Rh4DG078900

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002200
Physical Location & Seq
Forward (+)
11709102 .. 11714305
5204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG078900.1

Sequence Viewer

Length: 930 bp
ATGAGCGGTTGGGTGCATCTTGGAGTCGGAATCTCAAAACTACAACTAAAATTCTCTCCAGGTTCCAATGGACGAATGACCACTATGGGTGTATATGTGCGTGATTTGCTACTTGGGCAGTACTACTTTGATACACTTTTCCCTCGCATACCTGTTCCTGTGTTGCGGCAGATTACAGCTAATCTTGAGAAGATGAAACTCCCAACAAAACTCTCTGGTGTTACTGGGGAGGCTACCCGTCATGGATCTGATGACACTGCCCGTCGCCCACCTTCTGTGAAAGCGGCACTTTCAGTCTCCTTTGGTCAACGTGCCCCACACCGTGCTTCGACGAGGGACTCATCACCTGTTCGTCGAACTTTACCGCCACCGCCACCTTATGAAAAAGCTAGCACTGATGATTTACGGAGCCGTCGTAGCCAGAGCCGTGAGTATTCTGACAGAGACTATTCGGACAGGGACAGGGATAGGGGAAGGGACCGGGATCGGAATCGGGAAAGAGACAGGGATAGTGATCGTGAACGAGAAAGGTACAAGGAACGGGAGAGGGGTCGAGATAGAGACAGATCAAGGGATAGAGAACGAAGTTCTGATTATGATAGGAGGTCAAGATATGCAGAGAGAGAAAGCCGGAGGGACTATTATGAGAGTAGCCGTGATGGTGGTAGACATTCCCGTAGGAGTAGGAGTCGAAGCCGCAGTAGAAGCAGGAGTAGGAGTCGGAGCTTGCAAGCTGGCACTGGTCCTCATGTAAGCCCGCCGAGAGATGTAAACAAGGATAGAACATCTGTATCTAGCAATCTGGCAAAACTTAGGGATCTTTATGGTGATGTGAGTGTCCAAAAAGGGGATACTAGTATGGATAGGATTCCGAGGAGCGATAACGGTGCTGAAGAGGTGATTAGGCTTGGTGGTCACCGATGGAAATAG

Protein Analysis

309

Amino Acids

35.8

Weight (kDa)

10.91

Isoelectric Point (pI)

73.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP38_assoc PF12871 79 - 165 5.7e-09 Pre-mRNA-splicing factor 38-associated hydrophilic C-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 667
AciI CCGC 7 cut(s) 6, 166, 284, 365, 371, 697, 758
AclWI GGATC 3 cut(s) 253, 492, 825
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 912
AdeI CACNNNGTG 1 cut(s) 323
AfaI GTAC 2 cut(s) 122, 533
AfiI CCNNNNNNNGG 1 cut(s) 847
AhlI ACTAGT 1 cut(s) 854
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 4 cut(s) 179, 389, 726, 734
AluI AGCT 4 cut(s) 179, 389, 726, 734
Alw26I GTCTC 4 cut(s) 301, 438, 495, 555
AlwI GGATC 3 cut(s) 253, 492, 825
ApoI RAATTY 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 478, 743
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 4 cut(s) 336, 839, 908, 910
AsuNHI GCTAGC 1 cut(s) 389
AvaII GGWCC 2 cut(s) 478, 743
BaeGI GKGCMC 1 cut(s) 316
BaeI ACNNNNGTAYC 2 cut(s) 774, 807
BccI CCATC 2 cut(s) 653, 915
BceAI ACGGC 3 cut(s) 396, 411, 639
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 1 cut(s) 60
BciVI GTATCC 1 cut(s) 844
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 4 cut(s) 301, 438, 495, 555
BcuI ACTAGT 1 cut(s) 854
BfaI CTAG 3 cut(s) 390, 795, 855
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 3 cut(s) 167, 285, 697
BlsI GCNGC 3 cut(s) 168, 286, 698
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 60, 482
Bme18I GGWCC 2 cut(s) 478, 743
BmgT120I GGNCC 2 cut(s) 478, 743
BmiI GGNNCC 3 cut(s) 64, 410, 479
BmrFI CCNGG 2 cut(s) 60, 482
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 25
BmtI GCTAGC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 482
BsaBI GATNNNNATC 2 cut(s) 489, 513
BsaJI CCNNGG 1 cut(s) 872
BsaXI ACNNNNNCTCC 6 cut(s) 400, 430, 673, 703, 703, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 847
Bse1I ACTGG 2 cut(s) 229, 745
Bse8I GATNNNNATC 2 cut(s) 489, 513
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 872
BseJI GATNNNNATC 2 cut(s) 489, 513
BseLI CCNNNNNNNGG 1 cut(s) 847
BseNI ACTGG 2 cut(s) 229, 745
BseRI GAGGAG 1 cut(s) 889
BseSI GKGCMC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 481, 631
BslFI GGGAC 4 cut(s) 350, 473, 491, 650
BslI CCNNNNNNNGG 1 cut(s) 847
BsmAI GTCTC 4 cut(s) 301, 438, 495, 555
BsmFI GGGAC 4 cut(s) 350, 473, 491, 650
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 5 cut(s) 245, 484, 514, 566, 817
BspACI CCGC 7 cut(s) 6, 166, 284, 365, 371, 697, 758
BspLI GGNNCC 3 cut(s) 64, 410, 479
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 3 cut(s) 253, 492, 825
BsrBI CCGCTC 1 cut(s) 6
BsrI ACTGG 2 cut(s) 229, 745
BssECI CCNNGG 1 cut(s) 872
BssMI GATC 5 cut(s) 245, 484, 514, 566, 817
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 323, 887
Bst6I CTCTTC 1 cut(s) 888
BstC8I GCNNGC 5 cut(s) 391, 728, 732, 736, 758
BstDEI CTNAG 1 cut(s) 812
BstEII GGTNACC 1 cut(s) 914
BstKTI GATC 5 cut(s) 248, 487, 517, 569, 820
BstMAI GTCTC 4 cut(s) 301, 438, 495, 555
BstMBI GATC 5 cut(s) 245, 484, 514, 566, 817
BstMWI GCNNNNNNNGC 4 cut(s) 106, 115, 417, 705
BstNI CCWGG 1 cut(s) 60
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 2 cut(s) 58, 480
BstSLI GKGCMC 1 cut(s) 316
BstX2I RGATCY 2 cut(s) 245, 817
BstYI RGATCY 2 cut(s) 245, 817
BsuI GTATCC 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 255, 393, 738
Cac8I GCNNGC 5 cut(s) 391, 728, 732, 736, 758
Cfr13I GGNCC 2 cut(s) 478, 743
Csp6I GTAC 2 cut(s) 121, 532
CviAII CATG 2 cut(s) 242, 749
CviQI GTAC 2 cut(s) 121, 532
DdeI CTNAG 1 cut(s) 812
DpnI GATC 5 cut(s) 247, 486, 516, 568, 819
DpnII GATC 5 cut(s) 245, 484, 514, 566, 817
DraIII CACNNNGTG 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Eco47I GGWCC 2 cut(s) 478, 743
Eco57I CTGAAG 1 cut(s) 912
Eco91I GGTNACC 1 cut(s) 914
EcoO65I GGTNACC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 58
FaeI CATG 2 cut(s) 245, 752
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 4 cut(s) 350, 473, 491, 650
FatI CATG 2 cut(s) 241, 748
FauI CCCGC 1 cut(s) 765
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 3 cut(s) 167, 285, 697
Fsp4HI GCNGC 3 cut(s) 167, 285, 697
FspBI CTAG 3 cut(s) 390, 795, 855
GluI GCNGC 3 cut(s) 167, 285, 697
GsuI CTGGAG 1 cut(s) 42
HapII CCGG 2 cut(s) 481, 631
Hin1II CATG 2 cut(s) 245, 752
HincII GTYRAC 1 cut(s) 308
HindII GTYRAC 1 cut(s) 308
HinfI GANTC 7 cut(s) 24, 30, 338, 490, 688, 718, 868
HpaII CCGG 2 cut(s) 481, 631
HphI GGTGA 4 cut(s) 336, 839, 908, 910
Hpy166II GTNNAC 4 cut(s) 308, 521, 668, 772
Hpy188I TCNGA 8 cut(s) 29, 250, 439, 454, 489, 592, 723, 873
Hpy188III TCNNGA 5 cut(s) 185, 494, 518, 554, 609
Hpy8I GTNNAC 4 cut(s) 308, 521, 668, 772
Hpy99I CGWCG 4 cut(s) 267, 334, 357, 417
HpyAV CCTTC 2 cut(s) 282, 468
HpyCH4III ACNGT 2 cut(s) 323, 887
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 3 cut(s) 16, 617, 730
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 115, 417, 705
HpyF3I CTNAG 1 cut(s) 812
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 2 cut(s) 245, 752
Kzo9I GATC 5 cut(s) 245, 484, 514, 566, 817
LmnI GCTCC 3 cut(s) 408, 723, 876
LweI GCATC 1 cut(s) 25
MaeI CTAG 3 cut(s) 390, 795, 855
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 220, 914
MalI GATC 5 cut(s) 247, 486, 516, 568, 819
MbiI CCGCTC 1 cut(s) 6
MboI GATC 5 cut(s) 245, 484, 514, 566, 817
MboII GAAGA 2 cut(s) 202, 905
MflI RGATCY 2 cut(s) 245, 817
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 1 cut(s) 50
MlyI GAGTC 4 cut(s) 33, 332, 697, 727
MmeI TCCRAC 2 cut(s) 7, 701
MnlI CCTC 9 cut(s) 153, 223, 327, 540, 597, 627, 756, 867, 889
MspI CCGG 2 cut(s) 481, 631
MspR9I CCNGG 2 cut(s) 60, 482
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 106, 115, 417, 705
NciI CCSGG 1 cut(s) 482
NdeII GATC 5 cut(s) 245, 484, 514, 566, 817
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 2 cut(s) 245, 752
NlaIV GGNNCC 3 cut(s) 64, 410, 479
NmeAIII GCCGAG 1 cut(s) 786
NmuCI GTSAC 1 cut(s) 914
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PfeI GAWTC 3 cut(s) 30, 490, 868
PkrI GCNGC 3 cut(s) 168, 286, 698
PleI GAGTC 4 cut(s) 32, 332, 696, 726
PpsI GAGTC 4 cut(s) 32, 332, 696, 726
Psp6I CCWGG 1 cut(s) 58
PspEI GGTNACC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 64, 410, 479
PspPI GGNCC 2 cut(s) 478, 743
PsuI RGATCY 2 cut(s) 245, 817
RsaI GTAC 2 cut(s) 122, 533
RsaNI GTAC 2 cut(s) 121, 532
SatI GCNGC 3 cut(s) 167, 285, 697
Sau3AI GATC 5 cut(s) 245, 484, 514, 566, 817
Sau96I GGNCC 2 cut(s) 478, 743
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 4 cut(s) 33, 332, 697, 727
ScrFI CCNGG 2 cut(s) 60, 482
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 1 cut(s) 25
SinI GGWCC 2 cut(s) 478, 743
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SpeI ACTAGT 1 cut(s) 854
Sse9I AATT 1 cut(s) 50
SsiI CCGC 7 cut(s) 6, 166, 284, 365, 371, 697, 758
SspMI CTAG 3 cut(s) 390, 795, 855
StyD4I CCNGG 2 cut(s) 58, 480
TaaI ACNGT 2 cut(s) 323, 887
TaiI ACGT 1 cut(s) 313
TaqI TCGA 4 cut(s) 329, 355, 553, 691
TasI AATT 1 cut(s) 50
TatI WGTACW 1 cut(s) 120
TauI GCSGC 3 cut(s) 169, 287, 699
TfiI GAWTC 3 cut(s) 30, 490, 868
TscAI CASTG 3 cut(s) 262, 400, 745
TseFI GTSAC 1 cut(s) 914
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 2 cut(s) 209, 396
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 3 cut(s) 262, 400, 745
VpaK11BI GGWCC 2 cut(s) 478, 743
XapI RAATTY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 3 cut(s) 390, 795, 855
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.