pycom13g17430

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004390
Physical Location & Seq
Forward (+)
13179169 .. 13182521
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGGATCCGGAGAGGATCCTTTTCCAATCGAACGCCACGTATCAGTCTGCGTTTTCACGGACGTCAGTAAACCAAAAACGCGAATGGTTTGAAAACCTACACATAGACCACCTTCCCCTCTCCTCCTCCTCCAACTTCAAGCTGTCGTCTTGGAGTTTGAATATTTCAGACCCGAAAACAGAAAAGACAAAAAAAAAAAAAAAAATGGAGGGAAGGGCGAGGGGTTTGACGCTGATTGGCCTTCTTTTGGTGAGCTTCGTCGGAAAGATCGCATCGCTATCGGTGACAGTGAACGACGTTGAATGCGTGTACGAGTACGTGCTTTACGAAGGCGATACCGTTTCGGGCAACTTCGTGGTCGTCGATCACGATATTTTCTGGGGCGCCGATCATCCCGGCCTCGATTTAATCGTGACATCTCCTGGGGATAATACGGTGCACACTTCAAAGGGAACATCTGGTGATAAGTTTGAGTTTAAAGCCCCAAGAAGCGGAATGTACAAATTTTGTTTTCACAATCCTTACTCAACACCAGAGACTGTTTCTTTTAACATACATGTTGGTCATATTCCCAACGAGCACAATATTGCCAAAGATGAACATCTAGACCCAATAAATGTTAAAATTGCTGAGCTTAGAGAAGCATTGGAGTCAGTTACATTAGAGCAGAAGTACTTGAAAGCACGTGATGTTCGACATCGTCATACAAATGAGAGCACAAGGAGGCGGGTTGTGTTGTATACAGTGGGAGAGTATCTTGCGCTAGCTGCTGCGAGTGCCTTGCAAGTCGTTTACATTCGCAAACTTTTCAGCAAATCGGTTGCATACAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

280

Amino Acids

31.58

Weight (kDa)

8.67

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 95 - 270 6.6e-46 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 1 cut(s) 740
AccII CGCG 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 2 cut(s) 492, 727
AclWI GGATC 3 cut(s) 10, 12, 23
AcsI RAATTY 1 cut(s) 503
AcvI CACGTG 1 cut(s) 686
AcyI GRCGYC 2 cut(s) 62, 384
AfaI GTAC 4 cut(s) 311, 317, 500, 674
AfiI CCNNNNNNNGG 2 cut(s) 247, 491
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 6 cut(s) 92, 139, 160, 302, 447, 679
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 4 cut(s) 142, 255, 634, 767
AluI AGCT 4 cut(s) 142, 255, 634, 767
Alw21I GWGCWC 3 cut(s) 441, 582, 719
Alw26I GTCTC 1 cut(s) 530
Alw44I GTGCAC 1 cut(s) 437
AlwI GGATC 3 cut(s) 10, 12, 23
AlwNI CAGNNNCTG 1 cut(s) 539
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 238, 397
ApaLI GTGCAC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 767, 770
ApoI RAATTY 1 cut(s) 503
AspLEI GCGC 2 cut(s) 386, 763
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 3 cut(s) 262, 295, 473
AsuNHI GCTAGC 1 cut(s) 763
BaeGI GKGCMC 1 cut(s) 441
BamHI GGATCC 2 cut(s) 4, 15
BanI GGYRCC 1 cut(s) 383
BbrPI CACGTG 1 cut(s) 686
Bbv12I GWGCWC 3 cut(s) 441, 582, 719
BbvI GCAGC 2 cut(s) 754, 757
BcgI CGANNNNNNTGC 4 cut(s) 261, 295, 763, 797
BciT130I CCWGG 1 cut(s) 423
BcnI CCSGG 1 cut(s) 396
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 2 cut(s) 605, 764
BfoI RGCGCY 1 cut(s) 387
BisI GCNGC 2 cut(s) 768, 771
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 2 cut(s) 769, 772
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 2 cut(s) 396, 423
BmiI GGNNCC 3 cut(s) 6, 17, 385
BmrFI CCNGG 2 cut(s) 396, 423
BmsI GCATC 1 cut(s) 281
BmtI GCTAGC 1 cut(s) 767
Bpu1102I GCTNAGC 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 396
BsaAI YACGTR 3 cut(s) 39, 319, 686
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 2 cut(s) 62, 384
BsaJI CCNNGG 1 cut(s) 422
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 491
Bse8I GATNNNNATC 1 cut(s) 600
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 1 cut(s) 391
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 2 cut(s) 247, 491
BseMII CTCAG 1 cut(s) 621
BseRI GAGGAG 3 cut(s) 112, 115, 118
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 2 cut(s) 754, 757
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 2 cut(s) 240, 399
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 3 cut(s) 441, 582, 719
BsiSI CCGG 2 cut(s) 8, 396
BslI CCNNNNNNNGG 2 cut(s) 247, 491
BsmAI GTCTC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 2 cut(s) 240, 399
Bsp1286I GDGCHC 3 cut(s) 441, 582, 719
Bsp13I TCCGGA 1 cut(s) 7
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 5 cut(s) 4, 15, 267, 364, 388
Bsp1720I GCTNAGC 1 cut(s) 630
BspACI CCGC 2 cut(s) 492, 727
BspANI GGCC 2 cut(s) 240, 399
BspCNI CTCAG 1 cut(s) 622
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 6, 17, 385
BspOI GCTAGC 1 cut(s) 767
BspPI GGATC 3 cut(s) 10, 12, 23
BspT107I GGYRCC 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 5 cut(s) 4, 15, 267, 364, 388
BssNAI GTATAC 1 cut(s) 741
BssNI GRCGYC 2 cut(s) 62, 384
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 5 cut(s) 289, 340, 436, 541, 745
BstACI GRCGYC 2 cut(s) 62, 384
BstAUI TGTACA 1 cut(s) 498
BstBAI YACGTR 3 cut(s) 39, 319, 686
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 2 cut(s) 630, 635
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 2 cut(s) 386, 763
BstKTI GATC 5 cut(s) 7, 18, 270, 367, 391
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 5 cut(s) 4, 15, 267, 364, 388
BstMWI GCNNNNNNNGC 2 cut(s) 767, 776
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 394, 421
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 754, 757
BstX2I RGATCY 2 cut(s) 4, 15
BstYI RGATCY 2 cut(s) 4, 15
BstZ17I GTATAC 1 cut(s) 741
BsuRI GGCC 2 cut(s) 240, 399
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 294, 750
Cac8I GCNNGC 1 cut(s) 765
CaiI CAGNNNCTG 1 cut(s) 539
CfoI GCGC 2 cut(s) 386, 763
CseI GACGC 1 cut(s) 238
Csp6I GTAC 4 cut(s) 310, 316, 499, 673
CviAII CATG 1 cut(s) 557
CviJI RGCY 7 cut(s) 142, 240, 255, 399, 482, 634, 767
CviKI_1 RGCY 7 cut(s) 142, 240, 255, 399, 482, 634, 767
CviQI GTAC 4 cut(s) 310, 316, 499, 673
DdeI CTNAG 2 cut(s) 630, 635
DinI GGCGCC 1 cut(s) 385
DpnI GATC 5 cut(s) 6, 17, 269, 366, 390
DpnII GATC 5 cut(s) 4, 15, 267, 364, 388
DraI TTTAAA 1 cut(s) 478
Eco72I CACGTG 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 421
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
FaeI CATG 1 cut(s) 560
FaiI YATR 7 cut(s) 104, 554, 558, 567, 705, 741, 826
FatI CATG 1 cut(s) 556
FauI CCCGC 1 cut(s) 720
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 2 cut(s) 768, 771
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 768, 771
FspBI CTAG 2 cut(s) 605, 764
GlaI GCGC 2 cut(s) 385, 762
GluI GCNGC 2 cut(s) 768, 771
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 2 cut(s) 240, 399
HapII CCGG 2 cut(s) 8, 396
HgaI GACGC 1 cut(s) 238
HhaI GCGC 2 cut(s) 386, 763
Hin1I GRCGYC 2 cut(s) 62, 384
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 2 cut(s) 384, 761
HinP1I GCGC 2 cut(s) 384, 761
HinfI GANTC 1 cut(s) 650
HpaII CCGG 2 cut(s) 8, 396
HphI GGTGA 3 cut(s) 262, 295, 473
Hpy166II GTNNAC 6 cut(s) 70, 292, 310, 439, 741, 793
Hpy188I TCNGA 2 cut(s) 169, 263
Hpy188III TCNNGA 4 cut(s) 8, 368, 412, 605
Hpy8I GTNNAC 6 cut(s) 70, 292, 310, 439, 741, 793
Hpy99I CGWCG 3 cut(s) 263, 299, 365
HpyAV CCTTC 4 cut(s) 122, 207, 251, 323
HpyCH4III ACNGT 5 cut(s) 289, 340, 436, 541, 745
HpyCH4IV ACGT 5 cut(s) 38, 62, 297, 318, 685
HpyCH4V TGCA 3 cut(s) 439, 784, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 767, 776
HpyF3I CTNAG 2 cut(s) 630, 635
HpySE526I ACGT 5 cut(s) 38, 62, 297, 318, 685
Hsp92I GRCGYC 2 cut(s) 62, 384
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 2 cut(s) 384, 761
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 5 cut(s) 4, 15, 267, 364, 388
LpnPI CCDG 7 cut(s) 21, 364, 408, 409, 435, 444, 546
Lsp1109I GCAGC 2 cut(s) 754, 757
LweI GCATC 1 cut(s) 281
MaeI CTAG 2 cut(s) 605, 764
MaeII ACGT 5 cut(s) 38, 62, 297, 318, 685
MaeIII GTNAC 3 cut(s) 283, 412, 655
MalI GATC 5 cut(s) 6, 17, 269, 366, 390
MboI GATC 5 cut(s) 4, 15, 267, 364, 388
MflI RGATCY 2 cut(s) 4, 15
MhlI GDGCHC 3 cut(s) 441, 582, 719
MluCI AATT 2 cut(s) 503, 624
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 156, 241
MnlI CCTC 9 cut(s) 6, 128, 133, 136, 139, 202, 213, 410, 717
MroI TCCGGA 1 cut(s) 7
MseI TTAA 4 cut(s) 407, 477, 549, 621
MslI CAYNNNNRTG 1 cut(s) 708
MspI CCGG 2 cut(s) 8, 396
MspR9I CCNGG 2 cut(s) 396, 423
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 423
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 767, 776
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 1 cut(s) 396
NdeII GATC 5 cut(s) 4, 15, 267, 364, 388
NheI GCTAGC 1 cut(s) 763
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 3 cut(s) 6, 17, 385
NmuCI GTSAC 2 cut(s) 283, 412
NspI RCATGY 1 cut(s) 560
PciI ACATGT 1 cut(s) 556
PcsI WCGNNNNNNNCGW 7 cut(s) 35, 303, 324, 360, 366, 408, 691
PctI GAATGC 1 cut(s) 308
PflFI GACNNNGTC 1 cut(s) 699
PkrI GCNGC 2 cut(s) 769, 772
PleI GAGTC 1 cut(s) 658
PluTI GGCGCC 1 cut(s) 387
PmaCI CACGTG 1 cut(s) 686
PmlI CACGTG 1 cut(s) 686
PpsI GAGTC 1 cut(s) 658
Ppu21I YACGTR 3 cut(s) 39, 319, 686
PscI ACATGT 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 421
PspCI CACGTG 1 cut(s) 686
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 3 cut(s) 6, 17, 385
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 2 cut(s) 4, 15
PsyI GACNNNGTC 1 cut(s) 699
RsaI GTAC 4 cut(s) 311, 317, 500, 674
RsaNI GTAC 4 cut(s) 310, 316, 499, 673
RseI CAYNNNNRTG 1 cut(s) 708
SaqAI TTAA 4 cut(s) 407, 477, 549, 621
SatI GCNGC 2 cut(s) 768, 771
Sau3AI GATC 5 cut(s) 4, 15, 267, 364, 388
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 2 cut(s) 396, 423
SduI GDGCHC 3 cut(s) 441, 582, 719
SfaNI GCATC 1 cut(s) 281
SfoI GGCGCC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 708
Sse9I AATT 2 cut(s) 503, 624
SsiI CCGC 2 cut(s) 492, 727
SspDI GGCGCC 1 cut(s) 383
SspI AATATT 2 cut(s) 163, 586
SspMI CTAG 2 cut(s) 605, 764
StyD4I CCNGG 2 cut(s) 394, 421
TaaI ACNGT 5 cut(s) 289, 340, 436, 541, 745
TaiI ACGT 5 cut(s) 41, 65, 300, 321, 688
TaqI TCGA 4 cut(s) 29, 363, 402, 694
TasI AATT 2 cut(s) 503, 624
TatI WGTACW 2 cut(s) 498, 672
Tru1I TTAA 4 cut(s) 407, 477, 549, 621
Tru9I TTAA 4 cut(s) 407, 477, 549, 621
TscAI CASTG 2 cut(s) 294, 750
TseFI GTSAC 2 cut(s) 283, 412
TseI GCWGC 2 cut(s) 767, 770
Tsp45I GTSAC 2 cut(s) 283, 412
TspDTI ATGAA 1 cut(s) 612
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 2 cut(s) 294, 750
Tth111I GACNNNGTC 1 cut(s) 699
VneI GTGCAC 1 cut(s) 437
XapI RAATTY 1 cut(s) 503
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 740
XspI CTAG 2 cut(s) 605, 764
ZraI GACGTC 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.