pycom14g02130

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004631
Physical Location & Seq
Reverse (-)
1661839 .. 1663626
1788 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGCTTCTCAAATTCTGGCAGCGGCAGCCGAGAGGATCGGACCGGCGGCGAGGCGACAGGCTGTGAGCTTGACGGACGCGGCATCGAATCGGATAAGGCAGCTCCTAGAGCAGCGCCGGCGGCCATATCTTAAACTCGGCGTCAAGGCTCGCGGCTGCAACGGTTTGTCCTACACTCTCAATTACGCAGATGATAAGGGGAAGTTTGACGAATTGGTGGAGGATAAAGGTGTGAAGGTTTTGATCGATCCGAAGGCTCTGATGCACGTCATAGGAACGAAGATGGATTTTATAAATGATAAACTCAGGTCTGAGTTTGTATTTATCAATCCCAACTCTAAAGGACAGTGTGGTTGCGGGGAATCGTTCATGACAACAGGCAGTAGCGGAGCTACGAAGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

136

Amino Acids

14.74

Weight (kDa)

9.64

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 22 - 121 1.2e-17 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccII CGCG 2 cut(s) 80, 153
AciI CCGC 7 cut(s) 23, 47, 80, 121, 153, 357, 387
AclWI GGATC 2 cut(s) 44, 242
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 141
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 3 cut(s) 69, 103, 392
AluI AGCT 3 cut(s) 69, 103, 392
AlwI GGATC 2 cut(s) 44, 242
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 5 cut(s) 20, 26, 100, 112, 156
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 41
AvaII GGWCC 1 cut(s) 41
BbvI GCAGC 5 cut(s) 32, 38, 112, 124, 143
BccI CCATC 1 cut(s) 277
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 41
BmsI GCATC 2 cut(s) 92, 252
Bsa29I ATCGAT 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 141
Bse118I RCCGGY 2 cut(s) 43, 117
BseCI ATCGAT 1 cut(s) 246
BseMII CTCAG 2 cut(s) 303, 319
BseXI GCAGC 5 cut(s) 32, 38, 112, 124, 143
Bsh1236I CGCG 2 cut(s) 80, 153
BshFI GGCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 2 cut(s) 44, 118
BsnI GGCC 1 cut(s) 124
Bsp143I GATC 3 cut(s) 36, 243, 247
BspACI CCGC 7 cut(s) 23, 47, 80, 121, 153, 357, 387
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 2 cut(s) 304, 318
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 2 cut(s) 80, 153
BspHI TCATGA 1 cut(s) 369
BspPI GGATC 2 cut(s) 44, 242
BsrFI RCCGGY 2 cut(s) 43, 117
BssAI RCCGGY 2 cut(s) 43, 117
BssMI GATC 3 cut(s) 36, 243, 247
BssNI GRCGYC 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 164, 348
BstACI GRCGYC 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 119, 151
BstDEI CTNAG 2 cut(s) 305, 312
BstFNI CGCG 2 cut(s) 80, 153
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 3 cut(s) 39, 246, 250
BstMBI GATC 3 cut(s) 36, 243, 247
BstMWI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
BstUI CGCG 2 cut(s) 80, 153
BstV1I GCAGC 5 cut(s) 32, 38, 112, 124, 143
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 1 cut(s) 124
BsuTUI ATCGAT 1 cut(s) 246
BtrI CACGTC 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 119, 151
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 2 cut(s) 43, 117
Cfr13I GGNCC 1 cut(s) 41
ClaI ATCGAT 1 cut(s) 246
CpoI CGGWCCG 1 cut(s) 41
CseI GACGC 2 cut(s) 86, 130
CspI CGGWCCG 1 cut(s) 41
CviAII CATG 1 cut(s) 370
DdeI CTNAG 2 cut(s) 305, 312
DpnI GATC 3 cut(s) 38, 245, 249
DpnII GATC 3 cut(s) 36, 243, 247
EaeI YGGCCR 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 41
FaeI CATG 1 cut(s) 373
FaiI YATR 4 cut(s) 127, 272, 293, 371
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 350
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 124
HapII CCGG 2 cut(s) 44, 118
HgaI GACGC 2 cut(s) 86, 130
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 88, 362
HpaII CCGG 2 cut(s) 44, 118
Hpy188I TCNGA 5 cut(s) 41, 93, 252, 261, 313
Hpy188III TCNNGA 1 cut(s) 370
HpyAV CCTTC 3 cut(s) 229, 247, 394
HpyCH4III ACNGT 2 cut(s) 164, 348
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 2 cut(s) 159, 265
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
HpyF3I CTNAG 2 cut(s) 305, 312
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 36, 243, 247
LmnI GCTCC 2 cut(s) 108, 389
LpnPI CCDG 6 cut(s) 2, 44, 57, 131, 292, 363
Lsp1109I GCAGC 5 cut(s) 32, 38, 112, 124, 143
LweI GCATC 2 cut(s) 92, 252
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 267
MalI GATC 3 cut(s) 38, 245, 249
MboI GATC 3 cut(s) 36, 243, 247
MboII GAAGA 1 cut(s) 292
MluCI AATT 3 cut(s) 12, 181, 212
MnlI CCTC 3 cut(s) 27, 45, 214
MreI CGCCGGCG 1 cut(s) 117
MroNI GCCGGC 1 cut(s) 117
MseI TTAA 2 cut(s) 132, 406
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 44, 118
MvnI CGCG 2 cut(s) 80, 153
MwoI GCNNNNNNNGC 4 cut(s) 26, 109, 118, 121
NaeI GCCGGC 1 cut(s) 119
NdeII GATC 3 cut(s) 36, 243, 247
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 1 cut(s) 373
NmeAIII GCCGAG 2 cut(s) 55, 117
PagI TCATGA 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 88, 362
PsiI TTATAA 1 cut(s) 293
PspPI GGNCC 1 cut(s) 41
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
SaqAI TTAA 2 cut(s) 132, 406
Sau3AI GATC 3 cut(s) 36, 243, 247
Sau96I GGNCC 1 cut(s) 41
SetI ASST 8 cut(s) 71, 105, 232, 240, 270, 311, 394, 405
SfaNI GCATC 2 cut(s) 92, 252
SgrAI CRCCGGYG 1 cut(s) 117
SinI GGWCC 1 cut(s) 41
Sse9I AATT 3 cut(s) 12, 181, 212
SsiI CCGC 7 cut(s) 23, 47, 80, 121, 153, 357, 387
SspMI CTAG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 164, 348
TaiI ACGT 1 cut(s) 270
TaqI TCGA 2 cut(s) 86, 246
TasI AATT 3 cut(s) 12, 181, 212
TauI GCSGC 5 cut(s) 26, 50, 83, 124, 156
TfiI GAWTC 2 cut(s) 88, 362
Tru1I TTAA 2 cut(s) 132, 406
Tru9I TTAA 2 cut(s) 132, 406
TscAI CASTG 1 cut(s) 353
TseI GCWGC 5 cut(s) 20, 26, 100, 112, 156
TspDTI ATGAA 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 12
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.