Rmu_sc0010800.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010800.1
OG0009678
Physical Location & Seq
Forward (+)
1058 .. 2193
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010800.1_g000001.1.cds

Sequence Viewer

Length: 537 bp
atgaagtcccagtccatattcatcattattctgcacgacatattgatgcatggattggagcaccaggagatccggaccgatggcggttcactggaactgcagactatggaggatgattattggagacaacgcagtaaggttgattggctaagggatggagataggaataccgcctactttcatcgccgagcatcaaataggaaacatcgtaacgccattagtggtttatataatgcagaggggcagtgggttactagctcacatgaagttgaggacgttattacacattactataataatctcttctcttctcaaggggtggatgcagcagcattaactattatcctggatacggtttccccaaaggtgaatgcatcgatgaaccgaaagctgttggcgccttatacggatgaagaggttcgaacaacagtattccagatgcatccatcaaaggcacctggcccggatgggatgtcccctttcttttatcagaagttttggaaaacagttgaagtattatgtcattaccaaatatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

178

Amino Acids

20.65

Weight (kDa)

6.29

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 397, 454
AccIII TCCGGA 1 cut(s) 72
AciI CCGC 2 cut(s) 84, 171
AclWI GGATC 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 1 cut(s) 512
AjnI CCWGG 3 cut(s) 63, 345, 457
AluBI AGCT 2 cut(s) 258, 391
AluI AGCT 2 cut(s) 258, 391
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 64
Aor13HI TCCGGA 1 cut(s) 72
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 326, 329
Asp700I GAANNNNTTC 1 cut(s) 417
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 2 cut(s) 75, 461
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 379
AsuII TTCGAA 1 cut(s) 421
AvaII GGWCC 1 cut(s) 75
BanI GGYRCC 2 cut(s) 397, 454
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 338, 341
BccI CCATC 4 cut(s) 74, 149, 454, 461
BciT130I CCWGG 3 cut(s) 65, 347, 459
BciVI GTATCC 1 cut(s) 343
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 401
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 2 cut(s) 327, 330
BlsI GCNGC 2 cut(s) 328, 331
Bme1390I CCNGG 4 cut(s) 65, 347, 459, 464
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 2 cut(s) 75, 461
BmiI GGNNCC 2 cut(s) 399, 456
BmrFI CCNGG 4 cut(s) 65, 347, 459, 464
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 6 cut(s) 36, 200, 313, 383, 429, 451
BmuI ACTGGG 1 cut(s) 4
Bpu10I CCTNAGC 1 cut(s) 149
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 464
Bsa29I ATCGAT 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 10, 96
BseAI TCCGGA 1 cut(s) 72
BseBI CCWGG 3 cut(s) 65, 347, 459
BseCI ATCGAT 1 cut(s) 377
BseGI GGATG 7 cut(s) 118, 160, 328, 415, 442, 472, 477
BseLI CCNNNNNNNGG 1 cut(s) 352
BseNI ACTGG 2 cut(s) 10, 96
BseXI GCAGC 2 cut(s) 338, 341
BsgI GTGCAG 1 cut(s) 17
BshFI GGCC 1 cut(s) 462
BshNI GGYRCC 2 cut(s) 397, 454
BshVI ATCGAT 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 63
BsiSI CCGG 2 cut(s) 73, 464
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 1 cut(s) 462
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 63
Bsp13I TCCGGA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 2 cut(s) 84, 171
BspANI GGCC 1 cut(s) 462
BspDI ATCGAT 1 cut(s) 377
BspEI TCCGGA 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 399, 456
BspMAI CTGCAG 1 cut(s) 102
BspPI GGATC 1 cut(s) 64
BspT104I TTCGAA 1 cut(s) 421
BspT107I GGYRCC 2 cut(s) 397, 454
BsrI ACTGG 2 cut(s) 10, 96
BssMI GATC 1 cut(s) 69
BssNI GRCGYC 1 cut(s) 398
Bst2UI CCWGG 3 cut(s) 65, 347, 459
Bst4CI ACNGT 3 cut(s) 355, 430, 508
Bst6I CTCTTC 3 cut(s) 308, 313, 408
BstACI GRCGYC 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 421
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 7 cut(s) 118, 160, 328, 415, 442, 472, 477
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 397
BstNI CCWGG 3 cut(s) 65, 347, 459
BstSCI CCNGG 4 cut(s) 63, 345, 457, 462
BstSFI CTRYAG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 338, 341
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 377
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 7 cut(s) 118, 160, 328, 415, 442, 472, 477
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 2 cut(s) 89, 251
CfoI GCGC 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 75, 461
ClaI ATCGAT 1 cut(s) 377
CpoI CGGWCCG 1 cut(s) 75
CspI CGGWCCG 1 cut(s) 75
CviAII CATG 2 cut(s) 50, 263
CviJI RGCY 4 cut(s) 148, 258, 391, 462
CviKI_1 RGCY 4 cut(s) 148, 258, 391, 462
DdeI CTNAG 1 cut(s) 149
DinI GGCGCC 1 cut(s) 399
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
Eam1104I CTCTTC 3 cut(s) 308, 313, 408
EarI CTCTTC 3 cut(s) 308, 313, 408
Eco47I GGWCC 1 cut(s) 75
EcoRII CCWGG 3 cut(s) 63, 345, 457
EcoT22I ATGCAT 3 cut(s) 51, 376, 444
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
FaeI CATG 2 cut(s) 53, 266
FaqI GGGAC 1 cut(s) 460
FatI CATG 2 cut(s) 49, 262
Fnu4HI GCNGC 2 cut(s) 327, 330
FokI GGATG 7 cut(s) 125, 167, 335, 422, 429, 479, 484
Fsp4HI GCNGC 2 cut(s) 327, 330
FspBI CTAG 1 cut(s) 255
GlaI GCGC 1 cut(s) 399
GluI GCNGC 2 cut(s) 327, 330
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 1 cut(s) 462
HapII CCGG 2 cut(s) 73, 464
HhaI GCGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 2 cut(s) 53, 266
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HpaII CCGG 2 cut(s) 73, 464
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 2 cut(s) 73, 436
Hpy8I GTNNAC 1 cut(s) 89
HpyCH4III ACNGT 3 cut(s) 355, 430, 508
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 7 cut(s) 34, 49, 100, 236, 326, 374, 442
HpyF10VI GCNNNNNNNGC 1 cut(s) 397
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 2 cut(s) 53, 266
HspAI GCGC 1 cut(s) 398
KasI GGCGCC 1 cut(s) 397
Kpn2I TCCGGA 1 cut(s) 72
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 338, 341
LweI GCATC 6 cut(s) 36, 200, 313, 383, 429, 451
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 209, 250
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 3 cut(s) 295, 300, 425
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 63
Mly113I GGCGCC 1 cut(s) 398
MnlI CCTC 4 cut(s) 103, 232, 265, 409
Mph1103I ATGCAT 3 cut(s) 51, 376, 444
MroI TCCGGA 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 2 cut(s) 73, 464
MspR9I CCNGG 4 cut(s) 65, 347, 459, 464
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 3 cut(s) 65, 347, 459
MwoI GCNNNNNNNGC 1 cut(s) 397
NarI GGCGCC 1 cut(s) 398
NciI CCSGG 1 cut(s) 464
NdeII GATC 1 cut(s) 69
NlaIII CATG 2 cut(s) 53, 266
NlaIV GGNNCC 2 cut(s) 399, 456
NmeAIII GCCGAG 1 cut(s) 212
NsiI ATGCAT 3 cut(s) 51, 376, 444
NspV TTCGAA 1 cut(s) 421
PctI GAATGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 2 cut(s) 328, 331
PluTI GGCGCC 1 cut(s) 401
Psp6I CCWGG 3 cut(s) 63, 345, 457
PspGI CCWGG 3 cut(s) 63, 345, 457
PspN4I GGNNCC 2 cut(s) 399, 456
PspPI GGNCC 2 cut(s) 75, 461
PstI CTGCAG 1 cut(s) 102
PsuI RGATCY 1 cut(s) 69
RseI CAYNNNNRTG 1 cut(s) 44
Rsr2I CGGWCCG 1 cut(s) 75
RsrII CGGWCCG 1 cut(s) 75
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 327, 330
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 2 cut(s) 75, 461
ScrFI CCNGG 4 cut(s) 65, 347, 459, 464
SduI GDGCHC 1 cut(s) 63
SetI ASST 7 cut(s) 141, 260, 279, 369, 393, 420, 460
SfaNI GCATC 6 cut(s) 36, 200, 313, 383, 429, 451
SfcI CTRYAG 1 cut(s) 98
SfoI GGCGCC 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 421
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SsiI CCGC 2 cut(s) 84, 171
SspDI GGCGCC 1 cut(s) 397
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 4 cut(s) 63, 345, 457, 462
TaaI ACNGT 3 cut(s) 355, 430, 508
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 377, 421
TaqII GACCGA 1 cut(s) 92
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 96, 251
TseI GCWGC 2 cut(s) 326, 329
TspDTI ATGAA 6 cut(s) 10, 17, 170, 279, 395, 426
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 2 cut(s) 96, 251
VpaK11BI GGWCC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 1 cut(s) 255
Zsp2I ATGCAT 3 cut(s) 51, 376, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.