pycom14g04380

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003503
Physical Location & Seq
Forward (+)
3808964 .. 3811448
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1716 bp
ATGGATATGGATGTGCCACTTGTCAAAGATAGGTTAAGTTTGTTTGACAAGTTGTTAGCCGAGTTAGTTAGGGTTACAATCAATGTAGTCCCTGAAAATAGGTGTCGCAAATCAATGTGGTGCTACATAAATGGGTCTCATAAGTCTTTTTTTTTTTTTTTCCTTCACAAATGGACATCAAAATGGTTCATGAAATTGAAAATCGATAATGTAGTCCCACAAGTAAGTGTCCTAGATCAATGTAGTTTTTCCATCACAATTTTATCAAAAATTCTGTTATGTATTGGTGTAACACGCAAATGGAGGCTTAATAATTTAATAGTTAATAAAAAGTTGTCAACCCAAAAATCCAGTCATGCAATCACCTCCAATCCCAGTCCACATTCCTCTACCTCCTTCACTGTCTATTCTCTAAAAAAAAATCTTTACACACTTCGACTTCACCGAATTGAACCTGGATTGAGATCATCTTTGGTGATGGCTTGGCCAATTGGCAATGCTTGGAGAGCTTGTTCGGCGCTTCCTTGGTGGCTTGGTGACTCAAAGAATGGCGAGGTCTACCTTGTCTTGGTTAAGCCTTGTGCATTTGAGAATTTATGGAAGGGATCTCTCTTCGGAGTAGGCCCTATTATAAAAGAAAAAATTTCATTTTACAAAAAGCACATAACAAATTTTTTCTTACAGAATTGTAGTAGAATGATCATATTGATTTGCGACACCTATTTTCAAGGACTACATTCATCGATTTTCAATTTTAGGGACCATCTTGATGGTGTGGTGGGGCCCATTTATGTGCCACATCAGCACTTAATGAAATTTTTAATAGAATTTTTCAATTTCAAGGACCATCTTGATGGTGAGGATCATCCAACTCAGACTTTTCCTCTAATAGAAACATTAAATCATCAATGCACAAATCCAAGATTCTTGAGCTCAATCAGAGATTCTGGAGCACAATCGGAATCTTGTAAGCCACTGCAACCATCTCCATCTTCTCTCTTTTCTTATTCAAACCATTGTTTTTTAATATATTTACATGGACTGATTTTTCTTATTTTCAATTTTTGTTTTTGTATATGCTTTCAAGTTGGTTGTCGTTTGGAACTATGCATCCCTATTTTATTGCGAAGCGATCACAAGGGTCGCATGATCGTTGCTAAAATTTATTCACGACGGTTTCATGATTTTACACAACGAAATTTTAGCGTCACAACTTGTTATGCCAATCCTGGTCTTAAAACTCTTTTGTATATACTTTCTCTCCTTCAAAACGCTCTACATAATCCTCGGTTCTCTACAATGATCAAGACCCTCCAAAACCTCAGTTTTACCTGCACCATTCTCATAACCATTTCCTTCTTCGTCCTCTCAACCCTTGTCGCATCGGAAACTCACCGTTTTATAAGACCGTTATCTTCATCAAAACATGGTATAAAGAGAGAGAAACTAAGCCATATTCACTTCTACTTCCATGACATAGTCAGTGGCCGACCCCCACGGACTGGCTTTGGCACTGTGTTCATGACGGACGATCCATTGACTATCGGCCCTGAATCGAGCACTAAGCTAGTCGGAAAAGCCCAAGGTATTTATGCTTCAGCTTCACAAAGTGAGTTGGGGCTTTTGATGGCCTTGAATTTTGTTTTTGTTGAAGGGAAGTATATAATGGTAGCACTCTTATTGCGTTGGGGCGCAACACCGTGTCCTCAGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

572

Amino Acids

65.36

Weight (kDa)

9.48

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 484 - 554 1.2e-18 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 632, 1403
Acc36I ACCTGC 1 cut(s) 1340
AccB7I CCANNNNNTGG 1 cut(s) 1502
AccI GTMKAC 1 cut(s) 558
AclWI GGATC 3 cut(s) 613, 870, 1526
AcoI YGGCCR 2 cut(s) 485, 1486
AcsI RAATTY 9 cut(s) 270, 592, 642, 670, 815, 827, 1161, 1198, 1636
AcuI CTGAAG 1 cut(s) 1581
AdeI CACNNNGTG 1 cut(s) 1610
AfiI CCNNNNNNNGG 2 cut(s) 568, 1502
AjnI CCWGG 2 cut(s) 454, 1228
AloI GAACNNNNNNTCC 4 cut(s) 496, 528, 1095, 1127
AluBI AGCT 4 cut(s) 509, 933, 1567, 1601
AluI AGCT 4 cut(s) 509, 933, 1567, 1601
Alw21I GWGCWC 3 cut(s) 935, 955, 1562
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 3 cut(s) 613, 870, 1526
AoxI GGCC 6 cut(s) 485, 622, 782, 1486, 1546, 1629
ApaI GGGCCC 1 cut(s) 786
ApoI RAATTY 9 cut(s) 270, 592, 642, 670, 815, 827, 1161, 1198, 1636
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AspLEI GCGC 2 cut(s) 520, 1694
AspS9I GGNCC 6 cut(s) 623, 760, 782, 783, 844, 1547
AsuHPI GGTGA 6 cut(s) 355, 434, 487, 548, 869, 1385
AvaII GGWCC 2 cut(s) 760, 844
BaeGI GKGCMC 1 cut(s) 786
BalI TGGCCA 1 cut(s) 487
BanII GRGCYC 2 cut(s) 786, 935
BarI GAAGNNNNNNTAC 2 cut(s) 1579, 1611
Bbv12I GWGCWC 3 cut(s) 935, 955, 1562
BbvCI CCTCAGC 1 cut(s) 1707
BccI CCATC 9 cut(s) 260, 472, 764, 771, 848, 855, 991, 997, 1621
BceAI ACGGC 1 cut(s) 1696
BciT130I CCWGG 2 cut(s) 456, 1230
BclI TGATCA 2 cut(s) 699, 1302
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 2 cut(s) 233, 1568
BfoI RGCGCY 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 1340
Bme1390I CCNGG 2 cut(s) 456, 1230
Bme18I GGWCC 2 cut(s) 760, 844
BmgT120I GGNCC 6 cut(s) 623, 760, 782, 783, 844, 1547
BmiI GGNNCC 3 cut(s) 761, 783, 784
BmrFI CCNGG 2 cut(s) 456, 1230
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 1119, 1391
BmuI ACTGGG 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 969
Bpu10I CCTNAGC 1 cut(s) 1707
BpuEI CTTGAG 1 cut(s) 949
Bsa29I ATCGAT 2 cut(s) 204, 743
BsaBI GATNNNNATC 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 4 cut(s) 524, 1286, 1496, 1582
BsaXI ACNNNNNCTCC 4 cut(s) 496, 526, 1245, 1275
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 1502
Bse1I ACTGG 3 cut(s) 351, 375, 1507
Bse3DI GCAATG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 463
BseBI CCWGG 2 cut(s) 456, 1230
BseCI ATCGAT 2 cut(s) 204, 743
BseDI CCNNGG 4 cut(s) 524, 1286, 1496, 1582
BseGI GGATG 3 cut(s) 16, 865, 1110
BseJI GATNNNNATC 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 568, 1502
BseMI GCAATG 1 cut(s) 502
BseMII CTCAG 2 cut(s) 887, 1336
BseNI ACTGG 3 cut(s) 351, 375, 1507
BseSI GKGCMC 1 cut(s) 786
BsgI GTGCAG 1 cut(s) 1318
BshFI GGCC 6 cut(s) 487, 624, 784, 1488, 1548, 1631
BshVI ATCGAT 2 cut(s) 204, 743
BsiHKAI GWGCWC 3 cut(s) 935, 955, 1562
BslFI GGGAC 3 cut(s) 74, 200, 773
BslI CCNNNNNNNGG 2 cut(s) 568, 1502
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 3 cut(s) 74, 200, 773
BsnI GGCC 6 cut(s) 487, 624, 784, 1488, 1548, 1631
Bso31I GGTCTC 1 cut(s) 141
Bsp120I GGGCCC 1 cut(s) 782
Bsp1286I GDGCHC 4 cut(s) 786, 935, 955, 1562
Bsp143I GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
BspANI GGCC 6 cut(s) 487, 624, 784, 1488, 1548, 1631
BspCNI CTCAG 2 cut(s) 886, 1335
BspDI ATCGAT 2 cut(s) 204, 743
BspHI TCATGA 3 cut(s) 189, 1180, 1521
BspLI GGNNCC 3 cut(s) 761, 783, 784
BspMI ACCTGC 1 cut(s) 1340
BspPI GGATC 3 cut(s) 613, 870, 1526
BspTNI GGTCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 502
BsrI ACTGG 3 cut(s) 351, 375, 1507
BssECI CCNNGG 4 cut(s) 524, 1286, 1496, 1582
BssMI GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
BssT1I CCWWGG 2 cut(s) 524, 1582
Bst2UI CCWGG 2 cut(s) 456, 1230
Bst4CI ACNGT 6 cut(s) 403, 1176, 1397, 1410, 1516, 1701
Bst6I CTCTTC 1 cut(s) 617
BstDEI CTNAG 5 cut(s) 873, 1322, 1448, 1563, 1707
BstDSI CCRYGG 1 cut(s) 1496
BstF5I GGATG 3 cut(s) 16, 865, 1110
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 2 cut(s) 520, 1694
BstKTI GATC 9 cut(s) 238, 467, 608, 702, 865, 1135, 1152, 1305, 1534
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
BstMWI GCNNNNNNNGC 3 cut(s) 506, 515, 802
BstNI CCWGG 2 cut(s) 456, 1230
BstSCI CCNGG 2 cut(s) 454, 1228
BstSLI GKGCMC 1 cut(s) 786
BstX2I RGATCY 1 cut(s) 605
BstXI CCANNNNNNTGG 2 cut(s) 770, 854
BstYI RGATCY 1 cut(s) 605
Bsu15I ATCGAT 2 cut(s) 204, 743
BsuRI GGCC 6 cut(s) 487, 624, 784, 1488, 1548, 1631
BsuTUI ATCGAT 2 cut(s) 204, 743
BtgI CCRYGG 1 cut(s) 1496
BtsCI GGATG 3 cut(s) 16, 865, 1110
BtsI GCAGTG 1 cut(s) 974
BtsIMutI CAGTG 4 cut(s) 399, 974, 1489, 1512
BveI ACCTGC 1 cut(s) 1340
CciI TCATGA 3 cut(s) 189, 1180, 1521
CfoI GCGC 2 cut(s) 520, 1694
Cfr13I GGNCC 6 cut(s) 623, 760, 782, 783, 844, 1547
ClaI ATCGAT 2 cut(s) 204, 743
CseI GACGC 1 cut(s) 1195
CviAII CATG 8 cut(s) 190, 356, 1037, 1147, 1181, 1427, 1472, 1522
DdeI CTNAG 5 cut(s) 873, 1322, 1448, 1563, 1707
DpnI GATC 9 cut(s) 237, 466, 607, 701, 864, 1134, 1151, 1304, 1533
DpnII GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
DraIII CACNNNGTG 1 cut(s) 1610
EaeI YGGCCR 2 cut(s) 485, 1486
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 2 cut(s) 524, 1582
Eco24I GRGCYC 2 cut(s) 786, 935
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 760, 844
Eco53kI GAGCTC 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 1581
EcoICRI GAGCTC 1 cut(s) 933
EcoO109I RGGNCCY 2 cut(s) 623, 782
EcoRII CCWGG 2 cut(s) 454, 1228
EcoT14I CCWWGG 2 cut(s) 524, 1582
EcoT22I ATGCAT 1 cut(s) 1112
EcoT38I GRGCYC 2 cut(s) 786, 935
ErhI CCWWGG 2 cut(s) 524, 1582
FaeI CATG 8 cut(s) 193, 359, 1040, 1150, 1184, 1430, 1475, 1525
FaqI GGGAC 3 cut(s) 74, 200, 773
FatI CATG 8 cut(s) 189, 355, 1036, 1146, 1180, 1426, 1471, 1521
FbaI TGATCA 2 cut(s) 699, 1302
FblI GTMKAC 1 cut(s) 558
FokI GGATG 3 cut(s) 23, 852, 1097
FriOI GRGCYC 2 cut(s) 786, 935
FspBI CTAG 2 cut(s) 233, 1568
GlaI GCGC 2 cut(s) 519, 1693
GsuI CTGGAG 1 cut(s) 969
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 6 cut(s) 487, 624, 784, 1488, 1548, 1631
HgaI GACGC 1 cut(s) 1195
HhaI GCGC 2 cut(s) 520, 1694
Hin1II CATG 8 cut(s) 193, 359, 1040, 1150, 1184, 1430, 1475, 1525
Hin6I GCGC 2 cut(s) 518, 1692
HinP1I GCGC 2 cut(s) 518, 1692
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HinfI GANTC 5 cut(s) 539, 924, 944, 962, 1553
HphI GGTGA 6 cut(s) 355, 434, 487, 548, 869, 1385
Hpy166II GTNNAC 3 cut(s) 339, 380, 559
Hpy188I TCNGA 6 cut(s) 617, 876, 941, 961, 1387, 1574
Hpy188III TCNNGA 9 cut(s) 190, 767, 851, 928, 948, 1170, 1181, 1306, 1522
Hpy8I GTNNAC 3 cut(s) 339, 380, 559
Hpy99I CGWCG 1 cut(s) 1176
HpyAV CCTTC 6 cut(s) 173, 406, 595, 1274, 1366, 1646
HpyCH4III ACNGT 6 cut(s) 403, 1176, 1397, 1410, 1516, 1701
HpyCH4V TGCA 6 cut(s) 359, 584, 912, 979, 1110, 1335
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 515, 802
HpyF3I CTNAG 5 cut(s) 873, 1322, 1448, 1563, 1707
Hsp92II CATG 8 cut(s) 193, 359, 1040, 1150, 1184, 1430, 1475, 1525
HspAI GCGC 2 cut(s) 518, 1692
Ksp22I TGATCA 2 cut(s) 699, 1302
Kzo9I GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
LmnI GCTCC 1 cut(s) 950
LweI GCATC 2 cut(s) 1119, 1391
MaeI CTAG 2 cut(s) 233, 1568
MaeIII GTNAC 4 cut(s) 73, 289, 536, 1207
MalI GATC 9 cut(s) 237, 466, 607, 701, 864, 1134, 1151, 1304, 1533
MboI GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
MboII GAAGA 4 cut(s) 604, 984, 1351, 1407
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 4 cut(s) 786, 935, 955, 1562
MlsI TGGCCA 1 cut(s) 487
MluNI TGGCCA 1 cut(s) 487
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 893, 1552
Mox20I TGGCCA 1 cut(s) 487
Mph1103I ATGCAT 1 cut(s) 1112
MscI TGGCCA 1 cut(s) 487
MslI CAYNNNNRTG 5 cut(s) 181, 298, 768, 791, 852
Msp20I TGGCCA 1 cut(s) 487
MspR9I CCNGG 2 cut(s) 456, 1230
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 2 cut(s) 456, 1230
MwoI GCNNNNNNNGC 3 cut(s) 506, 515, 802
NdeII GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
NlaIII CATG 8 cut(s) 193, 359, 1040, 1150, 1184, 1430, 1475, 1525
NlaIV GGNNCC 3 cut(s) 761, 783, 784
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 536, 1207
NsiI ATGCAT 1 cut(s) 1112
PagI TCATGA 3 cut(s) 189, 1180, 1521
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PfeI GAWTC 4 cut(s) 924, 944, 962, 1553
PflFI GACNNNGTC 1 cut(s) 1478
PflMI CCANNNNNTGG 1 cut(s) 1502
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
PsiI TTATAA 2 cut(s) 632, 1403
Psp124BI GAGCTC 1 cut(s) 935
Psp6I CCWGG 2 cut(s) 454, 1228
PspGI CCWGG 2 cut(s) 454, 1228
PspN4I GGNNCC 3 cut(s) 761, 783, 784
PspOMI GGGCCC 1 cut(s) 782
PspPI GGNCC 6 cut(s) 623, 760, 782, 783, 844, 1547
PsuI RGATCY 1 cut(s) 605
PsyI GACNNNGTC 1 cut(s) 1478
RseI CAYNNNNRTG 5 cut(s) 181, 298, 768, 791, 852
SacI GAGCTC 1 cut(s) 935
Sau3AI GATC 9 cut(s) 235, 464, 605, 699, 862, 1132, 1149, 1302, 1531
Sau96I GGNCC 6 cut(s) 623, 760, 782, 783, 844, 1547
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 456, 1230
SduI GDGCHC 4 cut(s) 786, 935, 955, 1562
SfaNI GCATC 2 cut(s) 1119, 1391
SinI GGWCC 2 cut(s) 760, 844
SmiMI CAYNNNNRTG 5 cut(s) 181, 298, 768, 791, 852
SmlI CTYRAG 1 cut(s) 928
SmoI CTYRAG 1 cut(s) 928
SspMI CTAG 2 cut(s) 233, 1568
SstI GAGCTC 1 cut(s) 935
StyD4I CCNGG 2 cut(s) 454, 1228
StyI CCWWGG 2 cut(s) 524, 1582
TaaI ACNGT 6 cut(s) 403, 1176, 1397, 1410, 1516, 1701
TaqI TCGA 4 cut(s) 204, 436, 743, 1556
TfiI GAWTC 4 cut(s) 924, 944, 962, 1553
TscAI CASTG 4 cut(s) 406, 981, 1489, 1519
TseFI GTSAC 2 cut(s) 536, 1207
Tsp45I GTSAC 2 cut(s) 536, 1207
TspDTI ATGAA 8 cut(s) 178, 206, 636, 729, 827, 1169, 1407, 1510
TspGWI ACGGA 2 cut(s) 1513, 1541
TspRI CASTG 4 cut(s) 406, 981, 1489, 1519
Tth111I GACNNNGTC 1 cut(s) 1478
Van91I CCANNNNNTGG 1 cut(s) 1502
VpaK11BI GGWCC 2 cut(s) 760, 844
XapI RAATTY 9 cut(s) 270, 592, 642, 670, 815, 827, 1161, 1198, 1636
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 2 cut(s) 233, 1568
Zsp2I ATGCAT 1 cut(s) 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.