pycom13g14160

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009678
Physical Location & Seq
Reverse (-)
10138961 .. 10139293
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14160.2

Sequence Viewer

Length: 231 bp
ATGGTGACTAATTACTTTGAGACCATATTTCGATCTTGTGCACTACCGAACGATAATTCCGATTTGGTTGAGGATATCAAATGCAAAGTTCTTGATGCTATGAATCTGTCTCTTATCTCTCAAGTTACCACGGAGGAAGTCCGAGCTGCACTTTTCCAAATGCACCCATCTAAGTCCCCGGGACCGAACAGAATGCCTCCGATATTCTTCCAACGACCGAAACAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

77

Amino Acids

8.64

Weight (kDa)

6.54

Isoelectric Point (pI)

62.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
AluBI AGCT 1 cut(s) 146
AluI AGCT 1 cut(s) 146
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 2 cut(s) 14, 114
Alw44I GTGCAC 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 178
ApaLI GTGCAC 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 182
AsuC2I CCSGG 2 cut(s) 179, 180
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BcnI CCSGG 2 cut(s) 179, 180
BcoDI GTCTC 2 cut(s) 14, 114
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 179, 180
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 2 cut(s) 179, 180
BmsI GCATC 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 105
BpuMI CCSGG 2 cut(s) 179, 180
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 129, 177, 178
BseDI CCNNGG 3 cut(s) 129, 177, 178
BseSI GKGCMC 1 cut(s) 43
BseXI GCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 2 cut(s) 160, 195
BsmAI GTCTC 2 cut(s) 14, 114
BsmFI GGGAC 2 cut(s) 160, 195
BsmI GAATGC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 14
BssECI CCNNGG 3 cut(s) 129, 177, 178
BssMI GATC 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 1 cut(s) 129
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 2 cut(s) 14, 114
BstMBI GATC 1 cut(s) 32
BstMCI CGRYCG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 177, 178
BstSLI GKGCMC 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 133
BtgI CCRYGG 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 182
Cfr9I CCCGGG 1 cut(s) 178
CviJI RGCY 1 cut(s) 146
CviKI_1 RGCY 1 cut(s) 146
DdeI CTNAG 1 cut(s) 171
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 178
EcoRV GATATC 1 cut(s) 76
FaiI YATR 2 cut(s) 26, 101
FaqI GGGAC 2 cut(s) 160, 195
Fnu4HI GCNGC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
HapII CCGG 1 cut(s) 179
HinfI GANTC 1 cut(s) 103
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 3 cut(s) 61, 143, 201
Hpy188III TCNNGA 2 cut(s) 92, 228
Hpy8I GTNNAC 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4V TGCA 4 cut(s) 41, 84, 149, 163
HpyF3I CTNAG 1 cut(s) 171
Kzo9I GATC 1 cut(s) 32
LpnPI CCDG 1 cut(s) 192
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 1 cut(s) 85
MaeIII GTNAC 2 cut(s) 4, 124
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 2 cut(s) 10, 55
MnlI CCTC 3 cut(s) 64, 127, 207
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 179, 180
Mva1269I GAATGC 1 cut(s) 198
NciI CCSGG 2 cut(s) 179, 180
NdeII GATC 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 4
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 182
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 179, 180
SduI GDGCHC 1 cut(s) 43
SetI ASST 1 cut(s) 148
SfaNI GCATC 1 cut(s) 85
SgeI CNNG 8 cut(s) 48, 104, 134, 142, 155, 190, 191, 192
SinI GGWCC 1 cut(s) 182
SmaI CCCGGG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 2 cut(s) 10, 55
StyD4I CCNGG 2 cut(s) 177, 178
TaaI ACNGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 1 cut(s) 199
TasI AATT 2 cut(s) 10, 55
TfiI GAWTC 1 cut(s) 103
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 116
TspGWI ACGGA 1 cut(s) 146
TspMI CCCGGG 1 cut(s) 178
VneI GTGCAC 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.