pycom13g01140

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003764
Physical Location & Seq
Reverse (-)
745766 .. 748539
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGCGGGCGTTCTTTTGTGAACTGTTGGCCTTCAACGGGATTTCTACCAATCTTGTCAACTATTTCACCGATAAACTGCACGAAGAAACTGTTGTTGCTTCAACAAGTATCACTAGTTGGAAAGGAAATGCTATATCGCACCCGGGCTGTTCGTGTCCATTCCATGCATGGCAGCTGCTCTTGGGCCTTAAATTTCTACACCGACCCACGCCCCGTTTAACCGAACCCGATTGGCTTAAACCTAAAACATTCTTCCACAATCTGCCTCTCTCTTACGTTCGCCGTCGCCACTCCCTGGGAATCCTGATTCTGCACCAGCAAAATCTCCGTCGATTCCTTACTTCTCCGACTCCGACGATGAGGCCGATACTGATGAAAGGGCACGAGAGGCCCTTGACTTTCCTCAAATACAACAGGGACGGTGACCTCCTCTTCTCCTGCGCCAAGGACCACAACCCCACCGTCTGGTTCGCCGACAACGGCGAGCGCCTCGGGACCTACCGCGGCCACAACGGCGCCGTTTGGTGCTGCGATGTGTCCAGAGACTCCGCCCGCCTCATTACCGGCAGCGCCGACCAGACCGCCAAGCTGTGGAACGTTCAGTCCGGTCAGCAGCTTTTCACCTTCAATTTTGACTCGCCGGCGAGGGCTGTTGACTTCTCCGTCGGCGATAAGCTCGCCGTCATCACCACCGACCCCTTCATGGACGTTACGTCGGCGATTCACGTCAAGCGTATCGCCGGAGATCCCGAAGATCAGATTGGTGAATCGGCTCTGATACTCAAGGGACCTCAGGGAAGAATTAACAGAGCTGTTTGGGGCCCTCTGAACAGAACCATCATAAGCGCCGGAGAAGACGCAGTTATTCGCATTTGGGATTCTGAGACTGGTAAACTACTTAAGGAGAATGATCCGGAAGTGGGTCACAAAAAGACCATAACTTCGCTTGCGAAATCTGTGGATGGTTCACATTTCCTCACCGGTTCGCTAGATAAATCTGCCAAGCTGTGGGATACTAGAACGTTGACTCTTATCAAGACCTATGTGACAGAGCGTCCTGTCAATGCCGTTGCAATGTCTCCCCTTTGTGATCATGTGGTACTTGGTGGTGGTCAGGATGCATCAACTGTCACCACAACTGATCATCGTTCTGGGAAATTTGAAGCCAAATTCTATGACAAGATTCTTCAAGAAGAAATTGGAGGTGTTAAAGGGCATTTTGGACCTATAAATGCTTTGGCGTTTAATCCCGATGGAAAAAGTTTCTCAAGCGGAGGTGAGGATGGTTACGTACGTTTACATCACTTTGATTCTGATTACTTCCACATCAAGATTTAG

Protein Analysis

446

Amino Acids

49.59

Weight (kDa)

8.56

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 120 - 444 7.5e-127 EIF3I
Beta-prop_TEP1_2nd PF25047 123 - 292 1.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 123 - 209 4.6e-15 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 124 - 292 5.4e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 125 - 226 8.9e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 125 - 210 2.6e-07 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 125 - 205 2.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 126 - 200 1.9e-09 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 127 - 240 2.8e-17 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 128 - 300 3.1e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 130 - 228 1.2e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 133 - 199 2.9e-07 G protein beta WD-40 repeat protein
WD40 PF00400 161 - 199 1.2e-09 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 169 - 294 8e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 255 - 371 1.3e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 260 - 384 2.4e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 261 - 434 1.1e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 264 - 386 2.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 268 - 390 5.1e-16 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 276 - 438 2.3e-08 WDHD1 first WD40 domain
WD40_Gbeta PF25391 279 - 383 2e-10 G protein beta WD-40 repeat protein
Beta-prop_IFT122_1st PF23381 280 - 367 8.2e-06 IFT122 first beta-propeller
WDR55 PF24796 307 - 386 5.2e-06 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 308 - 366 8.8e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 308 - 338 6.6e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 315 - 442 6.2e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 402 - 433 1.4e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 515
AccB7I CCANNNNNTGG 4 cut(s) 295, 465, 591, 1008
AccII CGCG 1 cut(s) 504
AccIII TCCGGA 1 cut(s) 913
AciI CCGC 7 cut(s) 4, 502, 504, 549, 553, 582, 1272
AclI AACGTT 2 cut(s) 597, 1022
AclWI GGATC 2 cut(s) 740, 905
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 3 cut(s) 191, 1157, 1169
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 2 cut(s) 1101, 1293
AfiI CCNNNNNNNGG 7 cut(s) 36, 295, 465, 591, 703, 920, 1008
AflII CTTAAG 1 cut(s) 899
AgeI ACCGGT 1 cut(s) 980
AgsI TTSAA 5 cut(s) 34, 102, 628, 1163, 1190
AhdI GACNNNNNGTC 2 cut(s) 712, 1053
AhlI ACTAGT 1 cut(s) 113
AjiI CACGTC 1 cut(s) 727
AjnI CCWGG 1 cut(s) 294
AjuI GAANNNNNNNTTGG 2 cut(s) 744, 776
AloI GAACNNNNNNTCC 2 cut(s) 587, 619
AluBI AGCT 6 cut(s) 175, 589, 616, 676, 812, 1006
AluI AGCT 6 cut(s) 175, 589, 616, 676, 812, 1006
Alw26I GTCTC 3 cut(s) 537, 878, 1083
AlwI GGATC 2 cut(s) 740, 905
Ama87I CYCGRG 2 cut(s) 142, 491
Aor13HI TCCGGA 1 cut(s) 913
AoxI GGCC 6 cut(s) 27, 184, 362, 389, 505, 820
ApaI GGGCCC 1 cut(s) 824
ApeKI GCWGC 5 cut(s) 172, 175, 528, 567, 613
ApoI RAATTY 3 cut(s) 191, 1157, 1169
ArsI GACNNNNNNTTYG 4 cut(s) 313, 345, 925, 957
AsiGI ACCGGT 1 cut(s) 980
AspLEI GCGC 5 cut(s) 443, 489, 518, 572, 848
AspS9I GGNCC 8 cut(s) 184, 390, 448, 495, 788, 820, 821, 1223
AsuC2I CCSGG 2 cut(s) 143, 144
AsuHPI GGTGA 8 cut(s) 58, 434, 613, 679, 776, 970, 1123, 1289
AvaI CYCGRG 2 cut(s) 142, 491
AvaII GGWCC 4 cut(s) 448, 495, 788, 1223
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 2 cut(s) 384, 824
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 824
BauI CACGAG 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 861
BbvI GCAGC 5 cut(s) 162, 184, 515, 579, 625
BccI CCATC 4 cut(s) 845, 956, 1247, 1277
BceAI ACGGC 6 cut(s) 267, 496, 503, 529, 665, 1052
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 1006
BclI TGATCA 2 cut(s) 1090, 1141
BcnI CCSGG 2 cut(s) 143, 144
BcoDI GTCTC 3 cut(s) 537, 878, 1083
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 3 cut(s) 114, 989, 1017
BfoI RGCGCY 4 cut(s) 490, 519, 573, 849
BfrI CTTAAG 1 cut(s) 899
BfuI GTATCC 1 cut(s) 1006
BglI GCCNNNNNGGC 1 cut(s) 513
BisI GCNGC 6 cut(s) 173, 176, 505, 529, 568, 614
BlsI GCNGC 6 cut(s) 174, 177, 506, 530, 569, 615
Bme1390I CCNGG 3 cut(s) 143, 144, 296
Bme18I GGWCC 4 cut(s) 448, 495, 788, 1223
BmeRI GACNNNNNGTC 2 cut(s) 712, 1053
BmeT110I CYCGRG 2 cut(s) 142, 491
BmgBI CACGTC 1 cut(s) 727
BmgT120I GGNCC 8 cut(s) 184, 390, 448, 495, 788, 820, 821, 1223
BmiI GGNNCC 5 cut(s) 496, 517, 789, 821, 822
BmrFI CCNGG 3 cut(s) 143, 144, 296
BmsI GCATC 2 cut(s) 1108, 1130
BpiI GAAGAC 1 cut(s) 861
BpuEI CTTGAG 2 cut(s) 767, 1252
BpuMI CCSGG 2 cut(s) 143, 144
BsaAI YACGTR 1 cut(s) 1291
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 6 cut(s) 142, 294, 295, 444, 490, 502
BsaWI WCCGGW 3 cut(s) 605, 913, 980
Bsc4I CCNNNNNNNGG 7 cut(s) 36, 295, 465, 591, 703, 920, 1008
Bse118I RCCGGY 3 cut(s) 563, 640, 980
Bse1I ACTGG 1 cut(s) 892
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 1080
BseAI TCCGGA 1 cut(s) 913
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 6 cut(s) 142, 294, 295, 444, 490, 502
BseGI GGATG 3 cut(s) 967, 1123, 1288
BseLI CCNNNNNNNGG 7 cut(s) 36, 295, 465, 591, 703, 920, 1008
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 2 cut(s) 806, 873
BseNI ACTGG 1 cut(s) 892
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 2 cut(s) 384, 824
BseXI GCAGC 5 cut(s) 162, 184, 515, 579, 625
BsgI GTGCAG 2 cut(s) 62, 296
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 6 cut(s) 29, 186, 364, 391, 507, 822
BshNI GGYRCC 1 cut(s) 515
BshTI ACCGGT 1 cut(s) 980
BsiHKCI CYCGRG 2 cut(s) 142, 491
BsiSI CCGG 8 cut(s) 143, 564, 606, 641, 741, 849, 914, 981
BsiWI CGTACG 1 cut(s) 1291
BslFI GGGAC 3 cut(s) 431, 508, 801
BslI CCNNNNNNNGG 7 cut(s) 36, 295, 465, 591, 703, 920, 1008
BsmAI GTCTC 3 cut(s) 537, 878, 1083
BsmFI GGGAC 3 cut(s) 431, 508, 801
BsnI GGCC 6 cut(s) 29, 186, 364, 391, 507, 822
BsoBI CYCGRG 2 cut(s) 142, 491
Bsp120I GGGCCC 1 cut(s) 820
Bsp1286I GDGCHC 2 cut(s) 384, 824
Bsp13I TCCGGA 1 cut(s) 913
Bsp143I GATC 5 cut(s) 745, 754, 910, 1090, 1141
BspACI CCGC 7 cut(s) 4, 502, 504, 549, 553, 582, 1272
BspANI GGCC 6 cut(s) 29, 186, 364, 391, 507, 822
BspCNI CTCAG 2 cut(s) 805, 874
BspEI TCCGGA 1 cut(s) 913
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 5 cut(s) 496, 517, 789, 821, 822
BspPI GGATC 2 cut(s) 740, 905
BspT107I GGYRCC 1 cut(s) 515
BspTI CTTAAG 1 cut(s) 899
BsrDI GCAATG 1 cut(s) 1080
BsrFI RCCGGY 3 cut(s) 563, 640, 980
BsrI ACTGG 1 cut(s) 892
BssAI RCCGGY 3 cut(s) 563, 640, 980
BssECI CCNNGG 6 cut(s) 142, 294, 295, 444, 490, 502
BssMI GATC 5 cut(s) 745, 754, 910, 1090, 1141
BssNI GRCGYC 1 cut(s) 516
BssSI CACGAG 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 444
Bst2BI CACGAG 1 cut(s) 383
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 5 cut(s) 24, 91, 422, 463, 1129
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 1 cut(s) 516
BstAFI CTTAAG 1 cut(s) 899
BstBAI YACGTR 1 cut(s) 1291
BstC8I GCNNGC 6 cut(s) 6, 485, 553, 642, 678, 948
BstDEI CTNAG 2 cut(s) 792, 882
BstDSI CCRYGG 1 cut(s) 502
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 967, 1123, 1288
BstFNI CGCG 1 cut(s) 504
BstH2I RGCGCY 4 cut(s) 490, 519, 573, 849
BstHHI GCGC 5 cut(s) 443, 489, 518, 572, 848
BstKTI GATC 5 cut(s) 748, 757, 913, 1093, 1144
BstMAI GTCTC 3 cut(s) 537, 878, 1083
BstMBI GATC 5 cut(s) 745, 754, 910, 1090, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 388, 513
BstNI CCWGG 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 3 cut(s) 141, 142, 294
BstSLI GKGCMC 2 cut(s) 384, 824
BstSNI TACGTA 1 cut(s) 1291
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 5 cut(s) 162, 184, 515, 579, 625
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 1 cut(s) 1006
BsuRI GGCC 6 cut(s) 29, 186, 364, 391, 507, 822
BtgI CCRYGG 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 3 cut(s) 967, 1123, 1288
Cac8I GCNNGC 6 cut(s) 6, 485, 553, 642, 678, 948
CfoI GCGC 5 cut(s) 443, 489, 518, 572, 848
Cfr10I RCCGGY 3 cut(s) 563, 640, 980
Cfr13I GGNCC 8 cut(s) 184, 390, 448, 495, 788, 820, 821, 1223
Cfr42I CCGCGG 1 cut(s) 505
Cfr9I CCCGGG 1 cut(s) 142
CseI GACGC 2 cut(s) 866, 1043
Csp6I GTAC 2 cut(s) 1100, 1292
CspAI ACCGGT 1 cut(s) 980
CviAII CATG 4 cut(s) 164, 168, 703, 1094
CviQI GTAC 2 cut(s) 1100, 1292
DdeI CTNAG 2 cut(s) 792, 882
DinI GGCGCC 1 cut(s) 517
DpnI GATC 5 cut(s) 747, 756, 912, 1092, 1143
DpnII GATC 5 cut(s) 745, 754, 910, 1090, 1141
DrdI GACNNNNNNGTC 1 cut(s) 662
DriI GACNNNNNGTC 2 cut(s) 712, 1053
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 437
Eam1105I GACNNNNNGTC 2 cut(s) 712, 1053
EarI CTCTTC 1 cut(s) 437
EciI GGCGGA 1 cut(s) 538
Eco105I TACGTA 1 cut(s) 1291
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 824
Eco47I GGWCC 4 cut(s) 448, 495, 788, 1223
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 2 cut(s) 142, 491
Eco91I GGTNACC 1 cut(s) 422
EcoO109I RGGNCCY 5 cut(s) 390, 495, 788, 820, 821
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 2 cut(s) 169, 1123
EcoT38I GRGCYC 1 cut(s) 824
EgeI GGCGCC 1 cut(s) 517
EheI GGCGCC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 4 cut(s) 167, 171, 706, 1097
FaqI GGGAC 3 cut(s) 431, 508, 801
FatI CATG 4 cut(s) 163, 167, 702, 1093
FauI CCCGC 1 cut(s) 560
FbaI TGATCA 2 cut(s) 1090, 1141
Fnu4HI GCNGC 6 cut(s) 173, 176, 505, 529, 568, 614
FokI GGATG 3 cut(s) 974, 1130, 1295
FriOI GRGCYC 1 cut(s) 824
Fsp4HI GCNGC 6 cut(s) 173, 176, 505, 529, 568, 614
FspBI CTAG 3 cut(s) 114, 989, 1017
GlaI GCGC 5 cut(s) 442, 488, 517, 571, 847
GluI GCNGC 6 cut(s) 173, 176, 505, 529, 568, 614
HaeII RGCGCY 4 cut(s) 490, 519, 573, 849
HaeIII GGCC 6 cut(s) 29, 186, 364, 391, 507, 822
HapII CCGG 8 cut(s) 143, 564, 606, 641, 741, 849, 914, 981
HgaI GACGC 2 cut(s) 866, 1043
HhaI GCGC 5 cut(s) 443, 489, 518, 572, 848
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 4 cut(s) 167, 171, 706, 1097
Hin6I GCGC 5 cut(s) 441, 487, 516, 570, 846
HinP1I GCGC 5 cut(s) 441, 487, 516, 570, 846
HincII GTYRAC 3 cut(s) 58, 655, 1026
HindII GTYRAC 3 cut(s) 58, 655, 1026
HpaII CCGG 8 cut(s) 143, 564, 606, 641, 741, 849, 914, 981
HphI GGTGA 8 cut(s) 58, 434, 613, 679, 776, 970, 1123, 1289
Hpy166II GTNNAC 7 cut(s) 20, 58, 655, 893, 968, 1026, 1298
Hpy188I TCNGA 7 cut(s) 348, 354, 759, 777, 828, 883, 1315
Hpy8I GTNNAC 7 cut(s) 20, 58, 655, 893, 968, 1026, 1298
Hpy99I CGWCG 5 cut(s) 288, 333, 358, 668, 718
HpyAV CCTTC 3 cut(s) 40, 634, 709
HpyCH4III ACNGT 5 cut(s) 24, 91, 422, 463, 1129
HpyCH4IV ACGT 8 cut(s) 276, 597, 708, 713, 726, 1022, 1290, 1294
HpyCH4V TGCA 5 cut(s) 79, 167, 313, 1073, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 388, 513
HpyF3I CTNAG 2 cut(s) 792, 882
HpySE526I ACGT 8 cut(s) 276, 597, 708, 713, 726, 1022, 1290, 1294
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 4 cut(s) 167, 171, 706, 1097
HspAI GCGC 5 cut(s) 441, 487, 516, 570, 846
KasI GGCGCC 1 cut(s) 515
Kpn2I TCCGGA 1 cut(s) 913
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 2 cut(s) 1090, 1141
KspI CCGCGG 1 cut(s) 505
Kzo9I GATC 5 cut(s) 745, 754, 910, 1090, 1141
Lsp1109I GCAGC 5 cut(s) 162, 184, 515, 579, 625
LweI GCATC 2 cut(s) 1108, 1130
MaeI CTAG 3 cut(s) 114, 989, 1017
MaeII ACGT 8 cut(s) 276, 597, 708, 713, 726, 1022, 1290, 1294
MaeIII GTNAC 6 cut(s) 422, 709, 923, 1045, 1129, 1286
MalI GATC 5 cut(s) 747, 756, 912, 1092, 1143
MboI GATC 5 cut(s) 745, 754, 910, 1090, 1141
MboII GAAGA 8 cut(s) 95, 244, 424, 764, 810, 866, 1178, 1205
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 2 cut(s) 384, 824
MluCI AATT 6 cut(s) 191, 628, 801, 1157, 1169, 1197
Mly113I GGCGCC 1 cut(s) 516
MlyI GAGTC 4 cut(s) 343, 539, 629, 1021
MmeI TCCRAC 3 cut(s) 98, 371, 377
Mph1103I ATGCAT 2 cut(s) 169, 1123
MreI CGCCGGCG 1 cut(s) 640
MroI TCCGGA 1 cut(s) 913
MroNI GCCGGC 1 cut(s) 640
MseI TTAA 7 cut(s) 189, 218, 237, 804, 900, 1209, 1245
MspA1I CMGCKG 2 cut(s) 175, 504
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 8 cut(s) 143, 564, 606, 641, 741, 849, 914, 981
MspR9I CCNGG 3 cut(s) 143, 144, 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 388, 513
NaeI GCCGGC 1 cut(s) 642
NarI GGCGCC 1 cut(s) 516
NciI CCSGG 2 cut(s) 143, 144
NdeII GATC 5 cut(s) 745, 754, 910, 1090, 1141
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 4 cut(s) 167, 171, 706, 1097
NlaIV GGNNCC 5 cut(s) 496, 517, 789, 821, 822
NmuCI GTSAC 4 cut(s) 422, 923, 1045, 1129
NsiI ATGCAT 2 cut(s) 169, 1123
PasI CCCWGGG 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PdiI GCCGGC 1 cut(s) 642
PfeI GAWTC 8 cut(s) 300, 307, 333, 721, 767, 878, 1183, 1310
Pfl23II CGTACG 1 cut(s) 1291
PflMI CCANNNNNTGG 4 cut(s) 295, 465, 591, 1008
PinAI ACCGGT 1 cut(s) 980
PkrI GCNGC 6 cut(s) 174, 177, 506, 530, 569, 615
PleI GAGTC 4 cut(s) 343, 539, 629, 1021
PluTI GGCGCC 1 cut(s) 519
PpsI GAGTC 4 cut(s) 343, 539, 629, 1021
Ppu21I YACGTR 1 cut(s) 1291
PpuMI RGGWCCY 2 cut(s) 495, 788
Psp1406I AACGTT 2 cut(s) 597, 1022
Psp5II RGGWCCY 2 cut(s) 495, 788
Psp6I CCWGG 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 1 cut(s) 294
PspLI CGTACG 1 cut(s) 1291
PspN4I GGNNCC 5 cut(s) 496, 517, 789, 821, 822
PspOMI GGGCCC 1 cut(s) 820
PspPI GGNCC 8 cut(s) 184, 390, 448, 495, 788, 820, 821, 1223
PspPPI RGGWCCY 2 cut(s) 495, 788
PsuI RGATCY 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 2 cut(s) 1101, 1293
RsaNI GTAC 2 cut(s) 1100, 1292
SacII CCGCGG 1 cut(s) 505
SaqAI TTAA 7 cut(s) 189, 218, 237, 804, 900, 1209, 1245
SatI GCNGC 6 cut(s) 173, 176, 505, 529, 568, 614
Sau3AI GATC 5 cut(s) 745, 754, 910, 1090, 1141
Sau96I GGNCC 8 cut(s) 184, 390, 448, 495, 788, 820, 821, 1223
SchI GAGTC 4 cut(s) 343, 539, 629, 1021
ScrFI CCNGG 3 cut(s) 143, 144, 296
SduI GDGCHC 2 cut(s) 384, 824
SfaNI GCATC 2 cut(s) 1108, 1130
SfoI GGCGCC 1 cut(s) 517
Sfr303I CCGCGG 1 cut(s) 505
SgrAI CRCCGGYG 1 cut(s) 640
SgrBI CCGCGG 1 cut(s) 505
SinI GGWCC 4 cut(s) 448, 495, 788, 1223
SmaI CCCGGG 1 cut(s) 144
SmlI CTYRAG 3 cut(s) 782, 899, 1267
SmoI CTYRAG 3 cut(s) 782, 899, 1267
SnaBI TACGTA 1 cut(s) 1291
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 6 cut(s) 191, 628, 801, 1157, 1169, 1197
SsiI CCGC 7 cut(s) 4, 502, 504, 549, 553, 582, 1272
SspDI GGCGCC 1 cut(s) 515
SspMI CTAG 3 cut(s) 114, 989, 1017
StyD4I CCNGG 3 cut(s) 141, 142, 294
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 5 cut(s) 24, 91, 422, 463, 1129
TaiI ACGT 8 cut(s) 279, 600, 711, 716, 729, 1025, 1293, 1297
TaqI TCGA 1 cut(s) 331
TasI AATT 6 cut(s) 191, 628, 801, 1157, 1169, 1197
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 8 cut(s) 300, 307, 333, 721, 767, 878, 1183, 1310
Tru1I TTAA 7 cut(s) 189, 218, 237, 804, 900, 1209, 1245
Tru9I TTAA 7 cut(s) 189, 218, 237, 804, 900, 1209, 1245
TseFI GTSAC 4 cut(s) 422, 923, 1045, 1129
TseI GCWGC 5 cut(s) 172, 175, 528, 567, 613
Tsp45I GTSAC 4 cut(s) 422, 923, 1045, 1129
TspDTI ATGAA 2 cut(s) 389, 691
TspGWI ACGGA 2 cut(s) 317, 652
TspMI CCCGGG 1 cut(s) 142
Van91I CCANNNNNTGG 4 cut(s) 295, 465, 591, 1008
Vha464I CTTAAG 1 cut(s) 899
VpaK11BI GGWCC 4 cut(s) 448, 495, 788, 1223
XapI RAATTY 3 cut(s) 191, 1157, 1169
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XmaI CCCGGG 1 cut(s) 142
XspI CTAG 3 cut(s) 114, 989, 1017
Zsp2I ATGCAT 2 cut(s) 169, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.