduI GDGCHC 2 cut(s) 592, 973
SfaNI GCATC 6 cut(s) 226, 248, 397, 419, 706, 855
Sfr274I CTCGAG 1 cut(s) 734
SinI GGWCC 1 cut(s) 428
SlaI CTCGAG 1 cut(s) 734
SmiMI CAYNNNNRTG 3 cut(s) 30, 210, 902
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 5 cut(s) 89, 597, 721, 766, 778
SseBI AGGCCT 1 cut(s) 555
SsiI CCGC 9 cut(s) 178, 186, 199, 399, 426, 526, 731, 873, 900
SspMI CTAG 1 cut(s) 485
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 2 cut(s) 225, 361
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 4 cut(s) 302, 442, 582, 863
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 657, 735
TasI AATT 5 cut(s) 89, 597, 721, 766, 778
TatI WGTACW 1 cut(s) 858
TauI GCSGC 2 cut(s) 188, 401
TfiI GAWTC 4 cut(s) 461, 659, 784, 806
Tru1I TTAA 4 cut(s) 531, 882, 909, 924
Tru9I TTAA 4 cut(s) 531, 882, 909, 924
TscAI CASTG 2 cut(s) 585, 973
TseFI GTSAC 2 cut(s) 650, 742
Tsp45I GTSAC 2 cut(s) 650, 742
TspDTI ATGAA 1 cut(s) 55
TspGWI ACGGA 3 cut(s) 403, 527, 614
TspRI CASTG 2 cut(s) 585, 973
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 428
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 2 cut(s) 721, 778
XhoI CTCGAG 1 cut(s) 734
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 1 cut(s) 485
Zsp2I ATGCAT 2 cut(s) 241, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.