pycom14g00950

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002388
Physical Location & Seq
Reverse (-)
655256 .. 655757
502 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 327 bp
ATGATTGTATCTGTTCGTGTGTTCAGGGTTTACAATGCCCTCGGTGTCAGTTATGTCCGTGAGGGTAAACTTGCTAAGGGAATTACGCAGTTTGAAACTGCCGTGAAGCTTCAACCGGGCTACGTCACAGCTTGGAACAACCTAGGTGATGCTTACGAAAAGAGGAAAGACTTTAAGGCTGCACTGAACGCATTTGAAGAAGTACTACTGTTTGATCCGAATAACAAGGTAGCAATACCACGGCGGGAAACTTTGATGGAACAAGTGAAAATGTACAGAGATGTTCCGGTAAAAGCAAAGGAGAGATCATCTGACAGTATGCGTTGA

Protein Analysis

109

Amino Acids

12.4

Weight (kDa)

9.69

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 8 - 69 5.4e-08 Tetratricopeptide repeat
TPR_16 PF13432 13 - 69 4.6e-10 Tetratricopeptide repeat
TPR_11 PF13414 17 - 54 2.3e-07 TPR repeat
TPR_19 PF14559 19 - 75 3.6e-06 Tetratricopeptide repeat
TPR_2 PF07719 43 - 75 2.3e-08 Tetratricopeptide repeat
TPR_1 PF00515 43 - 75 4.4e-09 Tetratricopeptide repeat
TPR_8 PF13181 43 - 75 4.7e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 244
AclWI GGATC 1 cut(s) 209
AfaI GTAC 2 cut(s) 204, 275
AgsI TTSAA 3 cut(s) 95, 113, 197
AluBI AGCT 2 cut(s) 109, 131
AluI AGCT 2 cut(s) 109, 131
AlwI GGATC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 179
AspA2I CCTAGG 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 158
AvrII CCTAGG 1 cut(s) 142
BarI GAAGNNNNNNTAC 2 cut(s) 189, 221
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 250
BceAI ACGGC 2 cut(s) 86, 257
BcnI CCSGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 143
BisI GCNGC 1 cut(s) 180
BlnI CCTAGG 1 cut(s) 142
BlsI GCNGC 1 cut(s) 181
BmcAI AGTACT 1 cut(s) 204
Bme1390I CCNGG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 40, 142, 239
BsaWI WCCGGW 1 cut(s) 286
BseDI CCNNGG 3 cut(s) 40, 142, 239
BseXI GCAGC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 116, 287
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 2 cut(s) 214, 305
BspACI CCGC 1 cut(s) 244
BspPI GGATC 1 cut(s) 209
BsrGI TGTACA 1 cut(s) 273
BssECI CCNNGG 3 cut(s) 40, 142, 239
BssMI GATC 2 cut(s) 214, 305
BssT1I CCWWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 210, 317
BstAUI TGTACA 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 75
BstDSI CCRYGG 1 cut(s) 239
BstKTI GATC 2 cut(s) 217, 308
BstMBI GATC 2 cut(s) 214, 305
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 166
BtgI CCRYGG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 182
Csp6I GTAC 2 cut(s) 203, 274
CviJI RGCY 4 cut(s) 109, 120, 131, 179
CviKI_1 RGCY 4 cut(s) 109, 120, 131, 179
CviQI GTAC 2 cut(s) 203, 274
DdeI CTNAG 1 cut(s) 75
DpnI GATC 2 cut(s) 216, 307
DpnII GATC 2 cut(s) 214, 305
Eco130I CCWWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
FaiI YATR 2 cut(s) 54, 320
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 180
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 1 cut(s) 143
GluI GCNGC 1 cut(s) 180
HapII CCGG 2 cut(s) 116, 287
HindIII AAGCTT 1 cut(s) 107
HpaII CCGG 2 cut(s) 116, 287
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 31, 68
Hpy188I TCNGA 2 cut(s) 219, 313
Hpy8I GTNNAC 2 cut(s) 31, 68
HpyCH4III ACNGT 2 cut(s) 210, 317
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 1 cut(s) 123
Kzo9I GATC 2 cut(s) 214, 305
LpnPI CCDG 3 cut(s) 10, 129, 300
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 1 cut(s) 139
MaeI CTAG 1 cut(s) 143
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 124
MalI GATC 2 cut(s) 216, 307
MboI GATC 2 cut(s) 214, 305
MboII GAAGA 1 cut(s) 209
MluCI AATT 1 cut(s) 81
MnlI CCTC 3 cut(s) 50, 55, 156
MseI TTAA 1 cut(s) 174
MspI CCGG 2 cut(s) 116, 287
MspR9I CCNGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 188
NciI CCSGG 1 cut(s) 117
NdeII GATC 2 cut(s) 214, 305
NmuCI GTSAC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 181
RsaI GTAC 2 cut(s) 204, 275
RsaNI GTAC 2 cut(s) 203, 274
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 2 cut(s) 214, 305
ScaI AGTACT 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 117
SetI ASST 6 cut(s) 111, 126, 133, 144, 148, 231
SfaNI GCATC 1 cut(s) 139
Sse9I AATT 1 cut(s) 81
SsiI CCGC 1 cut(s) 244
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 210, 317
TaiI ACGT 1 cut(s) 126
TasI AATT 1 cut(s) 81
TatI WGTACW 2 cut(s) 202, 273
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 124
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 189
XmaJI CCTAGG 1 cut(s) 142
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.