pycom13g20990

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002200
Physical Location & Seq
Forward (+)
17223330 .. 17226693
3364 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGGAGGTACAGACATGCGGGAAACCAATTGACTCATTGCTCGAGAAGGTGCTTTGTATGAACATTCTATCTTCCGACTACTTCAAGGAGCTTTACCGGTTAAAGACGTACCACGAAGTCATTGATGAAATTTACAACCAAGTAGACCATGTCGAGCCGTGGATGACCGGGAATTGCCGCGGTCCGTCCACTGCCTTTTGTCTGCTGTACAAGTTCTTCACCATGAAGCTCACCGTTAAGCAAATGCACGGTCTTTTAAAGCACGAGGATTCGCCTTACATAAGAGCGGTGGGGTTCCTCTACTTGAGATATGCTGCAGACCCAAAGACTTTGTGGAATTGGGTTGAACCCTATATTAAAGATGATGAGGACATTATGCGGTGTAAGGCGGCCGCCTGGGCGGGCTTGGCGGATGTTGGGCGGTCTGGGCACGACCTGGGAAAAACTTATGTCGCGAGACTCACAACCTTCTTGTCTCCCCGAGTCTTCCCGTCTGCCTCTTTGTTTGCTAGAAGAGGAGTCGAATATGGGGAGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGATTTTTGCAATTGTAGGGATGGGTGATGGCGATGGGTTGAATTCTCTCCTGGTTCCAATGGACGGATGA

Protein Analysis

211

Amino Acids

23.79

Weight (kDa)

5.97

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP38 PF03371 11 - 127 2.2e-42 PRP38 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 144
AccII CGCG 2 cut(s) 180, 455
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 2 cut(s) 129, 604
AfaI GTAC 3 cut(s) 9, 110, 209
AfiI CCNNNNNNNGG 1 cut(s) 626
AgeI ACCGGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 85, 347, 604
AjnI CCWGG 3 cut(s) 395, 435, 612
AluBI AGCT 2 cut(s) 91, 229
AluI AGCT 2 cut(s) 91, 229
Alw26I GTCTC 2 cut(s) 451, 480
Ama87I CYCGRG 2 cut(s) 41, 480
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 2 cut(s) 129, 604
ArsI GACNNNNNNTTYG 2 cut(s) 235, 267
AsiGI ACCGGT 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 182
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 211, 223, 599
AvaI CYCGRG 2 cut(s) 41, 480
AvaII GGWCC 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 432
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BauI CACGAG 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 577, 584, 590
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 397, 437, 614
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 451, 480
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 4 cut(s) 178, 315, 390, 393
BlsI GCNGC 4 cut(s) 179, 316, 391, 394
Bme1390I CCNGG 4 cut(s) 169, 397, 437, 614
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 2 cut(s) 41, 480
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 296, 618
BmrFI CCNGG 4 cut(s) 169, 397, 437, 614
BpiI GAAGAC 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 169
BsaJI CCNNGG 4 cut(s) 158, 178, 396, 436
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse118I RCCGGY 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 35
BseBI CCWGG 3 cut(s) 397, 437, 614
BseDI CCNNGG 4 cut(s) 158, 178, 396, 436
BseGI GGATG 3 cut(s) 168, 418, 588
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 1 cut(s) 35
BseRI GAGGAG 1 cut(s) 531
BseSI GKGCMC 1 cut(s) 432
BseX3I CGGCCG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 301
Bsh1236I CGCG 2 cut(s) 180, 455
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 1 cut(s) 392
BshTI ACCGGT 1 cut(s) 96
BsiEI CGRYCG 1 cut(s) 393
BsiHKCI CYCGRG 2 cut(s) 41, 480
BsiSI CCGG 2 cut(s) 97, 168
BslI CCNNNNNNNGG 1 cut(s) 626
BsmAI GTCTC 2 cut(s) 451, 480
BsnI GGCC 1 cut(s) 392
BsoBI CYCGRG 2 cut(s) 41, 480
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 207
Bsp68I TCGCGA 1 cut(s) 455
BspANI GGCC 1 cut(s) 392
BspFNI CGCG 2 cut(s) 180, 455
BspLI GGNNCC 2 cut(s) 296, 618
BspMAI CTGCAG 1 cut(s) 319
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 96
BssECI CCNNGG 4 cut(s) 158, 178, 396, 436
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst2UI CCWGG 3 cut(s) 397, 437, 614
Bst4CI ACNGT 2 cut(s) 235, 251
Bst6I CTCTTC 1 cut(s) 508
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 403
BstDSI CCRYGG 2 cut(s) 158, 178
BstF5I GGATG 3 cut(s) 168, 418, 588
BstFNI CGCG 2 cut(s) 180, 455
BstMAI GTCTC 2 cut(s) 451, 480
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 3 cut(s) 398, 407, 427
BstNI CCWGG 3 cut(s) 397, 437, 614
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 4 cut(s) 167, 395, 435, 612
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 2 cut(s) 180, 455
BstV1I GCAGC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 478
BstZI CGGCCG 1 cut(s) 390
BsuRI GGCC 1 cut(s) 392
BtgI CCRYGG 2 cut(s) 158, 178
BtgZI GCGATG 1 cut(s) 609
BtsCI GGATG 3 cut(s) 168, 418, 588
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
BtuMI TCGCGA 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 403
CciNI GCGGCCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 96
Cfr13I GGNCC 1 cut(s) 182
Cfr42I CCGCGG 1 cut(s) 181
CpoI CGGWCCG 1 cut(s) 182
Csp6I GTAC 3 cut(s) 8, 109, 208
CspAI ACCGGT 1 cut(s) 96
CspI CGGWCCG 1 cut(s) 182
CviAII CATG 3 cut(s) 15, 149, 223
CviJI RGCY 5 cut(s) 91, 157, 229, 392, 405
CviKI_1 RGCY 5 cut(s) 91, 157, 229, 392, 405
CviQI GTAC 3 cut(s) 8, 109, 208
DraI TTTAAA 1 cut(s) 258
EaeI YGGCCR 1 cut(s) 390
EagI CGGCCG 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
EciI GGCGGA 1 cut(s) 425
EclXI CGGCCG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 182
Eco52I CGGCCG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 41, 480
EcoRI GAATTC 1 cut(s) 604
EcoRII CCWGG 3 cut(s) 395, 435, 612
FaeI CATG 3 cut(s) 18, 152, 226
FatI CATG 3 cut(s) 14, 148, 222
FauI CCCGC 2 cut(s) 11, 394
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 178, 315, 390, 393
FokI GGATG 3 cut(s) 175, 425, 595
Fsp4HI GCNGC 4 cut(s) 178, 315, 390, 393
FspBI CTAG 1 cut(s) 510
GluI GCNGC 4 cut(s) 178, 315, 390, 393
HaeIII GGCC 1 cut(s) 392
HapII CCGG 2 cut(s) 97, 168
Hin1II CATG 3 cut(s) 18, 152, 226
HinfI GANTC 5 cut(s) 32, 269, 459, 483, 519
HpaII CCGG 2 cut(s) 97, 168
HphI GGTGA 3 cut(s) 211, 223, 599
Hpy166II GTNNAC 2 cut(s) 145, 189
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 43, 454
Hpy8I GTNNAC 2 cut(s) 145, 189
HpyAV CCTTC 2 cut(s) 40, 478
HpyCH4III ACNGT 2 cut(s) 235, 251
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 247, 317, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 407, 427
HpySE526I ACGT 1 cut(s) 107
Hsp92II CATG 3 cut(s) 18, 152, 226
KspI CCGCGG 1 cut(s) 181
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 9 cut(s) 110, 181, 382, 409, 411, 422, 449, 599, 626
Lsp1109I GCAGC 1 cut(s) 301
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 107
MbiI CCGCTC 1 cut(s) 287
MboII GAAGA 4 cut(s) 63, 208, 478, 525
MfeI CAATTG 2 cut(s) 27, 573
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 6 cut(s) 27, 129, 172, 337, 573, 604
MlyI GAGTC 4 cut(s) 26, 453, 492, 528
MmeI TCCRAC 1 cut(s) 99
MseI TTAA 4 cut(s) 101, 237, 257, 357
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 2 cut(s) 97, 168
MspR9I CCNGG 4 cut(s) 169, 397, 437, 614
MunI CAATTG 2 cut(s) 27, 573
MvaI CCWGG 3 cut(s) 397, 437, 614
MvnI CGCG 2 cut(s) 180, 455
MwoI GCNNNNNNNGC 3 cut(s) 398, 407, 427
NciI CCSGG 1 cut(s) 169
NlaIII CATG 3 cut(s) 18, 152, 226
NlaIV GGNNCC 2 cut(s) 296, 618
NotI GCGGCCGC 1 cut(s) 390
NruI TCGCGA 1 cut(s) 455
NspI RCATGY 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 41
PfeI GAWTC 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 149
PinAI ACCGGT 1 cut(s) 96
PkrI GCNGC 4 cut(s) 179, 316, 391, 394
PleI GAGTC 4 cut(s) 26, 453, 491, 527
PpsI GAGTC 4 cut(s) 26, 453, 491, 527
Psp6I CCWGG 3 cut(s) 395, 435, 612
PspGI CCWGG 3 cut(s) 395, 435, 612
PspN4I GGNNCC 2 cut(s) 296, 618
PspPI GGNCC 1 cut(s) 182
PstI CTGCAG 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 149
RruI TCGCGA 1 cut(s) 455
RsaI GTAC 3 cut(s) 9, 110, 209
RsaNI GTAC 3 cut(s) 8, 109, 208
Rsr2I CGGWCCG 1 cut(s) 182
RsrII CGGWCCG 1 cut(s) 182
SacII CCGCGG 1 cut(s) 181
SaqAI TTAA 4 cut(s) 101, 237, 257, 357
SatI GCNGC 4 cut(s) 178, 315, 390, 393
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 4 cut(s) 26, 453, 492, 528
ScrFI CCNGG 4 cut(s) 169, 397, 437, 614
SduI GDGCHC 1 cut(s) 432
SetI ASST 7 cut(s) 9, 51, 93, 110, 231, 438, 470
SfcI CTRYAG 1 cut(s) 315
Sfr274I CTCGAG 1 cut(s) 41
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SinI GGWCC 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 41, 304
SmoI CTYRAG 2 cut(s) 41, 304
Sse9I AATT 6 cut(s) 27, 129, 172, 337, 573, 604
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 4 cut(s) 167, 395, 435, 612
TaaI ACNGT 2 cut(s) 235, 251
TaiI ACGT 1 cut(s) 110
TaqI TCGA 3 cut(s) 42, 153, 522
TasI AATT 6 cut(s) 27, 129, 172, 337, 573, 604
TatI WGTACW 1 cut(s) 207
TauI GCSGC 3 cut(s) 180, 392, 395
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 4 cut(s) 101, 237, 257, 357
Tru9I TTAA 4 cut(s) 101, 237, 257, 357
TscAI CASTG 1 cut(s) 196
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 74, 141, 239
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 196
Tth111I GACNNNGTC 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 2 cut(s) 129, 604
XceI RCATGY 1 cut(s) 18
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XhoI CTCGAG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.