pycom13g16850

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005475
Physical Location & Seq
Forward (+)
12569536 .. 12570587
1052 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGATAACCACTGCCACCGCTCCTCCGCCCCTCATGGCCATCCTCCTCGCCTTGACGCTCGCCATTATCAGCACCGAAGCAGCAGAACCGCCGTCGCCGCTACCCAGTTGTGGTGACGAGCTCGTGAGATTCTCGCCGTGCCTGCCGTACGTGTCGTCTCCGCCGAACAACCTCTCCGACTCGCCCCCACCCAAGTGCTGCGACGCGTTCTCGCTGTCGCTGGAGTCCGGTGGCGCTCTGTGCCTCTGCTACCTGGTCCAGGATCCTCCTATGCTCGGGTTTCCCGTGAACGGGAGTCGCGTTCTGTTTCTGTCTTCCACTTGCCCTCTCGGAGACATTTCCACAAACACAAGCAGTGCAGAGTCTTTGGAATCGCTCTGCTCAGGATCGCCAGAACTCCCTCCTCTCCGCAGCTCAACAATTTCAGAGATTTCTCCTCCTCCTTCTGGTTTTGAGTCTGTTGGCAATGCTTCATCTCCTCTCATGAGCTTAGCACCAGAATCAGCAAACACTACAAGCATTCCACCGGGAAACAGATCTCCGACTCCACCAAGCTCAGCGGTATATCCGGCTAGGGTTTCAGCAGCAGTGAAGCAAATTCACACAAGCAACACTTGGTTTCTACCTGCCCTACTGAGTTTCCTTGTTTCCATGTCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

221

Amino Acids

22.63

Weight (kDa)

4.39

Isoelectric Point (pI)

78.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 21 - 110 3.7e-12 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 634
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 2 cut(s) 206, 300
AciI CCGC 7 cut(s) 18, 26, 89, 98, 161, 409, 560
AclWI GGATC 3 cut(s) 257, 270, 394
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 597
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 6 cut(s) 110, 259, 275, 290, 291, 446
AflIII ACRYGT 2 cut(s) 150, 204
AjnI CCWGG 2 cut(s) 252, 258
AleI CACNNNNGTG 1 cut(s) 193
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 5 cut(s) 121, 414, 489, 555, 660
AluI AGCT 5 cut(s) 121, 414, 489, 555, 660
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 162, 327
AlwI GGATC 3 cut(s) 257, 270, 394
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 4 cut(s) 80, 198, 411, 584
ApoI RAATTY 1 cut(s) 597
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 123
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 92, 185, 423, 596
BccI CCATC 1 cut(s) 47
BceAI ACGGC 3 cut(s) 76, 121, 130
BciT130I CCWGG 2 cut(s) 254, 260
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 162, 327
BfaI CTAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 634
BglII AGATCT 1 cut(s) 536
BisI GCNGC 5 cut(s) 81, 98, 199, 412, 585
BlpI GCTNAGC 2 cut(s) 490, 556
BlsI GCNGC 5 cut(s) 82, 99, 200, 413, 586
Bme1390I CCNGG 3 cut(s) 254, 260, 528
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 3 cut(s) 254, 260, 528
BmrI ACTGGG 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 382
Bpu1102I GCTNAGC 2 cut(s) 490, 556
BpuMI CCSGG 1 cut(s) 528
BsaAI YACGTR 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 158, 188
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 259, 275, 290, 291, 446
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 472
BseBI CCWGG 2 cut(s) 254, 260
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 6 cut(s) 110, 259, 275, 290, 291, 446
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 3 cut(s) 396, 570, 626
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 6 cut(s) 12, 35, 393, 426, 429, 468
BseXI GCAGC 4 cut(s) 92, 185, 423, 596
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 2 cut(s) 206, 300
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 3 cut(s) 228, 527, 569
BsiWI CGTACG 1 cut(s) 147
BslI CCNNNNNNNGG 6 cut(s) 110, 259, 275, 290, 291, 446
BsmAI GTCTC 2 cut(s) 162, 327
BsmBI CGTCTC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 3 cut(s) 262, 386, 536
Bsp1720I GCTNAGC 2 cut(s) 490, 556
BspACI CCGC 7 cut(s) 18, 26, 89, 98, 161, 409, 560
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 3 cut(s) 395, 569, 627
BspFNI CGCG 2 cut(s) 206, 300
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 634
BspPI GGATC 3 cut(s) 257, 270, 394
BsrBI CCGCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 1 cut(s) 105
BssMI GATC 3 cut(s) 262, 386, 536
BssSI CACGAG 1 cut(s) 122
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 254, 260
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 60, 143
BstDEI CTNAG 4 cut(s) 382, 490, 556, 635
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 39
BstFNI CGCG 2 cut(s) 206, 300
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 3 cut(s) 265, 389, 539
BstMAI GTCTC 2 cut(s) 162, 327
BstMBI GATC 3 cut(s) 262, 386, 536
BstMWI GCNNNNNNNGC 3 cut(s) 97, 142, 240
BstNI CCWGG 2 cut(s) 254, 260
BstSCI CCNGG 3 cut(s) 252, 258, 526
BstUI CGCG 2 cut(s) 206, 300
BstV1I GCAGC 4 cut(s) 92, 185, 423, 596
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 2 cut(s) 262, 536
BstYI RGATCY 2 cut(s) 262, 536
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 39
BtsI GCAGTG 3 cut(s) 9, 361, 594
BtsIMutI CAGTG 3 cut(s) 9, 361, 594
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 60, 143
CciI TCATGA 1 cut(s) 483
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 256
CseI GACGC 2 cut(s) 64, 212
CsiI ACCWGGT 1 cut(s) 252
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 34, 484, 652
CviJI RGCY 7 cut(s) 38, 121, 414, 489, 555, 572, 660
CviKI_1 RGCY 7 cut(s) 38, 121, 414, 489, 555, 572, 660
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 4 cut(s) 382, 490, 556, 635
DpnI GATC 3 cut(s) 264, 388, 538
DpnII GATC 3 cut(s) 262, 386, 536
EaeI YGGCCR 1 cut(s) 36
EciI GGCGGA 2 cut(s) 15, 150
Ecl136II GAGCTC 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 252, 258
EcoT38I GRGCYC 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 3 cut(s) 37, 487, 655
FaiI YATR 5 cut(s) 35, 272, 485, 565, 653
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FatI CATG 3 cut(s) 33, 483, 651
Fnu4HI GCNGC 5 cut(s) 81, 98, 199, 412, 585
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 5 cut(s) 81, 98, 199, 412, 585
FspBI CTAG 1 cut(s) 573
GlaI GCGC 1 cut(s) 235
GluI GCNGC 5 cut(s) 81, 98, 199, 412, 585
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 1 cut(s) 38
HapII CCGG 3 cut(s) 228, 527, 569
HgaI GACGC 2 cut(s) 64, 212
HhaI GCGC 1 cut(s) 236
Hin1II CATG 3 cut(s) 37, 487, 655
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HinfI GANTC 9 cut(s) 129, 179, 224, 295, 362, 371, 455, 500, 544
HpaII CCGG 3 cut(s) 228, 527, 569
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 4 cut(s) 178, 332, 427, 543
Hpy188III TCNNGA 3 cut(s) 124, 384, 484
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 97, 206
HpyAV CCTTC 1 cut(s) 453
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 142, 240
HpyF3I CTNAG 4 cut(s) 382, 490, 556, 635
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 37, 487, 655
HspAI GCGC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 262, 386, 536
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 4 cut(s) 92, 185, 423, 596
MabI ACCWGGT 1 cut(s) 252
MaeI CTAG 1 cut(s) 573
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 113
MalI GATC 3 cut(s) 264, 388, 538
MbiI CCGCTC 1 cut(s) 20
MboI GATC 3 cut(s) 262, 386, 536
MboII GAAGA 1 cut(s) 306
MflI RGATCY 2 cut(s) 262, 536
MhlI GDGCHC 1 cut(s) 123
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 2 cut(s) 420, 597
MluI ACGCGT 1 cut(s) 204
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 6 cut(s) 173, 233, 304, 371, 464, 538
MmeI TCCRAC 2 cut(s) 201, 566
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 193
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 560, 660
MspI CCGG 3 cut(s) 228, 527, 569
MspR9I CCNGG 3 cut(s) 254, 260, 528
Mva1269I GAATGC 1 cut(s) 519
MvaI CCWGG 2 cut(s) 254, 260
MvnI CGCG 2 cut(s) 206, 300
MwoI GCNNNNNNNGC 3 cut(s) 97, 142, 240
NciI CCSGG 1 cut(s) 528
NdeII GATC 3 cut(s) 262, 386, 536
NlaIII CATG 3 cut(s) 37, 487, 655
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 113
OliI CACNNNNGTG 1 cut(s) 193
PagI TCATGA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 3 cut(s) 161, 282, 297
PctI GAATGC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 129, 371, 500
Pfl23II CGTACG 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 5 cut(s) 82, 99, 200, 413, 586
PleI GAGTC 6 cut(s) 173, 232, 303, 370, 463, 538
PpsI GAGTC 6 cut(s) 173, 232, 303, 370, 463, 538
Ppu21I YACGTR 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 252, 258
PspGI CCWGG 2 cut(s) 252, 258
PspLI CGTACG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 256
PsuI RGATCY 2 cut(s) 262, 536
PvuII CAGCTG 1 cut(s) 660
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 193
SacI GAGCTC 1 cut(s) 123
SatI GCNGC 5 cut(s) 81, 98, 199, 412, 585
Sau3AI GATC 3 cut(s) 262, 386, 536
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 6 cut(s) 173, 233, 304, 371, 464, 538
ScrFI CCNGG 3 cut(s) 254, 260, 528
SduI GDGCHC 1 cut(s) 123
SetI ASST 9 cut(s) 123, 153, 174, 255, 416, 491, 557, 628, 662
SexAI ACCWGGT 1 cut(s) 252
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 193
Sse9I AATT 2 cut(s) 420, 597
SsiI CCGC 7 cut(s) 18, 26, 89, 98, 161, 409, 560
SspMI CTAG 1 cut(s) 573
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 252, 258, 526
TaiI ACGT 1 cut(s) 153
TasI AATT 2 cut(s) 420, 597
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 3 cut(s) 129, 371, 500
TscAI CASTG 3 cut(s) 16, 361, 594
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 4 cut(s) 80, 198, 411, 584
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 462
TspRI CASTG 3 cut(s) 16, 361, 594
VpaK11BI GGWCC 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 597
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.